Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9W4T4

- PDE4A_DROME

UniProt

Q9W4T4 - PDE4A_DROME

Protein

cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I

Gene

dnc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity. Vital for female fertility. Required for learning/memory.By similarity1 Publication

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 Publication

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei871 – 8711Proton donorBy similarity
    Metal bindingi875 – 8751Divalent metal cation 1By similarity
    Metal bindingi911 – 9111Divalent metal cation 1By similarity
    Metal bindingi912 – 9121Divalent metal cation 1By similarity
    Metal bindingi912 – 9121Divalent metal cation 2By similarity
    Binding sitei912 – 9121cAMPBy similarity
    Metal bindingi1029 – 10291Divalent metal cation 1By similarity
    Binding sitei1029 – 10291cAMPBy similarity
    Sitei1032 – 10321Binds AMP, but not cAMPBy similarity
    Binding sitei1080 – 10801cAMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi871 – 8755cAMPBy similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. associative learning Source: FlyBase
    2. axon extension Source: FlyBase
    3. behavioral response to ethanol Source: FlyBase
    4. cAMP catabolic process Source: UniProtKB-UniPathway
    5. cAMP-mediated signaling Source: FlyBase
    6. circadian rhythm Source: FlyBase
    7. conditioned taste aversion Source: FlyBase
    8. courtship behavior Source: FlyBase
    9. lateral inhibition Source: FlyBase
    10. learning Source: FlyBase
    11. learning or memory Source: FlyBase
    12. locomotor rhythm Source: FlyBase
    13. mating behavior Source: FlyBase
    14. memory Source: FlyBase
    15. nonassociative learning Source: FlyBase
    16. olfactory learning Source: FlyBase
    17. oogenesis Source: FlyBase
    18. regulation of protein kinase A signaling Source: FlyBase
    19. reproduction Source: FlyBase
    20. short-term memory Source: FlyBase
    21. synaptic transmission Source: FlyBase
    22. thermosensory behavior Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_209420. G alpha (s) signalling events.
    REACT_213958. DARPP-32 events.
    UniPathwayiUPA00762; UER00747.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I (EC:3.1.4.53)
    Alternative name(s):
    Learning/memory process protein
    Protein dunce
    Gene namesi
    Name:dncImported
    ORF Names:CG32498
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0000479. dnc.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12091209cAMP-specific 3',5'-cyclic phosphodiesterase, isoform IPRO_0000198817Add
    BLAST

    Proteomic databases

    PaxDbiQ9W4T4.

    Expressioni

    Gene expression databases

    BgeeiQ9W4T4.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi57834. 7 interactions.
    IntActiQ9W4T4. 1 interaction.
    STRINGi7227.FBpp0070486.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9W4T4.
    SMRiQ9W4T4. Positions 691-725, 800-1122.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 10483Gly-richAdd
    BLAST
    Compositional biasi242 – 32180Gln-richAdd
    BLAST
    Compositional biasi1159 – 120850Gly-richAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG122287.
    GeneTreeiENSGT00660000095451.
    InParanoidiQ9W4T4.
    KOiK01120.
    OMAiINEEMET.
    OrthoDBiEOG7HQNBC.
    PhylomeDBiQ9W4T4.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequences (12)i

    Sequence statusi: Complete.

