Q9W349 (LOZEN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lozenge

Gene names

Name:	lz
ORF Names:	CG1689

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in prepatterning photoreceptor precursors in the developing eye; in the larval eye disk it defines a subset of cells as an equipotential group that is competent to respond to the sevenless developmental signal and another subset that confer proper photoreceptor identity by positively regulating the homeo box gene Bar. Involved in the aop/pnt dynamic in a Ras-dependent manner to regulate pros expression. Promotes apoptosis in the pupal eye by directly activating aos and klu. Also modulates hid- and rpr-mediated cell death. Regulates amos function in olfactory sensilla development. Ref.1 Ref.2 Ref.5 Ref.6
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed in the pupal eye during programmed cell death. Ref.2
Sequence similarities	Contains 1 Runt domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	R7 cell development Inferred from mutant phenotype. Source: FlyBase antennal development Traceable author statement. Source: FlyBase compound eye cone cell differentiation Inferred from mutant phenotype. Source: FlyBase embryonic crystal cell differentiation Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived female genitalia development Inferred from mutant phenotype. Source: FlyBase lymph gland crystal cell differentiation Traceable author statement. Source: FlyBase negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: FlyBase oogenesis Traceable author statement. Source: FlyBase positive regulation of compound eye retinal cell programmed cell death Inferred from mutant phenotype Ref.6. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.6. Source: UniProtKB scab formation Inferred from mutant phenotype. Source: FlyBase sensory organ precursor cell fate determination Traceable author statement. Source: FlyBase signal transduction Non-traceable author statement. Source: FlyBase spermathecum morphogenesis Traceable author statement. Source: FlyBase
Cellular component	nucleus Inferred by curator Ref.6. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay. Source: FlyBase sequence-specific distal enhancer binding RNA polymerase II transcription factor activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 826	826	Protein lozenge		PRO_0000174666
Regions
	Domain	275 – 403	129	Runt
	Compositional bias	58 – 105	48	Gly-rich
	Compositional bias	107 – 153	47	His/Pro-rich
	Compositional bias	211 – 263	53	Asn/Ser-rich
	Compositional bias	443 – 495	53	Ala/Gly-rich
	Compositional bias	549 – 566	18	His-rich
	Compositional bias	571 – 581	11	Pro-rich
	Compositional bias	585 – 668	84	Gly-rich
	Compositional bias	694 – 712	19	Pro-rich
	Compositional bias	763 – 793	31	Gln-rich
Experimental info
	Sequence conflict	8	1	A → V in AAC47196. Ref.1
	Sequence conflict	16	1	S → F in AAC47196. Ref.1
	Sequence conflict	252	1	N → NNNN Ref.1
	Sequence conflict	341 – 342	2	EL → DV in AAC47196. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9W349 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: BCDF558F3E42B5CC	FASTA	826	84,722
