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Q9W4T4 (PDE4A_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I

EC=3.1.4.53
Alternative name(s):
Learning/memory process protein
Protein dunce
Gene names
Name:dnc
ORF Names:CG32498
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity. Vital for female fertility. Required for learning/memory. Ref.3

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. Ref.3

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Subunit structure

Monomer. Ref.3

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Ref.3

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandcAMP
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processassociative learning

Inferred from mutant phenotype PubMed 10970024. Source: FlyBase

axon extension

Inferred from mutant phenotype PubMed 14960616. Source: FlyBase

behavioral response to ethanol

Non-traceable author statement PubMed 11715043. Source: FlyBase

cAMP catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

cAMP-mediated signaling

Non-traceable author statement PubMed 11092827PubMed 11462211PubMed 11741030PubMed 14515157. Source: FlyBase

circadian rhythm

Traceable author statement PubMed 12486701. Source: FlyBase

conditioned taste aversion

Inferred from mutant phenotype PubMed 19158309. Source: FlyBase

courtship behavior

Traceable author statement PubMed 11092827PubMed 11715043PubMed 12486700. Source: FlyBase

lateral inhibition

Inferred from mutant phenotype PubMed 19363474. Source: FlyBase

learning

Inferred from mutant phenotype PubMed 16135747. Source: FlyBase

learning or memory

Non-traceable author statement PubMed 11715043PubMed 14515157. Source: FlyBase

locomotor rhythm

Non-traceable author statement PubMed 11715043. Source: FlyBase

mating behavior

Traceable author statement PubMed 14504657. Source: FlyBase

memory

Inferred from mutant phenotype PubMed 10970024. Source: FlyBase

nonassociative learning

Traceable author statement PubMed 14504657. Source: FlyBase

olfactory learning

Traceable author statement PubMed 11462211PubMed 11715043. Source: FlyBase

oogenesis

Traceable author statement PubMed 3089870. Source: FlyBase

reproduction

Non-traceable author statement PubMed 11715043. Source: FlyBase

short-term memory

Inferred from mutant phenotype PubMed 19158309. Source: FlyBase

synaptic transmission

Inferred from mutant phenotype PubMed 12040051PubMed 14507965. Source: FlyBase

thermosensory behavior

Inferred from mutant phenotype PubMed 18594510. Source: FlyBase

   Molecular_function3',5'-cyclic-nucleotide phosphodiesterase activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 11 isoforms produced by alternative splicing. [Align] [Select]
Isoform I Ref.3 (identifier: Q9W4T4-1)

Also known as: B; O;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform II Ref.3 (identifier: P12252-1)

Also known as: I; J;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform III (identifier: P12252-7)

Also known as: E;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation at Met-429 of isoform II.
Isoform IV Ref.3 (identifier: P12252-3)

Also known as: A;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform V Ref.3 (identifier: P12252-4)

Also known as: C;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform VI Ref.3 (identifier: P12252-5)

Also known as: D;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform VII Ref.3 (identifier: P12252-6)

Also known as: L;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform F Ref.1 (identifier: Q8IRU4-1)

The sequence of this isoform can be found in the external entry Q8IRU4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Isoform G Ref.1 (identifier: Q9W4S9-2)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Isoform M Ref.1 (identifier: Q9W4T0-1)

The sequence of this isoform can be found in the external entry Q9W4T0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Isoform N Ref.1 (identifier: Q9W4S9-1)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12091209cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I
PRO_0000198817

Regions

Nucleotide binding871 – 8755cAMP By similarity

Sites

Active site8711Proton donor By similarity
Metal binding8751Divalent metal cation 1 By similarity
Metal binding9111Divalent metal cation 1 By similarity
Metal binding9121Divalent metal cation 1 By similarity
Metal binding9121Divalent metal cation 2 By similarity
Metal binding10291Divalent metal cation 1 By similarity
Binding site9121cAMP By similarity
Binding site10291cAMP By similarity
Binding site10801cAMP By similarity
Site10321Binds AMP, but not cAMP By similarity

Experimental info

Sequence conflict10971D → S Ref.3
Sequence conflict11661S → T Ref.3
Sequence conflict1181 – 11822SG → R Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform I (B) (O) [UniParc].

Last modified July 5, 2004. Version 2.
Checksum: A7E00D5BA862C1BE

FASTA1,209129,413
        10         20         30         40         50         60 
MMRLARFTQG FSFNKNVAKH RGSGSSNGTG NGSGTGNGQG LGGSCSANGL AATGGVGGGG 

        70         80         90        100        110        120 
GSGSGGGGCG SGSGSGSGKR KSKARTKCFG GTVFRCCLPC RGGGSAAPAT SPPQTPAQTP 

       130        140        150        160        170        180 
DELKVIPEDC NLEKSAEQKR DLLERNNKED DLLNRTVSGS ELDLYGGAGG GTKKHSLADT 

       190        200        210        220        230        240 
IDTSVTTPIS LKTLINDVDE ELDQQLSAAD IAAASLASGL VARRAEPETL SDASVSPTAV 

       250        260        270        280        290        300 
VQQQQQQQQQ LQQPLLQSQP HFVPSSGNIL SQVTLYSGSN PSTNPCQSAV QNQGQNSNPN 