    This entry describes 12 isoformsi produced by alternative splicing. Align

    Isoform I1 Publication (identifier: Q9W4T4-1) [UniParc]FASTAAdd to Basket

    Also known as: B1 Publication

    , S

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMRLARFTQG FSFNKNVAKH RGSGSSNGTG NGSGTGNGQG LGGSCSANGL     50
    AATGGVGGGG GSGSGGGGCG SGSGSGSGKR KSKARTKCFG GTVFRCCLPC 100
    RGGGSAAPAT SPPQTPAQTP DELKVIPEDC NLEKSAEQKR DLLERNNKED 150
    DLLNRTVSGS ELDLYGGAGG GTKKHSLADT IDTSVTTPIS LKTLINDVDE 200
    ELDQQLSAAD IAAASLASGL VARRAEPETL SDASVSPTAV VQQQQQQQQQ 250
    LQQPLLQSQP HFVPSSGNIL SQVTLYSGSN PSTNPCQSAV QNQGQNSNPN 300
    PNQNPNTNPN QNQQRCSCQP QTSPLPHIKE EEESDQANFK HQTSLKEHQP 350
    LPPPITIATG YCGSCESVHH SSATSSSAGT VPPGGQQTQE YIAGTSSTPS 400
    PRIKLKFRKP HKSCWSRIVL APIGSAGGSS SATTVIGSNS NETLASSSTT 450
    GGTATTTQNS SSVSVAAHHR LTSSSASALA TSHPSNSQLL PTSKMQAEQG 500
    SIGDLQKYHS RYLKNRRHTL ANVRFDVENG QGARSPLEGG SPSAGLVLQN 550
    LPQRRESFLY RSDSDFEMSP KSMSRNSSIA SERFKEQEAS ILVDRSHGED 600
    LIVTPFAQIL ASLRSVRNNL LSLTNVPASN KSRRPNQSSS ASRSGNPPGA 650
    PLSQGEEAYT RLATDTIEEL DWCLDQLETI QTHRSVSDMA SLKFKRMLNK 700
    ELSHFSESSR SGNQISEYIC STFLDKQQEF DLPSLRVEDN PELVAANAAA 750
    GQQSAGQYAR SRSPRGPPMS QISGVKRPLS HTNSFTGERL PTFGVETPRE 800
    NELGTLLGEL DTWGIQIFSI GEFSVNRPLT CVAYTIFQSR ELLTSLMIPP 850
    KTFLNFMSTL EDHYVKDNPF HNSLHAADVT QSTNVLLNTP ALEGVFTPLE 900
    VGGALFAACI HDVDHPGLTN QFLVNSSSEL ALMYNDESVL ENHHLAVAFK 950
    LLQNQGCDIF CNMQKKQRQT LRKMVIDIVL STDMSKHMSL LADLKTMVET 1000
    KKVAGSGVLL LDNYTDRIQV LENLVHCADL SNPTKPLPLY KRWVALLMEE 1050
    FFLQGDKERE SGMDISPMCD RHNATIEKSQ VGFIDYIVHP LWETWADLVH 1100
    PDAQDILDTL EENRDYYQSM IPPSPPPSGV DENPQEDRIR FQVTLEESDQ 1150
    ENLAELEEGD ESGGESTTTG TTGTTAASAL SGAGGGGGGG GGMAPRTGGC 1200
    QNQPQHGGM 1209
    Length:1,209
    Mass (Da):129,413
    Last modified:July 5, 2004 - v2
    Checksum:iA7E00D5BA862C1BE
    GO
    Isoform II1 Publication (identifier: P12252-1) [UniParc]FASTAAdd to Basket

    Also known as: I1 Publication

    , J1 Publication

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,070
    Mass (Da):115,079
    GO
    Isoform III (identifier: P12252-7) [UniParc]FASTAAdd to Basket

    Also known as: E, P

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative initiation at Met-429 of isoform II.

    Length:642
    Mass (Da):70,625
    GO
    Isoform IV1 Publication (identifier: P12252-3) [UniParc]FASTAAdd to Basket

    Also known as: A1 Publication

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:701
    Mass (Da):77,193
    GO
    Isoform V1 Publication (identifier: P12252-4) [UniParc]FASTAAdd to Basket

    Also known as: C1 Publication

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,057
    Mass (Da):113,506
    GO
    Isoform VI1 Publication (identifier: P12252-5) [UniParc]FASTAAdd to Basket

    Also known as: D1 Publication

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,068
    Mass (Da):114,836
    GO
    Isoform VII1 Publication (identifier: P12252-6) [UniParc]FASTAAdd to Basket

    Also known as: L1 Publication

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:521
    Mass (Da):57,358
    GO
    Isoform F1 Publication (identifier: Q8IRU4-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q8IRU4.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.Curated

    Length:662
    Mass (Da):72,954
    GO
    Isoform G1 Publication (identifier: Q9W4S9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q9W4S9.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.Curated

    Length:814
    Mass (Da):89,085
    GO
    Isoform N1 Publication (identifier: Q9W4S9-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q9W4S9.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.Curated

    Length:983
    Mass (Da):107,341
    GO
    Isoform R (identifier: P12252-8) [UniParc] [UniParc]FASTAAdd to Basket

    Also known as: Q

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.

