Q9W0G1 (PTP61_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 61F
EC=3.1.3.48

Alternative name(s):
dPTP61F

Gene names

Name:	Ptp61F
ORF Names:	CG9181

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-receptor protein tyrosine phosphatase required for maintaining Dock in its non-phosphorylated state. Ref.1 Ref.8
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.1
Subcellular location	Isoform A: Cytoplasm. Endomembrane system. Note: Associates with the membranes of the reticular network and the mitochondria. Ref.1 Isoform B: Nucleus Ref.1.
Tissue specificity	Expressed during oogenesis and embryogenesis. Isoform A and isoform B are expressed in distinct patterns. Isoform B is expressed in the mesoderm and neuroblast layer during germband extension and later in the gut epithelia. Isoform A accumulates in 16 segmentally repeated stripes in the ectoderm during germband extension. These stripes are flanked by, and adjacent to, the domains of engrailed and wingless gene expression in the anterior/posterior axis. In stage 10 embryos, isoform A colocalizes with the area lateral to the denticle belts that will give rise to naked cuticle. Isoform A is also expressed later in embryogenesis in the central nervous system. Ref.7
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from physical interaction Ref.6. Source: FlyBase cellular response to hypoxia Inferred from mutant phenotype PubMed 18653470. Source: FlyBase germ-band extension Inferred from direct assay Ref.7. Source: UniProtKB mitotic cell cycle Inferred from mutant phenotype PubMed 17306545. Source: FlyBase negative regulation of JAK-STAT cascade Inferred from mutant phenotype PubMed 16094372. Source: FlyBase negative regulation of peptidyl-tyrosine phosphorylation Inferred from mutant phenotype PubMed 17804420. Source: FlyBase regulation of protein localization Inferred from direct assay PubMed 17804420. Source: FlyBase regulation of protein stability Inferred from mutant phenotype PubMed 17804420. Source: FlyBase
Cellular_component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial envelope Inferred from direct assay Ref.1. Source: UniProtKB nucleus Inferred from direct assay Ref.1. Source: FlyBase perinuclear region of cytoplasm Inferred from direct assay PubMed 17804420. Source: FlyBase
Molecular_function	protein tyrosine phosphatase activity Inferred from direct assay Ref.1. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dock	Q24218	6	EBI-74714,EBI-74727

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A Ref.1 (identifier: Q9W0G1-1)

Also known as: Cytoplasmic; DPTP61Fm;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B Ref.1 (identifier: Q9W0G1-2)

Also known as: Nuclear; DPTP61Fn;

The sequence of this isoform differs from the canonical sequence as follows:
     525-548: SLLTYIAAGVVVGVICAYAYTKLG → RGNRLKKSKTK

