Reviewed, UniProtKB/Swiss-Prot Q9W4S9 (PDE4C_DROME)

Names and origin

Protein names

Recommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase, isoforms N/G
    EC=3.1.4.17
Alternative name(s):
    Learning/memory process protein
    Protein dunce

Gene names

Name:	dnc
ORF Names:	CG32498

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Vital for female fertility. Required for learning/memory By similarity. UniProtKB P12252
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. UniProtKB P12252
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Monomer By similarity. UniProtKB P12252
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. UniProtKB P12252

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	cAMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	associative learning Traceable author statement. Source: FlyBase axon extension Inferred from mutant phenotype. Source: FlyBase behavioral response to ethanol Non-traceable author statement. Source: FlyBase cAMP-mediated signaling Non-traceable author statement. Source: FlyBase courtship behavior Traceable author statement. Source: FlyBase locomotor rhythm Non-traceable author statement. Source: FlyBase memory Traceable author statement. Source: FlyBase nonassociative learning Traceable author statement. Source: FlyBase olfactory learning Traceable author statement. Source: FlyBase oogenesis Traceable author statement. Source: FlyBase synaptic transmission Inferred from mutant phenotype. Source: FlyBase
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 10 isoforms produced by alternative splicing. [Align] [Select]

Isoform N Ref.1 (identifier: Q9W4S9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform G Ref.1 (identifier: Q9W4S9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Note: No experimental confirmation available.

Isoform F Ref.1 (identifier: Q8IRU4-1)

The sequence of this isoform can be found in the external entry Q8IRU4-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Isoform I UniProtKB P12252 (identifier: Q9W4T4-1)

Also known as: B; O;

The sequence of this isoform can be found in the external entry Q9W4T4-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform II UniProtKB P12252 (identifier: P12252-1)

Also known as: I; J;

The sequence of this isoform can be found in the external entry P12252-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform IV UniProtKB P12252 (identifier: P12252-3)

Also known as: A;

The sequence of this isoform can be found in the external entry P12252-3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform V UniProtKB P12252 (identifier: P12252-4)

Also known as: C;

The sequence of this isoform can be found in the external entry P12252-4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VI UniProtKB P12252 (identifier: P12252-5)

Also known as: D;

The sequence of this isoform can be found in the external entry P12252-5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform VII UniProtKB P12252 (identifier: P12252-6)

Also known as: L;

The sequence of this isoform can be found in the external entry P12252-6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform M Ref.1 (identifier: Q9W4T0-1)

The sequence of this isoform can be found in the external entry Q9W4T0-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 983	983	cAMP-specific 3',5'-cyclic phosphodiesterase, isoforms N/G		PRO_0000198818
Natural variations
	Alternative sequence	1 – 169	169	Missing in isoform G. Ref.1		VSP_051775

Sequences

	Sequence		Length	Mass (Da)
	Isoform N [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 374785B501D6D73E	FASTA	983	107,341
	10 20 30 40 50 60 MIATLFIIFA FIRAFPWSRK RGRPALALTL MPEGGEDHRG DLNQKGENNN RPRPSISLAN 70 80 90 100 110 120 NGEAMAPRTR RKSSKFHEVT FSGSVGGGDS DGGGEAINGG NLDVSPSKRH SLSTTTSNSS 130 140 150 160 170 180 SAPYRYLSGS SRSRGSRGDC HEYYQLQQHS SSLSNGNRGN RGLSQRSDTM ATEAEGEEFD 190 200 210 220 230 240 VDPMDEDDED QTYDRETEEF YSNIQDAAGT GSSSRSKRSS LFSRSDSSAT TTSSSGGGTF 250 260 270 280 290 300 TGGKRRSAAS ILSSSMCSDL MTSDRRSSTA TEYSVKSVTT GNTSQRRSSG RIRRYVSRMT 310 320 330 340 350 360 IAGARRRTTG SFDVENGQGA RSPLEGGSPS AGLVLQNLPQ RRESFLYRSD SDFEMSPKSM 370 380 390 400 410 420 SRNSSIASES HGEDLIVTPF AQILASLRSV RNNLLSLTNV PASNKSRRPN QSSSASRSGN 430 440 450 460 470 480 PPGAPLSQGE EAYTRLATDT IEELDWCLDQ LETIQTHRSV SDMASLKFKR MLNKELSHFS 490 500 510 520 530 540 ESSRSGNQIS EYICSTFLDK QQEFDLPSLR VEDNPELVAA NAAAGQQSAG QYARSRSPRG 550 560 570 580 590 600 PPMSQISGVK RPLSHTNSFT GERLPTFGVE TPRENELGTL LGELDTWGIQ IFSIGEFSVN 610 620 630 640 650 660 RPLTCVAYTI FQSRELLTSL MIPPKTFLNF MSTLEDHYVK DNPFHNSLHA ADVTQSTNVL 670 680 690 700 710 720 LNTPALEGVF TPLEVGGALF AACIHDVDHP GLTNQFLVNS SSELALMYND ESVLENHHLA 730 740 750 760 770 780 VAFKLLQNQG CDIFCNMQKK QRQTLRKMVI DIVLSTDMSK HMSLLADLKT MVETKKVAGS 790 800 810 820 830 840 GVLLLDNYTD RIQVLENLVH CADLSNPTKP LPLYKRWVAL LMEEFFLQGD KERESGMDIS 850 860 870 880 890 900 PMCDRHNATI EKSQVGFIDY IVHPLWETWA DLVHPDAQDI LDTLEENRDY YQSMIPPSPP 910 920 930 940 950 960 PSGVDENPQE DRIRFQVTLE ESDQENLAEL EEGDESGGES TTTGTTGTTA ASALSGAGGG 970 980 GGGGGGMAPR TGGCQNQPQH GGM
	Isoform G. Checksum: 9185480204813E89	FASTA	814	89,085
	10 20 30 40 50 60 MATEAEGEEF DVDPMDEDDE DQTYDRETEE FYSNIQDAAG TGSSSRSKRS SLFSRSDSSA 70 80 90 100 110 120 TTTSSSGGGT FTGGKRRSAA SILSSSMCSD LMTSDRRSST ATEYSVKSVT TGNTSQRRSS 130 140 150 160 170 180 GRIRRYVSRM TIAGARRRTT GSFDVENGQG ARSPLEGGSP SAGLVLQNLP QRRESFLYRS 190 200 210 220 230 240 DSDFEMSPKS MSRNSSIASE SHGEDLIVTP FAQILASLRS VRNNLLSLTN VPASNKSRRP 250 260 270 280 290 300 NQSSSASRSG NPPGAPLSQG EEAYTRLATD TIEELDWCLD QLETIQTHRS VSDMASLKFK 310 320 330 340 350 360 RMLNKELSHF SESSRSGNQI SEYICSTFLD KQQEFDLPSL RVEDNPELVA ANAAAGQQSA 370 380 390 400 410 420 GQYARSRSPR GPPMSQISGV KRPLSHTNSF TGERLPTFGV ETPRENELGT LLGELDTWGI 430 440 450 460 470 480 QIFSIGEFSV NRPLTCVAYT IFQSRELLTS LMIPPKTFLN FMSTLEDHYV KDNPFHNSLH 490 500 510 520 530 540 AADVTQSTNV LLNTPALEGV FTPLEVGGAL FAACIHDVDH PGLTNQFLVN SSSELALMYN 550 560 570 580 590 600 DESVLENHHL AVAFKLLQNQ GCDIFCNMQK KQRQTLRKMV IDIVLSTDMS KHMSLLADLK 610 620 630 640 650 660 TMVETKKVAG SGVLLLDNYT DRIQVLENLV HCADLSNPTK PLPLYKRWVA LLMEEFFLQG 670 680 690 700 710 720 DKERESGMDI SPMCDRHNAT IEKSQVGFID YIVHPLWETW ADLVHPDAQD ILDTLEENRD 730 740 750 760 770 780 YYQSMIPPSP PPSGVDENPQ EDRIRFQVTL EESDQENLAE LEEGDESGGE STTTGTTGTT 790 800 810 AASALSGAGG GGGGGGGMAP RTGGCQNQPQ HGGM
	Isoform F. See Q8IRU4.	FASTA	–	–
	Isoform I (B) (O). See Q9W4T4.	FASTA	–	–
	Isoform II (I) (J). See P12252.	FASTA	–	–
	Isoform IV (A). See P12252.	FASTA	–	–
	Isoform V (C). See P12252.	FASTA	–	–
	Isoform VI (D). See P12252.	FASTA	–	–
	Isoform VII (L). See P12252.	FASTA	–	–
	Isoform M. See Q9W4T0.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF45863.2. AE014298 Genomic DNA. Translation: AAN09605.2.
RefSeq	NP_726856.1. NP_726858.2.
UniGene	Dm.4817
3D structure databases
HSSP	HSSP built from PDB template 1F0J based on UniProtKB Q07343.
SMR	Q9W4S9. Positions 564-896.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9W4S9.
Genome annotation databases
GeneID	31309.
UCSC	CG32498-RA. d. melanogaster.
Organism-specific databases
CTD	31309.
FlyBase	FBgn0000479. dnc.
Phylogenomic databases
OMA	CHASEMG.
Enzyme and pathway databases
BRENDA	3.1.4.17. 48.
Gene expression databases
ArrayExpress	Q9W4S9.
GermOnline	CG32498. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	772953.

Entry information

Entry name

PDE4C_DROME

Accession

Primary (citable) accession number: Q9W4S9
Secondary accession number(s): Q8IRU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project