ID   PAIN_DROME              Reviewed;         913 AA.
AC   Q9W0Y6;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Transient receptor potential cation channel protein painless;
GN   Name=pain; ORFNames=CG15860;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=12705873; DOI=10.1016/s0092-8674(03)00272-1;
RA   Tracey W.D. Jr., Wilson R.I., Laurent G., Benzer S.;
RT   "painless, a Drosophila gene essential for nociception.";
RL   Cell 113:261-273(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RA   Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA   Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Receptor-activated non-selective cation channel involved in
CC       detection of pain sensation due to high temperature. Involved in heat
CC       nociception by being activated by noxious temperature of 38 degrees
CC       Celsius. {ECO:0000269|PubMed:12705873}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Present in multidendritic neurons, chordotonal
CC       neurons, a subset of cells in the central nervous system and a subset
CC       of sensory neurons in the antennal-maxillary complex. Not detected in
CC       gonads and dorsal vessels (at protein level). Expressed in peripheral
CC       neurons that extend multiple branched dendrites beneath the larval
CC       epidermis, similar to vertebrate pain receptors.
CC       {ECO:0000269|PubMed:12705873}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from stage 13 of embryonic development
CC       in a small number of cells in the central nervous system and in a
CC       subset of neurons of the peripheral nervous system. Such cells may
CC       include sensory precursors of multidendritic (md) neurons. At embryonic
CC       stage 16, prior to when dendritic process are elaborated, it appears to
CC       be expressed in multidendritic neurons in a polarized manner. At stage
CC       17 of development, when the md neurons first initiate dendritogenesis,
CC       it is localized to branched projections initiating from clusters of
CC       multidendritic neurons.
CC   -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family.
CC       {ECO:0000305}.
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DR   EMBL; AY268106; AAP03646.1; -; mRNA.
DR   EMBL; AE013599; AAF47293.1; -; Genomic_DNA.
DR   EMBL; BT011483; AAR99141.1; -; mRNA.
DR   RefSeq; NP_611979.1; NM_138135.2.
DR   AlphaFoldDB; Q9W0Y6; -.
DR   SMR; Q9W0Y6; -.
DR   BioGRID; 63553; 2.
DR   IntAct; Q9W0Y6; 1.
DR   STRING; 7227.FBpp0072323; -.
DR   SwissPalm; Q9W0Y6; -.
DR   PaxDb; 7227-FBpp0072323; -.
DR   DNASU; 37985; -.
DR   EnsemblMetazoa; FBtr0072417; FBpp0072323; FBgn0060296.
DR   GeneID; 37985; -.
DR   KEGG; dme:Dmel_CG15860; -.
DR   UCSC; CG15860-RA; d. melanogaster.
DR   AGR; FB:FBgn0060296; -.
DR   CTD; 37985; -.
DR   FlyBase; FBgn0060296; pain.
DR   VEuPathDB; VectorBase:FBgn0060296; -.
DR   eggNOG; KOG0510; Eukaryota.
DR   GeneTree; ENSGT00940000170332; -.
DR   InParanoid; Q9W0Y6; -.
DR   OMA; HTSIYEK; -.
DR   OrthoDB; 3670552at2759; -.
DR   PhylomeDB; Q9W0Y6; -.
DR   BioGRID-ORCS; 37985; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 37985; -.
DR   PRO; PR:Q9W0Y6; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0060296; Expressed in oviduct (Drosophila) and 43 other cell types or tissues.
DR   ExpressionAtlas; Q9W0Y6; baseline and differential.
DR   GO; GO:0034704; C:calcium channel complex; IDA:FlyBase.
DR   GO; GO:0016020; C:membrane; NAS:FlyBase.
DR   GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR   GO; GO:0005262; F:calcium channel activity; IDA:FlyBase.
DR   GO; GO:0005227; F:calcium-activated cation channel activity; IDA:FlyBase.
DR   GO; GO:0005216; F:monoatomic ion channel activity; TAS:FlyBase.
DR   GO; GO:0048266; P:behavioral response to pain; IMP:FlyBase.
DR   GO; GO:0070588; P:calcium ion transmembrane transport; IDA:FlyBase.
DR   GO; GO:0006816; P:calcium ion transport; ISS:FlyBase.
DR   GO; GO:0007620; P:copulation; IMP:FlyBase.
DR   GO; GO:0050974; P:detection of mechanical stimulus involved in sensory perception; TAS:FlyBase.
DR   GO; GO:0050966; P:detection of mechanical stimulus involved in sensory perception of pain; IMP:UniProtKB.
