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Q9W401

- CISY_DROME

UniProt

Q9W401 - CISY_DROME

Protein

Probable citrate synthase, mitochondrial

Gene

kdn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Plays a role in controlling neuronal activity and seizure susceptibility.1 Publication

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei302 – 3021PROSITE-ProRule annotation
    Active sitei348 – 3481PROSITE-ProRule annotation
    Active sitei403 – 4031PROSITE-ProRule annotation

    GO - Molecular functioni

    1. citrate (Si)-synthase activity Source: UniProtKB

    GO - Biological processi

    1. behavior Source: UniProtKB-KW
    2. carbohydrate metabolic process Source: UniProtKB
    3. cellular carbohydrate metabolic process Source: InterPro
    4. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Behavior, Tricarboxylic acid cycle

    Enzyme and pathway databases

    ReactomeiREACT_218888. Citric acid cycle (TCA cycle).
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable citrate synthase, mitochondrial (EC:2.3.3.16)
    Alternative name(s):
    Protein knockdown
    Gene namesi
    Name:kdn
    Synonyms:l(1)G0030
    ORF Names:CG3861
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0261955. kdn.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. microtubule associated complex Source: FlyBase
    2. mitochondrial matrix Source: UniProtKB
    3. mitochondrion Source: FlyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 464Probable citrate synthase, mitochondrialPRO_0000291603
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    PaxDbiQ9W401.
    PRIDEiQ9W401.

    Expressioni

    Gene expression databases

    BgeeiQ9W401.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi58066. 3 interactions.
    MINTiMINT-936064.
    STRINGi7227.FBpp0070871.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9W401.
    SMRiQ9W401. Positions 29-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0372.
    GeneTreeiENSGT00390000006813.
    InParanoidiQ9W401.
    KOiK01647.
    OMAiELIYEDC.
    OrthoDBiEOG73BVCB.
    PhylomeDBiQ9W401.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q9W401-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLYRISARK LSEAQKLPNV GAYVRMIAAD GKSLRDVLAA KVPQEQERVK    50
    NFRKQHGATK MGETTIDMMY GGMRGIKALV TETSVLDADE GIRFRGLSIP 100
    ECQKVLPAAD GGTEPLPEGL FWLLLTGEVP TKSQVQQLSR EWAERAALPQ 150
    HVVTMLNNMP TTLHPMSQFA AAVTALNHDS KFAKAYSDGV HKSKYWEYVY 200
    EDSMDLIAKL PVVAATIYCN TYRGGKGSRS IDSSLDWSAN FVKMLGYDNA 250
    PFTELMRLYL TIHSDHEGGN VSAHTVHLVG SALSDPYLSF AAGLNGLAGP 300
    LHGLANQEVL VWLRKLQKEA GNNPSEEQLK EYIWKTLKSG QVVPGYGHAV 350
    LRKTDPRYTC QREFALKHLP EDETFQLVSK IYKVVPPILT ETGKVKNPWP 400
    NVDAHSGVLL QYYGMKEMNY YTVLFGVSRA LGVLASLVWD RALGLPIERP 450
    KSFSTDLLVK MVQK 464

    Note: No experimental confirmation available.

    Length:464
    Mass (Da):51,574
    Last modified:May 1, 2000 - v1
    Checksum:i57D582652931B4F9
    GO
    Isoform B (identifier: Q9W401-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-13: SLYRISARKLSE → FVRRFGNGSL...ETLNMPDMQD

    Note: No experimental confirmation available.

    Show »
    Length:522
    Mass (Da):58,217
    Checksum:i7CA9D79350F2764D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 1312SLYRI…RKLSE → FVRRFGNGSLGHHFAFQRRY LAKNSMWDSKSIGTPSSFRM RSKKDNVTGNLKEQQQSATP ETLNMPDMQD in isoform B. 1 PublicationVSP_027491Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF46159.1.
    AE014298 Genomic DNA. Translation: AAN09169.1.
    AY089318 mRNA. Translation: AAL90056.1.
    RefSeqiNP_572319.2. NM_132091.2. [Q9W401-1]
    NP_727091.1. NM_167072.1. [Q9W401-2]
    UniGeneiDm.8.

    Genome annotation databases

    EnsemblMetazoaiFBtr0070907; FBpp0070872; FBgn0261955. [Q9W401-1]
    GeneIDi31579.
    KEGGidme:Dmel_CG3861.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014298 Genomic DNA. Translation: AAF46159.1 .
    AE014298 Genomic DNA. Translation: AAN09169.1 .
    AY089318 mRNA. Translation: AAL90056.1 .
    RefSeqi NP_572319.2. NM_132091.2. [Q9W401-1 ]
    NP_727091.1. NM_167072.1. [Q9W401-2 ]
    UniGenei Dm.8.

    3D structure databases

    ProteinModelPortali Q9W401.
    SMRi Q9W401. Positions 29-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 58066. 3 interactions.
    MINTi MINT-936064.
    STRINGi 7227.FBpp0070871.

    Proteomic databases

    PaxDbi Q9W401.
    PRIDEi Q9W401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0070907 ; FBpp0070872 ; FBgn0261955 . [Q9W401-1 ]
    GeneIDi 31579.
    KEGGi dme:Dmel_CG3861.

    Organism-specific databases

    CTDi 31579.
    FlyBasei FBgn0261955. kdn.

    Phylogenomic databases

    eggNOGi COG0372.
    GeneTreei ENSGT00390000006813.
    InParanoidi Q9W401.
    KOi K01647.
    OMAi ELIYEDC.
    OrthoDBi EOG73BVCB.
    PhylomeDBi Q9W401.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .
    Reactomei REACT_218888. Citric acid cycle (TCA cycle).

    Miscellaneous databases

    GenomeRNAii 31579.
    NextBioi 774282.
    PROi Q9W401.

    Gene expression databases

    Bgeei Q9W401.

    Family and domain databases

    Gene3Di 1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Testis.
    4. Cited for: IDENTIFICATION.
    5. "Metabolic disruption in Drosophila bang-sensitive seizure mutants."
      Fergestad T., Bostwick B., Ganetzky B.
      Genetics 173:1357-1364(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCISY_DROME
    AccessioniPrimary (citable) accession number: Q9W401
    Secondary accession number(s): Q8T477
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.
    Mutation of kdn causes a behavioral mutation ('bang sensitivity' = temporarily paralysis in response to a physical jolt). Flies lacking kdn exhibit alterations in neuronal firing patterns in the giant fiber (GF) pathway.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3