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Protein

Probable citrate synthase, mitochondrial

Gene

kdn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a role in controlling neuronal activity and seizure susceptibility.1 Publication

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Mutation of kdn causes a behavioral mutation ('bang sensitivity' = temporarily paralysis in response to a physical jolt). Flies lacking kdn exhibit alterations in neuronal firing patterns in the giant fiber (GF) pathway.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable citrate synthase, mitochondrial (kdn)
  2. no protein annotated in this organism
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei302PROSITE-ProRule annotation1
Active sitei348PROSITE-ProRule annotation1
Active sitei403PROSITE-ProRule annotation1

GO - Molecular functioni

  • citrate (Si)-synthase activity Source: FlyBase

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBehavior, Tricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-DME-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Probable citrate synthase, mitochondrial (EC:2.3.3.16)
Alternative name(s):
Protein knockdown
Gene namesi
Name:kdn
Synonyms:l(1)G0030
ORF Names:CG3861
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0261955 kdn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000291603? – 464Probable citrate synthase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ9W401
PRIDEiQ9W401

Expressioni

Gene expression databases

BgeeiFBgn0261955
ExpressionAtlasiQ9W401 differential
GenevisibleiQ9W401 DM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi58066, 45 interactors
IntActiQ9W401, 28 interactors
STRINGi7227.FBpp0070871

Structurei

3D structure databases

ProteinModelPortaliQ9W401
SMRiQ9W401
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
GeneTreeiENSGT00390000006813
InParanoidiQ9W401
KOiK01647
OMAiTVGWCAQ
OrthoDBiEOG091G068F
PhylomeDBiQ9W401

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9W401-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLYRISARK LSEAQKLPNV GAYVRMIAAD GKSLRDVLAA KVPQEQERVK
60 70 80 90 100
NFRKQHGATK MGETTIDMMY GGMRGIKALV TETSVLDADE GIRFRGLSIP
110 120 130 140 150
ECQKVLPAAD GGTEPLPEGL FWLLLTGEVP TKSQVQQLSR EWAERAALPQ
160 170 180 190 200
HVVTMLNNMP TTLHPMSQFA AAVTALNHDS KFAKAYSDGV HKSKYWEYVY
210 220 230 240 250
EDSMDLIAKL PVVAATIYCN TYRGGKGSRS IDSSLDWSAN FVKMLGYDNA
260 270 280 290 300
PFTELMRLYL TIHSDHEGGN VSAHTVHLVG SALSDPYLSF AAGLNGLAGP
310 320 330 340 350
LHGLANQEVL VWLRKLQKEA GNNPSEEQLK EYIWKTLKSG QVVPGYGHAV
360 370 380 390 400
LRKTDPRYTC QREFALKHLP EDETFQLVSK IYKVVPPILT ETGKVKNPWP
410 420 430 440 450
NVDAHSGVLL QYYGMKEMNY YTVLFGVSRA LGVLASLVWD RALGLPIERP
460
KSFSTDLLVK MVQK
Note: No experimental confirmation available.
Length:464
Mass (Da):51,574
Last modified:May 1, 2000 - v1
Checksum:i57D582652931B4F9
GO
Isoform B (identifier: Q9W401-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-13: SLYRISARKLSE → FVRRFGNGSL...ETLNMPDMQD

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):58,217
Checksum:i7CA9D79350F2764D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0274912 – 13SLYRI…RKLSE → FVRRFGNGSLGHHFAFQRRY LAKNSMWDSKSIGTPSSFRM RSKKDNVTGNLKEQQQSATP ETLNMPDMQD in isoform B. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAF46159.1
AE014298 Genomic DNA Translation: AAN09169.1
AY089318 mRNA Translation: AAL90056.1
RefSeqiNP_572319.2, NM_132091.3 [Q9W401-1]
NP_727091.1, NM_167072.2 [Q9W401-2]
UniGeneiDm.8

Genome annotation databases

EnsemblMetazoaiFBtr0070906; FBpp0070871; FBgn0261955 [Q9W401-2]
FBtr0070907; FBpp0070872; FBgn0261955 [Q9W401-1]
GeneIDi31579
KEGGidme:Dmel_CG3861

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCISY_DROME
AccessioniPrimary (citable) accession number: Q9W401
Secondary accession number(s): Q8T477
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health