Q9VZM5 (PIGB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 3
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase III
Short name=GPI-MT-III

Gene names

ORF Names:

CG12006

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 22 family. PIGB subfamily.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	mannosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VZM5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q9VZM5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     475-504: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	GPI mannosyltransferase 3		PRO_0000246255
Regions
	Transmembrane	3 – 25	23	Helical; Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	195 – 215	21	Helical; Potential
	Transmembrane	246 – 266	21	Helical; Potential
	Transmembrane	275 – 295	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
Amino acid modifications
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	456	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	475 – 504	30	Missing in isoform B.		VSP_019841

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 5A94054C6E9DB239	FASTA	561	65,007
	10 20 30 40 50 60 MKLIYVFLLI LAVRLASVFV VQTYYVPDEY WQSLEVAHKL TFGYGYLTWE WVQGIRSYVY 70 80 90 100 110 120 PLLIAGLYKI LALLQLDSAH LLVVLPRIVQ ALLSAYSDYR FFVWTGKRKW ALFLILVPWF 130 140 150 160 170 180 WFYTGSRTLA NTLEASLTTI ALSYFPWYGE STAYLWPAAI CCFLRPTAAV IWLPLSLYHL 190 200 210 220 230 240 RRSRQNVLEL ILKRFVLIGL LVAGLGIAID TYWHGQLIVT PYEFLKYNIF NNIGSFYGSH 250 260 270 280 290 300 PWHWYFSVGL PTVLGINTLP FIFGVMETVK KSEKYPVSKQ LLITIFLTLV VLSAVEHKEF 310 320 330 340 350 360 RFVSPLLPLC LYVITDALSR WSIRASSTML WTTALVILVG NVMPAWYLST VHQKGPIELM 370 380 390 400 410 420 PKLREIAREY RDEREHQANI LFLMPCHSTP YYSHIHQNVT MRFLTCEPNL EKKEQYKDEA 430 440 450 460 470 480 DRFFEDPVHW INSHIPMHPL TALPTHVVLF DPLAENISVF LRNYRLLHRI EHAEVTRLEG 490 500 510 520 530 540 SQALVDQWSE ALGAQSPNLA SLLQNRQSRT GRSILVYQRL KKGEENAFNR GPDSGQHEPD 550 560 VHDHPPLEDL VLANENENLF N
	Isoform B [UniParc]. Checksum: 0F7DC736AE714DF9	FASTA	531	61,842
	10 20 30 40 50 60 MKLIYVFLLI LAVRLASVFV VQTYYVPDEY WQSLEVAHKL TFGYGYLTWE WVQGIRSYVY 70 80 90 100 110 120 PLLIAGLYKI LALLQLDSAH LLVVLPRIVQ ALLSAYSDYR FFVWTGKRKW ALFLILVPWF 130 140 150 160 170 180 WFYTGSRTLA NTLEASLTTI ALSYFPWYGE STAYLWPAAI CCFLRPTAAV IWLPLSLYHL 190 200 210 220 230 240 RRSRQNVLEL ILKRFVLIGL LVAGLGIAID TYWHGQLIVT PYEFLKYNIF NNIGSFYGSH 250 260 270 280 290 300 PWHWYFSVGL PTVLGINTLP FIFGVMETVK KSEKYPVSKQ LLITIFLTLV VLSAVEHKEF 310 320 330 340 350 360 RFVSPLLPLC LYVITDALSR WSIRASSTML WTTALVILVG NVMPAWYLST VHQKGPIELM 370 380 390 400 410 420 PKLREIAREY RDEREHQANI LFLMPCHSTP YYSHIHQNVT MRFLTCEPNL EKKEQYKDEA 430 440 450 460 470 480 DRFFEDPVHW INSHIPMHPL TALPTHVVLF DPLAENISVF LRNYRLLHRI EHAENRQSRT 490 500 510 520 530 GRSILVYQRL KKGEENAFNR GPDSGQHEPD VHDHPPLEDL VLANENENLF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014296 Genomic DNA. Translation: AAF47795.2. AE014296 Genomic DNA. Translation: AAS64961.1. BT003240 mRNA. Translation: AAO24997.1.
RefSeq	NP_647832.1. NM_139575.2. NP_995991.1. NM_206269.2.
UniGene	Dm.11589.
3D structure databases
ProteinModelPortal	Q9VZM5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VZM5.
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073149; FBpp0073008; FBgn0035464.
GeneID	38446.
KEGG	dme:Dmel_CG12006.
NMPDR	fig|7227.3.peg.7983.
UCSC	CG12006-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0035464. CG12006.
Phylogenomic databases
eggNOG	inNOG06692.
GeneTree	EMGT00050000010281.
InParanoid	Q9VZM5.
OMA	HWINSHI.
OrthoDB	EOG4DJHBR.
PhylomeDB	Q9VZM5.
Gene expression databases
Bgee	Q9VZM5.
GermOnline	CG12006. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005599. GPI_mannosylTrfase. [Graphical view]
KO	K05286.
PANTHER	PTHR22760. Alg9_trans. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	808712.

Entry information

Entry name

PIGB_DROME

Accession

Primary (citable) accession number: Q9VZM5
Secondary accession number(s): Q7KV63

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project