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Protein

3-phosphoinositide-dependent protein kinase 1

Gene

Pdk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase required for embryonic development. Inhibits apoptosis. Acts in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and activating Akt1 and S6k. May be involved in axonal pathfinding and synaptogenesis, and in spermatogenesis.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei275ATPBy similarity1
Binding sitei331ATPBy similarity1
Active sitei370Proton acceptorPROSITE-ProRule annotation1
Binding sitei374ATP; via carbonyl oxygenBy similarity1
Binding sitei388ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 258ATPBy similarity3
Nucleotide bindingi325 – 327ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: FlyBase
  • cellular response to DNA damage stimulus Source: FlyBase
  • female gamete generation Source: UniProtKB
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • insulin receptor signaling pathway Source: FlyBase
  • intracellular signal transduction Source: GO_Central
  • negative regulation of apoptotic process Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of cell growth Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • positive regulation of multicellular organism growth Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell growth Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of organ growth Source: FlyBase
  • response to ethanol Source: FlyBase
  • sex differentiation Source: UniProtKB
  • spermatogenesis Source: UniProtKB
  • synaptic growth at neuromuscular junction Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Differentiation, Growth regulation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-114604. GPVI-mediated activation cascade.
R-DME-1257604. PIP3 activates AKT signaling.
R-DME-165158. Activation of AKT2.
R-DME-202424. Downstream TCR signaling.
R-DME-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-389357. CD28 dependent PI3K/Akt signaling.
R-DME-392451. G beta:gamma signalling through PI3Kgamma.
R-DME-444257. RSK activation.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-5218921. VEGFR2 mediated cell proliferation.
R-DME-5625740. RHO GTPases activate PKNs.
SignaLinkiQ9W0V1.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoinositide-dependent protein kinase 1 (EC:2.7.11.1)
Short name:
dPDK-1
Alternative name(s):
Serine/threonine-protein kinase 61C
dSTPK61
Gene namesi
Name:Pdk1
Synonyms:Pk61C
ORF Names:CG1210
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0020386. Pdk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: FlyBase
  • neuromuscular junction Source: FlyBase
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death during the second instar stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865041 – 8363-phosphoinositide-dependent protein kinase 1Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei641Phosphothreonine1 Publication1
Modified residuei645Phosphoserine1 Publication1
Modified residuei804Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9W0V1.
PRIDEiQ9W0V1.

PTM databases

iPTMnetiQ9W0V1.

Expressioni

Developmental stagei

Highly expressed in Malpighian tubule primordia from stage late 11 to 13. Expressed in hindgut at stage 13. After stage 14, ubiquitously expressed at low levels.1 Publication

Gene expression databases

BgeeiFBgn0020386.
ExpressionAtlasiQ9W0V1. baseline.
GenevisibleiQ9W0V1. DM.

Interactioni

Protein-protein interaction databases

BioGridi63582. 12 interactors.
DIPiDIP-20399N.
IntActiQ9W0V1. 3 interactors.
MINTiMINT-782747.
STRINGi7227.FBpp0305420.

Structurei

3D structure databases

ProteinModelPortaliQ9W0V1.
SMRiQ9W0V1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini246 – 571Protein kinasePROSITE-ProRule annotationAdd BLAST326

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 322PIF-pocketBy similarityAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 235Gln-richAdd BLAST54
Compositional biasi768 – 804Ser-richAdd BLAST37

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0592. Eukaryota.
ENOG410XRT8. LUCA.
GeneTreeiENSGT00550000074819.
InParanoidiQ9W0V1.
KOiK06276.
OMAiVYLAVDI.
OrthoDBiEOG091G06CX.
PhylomeDBiQ9W0V1.

