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Protein

Transient receptor potential cation channel protein painless

Gene

pain

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius.1 Publication

GO - Molecular functioni

  • calcium activated cation channel activity Source: FlyBase
  • calcium channel activity Source: FlyBase
  • ion channel activity Source: FlyBase

GO - Biological processi

  • behavioral response to pain Source: FlyBase
  • calcium ion transmembrane transport Source: FlyBase
  • calcium ion transport Source: FlyBase
  • copulation Source: FlyBase
  • detection of mechanical stimulus involved in sensory perception Source: FlyBase
  • detection of mechanical stimulus involved in sensory perception of pain Source: UniProtKB
  • detection of temperature stimulus involved in sensory perception of pain Source: UniProtKB
  • detection of temperature stimulus involved in thermoception Source: FlyBase
  • feeding behavior Source: FlyBase
  • male courtship behavior Source: FlyBase
  • mechanosensory behavior Source: FlyBase
  • negative gravitaxis Source: FlyBase
  • olfactory behavior Source: FlyBase
  • regulation of female receptivity Source: FlyBase
  • regulation of heart contraction Source: FlyBase
  • response to heat Source: FlyBase
  • response to mechanical stimulus Source: FlyBase
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel protein painless
Gene namesi
Name:pain
ORF Names:CG15860
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0060296. pain.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 490490CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei491 – 51121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini512 – 52312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei524 – 54421Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini545 – 55511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei556 – 57621Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini577 – 58610ExtracellularSequence Analysis
Transmembranei587 – 60721Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini608 – 62821CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei629 – 64921Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini650 – 70859ExtracellularSequence AnalysisAdd
BLAST
Transmembranei709 – 72921Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini730 – 913184CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • calcium channel complex Source: FlyBase
  • integral component of membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 913913Transient receptor potential cation channel protein painlessPRO_0000215374Add
BLAST

Proteomic databases

PaxDbiQ9W0Y6.
PRIDEiQ9W0Y6.

Expressioni

Tissue specificityi

Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors.1 Publication

Developmental stagei

Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons.

Gene expression databases

BgeeiQ9W0Y6.
GenevisibleiQ9W0Y6. DM.

Interactioni

Protein-protein interaction databases

BioGridi63553. 1 interaction.
IntActiQ9W0Y6. 1 interaction.
MINTiMINT-894981.
STRINGi7227.FBpp0072323.

