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Protein

Phosphatidylinositol 3-kinase

Gene

Pi3K59F

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.UniRule annotation

GO - Molecular functioni

  • 1-phosphatidylinositol-3-kinase activity Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • phosphatidylinositol 3-kinase activity Source: FlyBase

GO - Biological processi

  • autophagy Source: FlyBase
  • cellular response to starvation Source: FlyBase
  • dsRNA transport Source: FlyBase
  • endocytosis Source: FlyBase
  • larval midgut cell programmed cell death Source: FlyBase
  • neuron remodeling Source: FlyBase
  • phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  • phosphatidylinositol-mediated signaling Source: InterPro
  • phosphatidylinositol phosphorylation Source: FlyBase
  • positive regulation of nurse cell apoptotic process Source: FlyBase
  • regulation of autophagy Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-109704. PI3K Cascade.
R-DME-1632852. Macroautophagy.
R-DME-1660514. Synthesis of PIPs at the Golgi membrane.
R-DME-1660516. Synthesis of PIPs at the early endosome membrane.
R-DME-1660517. Synthesis of PIPs at the late endosome membrane.
R-DME-168138. Toll Like Receptor 9 (TLR9) Cascade.
R-DME-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinaseUniRule annotation (EC:2.7.1.137UniRule annotation)
Gene namesi
Name:Pi3K59FImported
ORF Names:CG5373Imported, Dmel_CG5373Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0015277. Pi3K59F.

Subcellular locationi

GO - Cellular componenti

  • autophagosome membrane Source: FlyBase
  • early endosome Source: FlyBase
  • phosphatidylinositol 3-kinase complex, class III Source: FlyBase
  • phosphatidylinositol 3-kinase complex, class III, type I Source: FlyBase
Complete GO annotation...

Interactioni

Protein-protein interaction databases

IntActiQ9W1M7. 8 interactions.
MINTiMINT-780114.
STRINGi7227.FBpp0071973.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X6FX-ray3.30A/B258-949[»]
2X6HX-ray2.90A/B258-949[»]
2X6IX-ray3.40A/B258-949[»]
2X6JX-ray3.50A/B258-949[»]
2X6KX-ray3.50A/B258-949[»]
SMRiQ9W1M7. Positions 253-420, 530-948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 190155C2 PI3K-typeInterPro annotationAdd
BLAST
Domaini283 – 579297PIK helicalInterPro annotationAdd
BLAST
Domaini693 – 947255PI3K/PI4KInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.UniRule annotationSAAS annotation
Contains PI3K/PI4K domain.SAAS annotation
Contains PIK helical domain.SAAS annotation

Phylogenomic databases

eggNOGiKOG0906. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00760000119110.
KOiK00914.
OMAiLAITVWD.
PhylomeDBiQ9W1M7.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 2 hits.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 3 hits.
PfamiPF00454. PI3_PI4_kinase. 2 hits.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W1M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQPDDHFRY IHSSSLHERV QIKVGTLEGK KRQPDYEKLL EDPILRFSGL
60 70 80 90 100
YSEEHPSFQV RLQVFNQGRP YCLPVTSSYK AFGKRWSWNE WVTLPLQFSD
110 120 130 140 150
LPRSAMLVLT ILDCSGAGQT TVIGGTSISM FGKDGMFRQG MYDLRVWLGV
160 170 180 190 200
EGDGNFPSRT PGKGKESSKS QMQRLGKLAK KHRNGQVQKV DWLDRLTFRE
210 220 230 240 250
IEVINEREKR MSDYMFLMIE FPAIVVDDMY NYAVVYFEPE GDVKYKLPAK
260 270 280 290 300
PKLVSVPDSE IQMENLVERK HHRLARSERS GISDRDAKPT ASIRDQLHTI
310 320 330 340 350
VYRYPPTYVL SSEEQDLVWK FRFYLSSHKK ALTKFLKCIN WKLEDEVTQA
360 370 380 390 400
LWMLANWAPM DVEDALELLS PTFTHPQVRK YAVSRLAQAP DEDLLLYLLQ
410 420 430 440 450
LVQALKYEDP RHIVHLHGCI FPERDVVRSI LDDNGSLLDQ SSLSDLSATS
460 470 480 490 500
SGLHGSVIPA NQRAASVLAA IKSDKSVSPG SAGGSGSGGQ GSVALPNPSA
510 520 530 540 550
PATPGSSSLP CDSNSNALML AEGISFGSVP ANLCTFLIQR ACTNATLANY
560 570 580 590 600
FYWYLSIEVE EVESVRKQDE RAHDMYAMVL KMFLKVLENG NFNLRGIFYN
610 620 630 640 650
LRKQRRFIDE LVKLVKLVAK EPGNRNKKTE KFQKLLAEQD MFKVNFTNFE
660 670 680 690 700
PIPFPLDPEI YITKIVPMRT SLFKSALMPA KLTFVTSIAH HEYAAIFKHG
710 720 730 740 750
DDLRQDQLIL QMITLMDKLL RRENLDLKLT PYKVLATSSK HGFLQYVDSC
760 770 780 790 800
TVAEVLAREG NIHNFFRKHH PCDNGPYGIS AEVMDTYIKS CAGYCVITYL
810 820 830 840 850
LGVGDRHLDN LLLTTNGKLF HIDFGYILGR DPKPMPPPMK LSKEMVEAMG
860 870 880 890 900
GISSEHHHEF RKQCYTAYLH LRRHANVMLN LFSLMVDATV PDIALEPDKA
910 920 930 940
VKKVEENLQL GLTDEEAVQH LQSLLDVSIT AVMPALVEQI HRFTQYWRK
Length:949
Mass (Da):107,889
Last modified:October 1, 2002 - v2
Checksum:i354B96CC09A3F3A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47030.2.
AY058362 mRNA. Translation: AAL13591.1.
RefSeqiNP_477133.1. NM_057785.5.
UniGeneiDm.1676.

