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Protein

tRNA (cytosine(34)-C(5))-methyltransferase

Gene

Nsun2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

RNA methyltransferase that methylates tRNAs. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Required for short-term memory.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei216 – 2161S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei243 – 2431S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei270 – 2701S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei323 – 3231NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • RNA methylation Source: FlyBase
  • short-term memory Source: FlyBase
  • tRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase (EC:2.1.1.203)
Alternative name(s):
NOP2/Sun domain family member 2 ortholog
Gene namesi
Name:Nsun2
ORF Names:CG6133
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0026079. Nsun2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746tRNA (cytosine(34)-C(5))-methyltransferasePRO_0000289228Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9W4M9.
PRIDEiQ9W4M9.

Expressioni

Tissue specificityi

Ubiquitously expressed during embryonic development. Some enrichment is observed in the proventriculus area of the foregut and in the hindgut.1 Publication

Developmental stagei

Expressed maternally and zygotically. Expressed during embryonic development.1 Publication

Gene expression databases

BgeeiQ9W4M9.
GenevisibleiQ9W4M9. DM.

Interactioni

Protein-protein interaction databases

BioGridi69457. 2 interactions.
IntActiQ9W4M9. 2 interactions.
MINTiMINT-1331472.
STRINGi7227.FBpp0070637.

Structurei

3D structure databases

ProteinModelPortaliQ9W4M9.
SMRiQ9W4M9. Positions 152-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1907S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
GeneTreeiENSGT00660000095589.
InParanoidiQ9W4M9.
KOiK15335.
OMAiPWGPQRK.
OrthoDBiEOG72VH5D.
PhylomeDBiQ9W4M9.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W4M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRNQKQNFF AARKRQKREN GPKRTDRQAQ PYEEIKRDNA FFIKYYQLQK
60 70 80 90 100
ICATDEEWTQ FLASIRDNLP TTFRVTGFKD EAKALLSIIE TQLFTEYVRA
110 120 130 140 150
VAELHQKAPE DVERPLCLPW YPNGLAYQLH LTRKDIRRSE PLYRLHNFLI
160 170 180 190 200
VETTAGGISR QEAVSMIPPI VLDVRPTDKV LDMCAAPGSK TAQLIEALHA
210 220 230 240 250
APEEHKIPPG FVLANDVDNN RCYMLVHQAK RLNSPCLLVT NHDSSVFPNL
260 270 280 290 300
VTTKPDGSKA ILKFDKILCD VPCSGDGTLR KNPDIWLKWN LAQAYNLHGI
310 320 330 340 350
QYRIVRRGAE MLEVGGRLVY STCSLNPIEN EAVLQRIIKD ADGALELVDA
360 370 380 390 400
GHLVPGLKYK PGMTDWKLAT KEVDQIFTRF EEVPESLHTI IRPGMFPLPA
410 420 430 440 450
DEMAKIGLEK CLRVLPHLQD SGGFFVAVLE KRRQLSFEKN DVVELVKLNE
460 470 480 490 500
TAKQPAAEPQ VDADGKPIEE KSVPWGPQRK KRRLHGYKED PYVFFGENDP
510 520 530 540 550
DYQAIKEFYQ LDESLSQRCL LTRCVTEKKK NIYYCSEPIR DLVLNNENNI
560 570 580 590 600
KIINTGVKTF VRCENRHTVH PFRLAQEGLQ TSNAFMGASR RIQVEREDLV
610 620 630 640 650
MMLNCTDPTQ PPSTHELKKE TQERCKELGV GSCILKYVDQ RFTLYTVGWR
660 670 680 690 700
GTSSLRAYVQ KDETIHILRL LGADLSKFET NKYEDARVAA AAAADAEVGK
710 720 730 740
SAEAEADSSG DGDATESTFS GSGAIDVTVA AETTGTPMDT EVVATS
Length:746
Mass (Da):84,162
Last modified:May 1, 2000 - v1
Checksum:i5178D0D26097C5F0
GO

Sequence cautioni

The sequence CAA21831.1 differs from that shown. Reason: Frameshift at position 709. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45921.1.
AL033125 Genomic DNA. Translation: CAA21831.1. Frameshift.
AY061496 mRNA. Translation: AAL29044.1.
RefSeqiNP_652007.1. NM_143750.3.
UniGeneiDm.3580.

Genome annotation databases

EnsemblMetazoaiFBtr0070669; FBpp0070637; FBgn0026079.
GeneIDi45064.
KEGGidme:Dmel_CG6133.
UCSCiCG6133-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45921.1.
AL033125 Genomic DNA. Translation: CAA21831.1. Frameshift.
AY061496 mRNA. Translation: AAL29044.1.
RefSeqiNP_652007.1. NM_143750.3.
UniGeneiDm.3580.

3D structure databases

ProteinModelPortaliQ9W4M9.
SMRiQ9W4M9. Positions 152-431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69457. 2 interactions.
IntActiQ9W4M9. 2 interactions.
MINTiMINT-1331472.
STRINGi7227.FBpp0070637.

Proteomic databases

PaxDbiQ9W4M9.
PRIDEiQ9W4M9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070669; FBpp0070637; FBgn0026079.
GeneIDi45064.
KEGGidme:Dmel_CG6133.
UCSCiCG6133-RA. d. melanogaster.

Organism-specific databases

CTDi54888.
FlyBaseiFBgn0026079. Nsun2.

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
GeneTreeiENSGT00660000095589.
InParanoidiQ9W4M9.
KOiK15335.
OMAiPWGPQRK.
OrthoDBiEOG72VH5D.
PhylomeDBiQ9W4M9.

Miscellaneous databases

GenomeRNAii45064.
PROiQ9W4M9.

Gene expression databases

BgeeiQ9W4M9.
GenevisibleiQ9W4M9. DM.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oregon-R.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiNSUN2_DROME
AccessioniPrimary (citable) accession number: Q9W4M9
Secondary accession number(s): O97427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.