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Protein

3-phosphoinositide-dependent protein kinase 1

Gene

Pdk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase required for embryonic development. Inhibits apoptosis. Acts in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and activating Akt1 and S6k. May be involved in axonal pathfinding and synaptogenesis, and in spermatogenesis.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei275ATPBy similarity1
Binding sitei331ATPBy similarity1
Active sitei370Proton acceptorPROSITE-ProRule annotation1
Binding sitei374ATP; via carbonyl oxygenBy similarity1
Binding sitei388ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 258ATPBy similarity3
Nucleotide bindingi325 – 327ATPBy similarity3

GO - Molecular functioni

  • 3-phosphoinositide-dependent protein kinase activity Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • protein kinase activator activity Source: FlyBase
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: FlyBase
  • female gamete generation Source: UniProtKB
  • insulin receptor signaling pathway Source: FlyBase
  • intracellular signal transduction Source: GO_Central
  • negative regulation of apoptotic process Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: FlyBase
  • phosphatidylinositol 3-kinase signaling Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • positive regulation of organ growth Source: FlyBase
  • positive regulation of protein kinase B signaling Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell growth Source: FlyBase
  • regulation of organ growth Source: FlyBase
  • response to ethanol Source: FlyBase
  • sex differentiation Source: UniProtKB
  • spermatogenesis Source: UniProtKB
  • synaptic growth at neuromuscular junction Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Differentiation, Growth regulation, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 1994
ReactomeiR-DME-110478 Insulin signaling pathway
R-DME-110523 TOR signaling pathway
R-DME-114604 GPVI-mediated activation cascade
R-DME-1257604 PIP3 activates AKT signaling
R-DME-165158 Activation of AKT2
R-DME-202424 Downstream TCR signaling
R-DME-2730905 Role of LAT2/NTAL/LAB on calcium mobilization
R-DME-389357 CD28 dependent PI3K/Akt signaling
R-DME-392451 G beta:gamma signalling through PI3Kgamma
R-DME-444257 RSK activation
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-5218921 VEGFR2 mediated cell proliferation
R-DME-5625740 RHO GTPases activate PKNs
SignaLinkiQ9W0V1

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoinositide-dependent protein kinase 1 (EC:2.7.11.1)
Short name:
dPDK-1
Alternative name(s):
Serine/threonine-protein kinase 61C
dSTPK61
Gene namesi
Name:Pdk1
Synonyms:Pk61C
ORF Names:CG1210
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0020386 Pdk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death during the second instar stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865041 – 8363-phosphoinositide-dependent protein kinase 1Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei641Phosphothreonine1 Publication1
Modified residuei645Phosphoserine1 Publication1
Modified residuei804Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9W0V1
PRIDEiQ9W0V1

PTM databases

iPTMnetiQ9W0V1

Expressioni

Developmental stagei

Highly expressed in Malpighian tubule primordia from stage late 11 to 13. Expressed in hindgut at stage 13. After stage 14, ubiquitously expressed at low levels.1 Publication

Gene expression databases

BgeeiFBgn0020386
ExpressionAtlasiQ9W0V1 differential
GenevisibleiQ9W0V1 DM

Interactioni

Protein-protein interaction databases

BioGridi63582, 124 interactors
DIPiDIP-20399N
IntActiQ9W0V1, 10 interactors
STRINGi7227.FBpp0305420

Structurei

3D structure databases

ProteinModelPortaliQ9W0V1
SMRiQ9W0V1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini246 – 571Protein kinasePROSITE-ProRule annotationAdd BLAST326

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 322PIF-pocketBy similarityAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 235Gln-richAdd BLAST54
Compositional biasi768 – 804Ser-richAdd BLAST37

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0592 Eukaryota
ENOG410XRT8 LUCA
GeneTreeiENSGT00550000074819
InParanoidiQ9W0V1
KOiK06276
OMAiQQHTCGC
OrthoDBiEOG091G06CX
PhylomeDBiQ9W0V1

