Q9W4T4 (PDE4A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I
EC=3.1.4.17

Alternative name(s):
Learning/memory process protein
Protein dunce

Gene names

Name:	dnc
ORF Names:	CG32498

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity. Vital for female fertility. Required for learning/memory. Ref.3
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. Ref.3
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Monomer. Ref.3
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Ref.3

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon extension Inferred from mutant phenotype PubMed 14960616. Source: FlyBase behavioral response to ethanol Non-traceable author statement PubMed 11715043. Source: FlyBase cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway cAMP-mediated signaling Non-traceable author statement PubMed 11092827PubMed 11462211PubMed 11741030PubMed 14515157. Source: FlyBase conditioned taste aversion Inferred from mutant phenotype PubMed 19158309. Source: FlyBase courtship behavior Traceable author statement PubMed 11092827PubMed 11715043PubMed 12486700. Source: FlyBase lateral inhibition Inferred from mutant phenotype PubMed 19363474. Source: FlyBase locomotor rhythm Non-traceable author statement PubMed 11715043. Source: FlyBase nonassociative learning Traceable author statement PubMed 14504657. Source: FlyBase olfactory learning Traceable author statement PubMed 11462211PubMed 11715043. Source: FlyBase oogenesis Traceable author statement PubMed 3089870. Source: FlyBase short-term memory Inferred from mutant phenotype PubMed 19158309. Source: FlyBase synaptic transmission Inferred from mutant phenotype PubMed 12040051PubMed 14507965. Source: FlyBase
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 11 isoforms produced by alternative splicing. [Align] [Select]

Isoform I Ref.3 (identifier: Q9W4T4-1)

Also known as: B; O;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II Ref.3 (identifier: P12252-1)

Also known as: I; J;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform III (identifier: P12252-7)

Also known as: E;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-429 of isoform II.

Isoform IV Ref.3 (identifier: P12252-3)

Also known as: A;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform V Ref.3 (identifier: P12252-4)

Also known as: C;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VI Ref.3 (identifier: P12252-5)

Also known as: D;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VII Ref.3 (identifier: P12252-6)

Also known as: L;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform F Ref.1 (identifier: Q8IRU4-1)

The sequence of this isoform can be found in the external entry Q8IRU4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform G Ref.1 (identifier: Q9W4S9-2)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform M Ref.1 (identifier: Q9W4T0-1)