DR   GO; GO:0050965; P:detection of temperature stimulus involved in sensory perception of pain; IMP:UniProtKB.
DR   GO; GO:0050960; P:detection of temperature stimulus involved in thermoception; IDA:FlyBase.
DR   GO; GO:0007631; P:feeding behavior; IMP:FlyBase.
DR   GO; GO:0035179; P:larval turning behavior; IMP:FlyBase.
DR   GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR   GO; GO:0007638; P:mechanosensory behavior; IMP:FlyBase.
DR   GO; GO:0048060; P:negative gravitaxis; IMP:FlyBase.
DR   GO; GO:0042048; P:olfactory behavior; IMP:FlyBase.
DR   GO; GO:0045924; P:regulation of female receptivity; IMP:FlyBase.
DR   GO; GO:0008016; P:regulation of heart contraction; IMP:FlyBase.
DR   GO; GO:0009408; P:response to heat; IMP:FlyBase.
DR   GO; GO:0009612; P:response to mechanical stimulus; NAS:FlyBase.
DR   GO; GO:0019233; P:sensory perception of pain; IMP:FlyBase.
DR   Gene3D; 1.10.287.70; -; 1.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 2.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR005821; Ion_trans_dom.
DR   PANTHER; PTHR47143; TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS; 1.
DR   PANTHER; PTHR47143:SF3; TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS; 1.
DR   Pfam; PF00520; Ion_trans; 1.
DR   SMART; SM00248; ANK; 4.
DR   SUPFAM; SSF48403; Ankyrin repeat; 2.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   Genevisible; Q9W0Y6; DM.
PE   1: Evidence at protein level;
KW   ANK repeat; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Repeat; Sensory transduction; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..913
FT                   /note="Transient receptor potential cation channel protein
FT                   painless"
FT                   /id="PRO_0000215374"
FT   TOPO_DOM        1..490
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        491..511
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        512..523
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        524..544
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        545..555
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        556..576
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        577..586
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        587..607
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        608..628
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        629..649
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        650..708
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        709..729
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        730..913
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REPEAT          154..189
FT                   /note="ANK 1"
FT   REPEAT          260..289
FT                   /note="ANK 2"
FT   REPEAT          368..397
FT                   /note="ANK 3"
FT   REGION          656..675
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   913 AA;  103507 MW;  D89CC6082E1A03C5 CRC64;
     MDFNNCGFID PQAQLAGALA KQDIRQFVAA LDSGALADLQ DDRHTSIYEK ALSTPGCRDF
     IEACIDHGSQ VNYINKKLDK AAISYAADSR DPGNLAALLK YRPGNKVQVD RKYGQLTPLN
     SLAKNLTDEN APDVYSCMQL LLDYGASPNI VDQGEFTPLH HVLRKSKVKA GKKELIQLFL
     DHPELDIDSY RNGEVRRLLQ AQFPELKLPE ERHTGPEIDI QTLQRTLRDG DETLFEQQFA
     EYLQNLKGGA DNQLNAHQEE YFGLLQESIK RGRQRAFDVI LSTGMDINSR PGRANEANLV
     ETAVIYGNWQ ALERLLKEPN LRLTPDSKLL NAVIGRLDEP PYDGSSHQRC FELLINSDRV
     DINEADSGRL VPLFFAVKYR NTSAMQKLLK NGAYIGSKSA FGTLPIKDMP PEVLEEHFDS
     CITTNGERPG DQNFEIIIDY KNLMRQERDS GLNQLQDEMA PIAFIAESKE MRHLLQHPLI
     SSFLFLKWHR LSVIFYLNFL IYSLFTASII TYTLLKFHES DQRALTAFFG LLSWLGISYL
     ILRECIQWIM SPVRYFWSIT NIMEVALITL SIFTCMESSF DKETQRVLAV FTILLVSMEF
     CLLVGSLPVL SISTHMLMLR EVSNSFLKSF TLYSIFVLTF SLCFYILFGK SVEEDQSKSA
     TPCPPLGKKE GKDEEQGFNT FTKPIEAVIK TIVMLTGEFD AGSIQFTSIY TYLIFLLFVI
     FMTIVLFNLL NGLAVSDTQV IKAQAELNGA ICRTNVLSRY EQVLTGHGRA GFLLGNHLFR
     SICQRLMNIY PNYLSLRQIS VLPNDGNKVL IPMSDPFEMR TLKKASFQQL PLSAAVPQKK
     LLDPPLRLLP CCCSLLTGKC SQMSGRVVKR ALEVIDQKNA AEQRRKQEQI NDSRLKLIEY
     KLEQLIQLVQ DRK
//