Reviewed, UniProtKB/Swiss-Prot Q9VZS6 (NSMA_DROME)

Names and origin

Protein names

Recommended name:
    Putative neutral sphingomyelinase
    EC=3.1.4.12

Gene names

ORF Names:

CG12034

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
jp	Q9VLA8	1	EBI-125601,EBI-141560
Kr-h2	Q9V447	1	EBI-125601,EBI-135893

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Putative neutral sphingomyelinase		PRO_0000075690
Regions
	Transmembrane	362 – 384	23	Potential
	Transmembrane	391 – 413	23	Potential
Sites
	Active site	264	1	Proton acceptor By similarity
	Metal binding	46	1	Magnesium By similarity
	Site	177	1	Important for substrate recognition By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9VZS6-1 [UniParc]. Last modified June 20, 2003. Version 2. Checksum: 7167808A4D9ABBE0	FASTA	442	49,643
	10 20 30 40 50 60 MLLLELNILT LNIWGIPYVS SDRRPRIDAI CKELASGKYD IVSLQEVWAQ EDSELLQKGT 70 80 90 100 110 120 EAVLPHSHYF HSGVMGAGLL VLSKYPILGT LFHAWSVNGY FHRIQHADWF GGKGVGLCRI 130 140 150 160 170 180 LVGGQMVHLY NAHLHAEYDN ANDEYKTHRV IQAFDTAQFI EATRGNSALQ ILAGDLNAQP 190 200 210 220 230 240 QDISYKVLLY TSKMLDSCDS DSFRTNECEH NSYTSKQARE RNPLGIRIDH IFVRGGDHVN 250 260 270 280 290 300 AEIAEYKLPF PERVPGEKFS FSDHEAVMAK LKLFKLEPRS EEPVATIEVN CLVEDGETCS 310 320 330 340 350 360 VREVGAGDAL TGEDDQSSQH QPEIQCNGSS TSIQSMPAAR TAALLEALAL CDASLLQLNT 370 380 390 400 410 420 DRILYYSAAT FLFVLLVLLV EFTAPVGMRT IFLLLKFIVF GVILFCVFMA SIWNYMERNG 430 440 VLQGKKSMEV MLHHAQKYEY FY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF47741.2. AY051714 mRNA. Translation: AAK93138.1.
RefSeq	NP_647790.1.
UniGene	Dm.4499
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:18690N.
IntAct	Q9VZS6. 3 interactions.
STRING	Q9VZS6.
Genome annotation databases
Ensembl	FBtr0073043; FBpp0072907; FBgn0035421; Drosophila melanogaster. [Genome view]
GeneID	38396.
KEGG	dme:Dmel_CG12034.
NMPDR	fig|7227.3.peg.7896.
UCSC	CG12034-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0035421. CG12034.
Phylogenomic databases
OMA	IRIDHIF.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014969-MON.
BRENDA	3.1.4.12. 48.
Gene expression databases
Bgee	Q9VZS6.
GermOnline	CG12034. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	808410.

Entry information

Entry name

NSMA_DROME

Accession

Primary (citable) accession number: Q9VZS6
Secondary accession number(s): Q961B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project