Q9W1K5 (SESN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sestrin homolog

Gene names

Name:	Sesn
ORF Names:	CG11299

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the sestrin family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Inferred from electronic annotation. Source: InterPro inter-male aggressive behavior Inferred from mutant phenotype PubMed 19519879. Source: FlyBase mitochondrion degradation Inferred from mutant phenotype PubMed 20203043. Source: FlyBase multicellular organismal aging Inferred from mutant phenotype PubMed 20203043. Source: FlyBase negative regulation of cell growth Inferred from mutant phenotype PubMed 20203043. Source: FlyBase regulation of reactive oxygen species metabolic process Inferred from mutant phenotype PubMed 20203043. Source: FlyBase
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cyp4s3	Q9VXY0	1	EBI-212765,EBI-133844
Flo	O61491	1	EBI-212765,EBI-212813
l(2)k10201	Q9V574	1	EBI-212765,EBI-154227
Pp2B-14D	Q27889	1	EBI-212765,EBI-132663
tna	Q9VTB8	1	EBI-212765,EBI-162797

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9W1K5-1)

Also known as: A; B; C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9W1K5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     80-111: AAARHQCPYLVKRYEKEFINQGGDSAWLGGLD → SIFRLLFPTFISLPNWPKTIAIDWANEEHGLL
     112-497: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9W1K5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     433-437: LLVRP → VRNQL
     438-497: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Sestrin homolog		PRO_0000221186
Amino acid modifications
	Modified residue	185	1	Phosphoserine Ref.4
	Modified residue	190	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	80 – 111	32	AAARH…LGGLD → SIFRLLFPTFISLPNWPKTI AIDWANEEHGLL in isoform 2.		VSP_006065
	Alternative sequence	112 – 497	386	Missing in isoform 2.		VSP_006066
	Alternative sequence	433 – 437	5	LLVRP → VRNQL in isoform 3.		VSP_006067
	Alternative sequence	438 – 497	60	Missing in isoform 3.		VSP_006068

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) (B) (C) [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 39AC03B8F653CA30	FASTA	497	55,853
	10 20 30 40 50 60 MYYAVDYYAD MGQISQDCFA ATQTGASDFD MDELDDLDQV TQVIGYHPQF HDHFLATQNF 70 80 90 100 110 120 IMKGDGPLPN DYRYYLAIIA AARHQCPYLV KRYEKEFINQ GGDSAWLGGL DFIPAKLRAI 130 140 150 160 170 180 YDINKILAHR PWLLRKEHIE RLTKGKNSWS LSEVVHAMVL LSHFHSLSSF VFSCGLTQKL 190 200 210 220 230 240 DGLSSPKLKS PPAAVAALAP TILITPTSPT EPQKGKPVLA EISLNNANPD YDSQTAASSN 250 260 270 280 290 300 GGAPPDSANA VADGPDATTL NGYLATAQQL PQQHGISVET LMERMKVLSQ KQDECSEAEL 310 320 330 340 350 360 SSRFQKVEQQ TAELAAVTPE AAVGVPTNLS HYVDDANFIY QDFARRGTES INTFRIQDYS 370 380 390 400 410 420 WEDHGYSLVD GLYNDVGIFL DAKFRAAYNL TYCTMGGIKN VDTSKFRRAI WNYIQCIYGI 430 440 450 460 470 480 RHDDYDYGEV NQLLVRPLKM FIKTACCFPE RITTKDYDSV LVELQDSEKV HVNLMIMEAR 490 NQAELLYALR EIMRYMT
	Isoform 2 [UniParc]. Checksum: 4C254D2829C4F254	FASTA	111	12,822
	10 20 30 40 50 60 MYYAVDYYAD MGQISQDCFA ATQTGASDFD MDELDDLDQV TQVIGYHPQF HDHFLATQNF 70 80 90 100 110 IMKGDGPLPN DYRYYLAIIS IFRLLFPTFI SLPNWPKTIA IDWANEEHGL L
	Isoform 3 [UniParc]. Checksum: 3BDF1D7E5125004A	FASTA	437	48,759
	10 20 30 40 50 60 MYYAVDYYAD MGQISQDCFA ATQTGASDFD MDELDDLDQV TQVIGYHPQF HDHFLATQNF 70 80 90 100 110 120 IMKGDGPLPN DYRYYLAIIA AARHQCPYLV KRYEKEFINQ GGDSAWLGGL DFIPAKLRAI 130 140 150 160 170 180 YDINKILAHR PWLLRKEHIE RLTKGKNSWS LSEVVHAMVL LSHFHSLSSF VFSCGLTQKL 190 200 210 220 230 240 DGLSSPKLKS PPAAVAALAP TILITPTSPT EPQKGKPVLA EISLNNANPD YDSQTAASSN 250 260 270 280 290 300 GGAPPDSANA VADGPDATTL NGYLATAQQL PQQHGISVET LMERMKVLSQ KQDECSEAEL 310 320 330 340 350 360 SSRFQKVEQQ TAELAAVTPE AAVGVPTNLS HYVDDANFIY QDFARRGTES INTFRIQDYS 370 380 390 400 410 420 WEDHGYSLVD GLYNDVGIFL DAKFRAAYNL TYCTMGGIKN VDTSKFRRAI WNYIQCIYGI 430 RHDDYDYGEV NQVRNQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Berkeley. Tissue: Embryo.
[4]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-190, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013599 Genomic DNA. Translation: AAF47053.2. AE013599 Genomic DNA. Translation: ABV53897.1. AE013599 Genomic DNA. Translation: ABV53898.1. AY069626 mRNA. Translation: AAL39771.1.
RefSeq	NP_001097437.1. NM_001103967.1. NP_001097438.1. NM_001103968.1. NP_611821.1. NM_137977.3.
UniGene	Dm.2037.
3D structure databases
ProteinModelPortal	Q9W1K5.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19601N.
IntAct	Q9W1K5. 5 interactions.
MINT	MINT-343891.
Proteomic databases
PaxDb	Q9W1K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072087; FBpp0071996; FBgn0034897. FBtr0113113; FBpp0112026; FBgn0034897. FBtr0113114; FBpp0112027; FBgn0034897.
GeneID	37755.
KEGG	dme:Dmel_CG11299.
UCSC	CG11299-RA. d. melanogaster.
Organism-specific databases
CTD	37755.
FlyBase	FBgn0034897. Sesn.
Phylogenomic databases
eggNOG	NOG312456.
GeneTree	ENSGT00440000040103.
InParanoid	Q9W1K5.
KO	K10141.
OMA	WIISSSS.
OrthoDB	EOG4VQ84M.
PhylomeDB	Q9W1K5.
Gene expression databases
Bgee	Q9W1K5.
GermOnline	CG11299. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006730. PA26. [Graphical view]
PANTHER	PTHR12474. PTHR12474. 1 hit.
Pfam	PF04636. PA26. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	37755.
NextBio	805253.

Entry information

Entry name

SESN_DROME

Accession

Primary (citable) accession number: Q9W1K5
Secondary accession number(s): A8DYN7, Q9I7V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project