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Q9W401 (CISY_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable citrate synthase, mitochondrial

EC=2.3.3.16
Alternative name(s):
Protein knockdown
Gene names
Name:kdn
Synonyms:l(1)G0030
ORF Names:CG3861
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in controlling neuronal activity and seizure susceptibility. Ref.5

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Mutation of kdn causes a behavioral mutation ('bang sensitivity' = temporarily paralysis in response to a physical jolt). Flies lacking kdn exhibit alterations in neuronal firing patterns in the giant fiber (GF) pathway.

Sequence similarities

Belongs to the citrate synthase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9W401-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: Q9W401-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-13: SLYRISARKLSE → FVRRFGNGSL...ETLNMPDMQD
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 464Probable citrate synthase, mitochondrialPRO_0000291603

Sites

Active site3021 By similarity
Active site3481 By similarity
Active site4031 By similarity

Natural variations

Alternative sequence2 – 1312SLYRI…RKLSE → FVRRFGNGSLGHHFAFQRRY LAKNSMWDSKSIGTPSSFRM RSKKDNVTGNLKEQQQSATP ETLNMPDMQD in isoform B.
VSP_027491

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 57D582652931B4F9

FASTA46451,574
        10         20         30         40         50         60 
MSLYRISARK LSEAQKLPNV GAYVRMIAAD GKSLRDVLAA KVPQEQERVK NFRKQHGATK 

        70         80         90        100        110        120 
MGETTIDMMY GGMRGIKALV TETSVLDADE GIRFRGLSIP ECQKVLPAAD GGTEPLPEGL 

       130        140        150        160        170        180 
FWLLLTGEVP TKSQVQQLSR EWAERAALPQ HVVTMLNNMP TTLHPMSQFA AAVTALNHDS 

       190        200        210        220        230        240 
KFAKAYSDGV HKSKYWEYVY EDSMDLIAKL PVVAATIYCN TYRGGKGSRS IDSSLDWSAN 

       250        260        270        280        290        300 
FVKMLGYDNA PFTELMRLYL TIHSDHEGGN VSAHTVHLVG SALSDPYLSF AAGLNGLAGP 

       310        320        330        340        350        360 
LHGLANQEVL VWLRKLQKEA GNNPSEEQLK EYIWKTLKSG QVVPGYGHAV LRKTDPRYTC 

       370        380        390        400        410        420 
QREFALKHLP EDETFQLVSK IYKVVPPILT ETGKVKNPWP NVDAHSGVLL QYYGMKEMNY 

       430        440        450        460 
YTVLFGVSRA LGVLASLVWD RALGLPIERP KSFSTDLLVK MVQK 

« Hide

Isoform B [UniParc].

Checksum: 7CA9D79350F2764D
Show »

FASTA52258,217

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Testis.
[4]"Drosophila melanogaster larval hemolymph protein mapping."
Guedes Sde M., Vitorino R., Tomer K., Domingues M.R., Correia A.J., Amado F., Domingues P.
Biochem. Biophys. Res. Commun. 312:545-554(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Metabolic disruption in Drosophila bang-sensitive seizure mutants."
Fergestad T., Bostwick B., Ganetzky B.
Genetics 173:1357-1364(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014298 Genomic DNA. Translation: AAF46159.1.
AE014298 Genomic DNA. Translation: AAN09169.1.
AY089318 mRNA. Translation: AAL90056.1.
RefSeqNP_572319.2. NM_132091.2. [Q9W401-1]
NP_727091.1. NM_167072.1. [Q9W401-2]
UniGeneDm.8.

3D structure databases

ProteinModelPortalQ9W401.
SMRQ9W401. Positions 29-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58066. 3 interactions.
MINTMINT-936064.
STRING7227.FBpp0070871.

Proteomic databases

PaxDbQ9W401.
PRIDEQ9W401.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0070907; FBpp0070872; FBgn0261955. [Q9W401-1]
GeneID31579.
KEGGdme:Dmel_CG3861.

Organism-specific databases

CTD31579.
FlyBaseFBgn0261955. kdn.

Phylogenomic databases

eggNOGCOG0372.
GeneTreeENSGT00390000006813.
InParanoidQ9W401.
KOK01647.
OMAELIYEDC.
OrthoDBEOG73BVCB.
PhylomeDBQ9W401.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Gene expression databases

BgeeQ9W401.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01793. cit_synth_euk. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi31579.
NextBio774282.
PROQ9W401.

Entry information

Entry nameCISY_DROME
AccessionPrimary (citable) accession number: Q9W401
Secondary accession number(s): Q8T477
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase