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Protein

DNA ligase 1

Gene

DNA-ligI

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei395N6-AMP-lysine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • DNA ligase (ATP) activity Source: FlyBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DME-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-69183. Processive synthesis on the lagging strand.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1PROSITE-ProRule annotation)
Alternative name(s):
DNA ligase I
Short name:
DmDNA Lig I
Gene namesi
ORF Names:CG5602
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0262619. DNA-ligI.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • mitochondrion Source: GO_Central
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003726571 – 747DNA ligase 1Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Phosphoserine1 Publication1
Modified residuei20Phosphoserine1 Publication1
Modified residuei42Phosphoserine1 Publication1
Modified residuei44Phosphoserine1 Publication1
Modified residuei46Phosphoserine1 Publication1
Modified residuei60Phosphoserine1 Publication1
Modified residuei65Phosphoserine2 Publications1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9W1H4.
PRIDEiQ9W1H4.

PTM databases

iPTMnetiQ9W1H4.

Expressioni

Gene expression databases

BgeeiFBgn0262619.
GenevisibleiQ9W1H4. DM.

Interactioni

Protein-protein interaction databases

BioGridi63384. 2 interactors.
IntActiQ9W1H4. 1 interactor.
MINTiMINT-920323.
STRINGi7227.FBpp0072041.

Structurei

3D structure databases

ProteinModelPortaliQ9W1H4.
SMRiQ9W1H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00860000133792.
InParanoidiQ9W1H4.
KOiK10747.
OMAiSQCPNYD.
OrthoDBiEOG091G06OS.
PhylomeDBiQ9W1H4.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W1H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKSITSFFK KKSDATDSPS PPKKVPKIDA KTELPDEPHI KSESASPETK
60 70 80 90 100
PKVEPMSVDS EEKTSPVKNV KKEPKEVDDK TTDKKVTTIG LNSTAATKED
110 120 130 140 150
VENYDPSADS YHPLKNAYWK DKKVTPYLAL ARTFQVIEET KGRLKMIDTL
160 170 180 190 200
SNFFCSVMLV SPEDLVPSVY LSINQLAPAY EGLELGVAET TLMKAICKAT
210 220 230 240 250
GRNLAHIKSQ TQLTGDLGIV AEQSRVSQRM MFQPAPLNVR DVFRKLREIA
260 270 280 290 300
KLSGQSKMDL VYNMFVACRS SEARFFIRSL IGKLRIGIAE QSLLTALAIG
310 320 330 340 350
LVKKNHIDDC KASKVPDVYK DEIVDTTLLL KTAYCQCPNY DIIIPAILKY
360 370 380 390 400
DIKELQERCP MHPGMPLRPM LAQPTKGVHE VFERFGGMQI TCEWKYDGER
410 420 430 440 450
AQIHRNEKGE ISIFSRNSEN NTAKYPDLIA RSTALLKGDV KSYIIDSEIV
460 470 480 490 500
AWDVERKQIL PFQVLSTRKR KNVDIEEIKV QVCVYIFDLL YINGTALVTK
510 520 530 540 550
NLSERRKLLL EHFQEVEGEW KFATALDTND IDEVQQFLEE SIKGNCEGLM
560 570 580 590 600
VKTLDEEATY EIAKRSRNWL KLKKDYLSNV GDSLDLVVIG GYKGKGRRTG
610 620 630 640 650
TYGGFLLACY DTENEEYQSI CKIGTGFTDE DLQTHSEFLG KHVTSAAKSY
660 670 680 690 700
YRYDPSLEPD HWFEPVQVWE VKCADLSLSP IHRAAIGIVD GERGISLRFP
710 720 730 740
RFIRIRDDKN SENATDANQV AHMYQSQDQV KNNQKSSTQM EMEDEFY
Length:747
Mass (Da):84,719
Last modified:October 1, 2002 - v2
Checksum:i9203F51CFFE3EF1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361 – 387Missing in AAK93363 (PubMed:12537569).CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47090.3.
AY051939 mRNA. Translation: AAK93363.1.
BT016103 mRNA. Translation: AAV36988.1.
RefSeqiNP_611843.2. NM_137999.3.
UniGeneiDm.19278.

Genome annotation databases

EnsemblMetazoaiFBtr0072132; FBpp0072041; FBgn0262619.
GeneIDi37791.
KEGGidme:Dmel_CG5602.
UCSCiCG5602-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47090.3.
AY051939 mRNA. Translation: AAK93363.1.
BT016103 mRNA. Translation: AAV36988.1.
RefSeqiNP_611843.2. NM_137999.3.
UniGeneiDm.19278.

3D structure databases

ProteinModelPortaliQ9W1H4.
SMRiQ9W1H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63384. 2 interactors.
IntActiQ9W1H4. 1 interactor.
MINTiMINT-920323.
STRINGi7227.FBpp0072041.

PTM databases

iPTMnetiQ9W1H4.

Proteomic databases

PaxDbiQ9W1H4.
PRIDEiQ9W1H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072132; FBpp0072041; FBgn0262619.
GeneIDi37791.
KEGGidme:Dmel_CG5602.
UCSCiCG5602-RA. d. melanogaster.

Organism-specific databases

CTDi37791.
FlyBaseiFBgn0262619. DNA-ligI.

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00860000133792.
InParanoidiQ9W1H4.
KOiK10747.
OMAiSQCPNYD.
OrthoDBiEOG091G06OS.
PhylomeDBiQ9W1H4.

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-DME-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
R-DME-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-DME-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DME-69183. Processive synthesis on the lagging strand.

Miscellaneous databases

GenomeRNAii37791.
PROiQ9W1H4.

Gene expression databases

BgeeiFBgn0262619.
GenevisibleiQ9W1H4. DM.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI1_DROME
AccessioniPrimary (citable) accession number: Q9W1H4
Secondary accession number(s): Q960P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.