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Protein

Phenoloxidase 3

Gene

PPO3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinone (By similarity).By similarity

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi209 – 2091Copper ABy similarity
Metal bindingi213 – 2131Copper ABy similarity
Metal bindingi239 – 2391Copper ABy similarity
Metal bindingi366 – 3661Copper BBy similarity
Metal bindingi370 – 3701Copper BBy similarity
Metal bindingi406 – 4061Copper BBy similarity

GO - Molecular functioni

  1. dopamine monooxygenase activity Source: FlyBase
  2. L-DOPA monooxygenase activity Source: FlyBase
  3. metal ion binding Source: UniProtKB-KW
  4. monophenol monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. dopamine metabolic process Source: FlyBase
  2. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenoloxidase 3 (EC:1.14.18.1)
Alternative name(s):
Diphenol oxidase A3
Diphenoloxidase subunit A3
Prophenoloxidase 59
Tyrosinase A3
Gene namesi
Name:PPO3
Synonyms:Dox-3, Dox-A3, proPO59
ORF Names:CG42640
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0261363. PPO3.

Subcellular locationi

Secreted By similarity
Note: Expression not detected in larval hemolymph.1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 4848By similarityPRO_0000035905Add
BLAST
Chaini51 – 683633Phenoloxidase 3PRO_0000035906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi574 ↔ 617By similarity
Disulfide bondi576 ↔ 624By similarity

Post-translational modificationi

Upon activation, a trypsin type protease cleaves prophenol oxidase to yield the active enzyme.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9W1V6.
PRIDEiQ9W1V6.

Expressioni

Developmental stagei

Expression is very low during all stages of development.1 Publication

Gene expression databases

ExpressionAtlasiQ9W1V6. differential.

Structurei

3D structure databases

ProteinModelPortaliQ9W1V6.
SMRiQ9W1V6. Positions 4-673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Phylogenomic databases

eggNOGiNOG297156.
GeneTreeiENSGT00730000111961.
InParanoidiQ9W1V6.
KOiK00505.
OMAiWRERIYE.
OrthoDBiEOG7XPZ54.
PhylomeDBiQ9W1V6.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9W1V6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKNLLLL FDHPTEPVFM DKGGNGTVFD VPDSYVTDRY NQMCKKVQRR
60 70 80 90 100
VSSASEKNVQ VKEIAIPDLS CSMRLGRSEQ FSIFLKSHRK MASHLIEIFT
110 120 130 140 150
KMQTVDELQS VAVYARDRVN PVLFNYALSV ALLHRPDTKD LELPAFAQTF
160 170 180 190 200
PDRFIDSKML RSMREESFVV ERSAARLPVV SSVKYTASDL DVEHRLWYFR
210 220 230 240 250
EDLGVNLHHW HWHLVYPIEA PDRSIVDKDR RGELFYYMHQ QIIARYNAER
260 270 280 290 300
LSNHMARVQP FNNLDEPIAE GYFPKMDSLV ASRAYPPRFD NTRLSDVDRP
310 320 330 340 350
NNQLRVGIDD MKRWRERIYE AIHQGYVLDT NNEKIVLDDA KGIDILGNII
360 370 380 390 400
EASDLTPNST LYGDFHNMGH ILIAYSHDPT NKHLEYAGVM GDASTAMRDP
410 420 430 440 450
IFYKWHAFID NMFQEHKRLL SPYEKQELSF PDVRVESIQV ESQGQVNRLT
460 470 480 490 500
TFWQESDVDM SRGLDFVPRG HVLARFTHLQ HHEFSYTIKV ENSSEATRYG
510 520 530 540 550
YVRIFLAPKL DDRNAPMLLE QQRLMMVELD KFVVTMPPGS HTITRNSTES
560 570 580 590 600
SVTIPFERTF RNLDKLEELQ NFLCGCGWPQ HMLIPKGRPE GLRFELFVMV
610 620 630 640 650
SNYEEDKVDQ TVADCGCSIA ASYCGLRDRL YPDRKSMGFP FDRKPRRGSE
660 670 680
ILENFLTPNM CAVEVIITHE DRTEKLREVP ARS
Length:683
Mass (Da):79,314
Last modified:May 1, 2000 - v1
Checksum:i437CBDD9E8A278BF
GO

Sequence cautioni

The sequence BAB43866.1 differs from that shown.Chimeric cDNA. It is a chimera between Dox-A3 and CG8193.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461Y → N in AAL29172 (PubMed:12537569).Curated
Sequence conflicti491 – 4911E → D in AAL29172 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF46946.1.
AY061624 mRNA. Translation: AAL29172.1.
AB055857 Genomic DNA. Translation: BAB43866.1. Sequence problems.
RefSeqiNP_524760.1. NM_080021.4.

Genome annotation databases

EnsemblMetazoaiFBtr0302290; FBpp0291496; FBgn0261363.
GeneIDi44513.
KEGGidme:Dmel_CG42640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF46946.1.
AY061624 mRNA. Translation: AAL29172.1.
AB055857 Genomic DNA. Translation: BAB43866.1. Sequence problems.
RefSeqiNP_524760.1. NM_080021.4.

3D structure databases

ProteinModelPortaliQ9W1V6.
SMRiQ9W1V6. Positions 4-673.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9W1V6.
PRIDEiQ9W1V6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0302290; FBpp0291496; FBgn0261363.
GeneIDi44513.
KEGGidme:Dmel_CG42640.

Organism-specific databases

CTDi44513.
FlyBaseiFBgn0261363. PPO3.

Phylogenomic databases

eggNOGiNOG297156.
GeneTreeiENSGT00730000111961.
InParanoidiQ9W1V6.
KOiK00505.
OMAiWRERIYE.
OrthoDBiEOG7XPZ54.
PhylomeDBiQ9W1V6.

Miscellaneous databases

GenomeRNAii44513.
NextBioi795776.

Gene expression databases

ExpressionAtlasiQ9W1V6. differential.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PANTHERiPTHR11511. PTHR11511. 1 hit.
PfamiPF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]
PRINTSiPR00187. HAEMOCYANIN.
SUPFAMiSSF48050. SSF48050. 1 hit.
SSF48056. SSF48056. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence."
    Asada N., Yokoyama G., Kawamoto N., Norioka S., Hatta T.
    Biochem. Genet. 41:151-163(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-664, PROTEOLYTIC CLEAVAGE.
    Strain: Oregon-R.
    Tissue: Larva and Pupae.
  5. "Identification of the gene encoding pro-phenoloxidase A(3) in the fruitfly, Drosophila melanogaster."
    Asano T., Takebuchi K.
    Insect Mol. Biol. 18:223-232(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPPO3_DROME
AccessioniPrimary (citable) accession number: Q9W1V6
Secondary accession number(s): Q95R43, Q9BLD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.