Reviewed, UniProtKB/Swiss-Prot Q9VYD1 (C1716_DROME)

Names and origin

Protein names

Recommended name:
    Probable histone-lysine N-methyltransferase CG1716
    EC=2.1.1.43

Gene names

Name:	Set2
ORF Names:	CG1716

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable histone methyltransferase. Histone methylation gives specific tags for epigenetic transcriptional activation or repression By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 2 A.T hook DNA-binding domains. Contains 1 AWS domain. Contains 1 post-SET domain. Contains 1 SET domain. Contains 1 WW domain.
Sequence caution	The sequence AAF48273.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAK84931.1 differs from that shown. Reason: Frameshift at position 759.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Chromatin regulator Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ecdysone receptor-mediated signaling pathway Inferred from genetic interaction. Source: FlyBase histone H3-K36 methylation Inferred from mutant phenotype. Source: FlyBase instar larval development Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW wing disc development Inferred from mutant phenotype. Source: FlyBase
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone methyltransferase activity (H3-K36 specific) Inferred from mutant phenotype. Source: FlyBase phosphoprotein binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VQA2-1	1	EBI-179700,EBI-198744

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2313	2313	Probable histone-lysine N-methyltransferase CG1716		PRO_0000259523
Regions
	Domain	1307 – 1360	54	AWS
	Domain	1361 – 1483	123	SET
	Domain	1486 – 1502	17	Post-SET
	Domain	1963 – 1996	34	WW
	DNA binding	17 – 29	13	A.T hook 1
	DNA binding	197 – 209	13	A.T hook 2
	Compositional bias	326 – 672	347	Glu-rich
	Compositional bias	675 – 898	224	Lys-rich
	Compositional bias	983 – 1008	26	Ser-rich
Amino acid modifications
	Modified residue	404	1	Phosphothreonine Ref.5
	Modified residue	786	1	Phosphoserine Ref.5
	Modified residue	788	1	Phosphoserine Ref.5
	Modified residue	2130	1	Phosphoserine Ref.5
	Modified residue	2131	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	490	1	F → S in AAK84931. Ref.4
	Sequence conflict	500	1	C → R in AAK84931. Ref.4
	Sequence conflict	509	1	M → L in AAK84931. Ref.4
	Sequence conflict	700	1	T → A in AAK84931. Ref.4
	Sequence conflict	710	1	N → K in AAK84931. Ref.4
	Sequence conflict	737	1	K → R in AAK84931. Ref.4
	Sequence conflict	2148	1	E → A in AAK84931. Ref.4
	Sequence conflict	2179	1	T → S in AAK84931. Ref.4
	Sequence conflict	2202	1	S → N in AAK84931. Ref.4
	Sequence conflict	2215	1	T → S in AAK84931. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VYD1-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 811F133520284E2F	FASTA	2,313	256,791