       310        320        330        340        350        360 
PNQNPNTNPN QNQQRCSCQP QTSPLPHIKE EEESDQANFK HQTSLKEHQP LPPPITIATG 

       370        380        390        400        410        420 
YCGSCESVHH SSATSSSAGT VPPGGQQTQE YIAGTSSTPS PRIKLKFRKP HKSCWSRIVL 

       430        440        450        460        470        480 
APIGSAGGSS SATTVIGSNS NETLASSSTT GGTATTTQNS SSVSVAAHHR LTSSSASALA 

       490        500        510        520        530        540 
TSHPSNSQLL PTSKMQAEQG SIGDLQKYHS RYLKNRRHTL ANVRFDVENG QGARSPLEGG 

       550        560        570        580        590        600 
SPSAGLVLQN LPQRRESFLY RSDSDFEMSP KSMSRNSSIA SERFKEQEAS ILVDRSHGED 

       610        620        630        640        650        660 
LIVTPFAQIL ASLRSVRNNL LSLTNVPASN KSRRPNQSSS ASRSGNPPGA PLSQGEEAYT 

       670        680        690        700        710        720 
RLATDTIEEL DWCLDQLETI QTHRSVSDMA SLKFKRMLNK ELSHFSESSR SGNQISEYIC 

       730        740        750        760        770        780 
STFLDKQQEF DLPSLRVEDN PELVAANAAA GQQSAGQYAR SRSPRGPPMS QISGVKRPLS 

       790        800        810        820        830        840 
HTNSFTGERL PTFGVETPRE NELGTLLGEL DTWGIQIFSI GEFSVNRPLT CVAYTIFQSR 

       850        860        870        880        890        900 
ELLTSLMIPP KTFLNFMSTL EDHYVKDNPF HNSLHAADVT QSTNVLLNTP ALEGVFTPLE 

       910        920        930        940        950        960 
VGGALFAACI HDVDHPGLTN QFLVNSSSEL ALMYNDESVL ENHHLAVAFK LLQNQGCDIF 

       970        980        990       1000       1010       1020 
CNMQKKQRQT LRKMVIDIVL STDMSKHMSL LADLKTMVET KKVAGSGVLL LDNYTDRIQV 

      1030       1040       1050       1060       1070       1080 
LENLVHCADL SNPTKPLPLY KRWVALLMEE FFLQGDKERE SGMDISPMCD RHNATIEKSQ 

      1090       1100       1110       1120       1130       1140 
VGFIDYIVHP LWETWADLVH PDAQDILDTL EENRDYYQSM IPPSPPPSGV DENPQEDRIR 

      1150       1160       1170       1180       1190       1200 
FQVTLEESDQ ENLAELEEGD ESGGESTTTG TTGTTAASAL SGAGGGGGGG GGMAPRTGGC 


QNQPQHGGM 

« Hide

Isoform II (I) (J) [UniParc].

See P12252.

Isoform III (E) [UniParc].

See P12252.

Isoform IV (A) [UniParc].

See P12252.

Isoform V (C) [UniParc].

See P12252.

Isoform VI (D) [UniParc].

See P12252.

Isoform VII (L) [UniParc].

See P12252.

Isoform F [UniParc].

See Q8IRU4.

Isoform G [UniParc].

See Q9W4S9.

Isoform M [UniParc].

See Q9W4T0.

Isoform N [UniParc].

See Q9W4S9.

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]"Characterization of the memory gene dunce of Drosophila melanogaster."
Qiu Y.H., Chen C.-N., Malone T., Richter L., Beckendorf S.K., Davis R.L.
J. Mol. Biol. 222:553-565(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 495-1209, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
Strain: Canton-S.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014298 Genomic DNA. Translation: AAF45858.3.
RefSeqNP_726846.1. NM_166959.2.
NP_726847.2. NM_166960.3.
UniGeneDm.4817.

3D structure databases

ProteinModelPortalQ9W4T4.
SMRQ9W4T4. Positions 691-725, 800-1122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid57834. 7 interactions.
IntActQ9W4T4. 1 interaction.
STRING7227.FBpp0070486.

Proteomic databases

PaxDbQ9W4T4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0070509; FBpp0070485; FBgn0000479. [Q9W4T4-1]
FBtr0333315; FBpp0305507; FBgn0000479. [Q9W4T4-1]
GeneID31309.
KEGGdme:Dmel_CG32498.
UCSCCG32498-RA. d. melanogaster. [Q9W4T4-1]

Organism-specific databases

CTD31309.
FlyBaseFBgn0000479. dnc.

Phylogenomic databases

eggNOGNOG122287.
GeneTreeENSGT00660000095451.
InParanoidQ9W4T4.
KOK01120.
OrthoDBEOG7HQNBC.
PhylomeDBQ9W4T4.

Enzyme and pathway databases

UniPathwayUPA00762; UER00747.

Gene expression databases

BgeeQ9W4T4.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSdnc. drosophila.
GenomeRNAi31309.
NextBio772953.

Entry information

Entry namePDE4A_DROME
AccessionPrimary (citable) accession number: Q9W4T4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase