Reviewed, UniProtKB/Swiss-Prot Q9W2N0 (CAPZA_DROME)

Names and origin

Protein names

Recommended name:
    F-actin-capping protein subunit alpha

Gene names

Name:	cpa
ORF Names:	CG10540

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Ligand	Actin-binding
Molecular function	Actin capping
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament organization Inferred from mutant phenotype. Source: FlyBase barbed-end actin filament capping Inferred from electronic annotation. Source: UniProtKB-KW phagocytosis, engulfment Inferred from mutant phenotype. Source: FlyBase regulation of lamellipodium assembly Inferred from mutant phenotype. Source: FlyBase wing disc development Inferred from mutant phenotype. Source: FlyBase
Cellular component	F-actin capping protein complex Inferred from electronic annotation. Source: InterPro apical plasma membrane Inferred from direct assay. Source: FlyBase
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VDM7	1	EBI-173404,EBI-128958
BG:DS08249.3	Q9VJW5	1	EBI-173404,EBI-145138
cpb	P48603	1	EBI-173404,EBI-95160

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	F-actin-capping protein subunit alpha		PRO_0000208639

Sequences

	Sequence		Length	Mass (Da)
	Q9W2N0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8E52D9914EEDAE00	FASTA	286	32,759
	10 20 30 40 50 60 MEQTPITDAE KVRIVSDFIL HAPPGEFNEV FNDVRELLKN DTLLKDGASH AFAQYNKDQL 70 80 90 100 110 120 TPVRIEGTDH NAIISEHNDL GNGRFYDPRT KQAFKYDHLR KEASDYQDVE ADATAEPWRA 130 140 150 160 170 180 ALDLETLAYT ASHYRHGVSS VFGKAQGNQI TLTICIEDHQ FQPKNYWNGR WRSQWHVTFQ 190 200 210 220 230 240 AGSGTAELKG VLKVQVHYYE DGNVQLVSSK ECRESVVVSN EQQVAKEVIR LIEDAENEYQ 250 260 270 280 LAISENYQTM SDTTFKAMRR QLPITRTKID WSKIVSYSIG KELKTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF46660.1. AY069084 mRNA. Translation: AAL39229.1.
RefSeq	NP_611539.1.
UniGene	Dm.2291
3D structure databases
HSSP	HSSP built from PDB template 1IZN based on UniProtKB P13127.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:18409N.
IntAct	Q9W2N0. 8 interactions.
Proteomic databases
PRIDE	Q9W2N0.
Genome annotation databases
Ensembl	FBgn0034577. Drosophila melanogaster. [Contig view]
GeneID	37387.
KEGG	dme:Dmel_CG10540.
NMPDR	fig|7227.3.peg.6565.
Organism-specific databases
FlyBase	FBgn0034577. cpa.
Phylogenomic databases
HOGENOM	Q9W2N0.
OMA	Q9W2N0. YDHLRKE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005740-MON.
Gene expression databases
ArrayExpress	Q9W2N0.
GermOnline	CG10540. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002189. F-actin_cap_asu. IPR018315. F-actin_cap_asu_actin_bd. IPR017865. F-actin_cap_asu_CS. [Graphical view]
PANTHER	PTHR10653. F-actin_cap_A. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
ProDom	PD006960. F-actin_cap_A. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	803392.

Entry information

Entry name

CAPZA_DROME

Accession

Primary (citable) accession number: Q9W2N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project