Family and domain databases

CDDicd01262. PH_PDK1. 1 hit.
Gene3Di2.30.29.30. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR033931. PDK1-typ_PH.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF14593. PH_3. 1 hit.
PF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q9W0V1-4) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKCKSWSNKI NNYVVRKIKS IKINGTQQQL QLPGSGASGI AAAAVITVAS
60 70 80 90 100
DCGENCSSNG TEHQQHFNIA TTTATSATEA TMPAMAKEKA SATVSLGESN
110 120 130 140 150
FRDINLKDLA VVVEAASRLH HQQNVCGCGA VSSTENNNNS RYGSSKYLTN
160 170 180 190 200
GHTSPLAAAV ASNSSSVATT PHCRMLHNCS LQQYQNDIRQ QTEILDMLRQ
210 220 230 240 250
QHQQGYQSQQ QQQQPQQQQE QQQQQEQSQQ QQQLQNPAPR RSPNDFIFGR
260 270 280 290 300
YIGEGSYSIV YLAVDIHSRR EYAIKVCEKR LILRERKQDY IKREREVMHQ
310 320 330 340 350
MTNVPGFVNL SCTFQDQRSL YFVMTYARKG DMLPYINRVG SFDVACTRHY
360 370 380 390 400
AAELLLACEH MHRRNVVHRD LKPENILLDE DMHTLIADFG SAKVMTAHER
410 420 430 440 450
ALATEHCSEQ RRSNSDEDDE DSDRLENEDE DFYDRDSEEL DDGDDEQQQE
460 470 480 490 500
EMDSPRHRQR RYNRHRKASF VGTAQYVSPE VLQNGPITPA ADLWALGCIV
510 520 530 540 550
YQMIAGLPPF RGSNDYVIFK EILDCAVDFP QGFDKDAEDL VRKLLRVDPR
560 570 580 590 600
DRLGAQDEFG YYESIRAHPF FAGIDWQTLR QQTPPPIYPY LPGVSQDEDF
610 620 630 640 650
RSSYTVPGDL EPGLDERQIS RLLSAELGVG SSVAMPVKRS TAKNSFDLND
660 670 680 690 700
AEKLQRLEQQ KTDKWHVFAD GEVILKKGFV NKRKGLFARK RMLLLTTGPR
710 720 730 740 750
LIYIDPVQMI KKGEIPWSPD LRAEYKNFKI FFVHTPNRTY YLDDPEGYAI
760 770 780 790 800
HWSEAIENMR KLAYGDPSST SAVSCSSGSS NSLAVISNSS AASSSNSPTV
810 820 830
KRSSPVNAPQ ASTASDNRTL GSTRTGTSPS KKTASK
Note: No experimental confirmation available.
Length:836
Mass (Da):94,079
Last modified:January 24, 2006 - v4
Checksum:iCA274D2265D17AE2
GO
Isoform 1 (identifier: Q9W0V1-1) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MKCKSWSNKINNYVVRKIKSIK → MNIIQ

Show »
Length:819
Mass (Da):92,028
Checksum:iEA36359C8364A3A7
GO
Isoform 2 (identifier: Q9W0V1-2) [UniParc]FASTAAdd to basket
Also known as: A, F

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Show »
Length:755
Mass (Da):85,613
Checksum:i6375A99A25089FDD
GO
Isoform 3 (identifier: Q9W0V1-3) [UniParc]FASTAAdd to basket
Also known as: B, E

The sequence of this isoform differs from the canonical sequence as follows:
     1-297: Missing.

Note: No experimental confirmation available.
Show »
Length:539
Mass (Da):60,954
Checksum:iB9B864D2527D7260
GO

Sequence cautioni

The sequence AAN71019 differs from that shown. Reason: Frameshift at positions 470 and 733.Curated
The sequence CAA69216 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200 – 201QQ → HE in CAA69216 (PubMed:10336630).Curated2
Sequence conflicti443G → R in CAA69216 (PubMed:10336630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0048971 – 297Missing in isoform 3. 1 PublicationAdd BLAST297
Alternative sequenceiVSP_0048961 – 81Missing in isoform 2. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_0170831 – 22MKCKS…IKSIK → MNIIQ in isoform 1. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07908 mRNA. Translation: CAA69216.1. Different initiation.
AE014296 Genomic DNA. Translation: AAF47327.3.
AE014296 Genomic DNA. Translation: AAF47329.2.
AE014296 Genomic DNA. Translation: AAF47330.3.
AE014296 Genomic DNA. Translation: AAF47332.1.
BT004489 mRNA. Translation: AAO42653.1.
BT001263 mRNA. Translation: AAN71019.1. Frameshift.
BT011471 mRNA. Translation: AAR99129.1.
RefSeqiNP_001261183.1. NM_001274254.1. [Q9W0V1-4]
NP_525121.2. NM_080382.4. [Q9W0V1-2]
NP_728470.1. NM_167789.2. [Q9W0V1-1]
NP_728471.2. NM_167790.3. [Q9W0V1-4]
NP_728472.1. NM_167791.3. [Q9W0V1-2]
NP_728473.1. NM_167792.3. [Q9W0V1-2]
NP_728474.1. NM_167793.3. [Q9W0V1-3]
NP_728475.1. NM_167794.3. [Q9W0V1-3]
NP_728476.1. NM_167795.3. [Q9W0V1-3]
UniGeneiDm.7033.