	10 20 30 40 50 60 MHLHLLAAEK TPPSPSPNPT PTPSASPSGH TGAAGESQLD LASQTESQLQ LGLPLASGYG 70 80 90 100 110 120 LGLGLGLGLG LGLGQELVAD HSTTVAPVSV AGPGRLGRSI NGSGGSHHHH HLHHHYSPYH 130 140 150 160 170 180 HAHPYHPPHP HAPHHHHHHH PPYPYPPAGP HPPAMVTSSS TSPTGNGWSS STGDFKGITA 190 200 210 220 230 240 VATATGGGVG GATQGATAST GATAAEVLAV SSSASVGSSS PTGGASNGTA HSGHSGHTGG 250 260 270 280 290 300 HSSSTASNNN NNGASNSNSN NNNAVHQDLL WMERLVQKRQ QEHPGELVRT SNPYFLCSAL 310 320 330 340 350 360 PAHWRSNKTL PMAFKVVALA EVGDGTYVTI RAGNDENCCA ELRNFTTQMK NDVAKFNDLR 370 380 390 400 410 420 FVGRSGRGKS FTLTITVATS PPQVATYAKA IKVTVDGPRE PRSKTSPTGG PHYRALGLGQ 430 440 450 460 470 480 RPYIDGFPST KALHELESLR RSAKVAAVTT AAAAAATAAS AANAVAAAAA AVAVTPTGGG 490 500 510 520 530 540 GGVAAGGVAG GAGAGLVQQL SSNYSSPNST INSDCQVYKP NAPHIQAAEM MGAGEWTNGS 550 560 570 580 590 600 SSSAAAYYHS HAHHPHAHHA HAHLQHQMAL PPPPPPPAAA PVSVGVGGNG ATMGMGMGVG 610 620 630 640 650 660 VGMGMNHYGG GYDSANSLEA GQYAAHLPAV LPEMHGHGFA TDPYQTAGYG GGNTGGGSAS 670 680 690 700 710 720 KSELDYGGSY NQAWSNGYQN YQYGSCLATA QYGPQAAPPP QPPPPPPVVL CPQLYSTVNQ 730 740 750 760 770 780 NQIHLHLHSS EKLEQYLGTA TSADHLTIGS LTGSSRSSIE IGQDQYHQQV HHAQQQQQQQ 790 800 810 820 QQQQQVHHPQ QQQVESAGEV GGSGAGGVES AREEDVGDLS QVWRPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Patterning of cells in the Drosophila eye by Lozenge, which shares homologous domains with AML1." Daga A., Karlovich C.A., Dumstrei K., Banerjee U. Genes Dev. 10:1194-1205(1996) [PubMed: 8675007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Yan regulates Lozenge during Drosophila eye development." Behan K.J., Nichols C.D., Cheung T.L., Farlow A., Hogan B.M., Batterham P., Pollock J.A. Dev. Genes Evol. 212:267-276(2002) [PubMed: 12111211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"Amos, a proneural gene for Drosophila olfactory sense organs that is regulated by lozenge." Goulding S.E., zur Lage P., Jarman A.P. Neuron 25:69-78(2000) [PubMed: 10707973] [Abstract] Cited for: FUNCTION.
[6]	"Lozenge directly activates argos and klumpfuss to regulate programmed cell death." Wildonger J., Sosinsky A., Honig B., Mann R.S. Genes Dev. 19:1034-1039(2005) [PubMed: 15879554] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47849 mRNA. Translation: AAC47196.2. AF217651 Genomic DNA. Translation: AAF35308.1. AE014298 Genomic DNA. Translation: AAF46486.2.
RefSeq	NP_511099.2. NM_078544.2.
UniGene	Dm.2929.
3D structure databases
ProteinModelPortal	Q9W349.
SMR	Q9W349. Positions 282-402.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9W349.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0071320; FBpp0071255; FBgn0002576.
GeneID	31883.
KEGG	dme:Dmel_CG1689.
NMPDR	fig|7227.3.peg.17140.
UCSC	CG1689-RA. d. melanogaster.
Organism-specific databases
CTD	17106.
FlyBase	FBgn0002576. lz.
Phylogenomic databases
eggNOG	inNOG09917.
GeneTree	EMGT00050000001331.
InParanoid	Q9W349.
OMA	HLHSSEK.
OrthoDB	EOG49P8G0.
PhylomeDB	Q9W349.
Gene expression databases
Bgee	Q9W349.
GermOnline	CG1689. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013524. AML1/Runt_N. IPR000040. AML1_Runt. IPR008967. p53-like_TF_DNA-bd. IPR012346. p53/RUNT-type_TF_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.60.40.720. p53_RUNT_DNA_bd. 1 hit.
KO	K09280.
PANTHER	PTHR11950. AML1_Runt. 1 hit.
Pfam	PF00853. Runt. 1 hit. [Graphical view]
PRINTS	PR00967. ONCOGENEAML1.
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS51062. RUNT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	775784.

Entry information

Entry name

LOZEN_DROME

Accession

Primary (citable) accession number: Q9W349
Secondary accession number(s): Q24183, Q9NHX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project