Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

Usp7

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves ubiquitin fusion protein substrates.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei250 – 2501NucleophilePROSITE-ProRule annotation
Active sitei490 – 4901Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: GO_Central

GO - Biological processi

  1. chromatin silencing Source: FlyBase
  2. histone deubiquitination Source: FlyBase
  3. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  4. protein deubiquitination Source: UniProtKB
  5. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ9VYQ8.

Protein family/group databases

MEROPSiC19.A52.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Short name:
Deubiquitinating enzyme 7
Gene namesi
Name:Usp7
ORF Names:CG1490
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030366. Usp7.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: UniProtKB
  3. polytene chromosome Source: FlyBase
  4. protein complex Source: FlyBase
  5. transcriptionally silent chromatin Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11291129Ubiquitin carboxyl-terminal hydrolase 7PRO_0000268011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1117 – 11171Phosphoserine1 Publication

Post-translational modificationi

Polyneddylated.By similarity
Polyubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9VYQ8.
PRIDEiQ9VYQ8.

Expressioni

Gene expression databases

BgeeiQ9VYQ8.

Interactioni

Protein-protein interaction databases

BioGridi58574. 10 interactions.
IntActiQ9VYQ8. 2 interactions.
MINTiMINT-805769.

Structurei

3D structure databases

ProteinModelPortaliQ9VYQ8.
SMRiQ9VYQ8. Positions 98-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 222122MATHPROSITE-ProRule annotationAdd
BLAST
Domaini241 – 548308USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 MATH domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
InParanoidiQ9VYQ8.
KOiK11838.
OMAiEMEEDEC.
OrthoDBiEOG75B84G.
PhylomeDBiQ9VYQ8.

Family and domain databases

InterProiIPR002083. MATH.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR024729. USP7_ICP0-binding_dom.
IPR029346. USP_C.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF14533. USP7_C2. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTiSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
PROSITEiPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VYQ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIETDQSIE AMDTQDTQEV EILTSDLQQT QQQRNSPPQL PKFKNLIQPQ
60 70 80 90 100
LHAVGAVTQL PSENGNMPPQ QLLADSSSTS FGDGEAMGID DESKEDQFRS
110 120 130 140 150
ETTFSFTVEN VVQLKSQRLS PPVYVRMLPW RIMVIPNDRA LGFFLQCNGE
160 170 180 190 200
NDSPTWSCNA IAELRLKCHK PDAQPFTRAR IKHLFYSKEN DYGYSNFITW
210 220 230 240 250
QELKDSEKSY VHNNSITLEV HVVADAPHGV LWDSKKHTGY VGLKNQGATC
260 270 280 290 300
YMNSLLQTLY FTNSLRLSVY RIPTEADDSS KSVGLSLQRV FHELQFGDRP
310 320 330 340 350
VGTKKLTKSF GWETLDSFMQ HDVQEFLRVL LDKLESKMKG TILEGTIPGL
360 370 380 390 400
FEGKMSSYIK CKNVDYNSTR YETFYDIQLN IKDKKNIYES FQDYVAPETL
410 420 430 440 450
EGDNKYDAGV HGLQEASKGV IFTSFPPVLH LHLMRFQYDP VTDSSIKYND
460 470 480 490 500
RFEFYEHINL DRYLAESENT LADYVLHAVL VHSGDNHGGH YVVFINPKAD
510 520 530 540 550
GRWFKFDDDV VSSCRKQEAI EQNYGGMDDE ISFHAKCSNA YMLVYIRQSE
560 570 580 590 600
LDRVLGDITE SEISSDLVER LDLEKRIEMA RRKERGEANT YVSVHVILEE
610 620 630 640 650
NFEEQHKRRL FDLEKVHPRV FRIKQNQTVD ELVDLFVRGF GVSRQRMRMW
660 670 680 690 700
NLCTAQTQKF SHFDFVAEGS RTIEQISTSQ KPWVIWLQLA WTDVPGPLPP
710 720 730 740 750
FNPKTESLLF LKYYDPRNKR LNYIGCTQQP HTRRLIDLVP DVNSKLGFEP
760 770 780 790 800
DTELTIYDEY ADKKLVNLNE PIESALFIPQ DHLQGHILIF ERENVDAKLD
810 820 830 840 850
LPTVGDYFLD LVYRIEIIFS DKCNPNEPDF TLELSNRYNY DQLANAVAER
860 870 880 890 900
LNTDPQKLQF FMCINNYKET AGNAVPYTFK GTIKDLVSYT KQSSTKRIFY
910 920 930 940 950
QRLSLSIHEL DNKKQFKCVW VSSDLKDEKE LVLYPNKNDT VKGLLEEAAK
960 970 980 990 1000
KISFAENSRR KLRLLKISNH KIVAVCKDDI PLDTLLKSNE SITTAQGAQK
1010 1020 1030 1040 1050
TFRIEEVPAE DMQLAENEFL IPVAHFSKEL YNSFGIPFLT KARQGEPYGA
1060 1070 1080 1090 1100
LKQRIQRRLN VQDKEWENYK FCVISMGHNA DVNDNTPVDL EVYRSWTSGQ
1110 1120
LPFFGLDHIN KSRKRSSLNF SEKAIKIYN
Length:1,129
Mass (Da):130,446
Last modified:May 1, 2000 - v1
Checksum:i41F7D1B654579258
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48134.1.
BT044531 mRNA. Translation: ACH95305.1.
RefSeqiNP_572779.2. NM_132551.3.
UniGeneiDm.3592.

Genome annotation databases

EnsemblMetazoaiFBtr0073641; FBpp0073474; FBgn0030366.
GeneIDi32169.
KEGGidme:Dmel_CG1490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48134.1.
BT044531 mRNA. Translation: ACH95305.1.
RefSeqiNP_572779.2. NM_132551.3.
UniGeneiDm.3592.

3D structure databases

ProteinModelPortaliQ9VYQ8.
SMRiQ9VYQ8. Positions 98-581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58574. 10 interactions.
IntActiQ9VYQ8. 2 interactions.
MINTiMINT-805769.

Protein family/group databases

MEROPSiC19.A52.

Proteomic databases

PaxDbiQ9VYQ8.
PRIDEiQ9VYQ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073641; FBpp0073474; FBgn0030366.
GeneIDi32169.
KEGGidme:Dmel_CG1490.

Organism-specific databases

CTDi7874.
FlyBaseiFBgn0030366. Usp7.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
InParanoidiQ9VYQ8.
KOiK11838.
OMAiEMEEDEC.
OrthoDBiEOG75B84G.
PhylomeDBiQ9VYQ8.

Enzyme and pathway databases

SignaLinkiQ9VYQ8.

Miscellaneous databases

ChiTaRSiUsp7. fly.
GenomeRNAii32169.
NextBioi777232.
PROiQ9VYQ8.

Gene expression databases

BgeeiQ9VYQ8.

Family and domain databases

InterProiIPR002083. MATH.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR024729. USP7_ICP0-binding_dom.
IPR029346. USP_C.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF14533. USP7_C2. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTiSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
PROSITEiPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1117, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiUBP7_DROME
AccessioniPrimary (citable) accession number: Q9VYQ8
Secondary accession number(s): B5RJQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.