	10 20 30 40 50 60 MEESGPPSNP SPVASRGRGR GRPPKVALSA LGNTPPHINP SLKHADAEAS PTAPEDQDSG 70 80 90 100 110 120 QSECRRSSRK KIIKFDVRDL LNKNRKAHKI QIEARIDSNP STGHSQSGTT AASTSMSTAT 130 140 150 160 170 180 ASAASASSAA TVSRLFSMFE MSHQSLPPPP PPPTALEIFA KPRPTQSLIV AQVTSEPSAV 190 200 210 220 230 240 GGAHPVQTMA GLPPVTPRKR GRPRKSQLAD AAIIPTVIVP SCSDSDTNST STTTSNMSSD 250 260 270 280 290 300 SGELPGFPIQ KPKSKLRVSL KRLKLGGRLE SSDSGNSPSS SSPEVEPPAL QDENAMDERP 310 320 330 340 350 360 KQEQNLSRMV DAEENSDSDS QIIFIEIETE SPKGEEEQEE GRPVEVEPQD LIDIDMELAK 370 380 390 400 410 420 QEPTPDPEED LDEIMVEVLS GPPSLWSADD EAEEEEDATV QRATPPGKEP AADSCSSAPR 430 440 450 460 470 480 RSRRSAPLSG SSRQGKTLEE TFAEIAAESS KQILEAEESQ DQEEQHILID LIEDTLKSDN 490 500 510 520 530 540 IAASLNKDIF EPKVETKATC GEVVPRPEMV TEDVYITEGI AATLEKSAVV TKPTTEMIAE 550 560 570 580 590 600 TKLSDEVVIE PPLKDESDPK QTEVELPESK PAVNIPKSER ILSAEVETTS SPLVPPECCT 610 620 630 640 650 660 LESVSGPVLL ETSLSTEEKS NENVETTPLK TEAAKEDSPP AAPEEEASNS SEEPNFLLED 670 680 690 700 710 720 YESNQEQVAE DEMMKCNNQK GQKQTPLPEM KEPEKPVAET VSKKEKAMEN PARSSPAIVD 730 740 750 760 770 780 KKVRAGEMEK KVVKSTKGTV PEKKMDSKKS CAAVTPAKQK ESGKSAKEAI LKKETEKEKS 790 800 810 820 830 840 SAKLDSSSPN TLDKKGKDTA QWSPQLQTLP KSSTKPPQES APSVISKTTS NQPAPKEEQH 850 860 870 880 890 900 AAKKGLSDNS PPSVLKAKEK AVSGFVECDA MFKAMDLANA QLRLDEKNKK KLKKVPTKVE 910 920 930 940 950 960 APPKVEPPTA VPVPGQKKSL SGKTSLRRNT VYEDSPNLER NSSPSSDSAQ ANTSAGKLKP 970 980 990 1000 1010 1020 SKVKKKINPR RSTICEAAKD LRSSSSSSTP TREVAASSPV STSSDSSSKR NGSKRTTSDL 1030 1040 1050 1060 1070 1080 DGGSKLDQRR YTICEDRQPE TAIPVPLTKR RFSMHPKASA NPLHDTLLQT AGKKRGRKEG 1090 1100 1110 1120 1130 1140 KESLSRQNSL DSSSSASQGA PKKKALKSAE ILSAALLETE SSESTSSGSK MSRWDVQTSP 1150 1160 1170 1180 1190 1200 ELEAANPFGD IAKFIEDGVN LLKRDKVDED QRKEGQDEVK READPEEDEF AQRVANMETP 1210 1220 1230 1240 1250 1260 ATTPTPSPTQ SNPEDSASTT TVLKELETGG GVRRSHRIKQ KPQGPRASQG RGVASVALAP 1270 1280 1290 1300 1310 1320 ISMDEQLAEL ANIEAINEQF LRSEGLNTFQ LLKENFYRCA RQVSQENAEM QCDCFLTGDE 1330 1340 1350 1360 1370 1380 EAQGHLSCGA GCINRMLMIE CGPLCSNGAR CTNKRFQQHQ CWPCRVFRTE KKGCGITAEL 1390 1400 1410 1420 1430 1440 LIPPGEFIME YVGEVIDSEE FERRQHLYSK DRNRHYYFMA LRGEAVIDAT SKGNISRYIN 1450 1460 1470 1480 1490 1500 HSCDPNAETQ KWTVNGELRI GFFSVKPIQP GEEITFDYQY LRYGRDAQRC YCEAANCRGW 1510 1520 1530 1540 1550 1560 IGGEPDSDEG EQLDEESDSD AEMDEEELEA EPEEGQPRKS AKAKAKSKLK AKLPLATGRK 1570 1580 1590 1600 1610 1620 RKEQTKPKDR EYKAGRWLKP SATGSSSSAE KPPKKPKVNK FQAMLEDPDV VEELSLLRRG 1630 1640 1650 1660 1670 1680 GLKNQQDTLR FSRCLVRAKL LKTRLALLRV LTHGELPCRR LFLDYHGLRL LHAWISENGN 1690 1700 1710 1720 1730 1740 DDQLREALLD TLESLPIPNR TMLSDSRVYQ SVQLWSNSLE QQLAVVPQEK QAALHKRMVA 1750 1760 1770 1780 1790 1800 LLQKWQALPE IFRIPKRERI EQMKEHEREA DRQQKHVHAS TALEDQRERE SSNDRFRQDR 1810 1820 1830 1840 1850 1860 FRRDTTSSRI GKPIRMSGNN TICTITTQQK GSNGAPDGMT RNDNRRRSDI GPPSEQRRTL 1870 1880 1890 1900 1910 1920 SKELRRSLFE RKVALDEAER RVCTEDRLEH ELRCEFFGAD INTDPKQLPF YQKTDTNEWF 1930 1940 1950 1960 1970 1980 NSDDVPVPAP PRTELLTKAL LSPDIDVGQG ATDVEYKLPP GVDPLPPAWN WQVTSDGDIY 1990 2000 2010 2020 2030 2040 YYNLRERISQ WEPPSPEQRL QTLLEENTTQ QPLHELQIDP AVLENELIQV DTDYVGSLSA 2050 2060 2070 2080 2090 2100 KSLAQYIEAK VRERRDLRRS KLVSIRLISP RRDEDRLYNQ LESRKYKENK EKIRRRKELY 2110 2120 2130 2140 2150 2160 RRRKIEVLPD AVDEIPVPGK ALPIQPYLFS SDEEETKVAA IEQPAAEEEQ DSLNMAPSTS 2170 2180 2190 2200 2210 2220 HAAMAALGKA VAQPTGLGTV GKRKLPMPPS VTVKKHRQEQ RSKKVKSSQS PLTATSAREA 2230 2240 2250 2260 2270 2280 HEKFRFEISG HVANFLRPYR KESCTLGRIT SDEDYKFLVN RLSYHITTKE MRYCEVSGNP 2290 2300 2310 LSCTESVKHK SYDFINQYMR QKGPVYKKPA END

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 478-2313. Strain: Berkeley. Tissue: Embryo.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-404; SER-786; SER-788; SER-2130 AND SER-2131, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF48273.2. Sequence problems. BT025042 mRNA. Translation: ABE73213.1. AY050232 mRNA. Translation: AAK84931.1. Frameshift.
RefSeq	NP_001162740.1. NP_572888.2.
3D structure databases
HSSP	HSSP built from PDB template 1ZCN based on UniProtKB Q13526.
SMR	Q9VYD1. Positions 1332-1502, 1963-2009.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VYD1. 1 interaction.
STRING	Q9VYD1.
Genome annotation databases
Ensembl	FBtr0073779; FBpp0073610; FBgn0030486; Drosophila melanogaster. [Genome view]
GeneID	32301.
KEGG	dme:Dmel_CG1716.
UCSC	CG1716-RA. d. melanogaster.
Organism-specific databases
CTD	32301.
FlyBase	FBgn0030486. Set2.
Phylogenomic databases
eggNOG	inNOG04113.
InParanoid	Q9VYD1.
OrthoDB	EOG9FJ8GM.
Enzyme and pathway databases
BRENDA	2.1.1.43. 48.
Gene expression databases
ArrayExpress	Q9VYD1.
Bgee	Q9VYD1.
GermOnline	CG1716. Drosophila melanogaster.
Family and domain databases
InterPro	IPR017956. AT_hook_DNA-bd_motif. IPR006560. AWS. IPR006210. EGF-like. IPR003616. Post-SET_dom. IPR001214. SET_dom. IPR001202. WW_Rsp5_WWP. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00384. AT_hook. 2 hits. SM00570. AWS. 1 hit. SM00181. EGF. 1 hit. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. SM00456. WW. 1 hit. [Graphical view]
PROSITE	PS51215. AWS. 1 hit. PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. PS01159. WW_DOMAIN_1. 1 hit. PS50020. WW_DOMAIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	777831.

Entry information

Entry name

C1716_DROME

Accession

Primary (citable) accession number: Q9VYD1
Secondary accession number(s): Q1RL18, Q961S6, Q9VYD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	February 9, 2010
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project