Genome annotation databases

EnsemblMetazoaiFBtr0072464; FBpp0072366; FBgn0020386. [Q9W0V1-4]
FBtr0333218; FBpp0305420; FBgn0020386. [Q9W0V1-4]
GeneIDi38017.
KEGGidme:Dmel_CG1210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07908 mRNA. Translation: CAA69216.1. Different initiation.
AE014296 Genomic DNA. Translation: AAF47327.3.
AE014296 Genomic DNA. Translation: AAF47329.2.
AE014296 Genomic DNA. Translation: AAF47330.3.
AE014296 Genomic DNA. Translation: AAF47332.1.
BT004489 mRNA. Translation: AAO42653.1.
BT001263 mRNA. Translation: AAN71019.1. Frameshift.
BT011471 mRNA. Translation: AAR99129.1.
RefSeqiNP_001261183.1. NM_001274254.1. [Q9W0V1-4]
NP_525121.2. NM_080382.4. [Q9W0V1-2]
NP_728470.1. NM_167789.2. [Q9W0V1-1]
NP_728471.2. NM_167790.3. [Q9W0V1-4]
NP_728472.1. NM_167791.3. [Q9W0V1-2]
NP_728473.1. NM_167792.3. [Q9W0V1-2]
NP_728474.1. NM_167793.3. [Q9W0V1-3]
NP_728475.1. NM_167794.3. [Q9W0V1-3]
NP_728476.1. NM_167795.3. [Q9W0V1-3]
UniGeneiDm.7033.

3D structure databases

ProteinModelPortaliQ9W0V1.
SMRiQ9W0V1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63582. 12 interactors.
DIPiDIP-20399N.
IntActiQ9W0V1. 3 interactors.
MINTiMINT-782747.
STRINGi7227.FBpp0305420.

PTM databases

iPTMnetiQ9W0V1.

Proteomic databases

PaxDbiQ9W0V1.
PRIDEiQ9W0V1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072464; FBpp0072366; FBgn0020386. [Q9W0V1-4]
FBtr0333218; FBpp0305420; FBgn0020386. [Q9W0V1-4]
GeneIDi38017.
KEGGidme:Dmel_CG1210.

Organism-specific databases

CTDi5163.
FlyBaseiFBgn0020386. Pdk1.

Phylogenomic databases

eggNOGiKOG0592. Eukaryota.
ENOG410XRT8. LUCA.
GeneTreeiENSGT00550000074819.
InParanoidiQ9W0V1.
KOiK06276.
OMAiVYLAVDI.
OrthoDBiEOG091G06CX.
PhylomeDBiQ9W0V1.

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-114604. GPVI-mediated activation cascade.
R-DME-1257604. PIP3 activates AKT signaling.
R-DME-165158. Activation of AKT2.
R-DME-202424. Downstream TCR signaling.
R-DME-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-389357. CD28 dependent PI3K/Akt signaling.
R-DME-392451. G beta:gamma signalling through PI3Kgamma.
R-DME-444257. RSK activation.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-5218921. VEGFR2 mediated cell proliferation.
R-DME-5625740. RHO GTPases activate PKNs.
SignaLinkiQ9W0V1.

Miscellaneous databases

ChiTaRSiPdk1. fly.
GenomeRNAii38017.
PROiQ9W0V1.

Gene expression databases

BgeeiFBgn0020386.
ExpressionAtlasiQ9W0V1. baseline.
GenevisibleiQ9W0V1. DM.

Family and domain databases

CDDicd01262. PH_PDK1. 1 hit.
Gene3Di2.30.29.30. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR033931. PDK1-typ_PH.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF14593. PH_3. 1 hit.
PF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDPK1_DROME
AccessioniPrimary (citable) accession number: Q9W0V1
Secondary accession number(s): O62534
, Q0E8K9, Q53XF5, Q7KVE1, Q8IHG6, Q9W0V2, Q9W0V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 156 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.