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Reviewed, UniProtKB/Swiss-Prot Q9VZU2 (HHAT_DROME)

Last modified January 19, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein-cysteine N-palmitoyltransferase Rasp
    EC=2.3.1.-
Alternative name(s):
    Protein skinny hedgehog
    Protein sightless
    Protein central missing
Gene names
Name: rasp
Synonyms: cmn, sit, ski
ORF Names: CG11495
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required in hedgehog (hh) expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. Acts within the secretory pathway to catalyze N-terminal palmitoylation of Hh; this lipid modification is required for the embryonic and larval patterning activities of the Hh signal. Not required for Wg signaling. Ref.1 Ref.2 Ref.3 Ref.7

Subcellular location

Membrane; Multi-pass membrane protein.

Developmental stage

Expressed both maternally and zygotically. Ref.3

Sequence similarities

Belongs to the membrane-bound acyltransferase family. HHAT subfamily.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Arp5Q9VEC31EBI-117067,EBI-85467

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Protein-cysteine N-palmitoyltransferase Rasp
PRO_0000213136

Regions

Transmembrane15 – 3521 Potential
Transmembrane73 – 9321 Potential
Transmembrane105 – 12521 Potential
Transmembrane134 – 15421 Potential
Transmembrane206 – 22621 Potential
Transmembrane243 – 26321 Potential
Transmembrane293 – 31321 Potential
Transmembrane372 – 39221 Potential
Transmembrane429 – 44921 Potential
Transmembrane461 – 48121 Potential

Sites

Active site3811 Potential

Experimental info

Sequence conflict911G → V Ref.1
Sequence conflict911G → V Ref.6

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Sequences

Sequence LengthMass (Da)Tools
Q9VZU2-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 4498DC306976F2A2

FASTA50058,106
        10         20         30         40         50         60 
MSRLPDRSLL TRCEIFVYFG VYIAYIVVGL YKIYGLRDHI VKEAKFQFPE GWSLYPFSQR 

        70         80         90        100        110        120 
RRDDSNDELE NFGDFIVSFW PFYLLHVAVQ GFIRWKRPRL QCLGFIGVCA LALSVNLDWS 

       130        140        150        160        170        180 
SMVLLVTLIA SYYIVSLLSL KFLVWLLSAG WILCINVMQK NVWWTDRVGY TEYVLVIVTM 

       190        200        210        220        230        240 
SWSVLRGCSY SLSKIGAKQE DLTRYSLVQY LGYAMYFPCL TYGPIISYQR FAARREDEVQ 

       250        260        270        280        290        300 
NWLGFVGGVL RSAIWWLVMQ CALHYFYIHY MSRDVRMVEM MDSVFWQHSA GYFMGQFFFL 

       310        320        330        340        350        360 
YYVVTYGLGI AFAVQDGIPA PNRPRCIGRI HFYSDMWKYF DEGLYEFLFQ NIYAELCGKR 

       370        380        390        400        410        420 
SSAAAKFGAT ALTFAFVFVW HGCYTYVLIW SILNFLCLAA EKVFKTFTAM PEYQRWTQRH 

       430        440        450        460        470        480 
LGAVGAQRLY AMLATQLFIP AAFSNVYFIG GQEIGDFLMR GAYLSGVGNY VALCFCSYCF 

       490        500 
FQCSELLLTK SDGRSKTKTF

« Hide

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References

« Hide 'large scale' references
[1]"Sightless has homology to transmembrane acyltransferases and is required to generate active Hedgehog protein."
Lee J.D., Treisman J.E.
Curr. Biol. 11:1147-1152(2001) [PubMed: 11509241] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Embryo.
[2]"Distinct roles of Central missing and Dispatched in sending the Hedgehog signal."
Amanai K., Jiang J.
Development 128:5119-5127(2001) [PubMed: 11748147] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]"Skinny hedgehog, an acyltransferase required for palmitoylation and activity of the hedgehog signal."
Chamoun Z., Mann R.K., Nellen D., von Kessler D.P., Bellotto M., Beachy P.A., Basler K.
Science 293:2080-2084(2001) [PubMed: 11486055] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[4]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[5]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[7]"Rasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling."
Micchelli C.A., The I., Selva E., Mogila V., Perrimon N.
Development 129:843-851(2002) [PubMed: 11861468] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF393157 mRNA. Translation: AAK73748.1.
AF398410 mRNA. Translation: AAK97480.1.
AE014296 Genomic DNA. Translation: AAF47725.1.
AY119202 mRNA. Translation: AAM51062.1.
RefSeqNP_523898.1.
UniGeneDm.2681

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ9VZU2. 3 interactions.
STRINGQ9VZU2.

Proteomic databases

PRIDEQ9VZU2.

Genome annotation databases

EnsemblFBtr0073029; FBpp0072893; FBgn0024194; Drosophila melanogaster. [Genome view]
GeneID44098.
KEGGdme:Dmel_CG11495.
NMPDRfig|7227.3.peg.7868.

Organism-specific databases

CTD44098.
FlyBaseFBgn0024194. rasp.

Phylogenomic databases

eggNOGinNOG09424.
InParanoidQ9VZU2.
OMALHYFYIH.
OrthoDBEOG9XSKX3.
PhylomeDBQ9VZU2.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-014942-MONOMER.

Gene expression databases

GermOnlineCG11495. Drosophila melanogaster.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio836757.

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Entry information

Entry nameHHAT_DROME
AccessionPrimary (citable) accession number: Q9VZU2
Secondary accession number(s): Q95VY0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: May 1, 2000
Last modified: January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents