Q9W0Y6 (PAIN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transient receptor potential cation channel protein painless

Gene names

Name:	pain
ORF Names:	CG15860

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. Ref.1
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. Ref.1
Developmental stage	Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons.
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. [View classification] Contains 3 ANK repeats.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Molecular function	Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	behavioral response to pain Inferred from mutant phenotype PubMed 19995327. Source: FlyBase copulation Inferred from mutant phenotype PubMed 19531155. Source: FlyBase detection of mechanical stimulus involved in sensory perception of pain Inferred from mutant phenotype Ref.1. Source: UniProtKB detection of temperature stimulus involved in sensory perception of pain Inferred from mutant phenotype Ref.1. Source: UniProtKB feeding behavior Inferred from mutant phenotype PubMed 16647259. Source: FlyBase male courtship behavior Inferred from mutant phenotype PubMed 22073144. Source: FlyBase mechanosensory behavior Inferred from mutant phenotype PubMed 22343891. Source: FlyBase negative gravitaxis Inferred from mutant phenotype PubMed 19666538. Source: FlyBase olfactory behavior Inferred from mutant phenotype PubMed 22073144. Source: FlyBase regulation of female receptivity Inferred from mutant phenotype PubMed 19531155. Source: FlyBase regulation of heart contraction Inferred from mutant phenotype PubMed 20824071. Source: FlyBase response to heat Inferred from mutant phenotype PubMed 19995327. Source: FlyBase
Cellular_component	integral to membrane Non-traceable author statement PubMed 12814565. Source: FlyBase
Molecular_function	calcium channel activity Inferred from sequence or structural similarity PubMed 10798390. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 913	913	Transient receptor potential cation channel protein painless		PRO_0000215374
Regions
	Topological domain	1 – 490	490	Cytoplasmic Potential
	Transmembrane	491 – 511	21	Helical; Name=1; Potential
	Topological domain	512 – 523	12	Extracellular Potential
	Transmembrane	524 – 544	21	Helical; Name=2; Potential
	Topological domain	545 – 555	11	Cytoplasmic Potential
	Transmembrane	556 – 576	21	Helical; Name=3; Potential
	Topological domain	577 – 586	10	Extracellular Potential
	Transmembrane	587 – 607	21	Helical; Name=4; Potential
	Topological domain	608 – 628	21	Cytoplasmic Potential
	Transmembrane	629 – 649	21	Helical; Name=5; Potential
	Topological domain	650 – 708	59	Extracellular Potential
	Transmembrane	709 – 729	21	Helical; Name=6; Potential
	Topological domain	730 – 913	184	Cytoplasmic Potential
	Repeat	154 – 189	36	ANK 1
	Repeat	260 – 289	30	ANK 2
	Repeat	368 – 397	30	ANK 3

Sequences

	Sequence		Length	Mass (Da)
	Q9W0Y6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D89CC6082E1A03C5	FASTA	913	103,507
	10 20 30 40 50 60 MDFNNCGFID PQAQLAGALA KQDIRQFVAA LDSGALADLQ DDRHTSIYEK ALSTPGCRDF 70 80 90 100 110 120 IEACIDHGSQ VNYINKKLDK AAISYAADSR DPGNLAALLK YRPGNKVQVD RKYGQLTPLN 130 140 150 160 170 180 SLAKNLTDEN APDVYSCMQL LLDYGASPNI VDQGEFTPLH HVLRKSKVKA GKKELIQLFL 190 200 210 220 230 240 DHPELDIDSY RNGEVRRLLQ AQFPELKLPE ERHTGPEIDI QTLQRTLRDG DETLFEQQFA 250 260 270 280 290 300 EYLQNLKGGA DNQLNAHQEE YFGLLQESIK RGRQRAFDVI LSTGMDINSR PGRANEANLV 310 320 330 340 350 360 ETAVIYGNWQ ALERLLKEPN LRLTPDSKLL NAVIGRLDEP PYDGSSHQRC FELLINSDRV 370 380 390 400 410 420 DINEADSGRL VPLFFAVKYR NTSAMQKLLK NGAYIGSKSA FGTLPIKDMP PEVLEEHFDS 430 440 450 460 470 480 CITTNGERPG DQNFEIIIDY KNLMRQERDS GLNQLQDEMA PIAFIAESKE MRHLLQHPLI 490 500 510 520 530 540 SSFLFLKWHR LSVIFYLNFL IYSLFTASII TYTLLKFHES DQRALTAFFG LLSWLGISYL 550 560 570 580 590 600 ILRECIQWIM SPVRYFWSIT NIMEVALITL SIFTCMESSF DKETQRVLAV FTILLVSMEF 610 620 630 640 650 660 CLLVGSLPVL SISTHMLMLR EVSNSFLKSF TLYSIFVLTF SLCFYILFGK SVEEDQSKSA 670 680 690 700 710 720 TPCPPLGKKE GKDEEQGFNT FTKPIEAVIK TIVMLTGEFD AGSIQFTSIY TYLIFLLFVI 730 740 750 760 770 780 FMTIVLFNLL NGLAVSDTQV IKAQAELNGA ICRTNVLSRY EQVLTGHGRA GFLLGNHLFR 790 800 810 820 830 840 SICQRLMNIY PNYLSLRQIS VLPNDGNKVL IPMSDPFEMR TLKKASFQQL PLSAAVPQKK 850 860 870 880 890 900 LLDPPLRLLP CCCSLLTGKC SQMSGRVVKR ALEVIDQKNA AEQRRKQEQI NDSRLKLIEY 910 KLEQLIQLVQ DRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"painless, a Drosophila gene essential for nociception." Tracey W.D. Jr., Wilson R.I., Laurent G., Benzer S. Cell 113:261-273(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY268106 mRNA. Translation: AAP03646.1. AE013599 Genomic DNA. Translation: AAF47293.1. BT011483 mRNA. Translation: AAR99141.1.
RefSeq	NP_611979.1. NM_138135.2.
UniGene	Dm.14984.
3D structure databases
ProteinModelPortal	Q9W0Y6.
SMR	Q9W0Y6. Positions 115-204, 360-409.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-894981.
STRING	7227.FBpp0072323.
Proteomic databases
PaxDb	Q9W0Y6.
PRIDE	Q9W0Y6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072417; FBpp0072323; FBgn0060296.
GeneID	37985.
KEGG	dme:Dmel_CG15860.
UCSC	CG15860-RA. d. melanogaster.
Organism-specific databases
CTD	37985.
FlyBase	FBgn0060296. pain.
Phylogenomic databases
eggNOG	NOG267148.
InParanoid	Q9W0Y6.
OMA	NGAICRT.
OrthoDB	EOG4J9KF0.
PhylomeDB	Q9W0Y6.
Gene expression databases
Bgee	Q9W0Y6.
GermOnline	CG15860. Drosophila melanogaster.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Pfam	PF12796. Ank_2. 1 hit. [Graphical view]
SMART	SM00248. ANK. 4 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 2 hits.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	37985.
NextBio	806382.

Entry information

Entry name

PAIN_DROME

Accession

Primary (citable) accession number: Q9W0Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project