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Protein

26S proteasome non-ATPase regulatory subunit 5

Gene

CG12096

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome.By similarity

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • proteasome regulatory particle assembly Source: GO_Central

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 5By similarity
Alternative name(s):
26S proteasome subunit S5BBy similarity
Short name:
dS5b1 Publication
Gene namesi
ORF Names:CG12096
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030457. CG12096.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44E → A: Abolishes interaction with PI31. 1 Publication1
Mutagenesisi45R → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi46L → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi165P → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi172D → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi261V → T: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi263R → A: Abolishes interaction with PI31. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004248941 – 50626S proteasome non-ATPase regulatory subunit 5Add BLAST506

Proteomic databases

PaxDbiQ9VYG1.
PRIDEiQ9VYG1.

Expressioni

Gene expression databases

BgeeiFBgn0030457.
ExpressionAtlasiQ9VYG1. differential.
GenevisibleiQ9VYG1. DM.

Interactioni

Subunit structurei

Interacts with PI31; this interaction is increased by PI31 ADP-ribosylation. Interacts with Rpt2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi58654. 3 interactors.
IntActiQ9VYG1. 3 interactors.
MINTiMINT-828383.
STRINGi7227.FBpp0073558.

Structurei

3D structure databases

ProteinModelPortaliQ9VYG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proteasome subunit S5B/HSM3 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG4413. Eukaryota.
ENOG410XTFA. LUCA.
InParanoidiQ9VYG1.
KOiK06692.
OMAiDSPQQVC.
OrthoDBiEOG091G09IW.
PhylomeDBiQ9VYG1.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR019538. 26S_Psome_nonATP_su5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
PfamiView protein in Pfam
PF10508. Proteasom_PSMB. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9VYG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMEDNWWVT KLKALESKPQ RLDALTAMNS AIAKEAALPR QIIERLLTTD
60 70 80 90 100
QLYDCANPAA DSHVPKDQAV DVTLELLCHC LDQLAMDTAD EQLSSLLRRG
110 120 130 140 150
LTHSNPALRA QVLASLFKKL LRQLTVGQVL TLPNNELIFL ILDELKQPDT
160 170 180 190 200
QSTSLAINIL SIVLPQRISN ADVQAKLVQL LKQNEIVRCR AYELAVVLAK
210 220 230 240 250
KSATLLSDVT FILDAALSEL DNDDVLLQAS VMELLVPLAE QNHGLSYMER
260 270 280 290 300
RRVLDIISYR VQRVEEHPLD ALLVPSIMKF FGKISVYQPL KIIGGYPHML
310 320 330 340 350
ACLFMQLQSE DESILPTAMD TLANLATTPQ GKILLNMHFS GAMEKSFKKY
360 370 380 390 400
GSHTKKLSAH IKKRLLNSLD VIYDFKTPPA TEIINIGKNW YECFAGGAHA
410 420 430 440 450
NIIMDLINTP FPDLQMAALS FLKTICKYNW GIVALKNTGG AVEFLLSRQK
460 470 480 490 500
DLHRDIKYMK WQIMEILSAS AEFSPTETIR FTAYVNEGPY HVQADLDVAT

EPQGNA
Length:506
Mass (Da):56,668
Last modified:July 5, 2004 - v3
Checksum:iFDD4CA3103FDAD33
GO

Sequence cautioni

The sequence AAL39843 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326A → T in ABY20534 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48236.3.
AY069698 mRNA. Translation: AAL39843.1. Different initiation.
BT031293 mRNA. Translation: ABY20534.1.
BT133216 mRNA. Translation: AFA28457.1.
RefSeqiNP_001285190.1. NM_001298261.1.
NP_001285191.1. NM_001298262.1.
NP_572862.2. NM_132634.4.
UniGeneiDm.1998.

Genome annotation databases

EnsemblMetazoaiFBtr0073727; FBpp0073558; FBgn0030457.
FBtr0340298; FBpp0309259; FBgn0030457.
FBtr0340299; FBpp0309260; FBgn0030457.
GeneIDi32269.
KEGGidme:Dmel_CG12096.
UCSCiCG12096-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48236.3.
AY069698 mRNA. Translation: AAL39843.1. Different initiation.
BT031293 mRNA. Translation: ABY20534.1.
BT133216 mRNA. Translation: AFA28457.1.
RefSeqiNP_001285190.1. NM_001298261.1.
NP_001285191.1. NM_001298262.1.
NP_572862.2. NM_132634.4.
UniGeneiDm.1998.

3D structure databases

ProteinModelPortaliQ9VYG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58654. 3 interactors.
IntActiQ9VYG1. 3 interactors.
MINTiMINT-828383.
STRINGi7227.FBpp0073558.

Proteomic databases

PaxDbiQ9VYG1.
PRIDEiQ9VYG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073727; FBpp0073558; FBgn0030457.
FBtr0340298; FBpp0309259; FBgn0030457.
FBtr0340299; FBpp0309260; FBgn0030457.
GeneIDi32269.
KEGGidme:Dmel_CG12096.
UCSCiCG12096-RA. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0030457. CG12096.

Phylogenomic databases

eggNOGiKOG4413. Eukaryota.
ENOG410XTFA. LUCA.
InParanoidiQ9VYG1.
KOiK06692.
OMAiDSPQQVC.
OrthoDBiEOG091G09IW.
PhylomeDBiQ9VYG1.

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii32269.
PROiPR:Q9VYG1.

Gene expression databases

BgeeiFBgn0030457.
ExpressionAtlasiQ9VYG1. differential.
GenevisibleiQ9VYG1. DM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR019538. 26S_Psome_nonATP_su5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
PfamiView protein in Pfam
PF10508. Proteasom_PSMB. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD5_DROME
AccessioniPrimary (citable) accession number: Q9VYG1
Secondary accession number(s): A9UNC9, Q7K0M3, Q8SZZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: July 5, 2004
Last modified: June 7, 2017
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.