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Protein

Transcription elongation factor SPT6

Gene

Spt6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription elongation factor which binds histone H3 and enhances transcription elongation by RNA polymerase II (RNAPII). Required for the transcriptional induction of heat shock response genes and for maximal recruitment of two other elongation factors, Spt5 and Paf1, to the induced Hsp70. Plays a critical role in normal fly development throughout the lifecycle.1 Publication

GO - Molecular functioni

GO - Biological processi

  • chromatin maintenance Source: GO_Central
  • chromatin silencing Source: GO_Central
  • mRNA transcription from RNA polymerase II promoter Source: GO_Central
  • nucleosome organization Source: GO_Central
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: FlyBase
  • regulation of antimicrobial peptide biosynthetic process Source: FlyBase
  • regulation of mRNA processing Source: GO_Central
  • transcription from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Molecular functionChaperone
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-112382. Formation of RNA Pol II elongation complex.
R-DME-674695. RNA Polymerase II Pre-transcription Events.
R-DME-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Gene namesi
Name:Spt6
ORF Names:CG12225
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0028982. Spt6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721701 – 1831Transcription elongation factor SPT6Add BLAST1831

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34Phosphoserine1 Publication1
Modified residuei36Phosphoserine1 Publication1
Modified residuei37Phosphoserine1 Publication1
Modified residuei66Phosphoserine1 Publication1
Modified residuei70Phosphoserine1 Publication1
Modified residuei75Phosphoserine1 Publication1
Modified residuei124Phosphoserine1 Publication1
Modified residuei151Phosphoserine1 Publication1
Modified residuei156Phosphoserine1 Publication1
Modified residuei159Phosphoserine1 Publication1
Modified residuei171Phosphothreonine1 Publication1
Modified residuei173Phosphoserine1 Publication1
Modified residuei1661Phosphoserine1 Publication1
Modified residuei1664Phosphoserine1 Publication1
Modified residuei1815Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9W420.
PRIDEiQ9W420.

PTM databases

iPTMnetiQ9W420.

Expressioni

Gene expression databases

BgeeiFBgn0028982.
GenevisibleiQ9W420. DM.

Interactioni

Subunit structurei

Self associates. Interacts with RNA polymerase II. Interacts with the FACT complex, which is composed of dre4/Spt16 and Ssrp/Ssrp1. Interacts with the exosome, a complex with 3'-5' exoribonuclease activity which is composed of at least Csl4, Dis3, Mtr3, Rrp4, Rrp6, Rrp40, Rrp42, Rrp46 and Ski6. Interacts with the DRB sensitivity-inducing factor complex (the DSIF complex), which is composed of Spt4 and Spt5.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68765. 11 interactors.
IntActiQ9W420. 8 interactors.
STRINGi7227.FBpp0070861.

Structurei

3D structure databases

ProteinModelPortaliQ9W420.
SMRiQ9W420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1217 – 1286S1 motifPROSITE-ProRule annotationAdd BLAST70
Domaini1329 – 1440SH2Add BLAST112

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili7 – 59Sequence analysisAdd BLAST53
Coiled coili467 – 494Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 245Asp/Glu-richAdd BLAST240
Compositional biasi489 – 512Glu-richAdd BLAST24
Compositional biasi1552 – 1682Ser-richAdd BLAST131
Compositional biasi1675 – 1702Gly-richAdd BLAST28
Compositional biasi1771 – 1783Poly-GlyAdd BLAST13

Sequence similaritiesi

Belongs to the SPT6 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1856. Eukaryota.
COG2183. LUCA.
GeneTreeiENSGT00510000047446.
InParanoidiQ9W420.
KOiK11292.
OMAiEADWIYR.
OrthoDBiEOG091G00SW.
PhylomeDBiQ9W420.

