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Protein

Breast cancer type 2 susceptibility protein homolog

Gene

Brca2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in and required for double-strand break repair by meiotic and mitotic homologous recombination. During meiosis, has a dual role in the repair of meiotic double-stranded breaks and the efficient activation of the meiotic recombination checkpoint.2 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: FlyBase
  • DNA repair Source: FlyBase
  • DNA synthesis involved in double-strand break repair via homologous recombination Source: UniProtKB
  • double-strand break repair via homologous recombination Source: FlyBase
  • meiotic DNA repair synthesis involved in reciprocal meiotic recombination Source: UniProtKB
  • meiotic recombination checkpoint Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • response to camptothecin Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer type 2 susceptibility protein homolog1 Publication
Short name:
dmbrca21 Publication
Gene namesi
Name:Brca2Imported
ORF Names:CG30169
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0050169. Brca2.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Brca2 and spn-A/Rad51 become recruited to nuclear foci after DNA damage.1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Induced sensitivity to DNA damage induced by irradiation or treatment with hydroxyurea/ Repair by homologous recombination is dramatically decreased. Instead, large flanking deletions are formed, and repair by the non-conservative single-strand annealing pathway predominates.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971Breast cancer type 2 susceptibility protein homologPRO_0000392916Add
BLAST

Proteomic databases

PaxDbiQ9W157.
PRIDEiQ9W157.

Expressioni

Gene expression databases

BgeeiQ9W157.
GenevisibleiQ9W157. DM.

Interactioni

Subunit structurei

Interacts with Rad9 (PubMed:18266476). Interacts with spn-A/Rad51 (PubMed:17822964). Interacts with cyclin CycG (PubMed:22976300).3 Publications

Protein-protein interaction databases

BioGridi63488. 7 interactions.
IntActiQ9W157. 8 interactions.
MINTiMINT-1015104.
STRINGi7227.FBpp0072209.

Structurei

3D structure databases

ProteinModelPortaliQ9W157.
SMRiQ9W157. Positions 466-496, 572-603, 674-703, 749-779.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati570 – 60435BRCA2 1Sequence analysisAdd
BLAST
Repeati671 – 70535BRCA2 2Sequence analysisAdd
BLAST
Repeati746 – 78035BRCA2 3Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 3 BRCA2 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410J3H8. Eukaryota.
ENOG410ZXUJ. LUCA.
InParanoidiQ9W157.
OMAiQWPGMDV.
OrthoDBiEOG7GTT2Z.
PhylomeDBiQ9W157.

Family and domain databases

InterProiIPR015525. BRCA2.
IPR002093. BRCA2_repeat.
[Graphical view]
PANTHERiPTHR11289. PTHR11289. 1 hit.
PROSITEiPS50138. BRCA2_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQNGASGSH PNRLSQGRGA HARERGATVS AAANRSNIID EMAKICEADR
60 70 80 90 100
QTFAIARRTR GHERLAVDNS DFVAVEDLIL SYAEPTPEDQ VEMIMSDFCS
110 120 130 140 150
SPTYAEDEDE PSHESEPWFR FRNKRIRTYS RKRDPKSHKA VQNEKRRGSS
160 170 180 190 200
GLSVQRDLNT SFTSMACDFD ASSQKIHEVL LNLSQYFSAT ATASGPTPVP
210 220 230 240 250
SQIDLPTEAR QDSGDECFNA ELENLRDDLL QSGFTFEASF YENEHEQEGP
260 270 280 290 300
GTMADTIYGD SMQSVPTTRL RLESGKRNVW SDADSTLKKA DVEIAENKKL
310 320 330 340 350
LMGQTQAKNV DIEENTNFIL EGIPLSEWLT PMELPEISKD VIKHIPDKKL
360 370 380 390 400
KLEPSSQKEQ KSSKDSNASK VRGASKQSCD INTKNEGTTI LDQPNAAEQE
410 420 430 440 450
NLLNDGELLE EFLFNDWQPM QCSNGPSTSK NAIQGPKENI NSINLDDKEQ
460 470 480 490 500
PEKQTPNKSQ TISSHQLNGI RKKSFKFIEV SEEMKIKGEE FVDKVVSGLY
510 520 530 540 550
HFSHKCNLRT EEYSDNHSQV MESTQCAEFK SAPSKPIEIL DGKETYNAIA
560 570 580 590 600
KVDVGEINGK FSPLNNDTIA EPEFCGFRTA SNKAIPISEK MKIKTAEFMA
610 620 630 640 650
EFQSKETIQQ NDYLVHQPND KPTSVGLDTA LKRSIESSEE MRSKASKLVV
660 670 680 690 700
VDTTMREPHQ PTLDPVCRDL NESQFFGFRT ASNKAIEITE AMEKRGAMFL
710 720 730 740 750
AQSKATDQLN GWQPSDFPDV LPTSPNNEIH SINVENNKAV NTKTVSETEF
760 770 780 790 800
FGFRTASNKG IVISENTKIK VAQFMSEFQA ADASTDSNQP TVISEESRNI
810 820 830 840 850
DAKFVDEAAA EDSANKPTFC NVQSLKNTSD IEHFKHDFFV EHSAKEEHPL
860 870 880 890 900
CSQPLVRTPR RSQEIHSSLS QLAGKSPLDQ ATKKSVIARR NLLSLKRKRK
910 920 930 940 950
IILSTETSTS CALPTMERFA PKPSSTSTPL ADRDLNRSKD CTKNRQDAED
960 970
MSPICMQPKK SRRLGLSRSR Y
Length:971
Mass (Da):108,649
Last modified:November 13, 2007 - v3
Checksum:i9232DE2A667672CE
GO