    Length:703
    Mass (Da):77,368
    GO
    Isoform U (identifier: P12252-9) [UniParc]FASTAAdd to Basket

    Also known as: T

    The sequence of this isoform can be found in the external entry P12252.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.

    Length:715
    Mass (Da):78,887
    GO

    Sequence cautioni

    The sequence CAA19668.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1097 – 10971D → S no nucleotide entry (PubMed:1660926)Curated
    Sequence conflicti1166 – 11661S → T no nucleotide entry (PubMed:1660926)Curated
    Sequence conflicti1181 – 11822SG → R no nucleotide entry (PubMed:1660926)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF45858.3.
    AL024484 Genomic DNA. Translation: CAA19668.1. Sequence problems.
    RefSeqiNP_726846.1. NM_166959.2. [Q9W4T4-1]
    NP_726847.2. NM_166960.3. [Q9W4T4-1]
    UniGeneiDm.4817.

    Genome annotation databases

    EnsemblMetazoaiFBtr0070509; FBpp0070485; FBgn0000479. [Q9W4T4-1]
    FBtr0333315; FBpp0305507; FBgn0000479. [Q9W4T4-1]
    GeneIDi31309.
    KEGGidme:Dmel_CG32498.
    UCSCiCG32498-RA. d. melanogaster. [Q9W4T4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF45858.3 .
    AL024484 Genomic DNA. Translation: CAA19668.1 . Sequence problems.
    RefSeqi NP_726846.1. NM_166959.2. [Q9W4T4-1 ]
    NP_726847.2. NM_166960.3. [Q9W4T4-1 ]
    UniGenei Dm.4817.

    3D structure databases

    ProteinModelPortali Q9W4T4.
    SMRi Q9W4T4. Positions 691-725, 800-1122.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 57834. 7 interactions.
    IntActi Q9W4T4. 1 interaction.
    STRINGi 7227.FBpp0070486.

    Proteomic databases

    PaxDbi Q9W4T4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0070509 ; FBpp0070485 ; FBgn0000479 . [Q9W4T4-1 ]
    FBtr0333315 ; FBpp0305507 ; FBgn0000479 . [Q9W4T4-1 ]
    GeneIDi 31309.
    KEGGi dme:Dmel_CG32498.
    UCSCi CG32498-RA. d. melanogaster. [Q9W4T4-1 ]

    Organism-specific databases

    CTDi 31309.
    FlyBasei FBgn0000479. dnc.

    Phylogenomic databases

    eggNOGi NOG122287.
    GeneTreei ENSGT00660000095451.
    InParanoidi Q9W4T4.
    KOi K01120.
    OMAi INEEMET.
    OrthoDBi EOG7HQNBC.
    PhylomeDBi Q9W4T4.

    Enzyme and pathway databases

    UniPathwayi UPA00762 ; UER00747 .
    Reactomei REACT_209420. G alpha (s) signalling events.
    REACT_213958. DARPP-32 events.

    Miscellaneous databases

    ChiTaRSi dnc. drosophila.
    GenomeRNAii 31309.
    NextBioi 772953.

    Gene expression databases

    Bgeei Q9W4T4.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-524.
      Strain: Oregon-R.
    4. "Characterization of the memory gene dunce of Drosophila melanogaster."
      Qiu Y.H., Chen C.-N., Malone T., Richter L., Beckendorf S.K., Davis R.L.
      J. Mol. Biol. 222:553-565(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 495-1209, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
      Strain: Canton-S1 Publication.

    Entry informationi

    Entry nameiPDE4A_DROME
    AccessioniPrimary (citable) accession number: Q9W4T4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3