Family and domain databases

CDDicd09928. SH2_Cterm_SPT6_like. 1 hit.
cd09918. SH2_Nterm_SPT6_like. 1 hit.
Gene3Di1.10.10.650. 1 hit.
1.10.3500.10. 1 hit.
3.30.420.140. 1 hit.
3.30.505.10. 2 hits.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR035420. Spt6_SH2.
IPR035018. Spt6_SH2_C.
IPR035019. Spt6_SH2_N.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023319. Tex-like_HTH_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
PANTHERiPTHR10145:SF7. PTHR10145:SF7. 1 hit.
PfamiView protein in Pfam
PF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF00575. S1. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiView protein in SMART
SM00316. S1. 1 hit.
SM00732. YqgFc. 1 hit.
SUPFAMiSSF47781. SSF47781. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiView protein in PROSITE
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9W420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFLDSEAE ESEEEEELDV NERKRLKKLK AAVSDSSEEE EDDEERLREE
60 70 80 90 100
LKDLIDDNPI EEDDGSGYDS DGVGSGKKRK KHEDDDLDDR LEDDDYDLIE
110 120 130 140 150
ENLGVKVERR KRFKRLRRIH DNESDGEEQH VDEGLVREQI AEQLFDENDE
160 170 180 190 200
SIGHRSERSH READDYDDVD TESDADDFIV DDNGRPIAEK KKKRRPIFTD
210 220 230 240 250
ASLQEGQDIF GVDFDYDDFS KYEEDDYEDD SEGDEYDEDL GVGDDTRVKK
260 270 280 290 300
KKALKKKVVK KTIFDIYEPS ELKRGHFTDM DNEIRKTDIP ERMQLREVPV
310 320 330 340 350
TPVPEGSDEL DLEAEWIYKY AFCKHTVSEQ EKPESREKMR KPPTTVNKIK
360 370 380 390 400
QTLEFIRNQQ LEVPFIAFYR KEYVKPELNI DDLWKVYYYD GIWCQLNERK
410 420 430 440 450
RKLKVLFEKM RQFQLDTLCA DTDQPVPDDV RLILDSDFER LADVQSMEEL
460 470 480 490 500
KDVHMYFLLN YSHELPRMQA EQRRKAIQER REAKARRQAA AAENGDDAAE
510 520 530 540 550
AIVVPEPEDD DDPELIDYQL KQASNSSPYA VFRKAGICGF AKHFGLTPEQ
560 570 580 590 600
YAENLRDNYQ RNEITQESIG PTELAKQYLS PRFMTTDEVI HAAKYVVARQ
610 620 630 640 650
LAQEPLLRKT MREVYFDRAR INIRPTKNGM VLIDENSPVY SMKYVAKKPV
660 670 680 690 700
SDLFGDQFIK LMMAEEEKLL EITFLEEFEG NACANGTPGD YVEESKALYQ
710 720 730 740 750
LDQFAKHVQE WNKLRAECVQ LALQKWVIPD LIKELRSTLH EEAQQFVLRS
760 770 780 790 800
CTGKLYKWLK VAPYKPQLPP DFGYEEWSTL RGIRVLGLAY DPDHSVAAFC
810 820 830 840 850
AVTTVEGDIS DYLRLPNILK RKNSYNLEEK AQKLADLRKL SDFIKMKKPH
860 870 880 890 900
IVVIGAESRD AQNIQADIKE ILHELETSEQ FPPIEVEIID NELAKIYANS
910 920 930 940 950
KKGESDFKEY PPLLKQAASL ARKMQDPLVE YSQLCDADDE ILCLRYHPLQ
960 970 980 990 1000
ERVPREQLLE QLSLQFINRT SEVGLDINLM VQNSRTINLL QYICGLGPRK
1010 1020 1030 1040 1050
GQALLKLLKQ SNQRLENRTQ LVTVCHLGPR VFINCSGFIK IDTSSLGDST
1060 1070 1080 1090 1100
EAYVEVLDGS RVHPETYEWA RKMAIDAMEY DDEETNPAGA LEEILESPER
1110 1120 1130 1140 1150
LKDLDLDAFA VELERQGFGS KSITLYDIRN ELSCLYKDYR TPYTKPSAEE
1160 1170 1180 1190 1200
LFDMLTKETP DSFYVGKCVT AMVTGFTYRR PQGDQLDSAN PVRLDSNESW
1210 1220 1230 1240 1250
QCPFCHKDDF PELSEVWNHF DANACPGQPS GVRVRLENGL PGFIHIKNLS
1260 1270 1280 1290 1300
DRQVRNPEER VRVSQMIHVR IIKIDIDRFS VECSSRTADL KDVNNEWRPR
1310 1320 1330 1340 1350
RDNYYDYVTE EQDNRKVSDA KARALKRKIY ARRVIAHPSF FNKSYAEVVA
1360 1370 1380 1390 1400
MLAEADQGEV ALRPSSKSKD HLTATWKVAD DIFQHIDVRE EGKENDFSLG
1410 1420 1430 1440 1450
RSLWIGTEEF EDLDEIIARH IMPMALAARE LIQYKYYKPN MVTGDENERD
1460 1470 1480 1490 1500
VMEKLLREEK ANDPKKIHYF FTASRAMPGK FLLSYLPKTK VRHEYVTVMP
1510 1520 1530 1540 1550
EGYRFRGQIF DTVNSLLRWF KEHWLDPTAT PASASASNLT PLHLMRPPPT
1560 1570 1580 1590 1600
ISSSSQTSLG PQAPYSVTGS VTGGTPRSGI SSAVGGGGSS AYSITQSITG
1610 1620 1630 1640 1650
YGTSGSSAPG AGVSSSHYGS SSTPSFGAIN TPYTPSGQTP FMTPYTPHAS
1660 1670 1680 1690 1700
QTPRYGHNVP SPSSQSSSSQ RHHYGSSSGT GSTPRYHDMG GGGGGGVGGG
1710 1720 1730 1740 1750
GGSNAYSMQP HHQQRAKENL DWQLANDAWA RRRPQQHQSH QSYHAQQQHH
1760 1770 1780 1790 1800
HSQQQPHMGM SMNMGITMSL GRGTGGGGGG GYGSTPVNDY STGGGHNRGM
1810 1820 1830
SSKASVRSTP RTNASPHSMN LGDATPLYDE N
Length:1,831
Mass (Da):208,599
Last modified:May 1, 2000 - v1
Checksum:i005FC78C83EF744D
GO

Sequence cautioni

The sequence AAF14115 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAK93454 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1194L → I in AAF14114 (Ref. 1) Curated1
Sequence conflicti1194L → I in AAF14115 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104400 mRNA. Translation: AAF14114.1.
AF104401 Genomic DNA. Translation: AAF14115.1. Sequence problems.
AE014298 Genomic DNA. Translation: AAF46140.1.
AY052030 mRNA. Translation: AAK93454.1. Different initiation.
RefSeqiNP_001284936.1. NM_001298007.1.
NP_651962.2. NM_143705.3.

Genome annotation databases

EnsemblMetazoaiFBtr0070896; FBpp0070861; FBgn0028982.
FBtr0340027; FBpp0309041; FBgn0028982.
GeneIDi44000.
KEGGidme:Dmel_CG12225.

Similar proteinsi

Entry informationi

Entry nameiSPT6H_DROME
AccessioniPrimary (citable) accession number: Q9W420
Secondary accession number(s): Q960J2, Q9U8B5, Q9U8B6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families