Structurei

3D structure databases

ProteinModelPortaliQ9W0Y6.
SMRiQ9W0Y6. Positions 349-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati154 – 18936ANK 1Add
BLAST
Repeati260 – 28930ANK 2Add
BLAST
Repeati368 – 39730ANK 3Add
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG267148.
GeneTreeiENSGT00780000121959.
InParanoidiQ9W0Y6.
OMAiFDSCITT.
OrthoDBiEOG74N5G3.
PhylomeDBiQ9W0Y6.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR030288. TRPA1/painless.
[Graphical view]
PANTHERiPTHR24190:SF5. PTHR24190:SF5. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9W0Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFNNCGFID PQAQLAGALA KQDIRQFVAA LDSGALADLQ DDRHTSIYEK
60 70 80 90 100
ALSTPGCRDF IEACIDHGSQ VNYINKKLDK AAISYAADSR DPGNLAALLK
110 120 130 140 150
YRPGNKVQVD RKYGQLTPLN SLAKNLTDEN APDVYSCMQL LLDYGASPNI
160 170 180 190 200
VDQGEFTPLH HVLRKSKVKA GKKELIQLFL DHPELDIDSY RNGEVRRLLQ
210 220 230 240 250
AQFPELKLPE ERHTGPEIDI QTLQRTLRDG DETLFEQQFA EYLQNLKGGA
260 270 280 290 300
DNQLNAHQEE YFGLLQESIK RGRQRAFDVI LSTGMDINSR PGRANEANLV
310 320 330 340 350
ETAVIYGNWQ ALERLLKEPN LRLTPDSKLL NAVIGRLDEP PYDGSSHQRC
360 370 380 390 400
FELLINSDRV DINEADSGRL VPLFFAVKYR NTSAMQKLLK NGAYIGSKSA
410 420 430 440 450
FGTLPIKDMP PEVLEEHFDS CITTNGERPG DQNFEIIIDY KNLMRQERDS
460 470 480 490 500
GLNQLQDEMA PIAFIAESKE MRHLLQHPLI SSFLFLKWHR LSVIFYLNFL
510 520 530 540 550
IYSLFTASII TYTLLKFHES DQRALTAFFG LLSWLGISYL ILRECIQWIM
560 570 580 590 600
SPVRYFWSIT NIMEVALITL SIFTCMESSF DKETQRVLAV FTILLVSMEF
610 620 630 640 650
CLLVGSLPVL SISTHMLMLR EVSNSFLKSF TLYSIFVLTF SLCFYILFGK
660 670 680 690 700
SVEEDQSKSA TPCPPLGKKE GKDEEQGFNT FTKPIEAVIK TIVMLTGEFD
710 720 730 740 750
AGSIQFTSIY TYLIFLLFVI FMTIVLFNLL NGLAVSDTQV IKAQAELNGA
760 770 780 790 800
ICRTNVLSRY EQVLTGHGRA GFLLGNHLFR SICQRLMNIY PNYLSLRQIS
810 820 830 840 850
VLPNDGNKVL IPMSDPFEMR TLKKASFQQL PLSAAVPQKK LLDPPLRLLP
860 870 880 890 900
CCCSLLTGKC SQMSGRVVKR ALEVIDQKNA AEQRRKQEQI NDSRLKLIEY
910
KLEQLIQLVQ DRK
Length:913
Mass (Da):103,507
Last modified:May 1, 2000 - v1
Checksum:iD89CC6082E1A03C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY268106 mRNA. Translation: AAP03646.1.
AE013599 Genomic DNA. Translation: AAF47293.1.
BT011483 mRNA. Translation: AAR99141.1.
RefSeqiNP_611979.1. NM_138135.2.
UniGeneiDm.14984.

Genome annotation databases

EnsemblMetazoaiFBtr0072417; FBpp0072323; FBgn0060296.
GeneIDi37985.
KEGGidme:Dmel_CG15860.
UCSCiCG15860-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY268106 mRNA. Translation: AAP03646.1.
AE013599 Genomic DNA. Translation: AAF47293.1.
BT011483 mRNA. Translation: AAR99141.1.
RefSeqiNP_611979.1. NM_138135.2.
UniGeneiDm.14984.

3D structure databases

ProteinModelPortaliQ9W0Y6.
SMRiQ9W0Y6. Positions 349-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63553. 1 interaction.
IntActiQ9W0Y6. 1 interaction.
MINTiMINT-894981.
STRINGi7227.FBpp0072323.

Proteomic databases

PaxDbiQ9W0Y6.
PRIDEiQ9W0Y6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072417; FBpp0072323; FBgn0060296.
GeneIDi37985.
KEGGidme:Dmel_CG15860.
UCSCiCG15860-RA. d. melanogaster.

Organism-specific databases

CTDi37985.
FlyBaseiFBgn0060296. pain.

Phylogenomic databases

eggNOGiNOG267148.
GeneTreeiENSGT00780000121959.
InParanoidiQ9W0Y6.
OMAiFDSCITT.
OrthoDBiEOG74N5G3.
PhylomeDBiQ9W0Y6.

Miscellaneous databases

GenomeRNAii37985.
NextBioi806382.
PROiQ9W0Y6.

Gene expression databases

BgeeiQ9W0Y6.
GenevisibleiQ9W0Y6. DM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR030288. TRPA1/painless.
[Graphical view]
PANTHERiPTHR24190:SF5. PTHR24190:SF5. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "painless, a Drosophila gene essential for nociception."
    Tracey W.D. Jr., Wilson R.I., Laurent G., Benzer S.
    Cell 113:261-273(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiPAIN_DROME
AccessioniPrimary (citable) accession number: Q9W0Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: May 1, 2000
Last modified: June 24, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.