Genome annotation databases

EnsemblMetazoaiFBtr0072064; FBpp0071973; FBgn0015277.
GeneIDi37733.
KEGGidme:Dmel_CG5373.
UCSCiCG5373-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47030.2.
AY058362 mRNA. Translation: AAL13591.1.
RefSeqiNP_477133.1. NM_057785.5.
UniGeneiDm.1676.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X6FX-ray3.30A/B258-949[»]
2X6HX-ray2.90A/B258-949[»]
2X6IX-ray3.40A/B258-949[»]
2X6JX-ray3.50A/B258-949[»]
2X6KX-ray3.50A/B258-949[»]
SMRiQ9W1M7. Positions 253-420, 530-948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9W1M7. 8 interactions.
MINTiMINT-780114.
STRINGi7227.FBpp0071973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072064; FBpp0071973; FBgn0015277.
GeneIDi37733.
KEGGidme:Dmel_CG5373.
UCSCiCG5373-RA. d. melanogaster.

Organism-specific databases

CTDi37733.
FlyBaseiFBgn0015277. Pi3K59F.

Phylogenomic databases

eggNOGiKOG0906. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00760000119110.
KOiK00914.
OMAiLAITVWD.
PhylomeDBiQ9W1M7.

Enzyme and pathway databases

ReactomeiR-DME-109704. PI3K Cascade.
R-DME-1632852. Macroautophagy.
R-DME-1660514. Synthesis of PIPs at the Golgi membrane.
R-DME-1660516. Synthesis of PIPs at the early endosome membrane.
R-DME-1660517. Synthesis of PIPs at the late endosome membrane.
R-DME-168138. Toll Like Receptor 9 (TLR9) Cascade.
R-DME-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

GenomeRNAii37733.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 2 hits.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 3 hits.
PfamiPF00454. PI3_PI4_kinase. 2 hits.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BerkeleyImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BerkeleyImported.
  5. "The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective."
    Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., Celniker S.E.
    Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  6. "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."
    Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., Karpen G.H.
    Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  7. "Combined evidence annotation of transposable elements in genome sequences."
    Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., Ashburner M., Anxolabehere D.
    PLoS Comput. Biol. 1:166-175(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  8. Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S., Svirskas R., Rubin G.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  9. "Drosophila melanogaster release 4 sequence."
    Berkeley Drosophila Genome Project
    Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R., Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C., Rubin G.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  10. "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."
    Smith C.D., Shu S., Mungall C.J., Karpen G.H.
    Science 316:1586-1591(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  11. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  12. "Shaping development of autophagy inhibitors with the structure of the lipid kinase Vps34."
    Miller S., Tavshanjian B., Oleksy A., Perisic O., Houseman B.T., Shokat K.M., Williams R.L.
    Science 327:1638-1642(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 258-949.
  13. FlyBase
    Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiQ9W1M7_DROME
AccessioniPrimary (citable) accession number: Q9W1M7
Secondary accession number(s): Q95U22
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 2000
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.