Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9W0Y6 (PAIN_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel protein painless
Gene names
Name:pain
ORF Names:CG15860
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length913 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. Ref.1

Developmental stage

Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. [View classification]

Contains 3 ANK repeats.

Ontologies

Keywords
   Biological processIon transport
Sensory transduction
Transport
   Cellular componentMembrane
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
   Molecular functionIon channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbehavioral response to pain

Inferred from mutant phenotype PubMed 19995327. Source: FlyBase

calcium ion transmembrane transport

Inferred from direct assay PubMed 18829951. Source: FlyBase

calcium ion transport

Inferred from sequence or structural similarity PubMed 10798390. Source: FlyBase

copulation

Inferred from mutant phenotype PubMed 19531155. Source: FlyBase

detection of mechanical stimulus involved in sensory perception

Traceable author statement PubMed 14624845. Source: FlyBase

detection of mechanical stimulus involved in sensory perception of pain

Inferred from mutant phenotype Ref.1. Source: UniProtKB

detection of temperature stimulus involved in sensory perception of pain

Inferred from mutant phenotype Ref.1. Source: UniProtKB

detection of temperature stimulus involved in thermoception

Inferred from direct assay PubMed 18829951. Source: FlyBase

feeding behavior

Inferred from mutant phenotype PubMed 16647259. Source: FlyBase

male courtship behavior

Inferred from mutant phenotype PubMed 22073144. Source: FlyBase

mechanosensory behavior

Inferred from mutant phenotype PubMed 22343891. Source: FlyBase

negative gravitaxis

Inferred from mutant phenotype PubMed 19666538. Source: FlyBase

olfactory behavior

Inferred from mutant phenotype PubMed 22073144. Source: FlyBase

regulation of female receptivity

Inferred from mutant phenotype PubMed 19531155. Source: FlyBase

regulation of heart contraction

Inferred from mutant phenotype PubMed 20824071. Source: FlyBase

response to heat

Inferred from mutant phenotype PubMed 19995327. Source: FlyBase

response to mechanical stimulus

Non-traceable author statement PubMed 12814565. Source: FlyBase

sensory perception of pain

Inferred from mutant phenotype Ref.1PubMed 21074052. Source: FlyBase

   Cellular_componentcalcium channel complex

Inferred from direct assay PubMed 18829951. Source: FlyBase

integral component of membrane

Non-traceable author statement PubMed 12814565. Source: FlyBase

   Molecular_functioncalcium activated cation channel activity

Inferred from direct assay PubMed 18829951. Source: FlyBase

calcium channel activity

Inferred from direct assay PubMed 18829951. Source: FlyBase

ion channel activity

Traceable author statement PubMed 14624845. Source: FlyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 913913Transient receptor potential cation channel protein painless
PRO_0000215374

Regions

Topological domain1 – 490490Cytoplasmic Potential
Transmembrane491 – 51121Helical; Name=1; Potential
Topological domain512 – 52312Extracellular Potential
Transmembrane524 – 54421Helical; Name=2; Potential
Topological domain545 – 55511Cytoplasmic Potential
Transmembrane556 – 57621Helical; Name=3; Potential
Topological domain577 – 58610Extracellular Potential
Transmembrane587 – 60721Helical; Name=4; Potential
Topological domain608 – 62821Cytoplasmic Potential
Transmembrane629 – 64921Helical; Name=5; Potential
Topological domain650 – 70859Extracellular Potential
Transmembrane709 – 72921Helical; Name=6; Potential
Topological domain730 – 913184Cytoplasmic Potential
Repeat154 – 18936ANK 1
Repeat260 – 28930ANK 2
Repeat368 – 39730ANK 3

Sequences

Sequence LengthMass (Da)Tools
Q9W0Y6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: D89CC6082E1A03C5

FASTA913103,507
        10         20         30         40         50         60 
MDFNNCGFID PQAQLAGALA KQDIRQFVAA LDSGALADLQ DDRHTSIYEK ALSTPGCRDF 

        70         80         90        100        110        120 
IEACIDHGSQ VNYINKKLDK AAISYAADSR DPGNLAALLK YRPGNKVQVD RKYGQLTPLN 

       130        140        150        160        170        180 
SLAKNLTDEN APDVYSCMQL LLDYGASPNI VDQGEFTPLH HVLRKSKVKA GKKELIQLFL 

       190        200        210        220        230        240 
DHPELDIDSY RNGEVRRLLQ AQFPELKLPE ERHTGPEIDI QTLQRTLRDG DETLFEQQFA 

       250        260        270        280        290        300 
EYLQNLKGGA DNQLNAHQEE YFGLLQESIK RGRQRAFDVI LSTGMDINSR PGRANEANLV 

       310        320        330        340        350        360 
ETAVIYGNWQ ALERLLKEPN LRLTPDSKLL NAVIGRLDEP PYDGSSHQRC FELLINSDRV 

       370        380        390        400        410        420 
DINEADSGRL VPLFFAVKYR NTSAMQKLLK NGAYIGSKSA FGTLPIKDMP PEVLEEHFDS 

       430        440        450        460        470        480 
CITTNGERPG DQNFEIIIDY KNLMRQERDS GLNQLQDEMA PIAFIAESKE MRHLLQHPLI 

       490        500        510        520        530        540 
SSFLFLKWHR LSVIFYLNFL IYSLFTASII TYTLLKFHES DQRALTAFFG LLSWLGISYL 

       550        560        570        580        590        600 
ILRECIQWIM SPVRYFWSIT NIMEVALITL SIFTCMESSF DKETQRVLAV FTILLVSMEF 

       610        620        630        640        650        660 
CLLVGSLPVL SISTHMLMLR EVSNSFLKSF TLYSIFVLTF SLCFYILFGK SVEEDQSKSA 

       670        680        690        700        710        720 
TPCPPLGKKE GKDEEQGFNT FTKPIEAVIK TIVMLTGEFD AGSIQFTSIY TYLIFLLFVI 

       730        740        750        760        770        780 
FMTIVLFNLL NGLAVSDTQV IKAQAELNGA ICRTNVLSRY EQVLTGHGRA GFLLGNHLFR 

       790        800        810        820        830        840 
SICQRLMNIY PNYLSLRQIS VLPNDGNKVL IPMSDPFEMR TLKKASFQQL PLSAAVPQKK 

       850        860        870        880        890        900 
LLDPPLRLLP CCCSLLTGKC SQMSGRVVKR ALEVIDQKNA AEQRRKQEQI NDSRLKLIEY 

       910 
KLEQLIQLVQ DRK 

« Hide

References

« Hide 'large scale' references
[1]"painless, a Drosophila gene essential for nociception."
Tracey W.D. Jr., Wilson R.I., Laurent G., Benzer S.
Cell 113:261-273(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY268106 mRNA. Translation: AAP03646.1.
AE013599 Genomic DNA. Translation: AAF47293.1.
BT011483 mRNA. Translation: AAR99141.1.
RefSeqNP_611979.1. NM_138135.2.
UniGeneDm.14984.

3D structure databases

ProteinModelPortalQ9W0Y6.
SMRQ9W0Y6. Positions 10-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid63553. 1 interaction.
MINTMINT-894981.
STRING7227.FBpp0072323.

Proteomic databases

PaxDbQ9W0Y6.
PRIDEQ9W0Y6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0072417; FBpp0072323; FBgn0060296.
GeneID37985.
KEGGdme:Dmel_CG15860.
UCSCCG15860-RA. d. melanogaster.

Organism-specific databases

CTD37985.
FlyBaseFBgn0060296. pain.

Phylogenomic databases

eggNOGNOG267148.
GeneTreeENSGT00740000115514.
InParanoidQ9W0Y6.
OMANGAICRT.
OrthoDBEOG74N5G3.
PhylomeDBQ9W0Y6.

Gene expression databases

BgeeQ9W0Y6.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
[Graphical view]
PfamPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 2 hits.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi37985.
NextBio806382.

Entry information

Entry namePAIN_DROME
AccessionPrimary (citable) accession number: Q9W0Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase