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Protein

Sphingomyelin phosphodiesterase

Gene

CG3376

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide.UniRule annotation

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi277Zinc 1UniRule annotation1
Metal bindingi279Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi350Zinc 1UniRule annotation1
Metal bindingi350Zinc 2UniRule annotation1
Metal bindingi390Zinc 2UniRule annotation1
Metal bindingi498Zinc 2; via tele nitrogenUniRule annotation1
Metal bindingi532Zinc 2; via pros nitrogenUniRule annotation1
Metal bindingi534Zinc 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
LigandMetal-bindingUniRule annotation, ZincUniRule annotation

Enzyme and pathway databases

ReactomeiR-DME-1660662. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesteraseUniRule annotation (EC:3.1.4.12UniRule annotation)
Gene namesi
ORF Names:CG3376Imported, Dmel_CG3376Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034997. CG3376.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 46HelicalSequence analysisAdd BLAST24

Keywords - Cellular componenti

Membrane

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi158 ↔ 234UniRule annotation
Disulfide bondi161 ↔ 226UniRule annotation
Disulfide bondi189 ↔ 200UniRule annotation
Disulfide bondi292 ↔ 297UniRule annotation
Disulfide bondi298 ↔ 321UniRule annotation
Disulfide bondi458 ↔ 506UniRule annotation
Disulfide bondi658 ↔ 662UniRule annotation
Disulfide bondi668 ↔ 682UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Expressioni

Gene expression databases

BgeeiFBgn0034997.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini154 – 238Saposin B-typeInterPro annotationAdd BLAST85

Sequence similaritiesi

Belongs to the acid sphingomyelinase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiKOG3770. Eukaryota.
ENOG410YYPB. LUCA.
GeneTreeiENSGT00530000063095.
OrthoDBiEOG091G03M6.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
IPR011160. Sphingomy_PDE.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PIRSFiPIRSF000948. Sphingomy_PDE. 1 hit.
SMARTiView protein in SMART
SM00741. SapB. 1 hit.
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS50015. SAP_B. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9W188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQQPPWPS PISSSSSNPR GKCWLILVLM AVLPLVADVG ALPFLFTHIK
60 70 80 90 100
PQINWTQPEM ESWVIEEQPR SIEPHLEPVL HPNRTHRALS SDSTDAEFLQ
110 120 130 140 150
RLVSIRHNQT ASSRMLWYDV AGGDGLVYPP FVDKALKLLN LKQVAFEIEN
160 170 180 190 200
SVMSKVTCTA CRAGAGMLQH QIQSGKTDAE LIRMITDYCT NLNIQSARVC
210 220 230 240 250
QGVAQLFGSE LIYVLKRVNL SPDELCSFVI GDGCADVYNP YHEWEVIFPP
260 270 280 290 300
VPKPPRLPDL PIPMEAAPFF KVLHISDTHY DPHYAEGSNA DCNEPLCCRL
310 320 330 340 350
SSGRPATPNA AAGKWGDYRK CDTPKRTVDH MLSHIAETHK DIDYILWTGD
360 370 380 390 400
LPPHDVWNQT KEENLAIIKD TVKQMVEMFP GVPIFPALGN HESAPVNSFP
410 420 430 440 450
PPYVNQVDIS ISWLYDELDI QWRRWLPQSV THTVRRGAFY SVLVRPGFRI
460 470 480 490 500
ISLNMNYCNN KNWWLLLNST DPATELQWFI YELQSAEFSN EKVHVIGHIP
510 520 530 540 550
PGHSDCLKVW SRNFYKIISR YESTVTAQFY GHTHYDEFEM FYDPHDLNHP
560 570 580 590 600
NGIAYIGPSV SPYYDLNPGY RIYYVDGDHD ATTRLVIDHE SWIMNLKEAN
610 620 630 640 650
LYGYPIWYKL YTARAAYNMK ALRPSDWNNL LNELTNNQEL FELYYKYYWK
660 670 680 690 700
NSPARPTCDA ECKKRLICDC RSGRSHDRKH FCAEVESKID EESSKSWKSW
710 720 730
FYKGLTNSAE DRTEEPAPTT DGYGPFDVVI VNVGG
Length:735
Mass (Da):84,183
Last modified:May 1, 2000 - v1
Checksum:iA5078434669378F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47189.1.
AY061466 mRNA. Translation: AAL29014.1.
RefSeqiNP_611904.1. NM_138060.3.
UniGeneiDm.4051.

Genome annotation databases

EnsemblMetazoaiFBtr0072266; FBpp0072173; FBgn0034997.
FBtr0308245; FBpp0300565; FBgn0034997.
GeneIDi37884.
UCSCiCG3376-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiQ9W188_DROME
AccessioniPrimary (citable) accession number: Q9W188
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2000
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported