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Protein

Alpha-(1,6)-fucosyltransferase

Gene

FucT6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. The addition is prevented if the GlcNAc residue is already fucosylated.1 Publication

Catalytic activityi

GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.1 Publication

Cofactori

Mn2+By similarity, Mg2+By similarityNote: May also use Ca2+. The enzyme has substantial activity without divalent cations.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-975578. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-(1,6)-fucosyltransferase (EC:2.4.1.681 Publication)
Short name:
Alpha1-6FucT
Alternative name(s):
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
GDP-fucose--glycoprotein fucosyltransferase
Glycoprotein 6-alpha-L-fucosyltransferase
Gene namesi
Name:FucT6
ORF Names:CG2448
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030327. FucT6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicSequence analysisAdd BLAST17
Transmembranei18 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini39 – 619LumenalSequence analysisAdd BLAST581

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805301 – 619Alpha-(1,6)-fucosyltransferaseAdd BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi253 ↔ 315By similarity
Disulfide bondi261 ↔ 279By similarity
Disulfide bondi267 ↔ 271By similarity
Disulfide bondi511 ↔ 518By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9VYV5.
PRIDEiQ9VYV5.

Expressioni

Gene expression databases

BgeeiFBgn0030327.
GenevisibleiQ9VYV5. DM.

Interactioni

Protein-protein interaction databases

BioGridi58529. 3 interactors.
DIPiDIP-21409N.
IntActiQ9VYV5. 2 interactors.
MINTiMINT-900962.
STRINGi7227.FBpp0073457.

Structurei

3D structure databases

ProteinModelPortaliQ9VYV5.
SMRiQ9VYV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini255 – 539GT23PROSITE-ProRule annotationAdd BLAST285
Domaini548 – 609SH3By similarityAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni411 – 412Important for donor substrate bindingPROSITE-ProRule annotation2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi345 – 351SH3-bindingSequence analysis7

Sequence similaritiesi

Belongs to the glycosyltransferase 23 family.PROSITE-ProRule annotation
Contains 1 GT23 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
GeneTreeiENSGT00530000063737.
InParanoidiQ9VYV5.
KOiK00717.
OMAiNARKLVC.
OrthoDBiEOG091G05GD.
PhylomeDBiQ9VYV5.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VYV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLVRQLFGA SANSWARALI IFVLAWIGLV YVFVVKLTNT QGQQAAGESE
60 70 80 90 100
LNARRISQAL QMLEHTRQRN EELKQLIDEL MSDQLDKQSA MKLVQRLEND
110 120 130 140 150
ALNPKLAPEV AGPEPESMFE SAPADLRGWN NVAEGAPNDL EAGVPDHGEF
160 170 180 190 200
EPSLEYEFTR RRIQTNIGEI WNFFSSELGK VRKAVAAGHA SADLEESINQ
210 220 230 240 250
VLLQGAEHKR SLLSDMERMR QSDGYEAWRH KEARDLSDLV QRRLHHLQNP
260 270 280 290 300
SDCQNARKLV CKLNKGCGYG CQLHHVVYCF IVAYATERTL ILKSRGWRYH
310 320 330 340 350
KGGWEEVFQP VSNSCHDAGT ANTYNWPGKP NTQVLVLPII DSLMPRPPYL
360 370 380 390 400
PLAVPEDLAP RLKRLHGDPI VWWVGQFLKY LLRPQPTTRD FLTSGMRNLG
410 420 430 440 450
WERPIVGVHV RRTDKVGTEA ACHSVEEYMT YVEDYYRTLE VNGSTVARRI
460 470 480 490 500
FLASDDAQVI EEARRKYPQY QIIGDPEVAR MASVSTRYTD TALNGIILDI
510 520 530 540 550
HLLSMSDHLV CTFSSQVCRV AYEIMQTMYP DAAHRFKSLD DIYYYGGQNA
560 570 580 590 600
HNRRVVIAHK PRTHEDLQLR VGDLVSVAGN HWDGNSKGKN TRTNQGGLFP
610
SFKVEEKVDT AKLPLYAGI
Length:619
Mass (Da):70,206
Last modified:May 1, 2000 - v1
Checksum:i691BFD9B5C6557DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441264 mRNA. Translation: AAN63649.1.
AE014298 Genomic DNA. Translation: AAF48079.1.
AY051451 mRNA. Translation: AAK92875.1.
RefSeqiNP_572740.1. NM_132512.4.
UniGeneiDm.4605.

Genome annotation databases

EnsemblMetazoaiFBtr0073620; FBpp0073457; FBgn0030327.
GeneIDi32122.
KEGGidme:Dmel_CG2448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441264 mRNA. Translation: AAN63649.1.
AE014298 Genomic DNA. Translation: AAF48079.1.
AY051451 mRNA. Translation: AAK92875.1.
RefSeqiNP_572740.1. NM_132512.4.
UniGeneiDm.4605.

3D structure databases

ProteinModelPortaliQ9VYV5.
SMRiQ9VYV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58529. 3 interactors.
DIPiDIP-21409N.
IntActiQ9VYV5. 2 interactors.
MINTiMINT-900962.
STRINGi7227.FBpp0073457.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

Proteomic databases

PaxDbiQ9VYV5.
PRIDEiQ9VYV5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073620; FBpp0073457; FBgn0030327.
GeneIDi32122.
KEGGidme:Dmel_CG2448.

Organism-specific databases

CTDi32122.
FlyBaseiFBgn0030327. FucT6.

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
GeneTreeiENSGT00530000063737.
InParanoidiQ9VYV5.
KOiK00717.
OMAiNARKLVC.
OrthoDBiEOG091G05GD.
PhylomeDBiQ9VYV5.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-DME-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSiFucT6. fly.
GenomeRNAii32122.
PROiQ9VYV5.

Gene expression databases

BgeeiFBgn0030327.
GenevisibleiQ9VYV5. DM.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUT8_DROME
AccessioniPrimary (citable) accession number: Q9VYV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.