Q9W2N0 (CAPZA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-actin-capping protein subunit alpha

Gene names

Name:	cpa
ORF Names:	CG10540

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Ligand	Actin-binding
Molecular function	Actin capping
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin filament capping Inferred from electronic annotation. Source: UniProtKB-KW actin filament organization Inferred from mutant phenotype. Source: FlyBase germarium-derived oocyte fate determination Inferred from mutant phenotype. Source: FlyBase negative regulation of filopodium assembly Inferred from mutant phenotype. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase regulation of lamellipodium assembly Inferred from mutant phenotype. Source: FlyBase wing disc development Inferred from mutant phenotype. Source: FlyBase
Cellular component	F-actin capping protein complex Inferred from electronic annotation. Source: InterPro apical plasma membrane Inferred from direct assay. Source: FlyBase microtubule associated complex Inferred from direct assay. Source: FlyBase
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	F-actin-capping protein subunit alpha		PRO_0000208639

Sequences

	Sequence		Length	Mass (Da)
	Q9W2N0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8E52D9914EEDAE00	FASTA	286	32,759
	10 20 30 40 50 60 MEQTPITDAE KVRIVSDFIL HAPPGEFNEV FNDVRELLKN DTLLKDGASH AFAQYNKDQL 70 80 90 100 110 120 TPVRIEGTDH NAIISEHNDL GNGRFYDPRT KQAFKYDHLR KEASDYQDVE ADATAEPWRA 130 140 150 160 170 180 ALDLETLAYT ASHYRHGVSS VFGKAQGNQI TLTICIEDHQ FQPKNYWNGR WRSQWHVTFQ 190 200 210 220 230 240 AGSGTAELKG VLKVQVHYYE DGNVQLVSSK ECRESVVVSN EQQVAKEVIR LIEDAENEYQ 250 260 270 280 LAISENYQTM SDTTFKAMRR QLPITRTKID WSKIVSYSIG KELKTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013599 Genomic DNA. Translation: AAF46660.1. AY069084 mRNA. Translation: AAL39229.1.
RefSeq	NP_611539.1. NM_137695.3.
3D structure databases
ProteinModelPortal	Q9W2N0.
SMR	Q9W2N0. Positions 6-277.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18409N.
IntAct	Q9W2N0. 4 interactions.
MINT	MINT-857691.
STRING	Q9W2N0.
Proteomic databases
PRIDE	Q9W2N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0071532; FBpp0071461; FBgn0034577.
GeneID	37387.
KEGG	dme:Dmel_CG10540.
NMPDR	fig|7227.3.peg.6565.
Organism-specific databases
CTD	37387.
FlyBase	FBgn0034577. cpa.
Phylogenomic databases
eggNOG	inNOG08781.
GeneTree	EMGT00050000007618.
InParanoid	Q9W2N0.
OMA	AIESWRN.
OrthoDB	EOG4M9077.
PhylomeDB	Q9W2N0.
Gene expression databases
Bgee	Q9W2N0.
GermOnline	CG10540. Drosophila melanogaster.
Family and domain databases
InterPro	IPR017865. F-actin_cap_asu_CS. IPR002189. WASH_F-actin_cap_alpha. [Graphical view]
KO	K10364.
PANTHER	PTHR10653. F-actin_cap_A. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	803392.

Entry information

Entry name

CAPZA_DROME

Accession

Primary (citable) accession number: Q9W2N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project