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Protein

GTP-binding nuclear protein Ran

Gene

Ran

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis (By similarity). GTP-bound Ran modulates both spindle and nuclear envelope assembly, supporting a role during mitosis (PubMed:12121620).By similarity1 Publication

Cofactori

Mg2+By similarityNote: Mg2+ interacts primarily with the phosphate groups of the bound guanine nucleotide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68GTP; via amide nitrogenBy similarity1
Sitei69Essential for GTP hydrolysisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 25GTPBy similarity8
Nucleotide bindingi36 – 42GTPBy similarity7
Nucleotide bindingi122 – 125GTPBy similarity4
Nucleotide bindingi150 – 152GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of mitotic spindle orientation Source: FlyBase
  • intracellular protein transport Source: UniProtKB
  • meiotic spindle organization Source: FlyBase
  • protein export from nucleus Source: FlyBase
  • protein import into nucleus Source: GO_Central
  • regulation of mitotic cell cycle Source: FlyBase
  • regulation of protein localization Source: FlyBase
  • ribosomal subunit export from nucleus Source: GO_Central

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Protein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
R-DME-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein Ran
Alternative name(s):
Ras-related nuclear protein
Gene namesi
Name:RanImported
Synonyms:Ran10A
ORF Names:CG1404Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0020255 Ran

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002087101 – 216GTP-binding nuclear protein RanAdd BLAST216

Proteomic databases

PaxDbiQ9VZ23
PRIDEiQ9VZ23

Expressioni

Gene expression databases

BgeeiFBgn0020255
ExpressionAtlasiQ9VZ23 baseline and differential
GenevisibleiQ9VZ23 DM

Interactioni

Subunit structurei

Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity). Interacts with tamo (PubMed:12653959).By similarity1 Publication

Protein-protein interaction databases

BioGridi68814, 12 interactors
IntActiQ9VZ23, 7 interactors
MINTiQ9VZ23
STRINGi7227.FBpp0073328

Structurei

3D structure databases

ProteinModelPortaliQ9VZ23
SMRiQ9VZ23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

eggNOGiKOG0096 Eukaryota
ENOG410XNRS LUCA
GeneTreeiENSGT00840000129836
InParanoidiQ9VZ23
KOiK07936
OMAiYNVWDTA
OrthoDBiEOG091G0L3X
PhylomeDBiQ9VZ23

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR002041 Ran_GTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PANTHERiPTHR24071 PTHR24071, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PRINTSiPR00627 GTPRANTC4
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51418 RAN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VZ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEGQDIPT FKCVLVGDGG TGKTTFVKRH MTGEFEKKYV ATLGVEVHPL
60 70 80 90 100
IFHTNRGAIR FNVWDTAGQE KFGGLRDGYY IQGQCAVIMF DVTSRVTYKN
110 120 130 140 150
VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS
160 170 180 190 200
AKSNYNFEKP FLWLARKLVG DPNLEFVAMP ALLPPEVKMD KDWQAQIERD
210
LQEAQATALP DEDEEL
Length:216
Mass (Da):24,708
Last modified:May 1, 2000 - v1
Checksum:i95916B5D4C3413F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90 – 93FDVT → VDGN in AAF30287 (Ref. 2) Curated4
Sequence conflicti163 – 164WL → CW in AAF30287 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233584 mRNA Translation: AAF60289.1
AF220950 mRNA Translation: AAF30287.1
AE014298 Genomic DNA Translation: AAF48008.1
AY061398 mRNA Translation: AAL28946.1
AY070533 mRNA Translation: AAL48004.1
BT003574 mRNA Translation: AAO39578.1
AF233585 mRNA Translation: AAF60290.1
RefSeqiNP_001285111.1, NM_001298182.1
NP_001285112.1, NM_001298183.1
NP_651969.1, NM_143712.4
NP_727499.1, NM_167272.3
UniGeneiDm.6755

Genome annotation databases

EnsemblMetazoaiFBtr0073471; FBpp0073327; FBgn0020255
FBtr0073472; FBpp0073328; FBgn0020255
FBtr0340164; FBpp0309150; FBgn0020255
FBtr0340165; FBpp0309151; FBgn0020255
GeneIDi44072
KEGGidme:Dmel_CG1404
UCSCiCG1404-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiRAN_DROME
AccessioniPrimary (citable) accession number: Q9VZ23
Secondary accession number(s): Q9NH14, Q9NHN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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