The sequence of this isoform can be found in the external entry Q9W4T0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform N Ref.1 (identifier: Q9W4S9-1)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1209	1209	cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I		PRO_0000198817
Regions
	Nucleotide binding	871 – 875	5	cAMP By similarity
Sites
	Active site	871	1	Proton donor By similarity
	Metal binding	875	1	Divalent metal cation 1 By similarity
	Metal binding	911	1	Divalent metal cation 1 By similarity
	Metal binding	912	1	Divalent metal cation 1 By similarity
	Metal binding	912	1	Divalent metal cation 2 By similarity
	Metal binding	1029	1	Divalent metal cation 1 By similarity
	Binding site	912	1	cAMP By similarity
	Binding site	1029	1	cAMP By similarity
	Binding site	1080	1	cAMP By similarity
	Site	1032	1	Binds AMP, but not cAMP By similarity
Experimental info
	Sequence conflict	1097	1	D → S Ref.3
	Sequence conflict	1166	1	S → T Ref.3
	Sequence conflict	1181 – 1182	2	SG → R Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform I (B) (O) [UniParc]. Last modified July 5, 2004. Version 2. Checksum: A7E00D5BA862C1BE	FASTA	1,209	129,413
	10 20 30 40 50 60 MMRLARFTQG FSFNKNVAKH RGSGSSNGTG NGSGTGNGQG LGGSCSANGL AATGGVGGGG 70 80 90 100 110 120 GSGSGGGGCG SGSGSGSGKR KSKARTKCFG GTVFRCCLPC RGGGSAAPAT SPPQTPAQTP 130 140 150 160 170 180 DELKVIPEDC NLEKSAEQKR DLLERNNKED DLLNRTVSGS ELDLYGGAGG GTKKHSLADT 190 200 210 220 230 240 IDTSVTTPIS LKTLINDVDE ELDQQLSAAD IAAASLASGL VARRAEPETL SDASVSPTAV 250 260 270 280 290 300 VQQQQQQQQQ LQQPLLQSQP HFVPSSGNIL SQVTLYSGSN PSTNPCQSAV QNQGQNSNPN 310 320 330 340 350 360 PNQNPNTNPN QNQQRCSCQP QTSPLPHIKE EEESDQANFK HQTSLKEHQP LPPPITIATG 370 380 390 400 410 420 YCGSCESVHH SSATSSSAGT VPPGGQQTQE YIAGTSSTPS PRIKLKFRKP HKSCWSRIVL 430 440 450 460 470 480 APIGSAGGSS SATTVIGSNS NETLASSSTT GGTATTTQNS SSVSVAAHHR LTSSSASALA 490 500 510 520 530 540 TSHPSNSQLL PTSKMQAEQG SIGDLQKYHS RYLKNRRHTL ANVRFDVENG QGARSPLEGG 550 560 570 580 590 600 SPSAGLVLQN LPQRRESFLY RSDSDFEMSP KSMSRNSSIA SERFKEQEAS ILVDRSHGED 610 620 630 640 650 660 LIVTPFAQIL ASLRSVRNNL LSLTNVPASN KSRRPNQSSS ASRSGNPPGA PLSQGEEAYT 670 680 690 700 710 720 RLATDTIEEL DWCLDQLETI QTHRSVSDMA SLKFKRMLNK ELSHFSESSR SGNQISEYIC 730 740 750 760 770 780 STFLDKQQEF DLPSLRVEDN PELVAANAAA GQQSAGQYAR SRSPRGPPMS QISGVKRPLS 790 800 810 820 830 840 HTNSFTGERL PTFGVETPRE NELGTLLGEL DTWGIQIFSI GEFSVNRPLT CVAYTIFQSR 850 860 870 880 890 900 ELLTSLMIPP KTFLNFMSTL EDHYVKDNPF HNSLHAADVT QSTNVLLNTP ALEGVFTPLE 910 920 930 940 950 960 VGGALFAACI HDVDHPGLTN QFLVNSSSEL ALMYNDESVL ENHHLAVAFK LLQNQGCDIF 970 980 990 1000 1010 1020 CNMQKKQRQT LRKMVIDIVL STDMSKHMSL LADLKTMVET KKVAGSGVLL LDNYTDRIQV 1030 1040 1050 1060 1070 1080 LENLVHCADL SNPTKPLPLY KRWVALLMEE FFLQGDKERE SGMDISPMCD RHNATIEKSQ 1090 1100 1110 1120 1130 1140 VGFIDYIVHP LWETWADLVH PDAQDILDTL EENRDYYQSM IPPSPPPSGV DENPQEDRIR 1150 1160 1170 1180 1190 1200 FQVTLEESDQ ENLAELEEGD ESGGESTTTG TTGTTAASAL SGAGGGGGGG GGMAPRTGGC QNQPQHGGM
	Isoform II (I) (J) [UniParc]. See P12252.		–	–
	Isoform III (E) [UniParc]. See P12252.		–	–
	Isoform IV (A) [UniParc]. See P12252.		–	–
	Isoform V (C) [UniParc]. See P12252.		–	–
	Isoform VI (D) [UniParc]. See P12252.		–	–
	Isoform VII (L) [UniParc]. See P12252.		–	–
	Isoform F [UniParc]. See Q8IRU4.		–	–
	Isoform G [UniParc]. See Q9W4S9.		–	–
	Isoform M [UniParc]. See Q9W4T0.		–	–
	Isoform N [UniParc]. See Q9W4S9.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"Characterization of the memory gene dunce of Drosophila melanogaster." Qiu Y.H., Chen C.-N., Malone T., Richter L., Beckendorf S.K., Davis R.L. J. Mol. Biol. 222:553-565(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 495-1209, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT. Strain: Canton-S.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF45858.3.
RefSeq	NP_726846.1. NM_166959.2. NP_726847.2. NM_166960.3.
UniGene	Dm.4817.
3D structure databases
ProteinModelPortal	Q9W4T4.
SMR	Q9W4T4. Positions 691-725, 800-1121.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9W4T4. 1 interaction.
STRING	7227.FBpp0070486.
Proteomic databases
PaxDb	Q9W4T4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0070509; FBpp0070485; FBgn0000479. FBtr0333315; FBpp0305507; FBgn0000479.
GeneID	31309.
KEGG	dme:Dmel_CG32498.
UCSC	CG32498-RA. d. melanogaster.
Organism-specific databases
CTD	31309.
FlyBase	FBgn0000479. dnc.
Phylogenomic databases
eggNOG	NOG122287.
GeneTree	ENSGT00690000101692.
InParanoid	Q9W4T4.
KO	K01120.
OrthoDB	EOG44MW75.
PhylomeDB	Q9W4T4.
Enzyme and pathway databases
UniPathway	UPA00762; UER00747.
Gene expression databases
Bgee	Q9W4T4.
GermOnline	CG32498. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	dnc. drosophila.
GenomeRNAi	31309.
NextBio	772953.

Entry information

Entry name

PDE4A_DROME

Accession

Primary (citable) accession number: Q9W4T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project