Sequence cautioni

The sequence AAM51132.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti502 – 5021F → S in AAM51132 (PubMed:12537569).Curated
Sequence conflicti567 – 5671D → E in AAM51132 (PubMed:12537569).Curated
Sequence conflicti621 – 6211K → Y in AAM51132 (PubMed:12537569).Curated
Sequence conflicti650 – 6501V → D in AAM51132 (PubMed:12537569).Curated
Sequence conflicti791 – 7911T → I in AAM51132 (PubMed:12537569).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti242 – 2421E → K in strain: Zimbabwe30. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47220.3.
BT023811 mRNA. Translation: AAZ66318.1.
EU541213 Genomic DNA. Translation: ACB30270.1.
EU541214 Genomic DNA. Translation: ACB30271.1.
AY119272 mRNA. Translation: AAM51132.1. Different initiation.
RefSeqiNP_611925.2. NM_138081.4.
UniGeneiDm.13825.

Genome annotation databases

EnsemblMetazoaiFBtr0072302; FBpp0072209; FBgn0050169.
GeneIDi37916.
KEGGidme:Dmel_CG30169.
UCSCiCG30169-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF47220.3.
BT023811 mRNA. Translation: AAZ66318.1.
EU541213 Genomic DNA. Translation: ACB30270.1.
EU541214 Genomic DNA. Translation: ACB30271.1.
AY119272 mRNA. Translation: AAM51132.1. Different initiation.
RefSeqiNP_611925.2. NM_138081.4.
UniGeneiDm.13825.

3D structure databases

ProteinModelPortaliQ9W157.
SMRiQ9W157. Positions 466-496, 572-603, 674-703, 749-779.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63488. 7 interactions.
IntActiQ9W157. 8 interactions.
MINTiMINT-1015104.
STRINGi7227.FBpp0072209.

Proteomic databases

PaxDbiQ9W157.
PRIDEiQ9W157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072302; FBpp0072209; FBgn0050169.
GeneIDi37916.
KEGGidme:Dmel_CG30169.
UCSCiCG30169-RA. d. melanogaster.

Organism-specific databases

CTDi675.
FlyBaseiFBgn0050169. Brca2.

Phylogenomic databases

eggNOGiENOG410J3H8. Eukaryota.
ENOG410ZXUJ. LUCA.
InParanoidiQ9W157.
OMAiQWPGMDV.
OrthoDBiEOG7GTT2Z.
PhylomeDBiQ9W157.

Miscellaneous databases

GenomeRNAii37916.
PROiQ9W157.

Gene expression databases

BgeeiQ9W157.
GenevisibleiQ9W157. DM.

Family and domain databases

InterProiIPR015525. BRCA2.
IPR002093. BRCA2_repeat.
[Graphical view]
PANTHERiPTHR11289. PTHR11289. 1 hit.
PROSITEiPS50138. BRCA2_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Larva and Pupae.
  4. "Disruption of Drosophila melanogaster dmbrca2 (CG30169) affects rates of female meiotic crossing over."
    Barnwell C.V., Kommaraju K.R., Noor M.A.
    Dros. Info. Service 91:1-4(2008)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 234-278, VARIANT LYS-242.
    Strain: Oregon-RImported and Zimbabwe30Imported.
  5. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 276-971.
    Strain: Berkeley1 Publication.
    Tissue: Embryo1 Publication.
  6. "Functional analysis of Drosophila melanogaster BRCA2 in DNA repair."
    Brough R., Wei D., Leulier S., Lord C.J., Rong Y.S., Ashworth A.
    DNA Repair 7:10-19(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SPN-A, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  7. "Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint."
    Klovstad M., Abdu U., Schupbach T.
    PLoS Genet. 4:E31-E31(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RAD9, DISRUPTION PHENOTYPE.
  8. "Cyclin G is involved in meiotic recombination repair in Drosophila melanogaster."
    Nagel A.C., Fischer P., Szawinski J., La Rosa M.K., Preiss A.
    J. Cell Sci. 125:5555-5563(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CYCG.

Entry informationi

Entry nameiBRCA2_DROME
AccessioniPrimary (citable) accession number: Q9W157
Secondary accession number(s): B2CPK7
, B2CPK8, Q494G5, Q8MRU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: November 13, 2007
Last modified: June 8, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.