Isoform C Ref.3 (identifier: Q9W0G1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MSEQKTSGSGSAAAARLQIEAEYKDKGPQWHRFY → MTKFAQRSKKTCEIRGTMDVCVCVCVSMW
     525-548: SLLTYIAAGVVVGVICAYAYTKLG → RGNRLKKSKTK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Tyrosine-protein phosphatase non-receptor type 61F		PRO_0000094853
Regions
	Domain	33 – 296	264	Tyrosine-protein phosphatase
	Region	237 – 243	7	Substrate binding By similarity
Sites
	Active site	237	1	Phosphocysteine intermediate By similarity UniProtKB P18031
	Binding site	203	1	Substrate By similarity
	Binding site	281	1	Substrate By similarity
Amino acid modifications
	Modified residue	83	1	Phosphoserine Ref.9
	Modified residue	86	1	Phosphotyrosine Ref.9
Natural variations
	Alternative sequence	1 – 34	34	MSEQK…WHRFY → MTKFAQRSKKTCEIRGTMDV CVCVCVSMW in isoform C. Ref.3		VSP_050733
	Alternative sequence	525 – 548	24	SLLTY…YTKLG → RGNRLKKSKTK in isoform B and isoform C. Ref.1		VSP_050259
Experimental info
	Sequence conflict	274	1	S → T Ref.1
	Sequence conflict	274	1	S → T Ref.2
	Sequence conflict	382	1	I → F in AAA75348. Ref.1
	Sequence conflict	382	1	I → F in AAA75349. Ref.1
	Sequence conflict	382	1	I → F in AAA75338. Ref.1
	Sequence conflict	382	1	I → F in AAA75339. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (Cytoplasmic) (DPTP61Fm) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C6AF630E2DE3F11C	FASTA	548	62,105
	10 20 30 40 50 60 MSEQKTSGSG SAAAARLQIE AEYKDKGPQW HRFYKEICET CDREAKEKQF STSESERHTN 70 80 90 100 110 120 RGLNRYRDVN PYDHSRIVLK RGSVDYINAN LVQLERAERQ YILTQGPLVD TVGHFWLMVW 130 140 150 160 170 180 EQKSRAVLML NKLMEKKQIK CHLYWPNEMG ADKALKLPHV KLTVELVRLE TYQNFVRRWF 190 200 210 220 230 240 KLTDLETQQS REVMQFHYTT WPDFGIPSSP NAFLKFLQQV RDSGCLSRDV GPAVVHCSAG 250 260 270 280 290 300 IGRSGTFCLV DCCLVLIDKY GECNVSKVLC ELRSYRMGLI QTADQLDFSY QAIIEGIKKL 310 320 330 340 350 360 HDPTFLDAEE PLISNDTETH TLDELPPPLP PRVQSLNLPL APNSGGILSL NMRAAQANGA 370 380 390 400 410 420 ESIGKELSKD ALNNFINQHD MIHDAEVADS RPLPPLPVRA FNDSDSDEDY LLDDDDEDDT 430 440 450 460 470 480 DEDEEYETIN EHDADPVNGH VPATTQPHAD DVNANNEKPA VPVDEQHKAN GIDPIPGQLP 490 500 510 520 530 540 ASPENELKRR KRNEYQASLE QKVNDMKRKQ RENEDKQLAA KKRRSLLTYI AAGVVVGVIC AYAYTKLG
	Isoform B (Nuclear) (DPTP61Fn) [UniParc]. Checksum: D80C8CD1AC99AF60	FASTA	535	60,974
	10 20 30 40 50 60 MSEQKTSGSG SAAAARLQIE AEYKDKGPQW HRFYKEICET CDREAKEKQF STSESERHTN 70 80 90 100 110 120 RGLNRYRDVN PYDHSRIVLK RGSVDYINAN LVQLERAERQ YILTQGPLVD TVGHFWLMVW 130 140 150 160 170 180 EQKSRAVLML NKLMEKKQIK CHLYWPNEMG ADKALKLPHV KLTVELVRLE TYQNFVRRWF 190 200 210 220 230 240 KLTDLETQQS REVMQFHYTT WPDFGIPSSP NAFLKFLQQV RDSGCLSRDV GPAVVHCSAG 250 260 270 280 290 300 IGRSGTFCLV DCCLVLIDKY GECNVSKVLC ELRSYRMGLI QTADQLDFSY QAIIEGIKKL 310 320 330 340 350 360 HDPTFLDAEE PLISNDTETH TLDELPPPLP PRVQSLNLPL APNSGGILSL NMRAAQANGA 370 380 390 400 410 420 ESIGKELSKD ALNNFINQHD MIHDAEVADS RPLPPLPVRA FNDSDSDEDY LLDDDDEDDT 430 440 450 460 470 480 DEDEEYETIN EHDADPVNGH VPATTQPHAD DVNANNEKPA VPVDEQHKAN GIDPIPGQLP 490 500 510 520 530 ASPENELKRR KRNEYQASLE QKVNDMKRKQ RENEDKQLAA KKRRRGNRLK KSKTK
	Isoform C [UniParc]. Checksum: 1EF64FCDF6AA177E	FASTA	530	60,487
