Reviewed, UniProtKB/Swiss-Prot Q9W032 (SGT1_DROME)

Names and origin

Protein names

Recommended name:
    Protein SGT1 homolog ecdysoneless

Gene names

Name:	ecd
ORF Names:	CG5714

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required in both the follicle cells and the germline for oocyte development. Ref.4
Subcellular location	Cytoplasm. Ref.4
Tissue specificity	Expressed in the ecdysone-producing larval ring gland, nervous system, imaginal disks and gonads. Ref.4
Developmental stage	Expressed both maternally and zygotically throughout development. Ref.4
Sequence similarities	Belongs to the SGT1 family.

Ontologies

Keywords
Biological process	Differentiation Oogenesis
Cellular component	Cytoplasm
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Golgi organization Inferred from mutant phenotype. Source: FlyBase cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW chitin-based embryonic cuticle biosynthetic process Inferred from mutant phenotype. Source: FlyBase ecdysone biosynthetic process Traceable author statement. Source: FlyBase germ-band shortening Inferred from mutant phenotype. Source: FlyBase head involution Inferred from mutant phenotype. Source: FlyBase instar larval development Ref.4 Inferred from mutant phenotype. Source: FlyBase lymph gland development Inferred from mutant phenotype. Source: FlyBase molting cycle, chitin-based cuticle Ref.4 Inferred from mutant phenotype. Source: FlyBase oogenesis Ref.4 Inferred from mutant phenotype. Source: FlyBase progression of morphogenetic furrow during compound eye morphogenesis Inferred from mutant phenotype. Source: FlyBase response to symbiont Inferred from genetic interaction. Source: FlyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Protein SGT1 homolog ecdysoneless		PRO_0000220846
Experimental info
	Mutagenesis	656	1	P → S: Temperature-sensitive mutation; disrupts production of the steroid hormone ecdysone, causes developmental and reproductive defects. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9W032-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D59B5C943E352F30	FASTA	684	77,908
	10 20 30 40 50 60 MSKIPGSNLE FVREEDFVEY YIFPKIPDNV QDEGALRKLM LQIRNEISAI VREKSLERSY 70 80 90 100 110 120 LWHKDEFQLQ VRLGGAEERL LNEETNPEEE AELGDLPPHF HGVTHYGDNI SDEWFVVYLL 130 140 150 160 170 180 TEITRARGDC IARVSDSDGE FLLIEAADAL PDWASPETCE QRVYLVGGHL QLLQNSAASS 190 200 210 220 230 240 QDKPLTMAMA VQRIRMNPTL YRCSQEIQSC IDARLKEYQI AQPHFSIHRQ VLELPHSAAQ 250 260 270 280 290 300 LLKQKPRLLS SAVRAFCERD SLDIKALRTM RYFPPEATRV RTNVRFTRCL YAMLSHQQYL 310 320 330 340 350 360 PEKRLGWHLT DPVSEPERYK EQLLGLKLAS GLEILATQAK RVEGQQLEDL PAWRSYLRSL 370 380 390 400 410 420 LSKGYFRDNI EGSAEYQELL NKAKVYFRGN QERFRTASRA GAEILDLLLH PAEAASEELR 430 440 450 460 470 480 DEENNLQPSD SDEWLNISAE DLDSMLQDRY GPKKLYKPNG QMNAEEFTKQ LAEFLDRQSN 490 500 510 520 530 540 YEGIEHRGLE EPELDSDDDE PPPQANGSTG LTAKVKKNPS MRKACQRNSV IQPEEPDSTH 550 560 570 580 590 600 VRNFLDFVIP EDNWDSTSEM SDYADEDDME SNLNALSGGG SVFPLDRQIQ SYMEQMDREL 610 620 630 640 650 660 AQTSVGKSFH GKKKTAPQAD EDDFDDIEDF EPININVNTL RNMMDSYQSQ VGGAGPVSNL 670 680 FSAMGVGMSA VEDKEQKDIS ESAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[4]	"Cell-autonomous roles of the ecdysoneless gene in Drosophila development and oogenesis." Gaziova I., Bonnette P.C., Henrich V.C., Jindra M. Development 131:2715-2725(2004) [PubMed: 15128659] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PRO-656.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF47628.1. AY051483 mRNA. Translation: AAK92907.1.
RefSeq	NP_647707.1.
UniGene	Dm.4588
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9W032. 1 interaction.
Proteomic databases
PRIDE	Q9W032.
Genome annotation databases
Ensembl	FBgn0000543. Drosophila melanogaster. [Contig view]
GeneID	38291.
KEGG	dme:Dmel_CG5714.
NMPDR	fig|7227.3.peg.7728.
Organism-specific databases
FlyBase	FBgn0000543. ecd.
Phylogenomic databases
HOGENOM	Q9W032.
OMA	Q9W032. LRACRVF.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014817-MON.
Gene expression databases
ArrayExpress	Q9W032.
GermOnline	CG5714. Drosophila melanogaster.
Family and domain databases
InterPro	IPR010770. SGT1. [Graphical view]
PANTHER	PTHR13060. SGT1. 1 hit.
Pfam	PF07093. SGT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	807890.

Entry information

Entry name

SGT1_DROME

Accession

Primary (citable) accession number: Q9W032

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project