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Q9W4T4

- PDE4A_DROME

UniProt

Q9W4T4 - PDE4A_DROME

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Protein

cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I

Gene

dnc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Vital for female fertility. Required for learning/memory.By similarity1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 Publication

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei871 – 8711Proton donorBy similarity
Metal bindingi875 – 8751Divalent metal cation 1By similarity
Metal bindingi911 – 9111Divalent metal cation 1By similarity
Metal bindingi912 – 9121Divalent metal cation 1By similarity
Metal bindingi912 – 9121Divalent metal cation 2By similarity
Binding sitei912 – 9121cAMPBy similarity
Metal bindingi1029 – 10291Divalent metal cation 1By similarity
Binding sitei1029 – 10291cAMPBy similarity
Sitei1032 – 10321Binds AMP, but not cAMPBy similarity
Binding sitei1080 – 10801cAMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi871 – 8755cAMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. associative learning Source: FlyBase
  2. axon extension Source: FlyBase
  3. behavioral response to ethanol Source: FlyBase
  4. cAMP catabolic process Source: UniProtKB-UniPathway
  5. cAMP-mediated signaling Source: FlyBase
  6. circadian rhythm Source: FlyBase
  7. conditioned taste aversion Source: FlyBase
  8. courtship behavior Source: FlyBase
  9. lateral inhibition Source: FlyBase
  10. learning Source: FlyBase
  11. learning or memory Source: FlyBase
  12. locomotor rhythm Source: FlyBase
  13. mating behavior Source: FlyBase
  14. memory Source: FlyBase
  15. nonassociative learning Source: FlyBase
  16. olfactory learning Source: FlyBase
  17. oogenesis Source: FlyBase
  18. regulation of protein kinase A signaling Source: FlyBase
  19. reproduction Source: FlyBase
  20. short-term memory Source: FlyBase
  21. synaptic transmission Source: FlyBase
  22. thermosensory behavior Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform I (EC:3.1.4.53)
Alternative name(s):
Learning/memory process protein
Protein dunce
Gene namesi
Name:dncImported
ORF Names:CG32498
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000479. dnc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12091209cAMP-specific 3',5'-cyclic phosphodiesterase, isoform IPRO_0000198817Add
BLAST

Proteomic databases

PaxDbiQ9W4T4.

Expressioni

Gene expression databases

BgeeiQ9W4T4.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi57834. 7 interactions.
IntActiQ9W4T4. 1 interaction.
STRINGi7227.FBpp0070486.

Structurei

3D structure databases

ProteinModelPortaliQ9W4T4.
SMRiQ9W4T4. Positions 691-725, 800-1122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 10483Gly-richAdd
BLAST
Compositional biasi242 – 32180Gln-richAdd
BLAST
Compositional biasi1159 – 120850Gly-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00760000118889.
InParanoidiQ9W4T4.
KOiK13293.
OMAiINEEMET.
OrthoDBiEOG7HQNBC.
PhylomeDBiQ9W4T4.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. Align

