Q9W3V8 (ALG8_DROME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 77.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase Short name=Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase EC=2.4.1.265 Alternative name(s): Asparagine-linked glycosylation protein 8 homolog Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase | ||
| Gene names |
| ||
| Organism | Drosophila melanogaster (Fruit fly) | ||
| Taxonomic identifier | 7227 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 511 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity. |
| Catalytic activity | Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. |
| Sequence similarities | Belongs to the ALG6/ALG8 glucosyltransferase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 511 | 511 | Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase | PRO_0000174165 | |||||
Regions | |||||||||
| Transmembrane | 4 – 24 | 21 | Helical; Potential | ||||||
| Transmembrane | 94 – 112 | 19 | Helical; Potential | ||||||
| Transmembrane | 124 – 144 | 21 | Helical; Potential | ||||||
| Transmembrane | 151 – 171 | 21 | Helical; Potential | ||||||
| Transmembrane | 210 – 230 | 21 | Helical; Potential | ||||||
| Transmembrane | 300 – 320 | 21 | Helical; Potential | ||||||
| Transmembrane | 332 – 352 | 21 | Helical; Potential | ||||||
| Transmembrane | 356 – 370 | 15 | Helical; Potential | ||||||
| Transmembrane | 377 – 394 | 18 | Helical; Potential | ||||||
| Transmembrane | 432 – 452 | 21 | Helical; Potential | ||||||
| Transmembrane | 469 – 491 | 23 | Helical; Potential | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014298 Genomic DNA. Translation: AAF46205.1. AY069662 mRNA. Translation: AAL39807.1. BT029072 mRNA. Translation: ABJ17005.1. |
| RefSeq | NP_572355.1. NM_132127.3. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9W3V8. |
Protein family/group databases | |
| CAZy | GT57. Glycosyltransferase Family 57. |
Proteomic databases | |
| PRIDE | Q9W3V8. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | FBtr0070970; FBpp0070931; FBgn0029906. |
| GeneID | 31623. |
| KEGG | dme:Dmel_CG4542. |
| NMPDR | fig|7227.3.peg.16783. |
| UCSC | CG4542-RA. d. melanogaster. |
Organism-specific databases | |
| FlyBase | FBgn0029906. CG4542. |
Phylogenomic databases | |
| eggNOG | inNOG07583. |
| GeneTree | EMGT00050000012559. |
| InParanoid | Q9W3V8. |
| OMA | HNLNYSS. |
| OrthoDB | EOG4TTF0P. |
| PhylomeDB | Q9W3V8. |
Gene expression databases | |
| GermOnline | CG4542. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR004856. Glyco_trans_ALG6/ALG8. [Graphical view] |
| KO | K03849. |
| PANTHER | PTHR12413. Alg6_Alg8. 1 hit. |
| Pfam | PF03155. Alg6_Alg8. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 774548. |
Entry information
| Entry name | ALG8_DROME | ||||||||
| Accession | Primary (citable) accession number: Q9W3V8 Secondary accession number(s): Q058Z6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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