Q9W4T0 (PDE4D_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase, isoform M
EC=3.1.4.17

Alternative name(s):
Learning/memory process protein
Protein dunce

Gene names

Name:	dnc
ORF Names:	CG32498

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity. Vital for female fertility. Required for learning/memory By similarity. Vital for female fertility. Required for learning/memory By similarity. UniProtKB P12252
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. UniProtKB P12252
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Monomer By similarity. UniProtKB P12252
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. UniProtKB P12252

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon extension Inferred from mutant phenotype PubMed 14960616. Source: FlyBase behavioral response to ethanol Non-traceable author statement PubMed 11715043. Source: FlyBase cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway cAMP-mediated signaling Non-traceable author statement PubMed 11092827PubMed 11462211PubMed 11741030PubMed 14515157. Source: FlyBase conditioned taste aversion Inferred from mutant phenotype PubMed 19158309. Source: FlyBase courtship behavior Traceable author statement PubMed 11092827PubMed 11715043PubMed 12486700. Source: FlyBase lateral inhibition Inferred from mutant phenotype PubMed 19363474. Source: FlyBase locomotor rhythm Non-traceable author statement PubMed 11715043. Source: FlyBase nonassociative learning Traceable author statement PubMed 14504657. Source: FlyBase olfactory learning Traceable author statement PubMed 11462211PubMed 11715043. Source: FlyBase oogenesis Traceable author statement PubMed 3089870. Source: FlyBase short-term memory Inferred from mutant phenotype PubMed 19158309. Source: FlyBase synaptic transmission Inferred from mutant phenotype PubMed 12040051PubMed 14507965. Source: FlyBase
Molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 11 isoforms produced by alternative splicing. [Align] [Select]

Isoform M Ref.1 (identifier: Q9W4T0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform F Ref.1 (identifier: Q8IRU4-1)

The sequence of this isoform can be found in the external entry Q8IRU4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform G Ref.1 (identifier: Q9W4S9-2)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform I UniProtKB P12252 (identifier: Q9W4T4-1)

Also known as: B; O;

The sequence of this isoform can be found in the external entry Q9W4T4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform II UniProtKB P12252 (identifier: P12252-1)

Also known as: I; J;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform III (identifier: P12252-7)

Also known as: E;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-429 of isoform II.

Isoform IV UniProtKB P12252 (identifier: P12252-3)

Also known as: A;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform V UniProtKB P12252 (identifier: P12252-4)

Also known as: C;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VI UniProtKB P12252 (identifier: P12252-5)

Also known as: D;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VII UniProtKB P12252 (identifier: P12252-6)

Also known as: L;

The sequence of this isoform can be found in the external entry P12252.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform N Ref.1 (identifier: Q9W4S9-1)

