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Protein

Josephin-like protein

Gene

CG3781

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

May act as a deubiquitinating enzyme.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49NucleophilePROSITE-ProRule annotation1
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-5689877. Josephin domain DUBs.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-like protein (EC:3.4.19.12)
Gene namesi
ORF Names:CG3781
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0029853. CG3781.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538451 – 221Josephin-like proteinAdd BLAST221

Proteomic databases

PaxDbiQ9W422.
PRIDEiQ9W422.

Expressioni

Gene expression databases

BgeeiFBgn0029853.
ExpressionAtlasiQ9W422. baseline.
GenevisibleiQ9W422. DM.

Interactioni

Protein-protein interaction databases

BioGridi58051. 1 interactor.
DIPiDIP-23119N.
MINTiMINT-966633.
STRINGi7227.FBpp0070860.

Structurei

3D structure databases

ProteinModelPortaliQ9W422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 214JosephinPROSITE-ProRule annotationAdd BLAST179

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
InParanoidiQ9W422.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ9W422.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESPSARTLG NSLGDDSGNG NENGNGSGNG NTTMMPHGIY HERQTRHLCG
60 70 80 90 100
LHALNNLFQG PDMFSKSELD DYCTTLTPRN WLNPHRSWIG WGNYDVNVIM
110 120 130 140 150
YALQQRNCEA VWFDRRRDPH CLNLSVIFGF ILNVPAQMSL GYYIPLPFHM
160 170 180 190 200
RHWLALRRLN GSYYNLDSKL REPKCLGTEQ QFLEFLATQL QMDHELFLVL
210 220
DEETDCKDKS QQRWLLPQFR D
Length:221
Mass (Da):25,656
Last modified:March 1, 2003 - v3
Checksum:i17202EB46E97EFAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43R → L in AAL48447 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF46138.3.
AY070825 mRNA. Translation: AAL48447.2.
RefSeqiNP_572303.4. NM_132075.4.
UniGeneiDm.5285.

Genome annotation databases

EnsemblMetazoaiFBtr0070895; FBpp0070860; FBgn0029853.
GeneIDi31560.
KEGGidme:Dmel_CG3781.
UCSCiCG3781-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF46138.3.
AY070825 mRNA. Translation: AAL48447.2.
RefSeqiNP_572303.4. NM_132075.4.
UniGeneiDm.5285.

3D structure databases

ProteinModelPortaliQ9W422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58051. 1 interactor.
DIPiDIP-23119N.
MINTiMINT-966633.
STRINGi7227.FBpp0070860.

Proteomic databases

PaxDbiQ9W422.
PRIDEiQ9W422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070895; FBpp0070860; FBgn0029853.
GeneIDi31560.
KEGGidme:Dmel_CG3781.
UCSCiCG3781-RA. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0029853. CG3781.

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
InParanoidiQ9W422.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ9W422.

Enzyme and pathway databases

ReactomeiR-DME-5689877. Josephin domain DUBs.

Miscellaneous databases

GenomeRNAii31560.
PROiQ9W422.

Gene expression databases

BgeeiFBgn0029853.
ExpressionAtlasiQ9W422. baseline.
GenevisibleiQ9W422. DM.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJOSL_DROME
AccessioniPrimary (citable) accession number: Q9W422
Secondary accession number(s): Q8MTV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.