	10 20 30 40 50 60 MTKFAQRSKK TCEIRGTMDV CVCVCVSMWK EICETCDREA KEKQFSTSES ERHTNRGLNR 70 80 90 100 110 120 YRDVNPYDHS RIVLKRGSVD YINANLVQLE RAERQYILTQ GPLVDTVGHF WLMVWEQKSR 130 140 150 160 170 180 AVLMLNKLME KKQIKCHLYW PNEMGADKAL KLPHVKLTVE LVRLETYQNF VRRWFKLTDL 190 200 210 220 230 240 ETQQSREVMQ FHYTTWPDFG IPSSPNAFLK FLQQVRDSGC LSRDVGPAVV HCSAGIGRSG 250 260 270 280 290 300 TFCLVDCCLV LIDKYGECNV SKVLCELRSY RMGLIQTADQ LDFSYQAIIE GIKKLHDPTF 310 320 330 340 350 360 LDAEEPLISN DTETHTLDEL PPPLPPRVQS LNLPLAPNSG GILSLNMRAA QANGAESIGK 370 380 390 400 410 420 ELSKDALNNF INQHDMIHDA EVADSRPLPP LPVRAFNDSD SDEDYLLDDD DEDDTDEDEE 430 440 450 460 470 480 YETINEHDAD PVNGHVPATT QPHADDVNAN NEKPAVPVDE QHKANGIDPI PGQLPASPEN 490 500 510 520 530 ELKRRKRNEY QASLEQKVND MKRKQRENED KQLAAKKRRR GNRLKKSKTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative splicing gives rise to a nuclear protein tyrosine phosphatase in Drosophila." McLaughlin S., Dixon J.E. J. Biol. Chem. 268:6839-6842(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS A AND B), FUNCTION, SUBCELLULAR LOCATION. Strain: Canton-S. Tissue: Head.
[2]	"Identification of a Drosophila protein tyrosine phosphatase expressed in a pattern of segmental stripes in the germ band extended embryo." Kung T.Y., Tian S.S., Zinn K. Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley. Tissue: Larva and Pupae.
[6]	"A Drosophila protein-tyrosine phosphatase associates with an adapter protein required for axonal guidance." Clemens J.C., Ursuliak Z., Clemens K.K., Price J.V., Dixon J.E. J. Biol. Chem. 271:17002-17005(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DOCK.
[7]	"Differential accumulation of DPTP61F alternative transcripts: regulation of a protein tyrosine phosphatase by segmentation genes." Ursuliak Z., Clemens J.C., Dixon J.E., Price J.V. Mech. Dev. 65:19-30(1997) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Use of double-stranded RNA-mediated interference to determine the substrates of protein tyrosine kinases and phosphatases." Muda M., Worby C.A., Simonson-Leff N., Clemens J.C., Dixon J.E. Biochem. J. 366:73-77(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83 AND TYR-86, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11250, L11249 Genomic DNA. Translation: AAA75348.1. L11251, L11249, L11250 Genomic DNA. Translation: AAA75349.1. L11252 mRNA. Translation: AAA75338.1. L11253 mRNA. Translation: AAA75339.1. L14849 mRNA. Translation: AAA28748.1. AE014296 Genomic DNA. Translation: AAF47486.1. AE014296 Genomic DNA. Translation: AAF47487.1. AE014296 Genomic DNA. Translation: AAN11475.1. AY069713 mRNA. Translation: AAL39858.1.
PIR	A46101. B46101.
RefSeq	NP_476687.1. NM_057339.4. NP_476688.1. NM_057340.3. NP_728600.1. NM_167874.2.
UniGene	Dm.1991.
3D structure databases
ProteinModelPortal	Q9W0G1.
SMR	Q9W0G1. Positions 13-352.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9W0G1. 5 interactions.
MINT	MINT-823894.
Proteomic databases
PaxDb	Q9W0G1.
PRIDE	Q9W0G1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072718; FBpp0072602; FBgn0003138.
GeneID	38160.
KEGG	dme:Dmel_CG9181.
Organism-specific databases
CTD	38160.
FlyBase	FBgn0003138. Ptp61F.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104092.
InParanoid	Q9W0G1.
KO	K05696.
OMA	FCLVDCC.
OrthoDB	EOG43J9MB.
PhylomeDB	Q9W0G1.
Gene expression databases
Bgee	Q9W0G1.
GermOnline	CG9181. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	38160.
NextBio	807270.

Entry information

Entry name

PTP61_DROME

Accession

Primary (citable) accession number: Q9W0G1
Secondary accession number(s): Q27932, Q8SZY3, Q9W0G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project