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Q9W4T4

- PDE4A_DROME

UniProt

Q9W4T4 - PDE4A_DROME

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Protein
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I
Gene
dnc, CG32498
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity. Vital for female fertility. Required for learning/memory.1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 Publication

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei871 – 8711Proton donor By similarity
Metal bindingi875 – 8751Divalent metal cation 1 By similarity
Metal bindingi911 – 9111Divalent metal cation 1 By similarity
Metal bindingi912 – 9121Divalent metal cation 1 By similarity
Metal bindingi912 – 9121Divalent metal cation 2 By similarity
Binding sitei912 – 9121cAMP By similarity
Metal bindingi1029 – 10291Divalent metal cation 1 By similarity
Binding sitei1029 – 10291cAMP By similarity
Sitei1032 – 10321Binds AMP, but not cAMP By similarity
Binding sitei1080 – 10801cAMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi871 – 8755cAMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. associative learning Source: FlyBase
  2. axon extension Source: FlyBase
  3. behavioral response to ethanol Source: FlyBase
  4. cAMP catabolic process Source: UniProtKB-UniPathway
  5. cAMP-mediated signaling Source: FlyBase
  6. circadian rhythm Source: FlyBase
  7. conditioned taste aversion Source: FlyBase
  8. courtship behavior Source: FlyBase
  9. lateral inhibition Source: FlyBase
  10. learning Source: FlyBase
  11. learning or memory Source: FlyBase
  12. locomotor rhythm Source: FlyBase
  13. mating behavior Source: FlyBase
  14. memory Source: FlyBase
  15. nonassociative learning Source: FlyBase
  16. olfactory learning Source: FlyBase
  17. oogenesis Source: FlyBase
  18. regulation of protein kinase A signaling Source: FlyBase
  19. reproduction Source: FlyBase
  20. short-term memory Source: FlyBase
  21. synaptic transmission Source: FlyBase
  22. thermosensory behavior Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I (EC:3.1.4.53)
Alternative name(s):
Learning/memory process protein
Protein dunce
Gene namesi
Name:dnc
ORF Names:CG32498
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000479. dnc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12091209cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I
PRO_0000198817Add
BLAST

Proteomic databases

PaxDbiQ9W4T4.

Expressioni

Gene expression databases

BgeeiQ9W4T4.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi57834. 7 interactions.
IntActiQ9W4T4. 1 interaction.
STRINGi7227.FBpp0070486.

Structurei

3D structure databases

ProteinModelPortaliQ9W4T4.
SMRiQ9W4T4. Positions 691-725, 800-1122.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 10483Gly-rich
Add
BLAST
Compositional biasi242 – 32180Gln-rich
Add
BLAST
Compositional biasi1159 – 120850Gly-rich
Add
BLAST

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.1 Publication

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00660000095451.
InParanoidiQ9W4T4.
KOiK01120.
OMAiINEEMET.
OrthoDBiEOG7HQNBC.
PhylomeDBiQ9W4T4.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. Align

Isoform I (identifier: Q9W4T4-1) [UniParc]FASTAAdd to Basket

Also known as: B, S

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMRLARFTQG FSFNKNVAKH RGSGSSNGTG NGSGTGNGQG LGGSCSANGL     50
AATGGVGGGG GSGSGGGGCG SGSGSGSGKR KSKARTKCFG GTVFRCCLPC 100
RGGGSAAPAT SPPQTPAQTP DELKVIPEDC NLEKSAEQKR DLLERNNKED 150
DLLNRTVSGS ELDLYGGAGG GTKKHSLADT IDTSVTTPIS LKTLINDVDE 200
ELDQQLSAAD IAAASLASGL VARRAEPETL SDASVSPTAV VQQQQQQQQQ 250
LQQPLLQSQP HFVPSSGNIL SQVTLYSGSN PSTNPCQSAV QNQGQNSNPN 300
PNQNPNTNPN QNQQRCSCQP QTSPLPHIKE EEESDQANFK HQTSLKEHQP 350
LPPPITIATG YCGSCESVHH SSATSSSAGT VPPGGQQTQE YIAGTSSTPS 400
PRIKLKFRKP HKSCWSRIVL APIGSAGGSS SATTVIGSNS NETLASSSTT 450
GGTATTTQNS SSVSVAAHHR LTSSSASALA TSHPSNSQLL PTSKMQAEQG 500
SIGDLQKYHS RYLKNRRHTL ANVRFDVENG QGARSPLEGG SPSAGLVLQN 550
LPQRRESFLY RSDSDFEMSP KSMSRNSSIA SERFKEQEAS ILVDRSHGED 600
LIVTPFAQIL ASLRSVRNNL LSLTNVPASN KSRRPNQSSS ASRSGNPPGA 650
PLSQGEEAYT RLATDTIEEL DWCLDQLETI QTHRSVSDMA SLKFKRMLNK 700
ELSHFSESSR SGNQISEYIC STFLDKQQEF DLPSLRVEDN PELVAANAAA 750
GQQSAGQYAR SRSPRGPPMS QISGVKRPLS HTNSFTGERL PTFGVETPRE 800
NELGTLLGEL DTWGIQIFSI GEFSVNRPLT CVAYTIFQSR ELLTSLMIPP 850
KTFLNFMSTL EDHYVKDNPF HNSLHAADVT QSTNVLLNTP ALEGVFTPLE 900
VGGALFAACI HDVDHPGLTN QFLVNSSSEL ALMYNDESVL ENHHLAVAFK 950
LLQNQGCDIF CNMQKKQRQT LRKMVIDIVL STDMSKHMSL LADLKTMVET 1000
KKVAGSGVLL LDNYTDRIQV LENLVHCADL SNPTKPLPLY KRWVALLMEE 1050
FFLQGDKERE SGMDISPMCD RHNATIEKSQ VGFIDYIVHP LWETWADLVH 1100
PDAQDILDTL EENRDYYQSM IPPSPPPSGV DENPQEDRIR FQVTLEESDQ 1150
ENLAELEEGD ESGGESTTTG TTGTTAASAL SGAGGGGGGG GGMAPRTGGC 1200
QNQPQHGGM 1209
Length:1,209
Mass (Da):129,413
Last modified:July 5, 2004 - v2
Checksum:iA7E00D5BA862C1BE
GO
Isoform II1 Publication (identifier: P12252-1) [UniParc]FASTAAdd to Basket