Isoform I1 Publication (identifier: Q9W4T4-1) [UniParc]FASTAAdd to Basket

Also known as: B1 Publication

, S

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRLARFTQG FSFNKNVAKH RGSGSSNGTG NGSGTGNGQG LGGSCSANGL
60 70 80 90 100
AATGGVGGGG GSGSGGGGCG SGSGSGSGKR KSKARTKCFG GTVFRCCLPC
110 120 130 140 150
RGGGSAAPAT SPPQTPAQTP DELKVIPEDC NLEKSAEQKR DLLERNNKED
160 170 180 190 200
DLLNRTVSGS ELDLYGGAGG GTKKHSLADT IDTSVTTPIS LKTLINDVDE
210 220 230 240 250
ELDQQLSAAD IAAASLASGL VARRAEPETL SDASVSPTAV VQQQQQQQQQ
260 270 280 290 300
LQQPLLQSQP HFVPSSGNIL SQVTLYSGSN PSTNPCQSAV QNQGQNSNPN
310 320 330 340 350
PNQNPNTNPN QNQQRCSCQP QTSPLPHIKE EEESDQANFK HQTSLKEHQP
360 370 380 390 400
LPPPITIATG YCGSCESVHH SSATSSSAGT VPPGGQQTQE YIAGTSSTPS
410 420 430 440 450
PRIKLKFRKP HKSCWSRIVL APIGSAGGSS SATTVIGSNS NETLASSSTT
460 470 480 490 500
GGTATTTQNS SSVSVAAHHR LTSSSASALA TSHPSNSQLL PTSKMQAEQG
510 520 530 540 550
SIGDLQKYHS RYLKNRRHTL ANVRFDVENG QGARSPLEGG SPSAGLVLQN
560 570 580 590 600
LPQRRESFLY RSDSDFEMSP KSMSRNSSIA SERFKEQEAS ILVDRSHGED
610 620 630 640 650
LIVTPFAQIL ASLRSVRNNL LSLTNVPASN KSRRPNQSSS ASRSGNPPGA
660 670 680 690 700
PLSQGEEAYT RLATDTIEEL DWCLDQLETI QTHRSVSDMA SLKFKRMLNK
710 720 730 740 750
ELSHFSESSR SGNQISEYIC STFLDKQQEF DLPSLRVEDN PELVAANAAA
760 770 780 790 800
GQQSAGQYAR SRSPRGPPMS QISGVKRPLS HTNSFTGERL PTFGVETPRE
810 820 830 840 850
NELGTLLGEL DTWGIQIFSI GEFSVNRPLT CVAYTIFQSR ELLTSLMIPP
860 870 880 890 900
KTFLNFMSTL EDHYVKDNPF HNSLHAADVT QSTNVLLNTP ALEGVFTPLE
910 920 930 940 950
VGGALFAACI HDVDHPGLTN QFLVNSSSEL ALMYNDESVL ENHHLAVAFK
960 970 980 990 1000
LLQNQGCDIF CNMQKKQRQT LRKMVIDIVL STDMSKHMSL LADLKTMVET
1010 1020 1030 1040 1050
KKVAGSGVLL LDNYTDRIQV LENLVHCADL SNPTKPLPLY KRWVALLMEE
1060 1070 1080 1090 1100
FFLQGDKERE SGMDISPMCD RHNATIEKSQ VGFIDYIVHP LWETWADLVH
1110 1120 1130 1140 1150
PDAQDILDTL EENRDYYQSM IPPSPPPSGV DENPQEDRIR FQVTLEESDQ
1160 1170 1180 1190 1200
ENLAELEEGD ESGGESTTTG TTGTTAASAL SGAGGGGGGG GGMAPRTGGC

QNQPQHGGM
Length:1,209
Mass (Da):129,413
Last modified:July 5, 2004 - v2
Checksum:iA7E00D5BA862C1BE
GO
Isoform II1 Publication (identifier: P12252-1) [UniParc]FASTAAdd to Basket

Also known as: I1 Publication

, J1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,070
Mass (Da):115,079
GO
Isoform III (identifier: P12252-7) [UniParc]FASTAAdd to Basket

Also known as: E, P

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-429 of isoform II.

Length:642
Mass (Da):70,625
GO
Isoform IV1 Publication (identifier: P12252-3) [UniParc]FASTAAdd to Basket

Also known as: A1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:701
Mass (Da):77,193
GO
Isoform V1 Publication (identifier: P12252-4) [UniParc]FASTAAdd to Basket

Also known as: C1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,057
Mass (Da):113,506
GO
Isoform VI1 Publication (identifier: P12252-5) [UniParc]FASTAAdd to Basket

Also known as: D1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,068
Mass (Da):114,836
GO
Isoform VII1 Publication (identifier: P12252-6) [UniParc]FASTAAdd to Basket