Family and domain databases

CDDicd01262 PH_PDK1, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR033931 PDK1-typ_PH
IPR011993 PH-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF14593 PH_3, 1 hit
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q9W0V1-4) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKCKSWSNKI NNYVVRKIKS IKINGTQQQL QLPGSGASGI AAAAVITVAS
60 70 80 90 100
DCGENCSSNG TEHQQHFNIA TTTATSATEA TMPAMAKEKA SATVSLGESN
110 120 130 140 150
FRDINLKDLA VVVEAASRLH HQQNVCGCGA VSSTENNNNS RYGSSKYLTN
160 170 180 190 200
GHTSPLAAAV ASNSSSVATT PHCRMLHNCS LQQYQNDIRQ QTEILDMLRQ
210 220 230 240 250
QHQQGYQSQQ QQQQPQQQQE QQQQQEQSQQ QQQLQNPAPR RSPNDFIFGR
260 270 280 290 300
YIGEGSYSIV YLAVDIHSRR EYAIKVCEKR LILRERKQDY IKREREVMHQ
310 320 330 340 350
MTNVPGFVNL SCTFQDQRSL YFVMTYARKG DMLPYINRVG SFDVACTRHY
360 370 380 390 400
AAELLLACEH MHRRNVVHRD LKPENILLDE DMHTLIADFG SAKVMTAHER
410 420 430 440 450
ALATEHCSEQ RRSNSDEDDE DSDRLENEDE DFYDRDSEEL DDGDDEQQQE
460 470 480 490 500
EMDSPRHRQR RYNRHRKASF VGTAQYVSPE VLQNGPITPA ADLWALGCIV
510 520 530 540 550
YQMIAGLPPF RGSNDYVIFK EILDCAVDFP QGFDKDAEDL VRKLLRVDPR
560 570 580 590 600
DRLGAQDEFG YYESIRAHPF FAGIDWQTLR QQTPPPIYPY LPGVSQDEDF
610 620 630 640 650
RSSYTVPGDL EPGLDERQIS RLLSAELGVG SSVAMPVKRS TAKNSFDLND
660 670 680 690 700
AEKLQRLEQQ KTDKWHVFAD GEVILKKGFV NKRKGLFARK RMLLLTTGPR
710 720 730 740 750
LIYIDPVQMI KKGEIPWSPD LRAEYKNFKI FFVHTPNRTY YLDDPEGYAI
760 770 780 790 800
HWSEAIENMR KLAYGDPSST SAVSCSSGSS NSLAVISNSS AASSSNSPTV
810 820 830
KRSSPVNAPQ ASTASDNRTL GSTRTGTSPS KKTASK
Note: No experimental confirmation available.
Length:836
Mass (Da):94,079
Last modified:January 24, 2006 - v4
Checksum:iCA274D2265D17AE2
GO
Isoform 1 (identifier: Q9W0V1-1) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MKCKSWSNKINNYVVRKIKSIK → MNIIQ

Show »
Length:819
Mass (Da):92,028
Checksum:iEA36359C8364A3A7
GO
Isoform 2 (identifier: Q9W0V1-2) [UniParc]FASTAAdd to basket
Also known as: A, F

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Show »
Length:755
Mass (Da):85,613
Checksum:i6375A99A25089FDD
GO
Isoform 3 (identifier: Q9W0V1-3) [UniParc]FASTAAdd to basket
Also known as: B, E

The sequence of this isoform differs from the canonical sequence as follows:
     1-297: Missing.

Note: No experimental confirmation available.
Show »
Length:539
Mass (Da):60,954
Checksum:iB9B864D2527D7260
GO

Sequence cautioni

The sequence AAN71019 differs from that shown. Reason: Frameshift at positions 470 and 733.Curated
The sequence CAA69216 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200 – 201QQ → HE in CAA69216 (PubMed:10336630).Curated2
Sequence conflicti443G → R in CAA69216 (PubMed:10336630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0048971 – 297Missing in isoform 3. 1 PublicationAdd BLAST297
Alternative sequenceiVSP_0048961 – 81Missing in isoform 2. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_0170831 – 22MKCKS…IKSIK → MNIIQ in isoform 1. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07908 mRNA Translation: CAA69216.1 Different initiation.
AE014296 Genomic DNA Translation: AAF47327.3
AE014296 Genomic DNA Translation: AAF47329.2
AE014296 Genomic DNA Translation: AAF47330.3
AE014296 Genomic DNA Translation: AAF47332.1
BT004489 mRNA Translation: AAO42653.1
BT001263 mRNA Translation: AAN71019.1 Frameshift.
BT011471 mRNA Translation: AAR99129.1
RefSeqiNP_001261183.1, NM_001274254.1 [Q9W0V1-4]
NP_525121.2, NM_080382.4 [Q9W0V1-2]
NP_728470.1, NM_167789.2 [Q9W0V1-1]
NP_728471.2, NM_167790.3 [Q9W0V1-4]
NP_728472.1, NM_167791.3 [Q9W0V1-2]
NP_728473.1, NM_167792.3 [Q9W0V1-2]
NP_728474.1, NM_167793.3 [Q9W0V1-3]
NP_728475.1, NM_167794.3 [Q9W0V1-3]
NP_728476.1, NM_167795.3 [Q9W0V1-3]
UniGeneiDm.7033

Genome annotation databases

EnsemblMetazoaiFBtr0072464; FBpp0072366; FBgn0020386 [Q9W0V1-4]
FBtr0072465; FBpp0072367; FBgn0020386 [Q9W0V1-2]
FBtr0072466; FBpp0072368; FBgn0020386 [Q9W0V1-2]
FBtr0072467; FBpp0072369; FBgn0020386 [Q9W0V1-2]
FBtr0072468; FBpp0072370; FBgn0020386 [Q9W0V1-1]
FBtr0072469; FBpp0072371; FBgn0020386 [Q9W0V1-3]
FBtr0072470; FBpp0072372; FBgn0020386 [Q9W0V1-3]
FBtr0072471; FBpp0072373; FBgn0020386 [Q9W0V1-3]
FBtr0333218; FBpp0305420; FBgn0020386 [Q9W0V1-4]
GeneIDi38017
KEGGidme:Dmel_CG1210

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDPK1_DROME
AccessioniPrimary (citable) accession number: Q9W0V1
Secondary accession number(s): O62534
, Q0E8K9, Q53XF5, Q7KVE1, Q8IHG6, Q9W0V2, Q9W0V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 24, 2006
Last modified: April 25, 2018
This is version 171 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health