Also known as: I, J

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,070
Mass (Da):115,079
GO
Isoform III (identifier: P12252-7) [UniParc]FASTAAdd to Basket

Also known as: E, P

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-429 of isoform II.

Length:642
Mass (Da):70,625
GO
Isoform IV1 Publication (identifier: P12252-3) [UniParc]FASTAAdd to Basket

Also known as: A

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:701
Mass (Da):77,193
GO
Isoform V1 Publication (identifier: P12252-4) [UniParc]FASTAAdd to Basket

Also known as: C

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,057
Mass (Da):113,506
GO
Isoform VI1 Publication (identifier: P12252-5) [UniParc]FASTAAdd to Basket

Also known as: D

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,068
Mass (Da):114,836
GO
Isoform VII1 Publication (identifier: P12252-6) [UniParc]FASTAAdd to Basket

Also known as: L

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:521
Mass (Da):57,358
GO
Isoform F1 Publication (identifier: Q8IRU4-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q8IRU4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:662
Mass (Da):72,954
GO
Isoform G1 Publication (identifier: Q9W4S9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:814
Mass (Da):89,085
GO
Isoform N1 Publication (identifier: Q9W4S9-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:983
Mass (Da):107,341
GO
Isoform R (identifier: P12252-8) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: Q

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:703
Mass (Da):77,368
GO
Isoform U (identifier: P12252-9) [UniParc]FASTAAdd to Basket

Also known as: T

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:715
Mass (Da):78,887
GO

Sequence cautioni

The sequence CAA19668.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1097 – 10971D → S no nucleotide entry 1 Publication
Sequence conflicti1166 – 11661S → T no nucleotide entry 1 Publication
Sequence conflicti1181 – 11822SG → R no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45858.3.
AL024484 Genomic DNA. Translation: CAA19668.1. Sequence problems.
RefSeqiNP_726846.1. NM_166959.2. [Q9W4T4-1]
NP_726847.2. NM_166960.3. [Q9W4T4-1]
UniGeneiDm.4817.

Genome annotation databases

EnsemblMetazoaiFBtr0070509; FBpp0070485; FBgn0000479. [Q9W4T4-1]
FBtr0333315; FBpp0305507; FBgn0000479. [Q9W4T4-1]
GeneIDi31309.
KEGGidme:Dmel_CG32498.
UCSCiCG32498-RA. d. melanogaster. [Q9W4T4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45858.3 .
AL024484 Genomic DNA. Translation: CAA19668.1 . Sequence problems.
RefSeqi NP_726846.1. NM_166959.2. [Q9W4T4-1 ]
NP_726847.2. NM_166960.3. [Q9W4T4-1 ]
UniGenei Dm.4817.

3D structure databases

ProteinModelPortali Q9W4T4.
SMRi Q9W4T4. Positions 691-725, 800-1122.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 57834. 7 interactions.
IntActi Q9W4T4. 1 interaction.
STRINGi 7227.FBpp0070486.

Proteomic databases

PaxDbi Q9W4T4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0070509 ; FBpp0070485 ; FBgn0000479 . [Q9W4T4-1 ]
FBtr0333315 ; FBpp0305507 ; FBgn0000479 . [Q9W4T4-1 ]
GeneIDi 31309.
KEGGi dme:Dmel_CG32498.
UCSCi CG32498-RA. d. melanogaster. [Q9W4T4-1 ]

Organism-specific databases

CTDi 31309.
FlyBasei FBgn0000479. dnc.

Phylogenomic databases

eggNOGi NOG122287.
GeneTreei ENSGT00660000095451.
InParanoidi Q9W4T4.
KOi K01120.
OMAi INEEMET.
OrthoDBi EOG7HQNBC.
PhylomeDBi Q9W4T4.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .
Reactomei REACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.

Miscellaneous databases

ChiTaRSi dnc. drosophila.
GenomeRNAii 31309.
NextBioi 772953.

Gene expression databases

Bgeei Q9W4T4.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-524.
    Strain: Oregon-R.
  4. "Characterization of the memory gene dunce of Drosophila melanogaster."
    Qiu Y.H., Chen C.-N., Malone T., Richter L., Beckendorf S.K., Davis R.L.
    J. Mol. Biol. 222:553-565(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 495-1209, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: Canton-S.

Entry informationi

Entry nameiPDE4A_DROME
AccessioniPrimary (citable) accession number: Q9W4T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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