The sequence of this isoform can be found in the external entry Q9W4S9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 903	903	cAMP-specific 3',5'-cyclic phosphodiesterase, isoform M		PRO_0000198819
Regions
	Nucleotide binding	565 – 569	5	cAMP By similarity
Sites
	Active site	565	1	Proton donor By similarity
	Metal binding	569	1	Divalent metal cation 1 By similarity
	Metal binding	605	1	Divalent metal cation 1 By similarity
	Metal binding	606	1	Divalent metal cation 1 By similarity
	Metal binding	606	1	Divalent metal cation 2 By similarity
	Metal binding	723	1	Divalent metal cation 1 By similarity
	Binding site	606	1	cAMP By similarity
	Binding site	723	1	cAMP By similarity
	Binding site	774	1	cAMP By similarity
	Site	726	1	Binds AMP, but not cAMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform M [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 6CAC55960750C9F6	FASTA	903	97,968
	10 20 30 40 50 60 MPPPRRRRTA VAASAAAAAA ATTTTTTTTA TRAATTTPVT TAPTPTAITT TYRSRNLLKA 70 80 90 100 110 120 TAKVKKKKKW KQISFVDDQQ RLKQAATSAE RKRSTSSAPP ALESNVPPRL GRFLRQKSSE 130 140 150 160 170 180 TNSAATSAAA AAVQTEAETG RRKSSAASII AAAAGAAGLH NNNSSSCAAT ASEEQNSSGT 190 200 210 220 230 240 LTGSSGNTST STWCGSINFL VYMVCSFCCC CYNFRNSPFD VENGQGARSP LEGGSPSAGL 250 260 270 280 290 300 VLQNLPQRRE SFLYRSDSDF EMSPKSMSRN SSIASERFKE QEASILVDRS HGEDLIVTPF 310 320 330 340 350 360 AQILASLRSV RNNLLSLTNV PASNKSRRPN QSSSASRSGN PPGAPLSQGE EAYTRLATDT 370 380 390 400 410 420 IEELDWCLDQ LETIQTHRSV SDMASLKFKR MLNKELSHFS ESSRSGNQIS EYICSTFLDK 430 440 450 460 470 480 QQEFDLPSLR VEDNPELVAA NAAAGQQSAG QYARSRSPRG PPMSQISGVK RPLSHTNSFT 490 500 510 520 530 540 GERLPTFGVE TPRENELGTL LGELDTWGIQ IFSIGEFSVN RPLTCVAYTI FQSRELLTSL 550 560 570 580 590 600 MIPPKTFLNF MSTLEDHYVK DNPFHNSLHA ADVTQSTNVL LNTPALEGVF TPLEVGGALF 610 620 630 640 650 660 AACIHDVDHP GLTNQFLVNS SSELALMYND ESVLENHHLA VAFKLLQNQG CDIFCNMQKK 670 680 690 700 710 720 QRQTLRKMVI DIVLSTDMSK HMSLLADLKT MVETKKVAGS GVLLLDNYTD RIQVLENLVH 730 740 750 760 770 780 CADLSNPTKP LPLYKRWVAL LMEEFFLQGD KERESGMDIS PMCDRHNATI EKSQVGFIDY 790 800 810 820 830 840 IVHPLWETWA DLVHPDAQDI LDTLEENRDY YQSMIPPSPP PSGVDENPQE DRIRFQVTLE 850 860 870 880 890 900 ESDQENLAEL EEGDESGGES TTTGTTGTTA ASALSGAGGG GGGGGGMAPR TGGCQNQPQH GGM
	Isoform F [UniParc]. See Q8IRU4.		–	–
	Isoform G [UniParc]. See Q9W4S9.		–	–
	Isoform I (B) (O) [UniParc]. See Q9W4T4.		–	–
	Isoform II (I) (J) [UniParc]. See P12252.		–	–
	Isoform III (E) [UniParc]. See P12252.		–	–
	Isoform IV (A) [UniParc]. See P12252.		–	–
	Isoform V (C) [UniParc]. See P12252.		–	–
	Isoform VI (D) [UniParc]. See P12252.		–	–
	Isoform VII (L) [UniParc]. See P12252.		–	–
	Isoform N [UniParc]. See Q9W4S9.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF45862.2.
RefSeq	NP_001259202.1. NM_001272273.1. NP_726854.2. NM_166966.3.
UniGene	Dm.4817.
3D structure databases
ProteinModelPortal	Q9W4T0.
SMR	Q9W4T0. Positions 385-419, 494-815.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	31309.
KEGG	dme:Dmel_CG32498.
UCSC	CG32498-RA. d. melanogaster.
Organism-specific databases
CTD	31309.
FlyBase	FBgn0000479. dnc.
Phylogenomic databases
InParanoid	Q9W4T0.
KO	K01120.
OMA	TWGIEIF.
Enzyme and pathway databases
UniPathway	UPA00762; UER00747.
Gene expression databases
Bgee	Q9W4T0.
GermOnline	CG32498. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. IPR006311. TAT_signal. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	dnc. drosophila.
GenomeRNAi	31309.
NextBio	772953.

Entry information

Entry name

PDE4D_DROME

Accession

Primary (citable) accession number: Q9W4T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project