Also known as: L1 Publication

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:521
Mass (Da):57,358
GO
Isoform F1 Publication (identifier: Q8IRU4-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q8IRU4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.Curated

Length:662
Mass (Da):72,954
GO
Isoform G1 Publication (identifier: Q9W4S9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.Curated

Length:814
Mass (Da):89,085
GO
Isoform N1 Publication (identifier: Q9W4S9-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.Curated

Length:983
Mass (Da):107,341
GO
Isoform R (identifier: P12252-8) [UniParc] [UniParc]FASTAAdd to Basket

Also known as: Q

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:703
Mass (Da):77,368
GO
Isoform U (identifier: P12252-9) [UniParc]FASTAAdd to Basket

Also known as: T

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:715
Mass (Da):78,887
GO

Sequence cautioni

The sequence CAA19668.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1097 – 10971D → S no nucleotide entry (PubMed:1660926)Curated
Sequence conflicti1166 – 11661S → T no nucleotide entry (PubMed:1660926)Curated
Sequence conflicti1181 – 11822SG → R no nucleotide entry (PubMed:1660926)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45858.3.
AL024484 Genomic DNA. Translation: CAA19668.1. Sequence problems.
RefSeqiNP_726846.1. NM_166959.2. [Q9W4T4-1]
NP_726847.2. NM_166960.3. [Q9W4T4-1]
UniGeneiDm.4817.

Genome annotation databases

EnsemblMetazoaiFBtr0070509; FBpp0070485; FBgn0000479. [Q9W4T4-1]
FBtr0333315; FBpp0305507; FBgn0000479. [Q9W4T4-1]
GeneIDi31309.
KEGGidme:Dmel_CG32498.
UCSCiCG32498-RA. d. melanogaster. [Q9W4T4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014298 Genomic DNA. Translation: AAF45858.3 .
AL024484 Genomic DNA. Translation: CAA19668.1 . Sequence problems.
RefSeqi NP_726846.1. NM_166959.2. [Q9W4T4-1 ]
NP_726847.2. NM_166960.3. [Q9W4T4-1 ]
UniGenei Dm.4817.

3D structure databases

ProteinModelPortali Q9W4T4.
SMRi Q9W4T4. Positions 691-725, 800-1122.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 57834. 7 interactions.
IntActi Q9W4T4. 1 interaction.
STRINGi 7227.FBpp0070486.

Proteomic databases

PaxDbi Q9W4T4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0070509 ; FBpp0070485 ; FBgn0000479 . [Q9W4T4-1 ]
FBtr0333315 ; FBpp0305507 ; FBgn0000479 . [Q9W4T4-1 ]
GeneIDi 31309.
KEGGi dme:Dmel_CG32498.
UCSCi CG32498-RA. d. melanogaster. [Q9W4T4-1 ]

Organism-specific databases

CTDi 31309.
FlyBasei FBgn0000479. dnc.

Phylogenomic databases

eggNOGi NOG122287.
GeneTreei ENSGT00760000118889.
InParanoidi Q9W4T4.
KOi K13293.
OMAi INEEMET.
OrthoDBi EOG7HQNBC.
PhylomeDBi Q9W4T4.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .
Reactomei REACT_209420. G alpha (s) signalling events.
REACT_213958. DARPP-32 events.

Miscellaneous databases

ChiTaRSi dnc. drosophila.
GenomeRNAii 31309.
NextBioi 772953.

Gene expression databases

Bgeei Q9W4T4.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-524.
    Strain: Oregon-R.
  4. "Characterization of the memory gene dunce of Drosophila melanogaster."
    Qiu Y.H., Chen C.-N., Malone T., Richter L., Beckendorf S.K., Davis R.L.
    J. Mol. Biol. 222:553-565(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 495-1209, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: Canton-S1 Publication.

Entry informationi

Entry nameiPDE4A_DROME
AccessioniPrimary (citable) accession number: Q9W4T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3