Q9VYB7 (TPST_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-tyrosine sulfotransferase
EC=2.8.2.20

Alternative name(s):
Transport and Golgi organization protein 13
Short name=Tango-13
Tyrosylprotein sulfotransferase
Short name=TPST

Gene names

Name:	Tango13
ORF Names:	CG32632

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides By similarity. Has a role in protein secretion. Ref.6
Catalytic activity	3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.6.
Sequence similarities	Belongs to the protein sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein secretion Inferred from mutant phenotype Ref.6. Source: FlyBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein-tyrosine sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VYB7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9VYB7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     337-346: GKPDAWVQDN → VKGQSNAVGE
     347-499: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Protein-tyrosine sulfotransferase		PRO_0000189833
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 499	469	Lumenal Potential
	Compositional bias	374 – 395	22	Asn-rich
	Compositional bias	401 – 435	35	Gln-rich
	Compositional bias	474 – 490	17	Asn-rich
Amino acid modifications
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	380	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	337 – 346	10	GKPDAWVQDN → VKGQSNAVGE in isoform B.		VSP_018999
	Alternative sequence	347 – 499	153	Missing in isoform B.		VSP_019000

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 5412380E9FAD8DC2	FASTA	499	58,050
	10 20 30 40 50 60 MRLPYRNKKV TLWVLFGIIV ITMFLFKFTE LRPTCLFKVD AANELSSQMV RVEKYLTDDN 70 80 90 100 110 120 QRVYSYNREM PLIFIGGVPR SGTTLMRAML DAHPDVRCGQ ETRVIPRILQ LRSHWLKSEK 130 140 150 160 170 180 ESLRLQEAGI TKEVMNSAIA QFCLEIIAKH GEPAPRLCNK DPLTLKMGSY VIELFPNAKF 190 200 210 220 230 240 LFMVRDGRAT VHSIISRKVT ITGFDLSSYR QCMQKWNHAI EVMHEQCRDI GKDRCMMVYY 250 260 270 280 290 300 EQLVLHPEEW MRKILKFLDV PWNDAVLHHE EFINKPNGVP LSKVERSSDQ VIKPVNLEAM 310 320 330 340 350 360 SKWVGQIPGD VVRDMADIAP MLSVLGYDPY ANPPDYGKPD AWVQDNTSKL KANRMLWESK 370 380 390 400 410 420 AKQVLQMSSS EDDNTNTIIN NSNNKDNNNN QYTINKIIPE QHSRQRQHVQ QQHLQQQQQQ 430 440 450 460 470 480 HLQQQQHQRQ QQQQQREEES ESEREAEPDR EQQLLHQKPK DVITIKQLPL AGSNNNNINN 490 NINNNNNNNN IMEDPMADT
	Isoform B [UniParc]. Checksum: E252A9861215841C	FASTA	346	39,863
	10 20 30 40 50 60 MRLPYRNKKV TLWVLFGIIV ITMFLFKFTE LRPTCLFKVD AANELSSQMV RVEKYLTDDN 70 80 90 100 110 120 QRVYSYNREM PLIFIGGVPR SGTTLMRAML DAHPDVRCGQ ETRVIPRILQ LRSHWLKSEK 130 140 150 160 170 180 ESLRLQEAGI TKEVMNSAIA QFCLEIIAKH GEPAPRLCNK DPLTLKMGSY VIELFPNAKF 190 200 210 220 230 240 LFMVRDGRAT VHSIISRKVT ITGFDLSSYR QCMQKWNHAI EVMHEQCRDI GKDRCMMVYY 250 260 270 280 290 300 EQLVLHPEEW MRKILKFLDV PWNDAVLHHE EFINKPNGVP LSKVERSSDQ VIKPVNLEAM 310 320 330 340 SKWVGQIPGD VVRDMADIAP MLSVLGYDPY ANPPDYVKGQ SNAVGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosylprotein sulfotransferase from Drosophila melanogaster." Moore K.L., Sato-Tomomori C. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Larva and Pupae.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Larva and Pupae.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Ovary.
[6]	"Functional genomics reveals genes involved in protein secretion and Golgi organization." Bard F., Casano L., Mallabiabarrena A., Wallace E., Saito K., Kitayama H., Guizzunti G., Hu Y., Wendler F., Dasgupta R., Perrimon N., Malhotra V. Nature 439:604-607(2006) [PubMed: 16452979] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY124548 mRNA. Translation: AAM94031.1. AE014298 Genomic DNA. Translation: AAF48286.2. AY119073 mRNA. Translation: AAM50933.1. BT021950 mRNA. Translation: AAX51655.1.
RefSeq	NP_001096973.1. NM_001103503.1. NP_727717.1. NM_167374.2.
UniGene	Dm.17452.
3D structure databases
ProteinModelPortal	Q9VYB7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VYB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073803; FBpp0290842; FBgn0086674.
GeneID	32312.
KEGG	dme:Dmel_CG32632.
NMPDR	fig|7227.3.peg.17717.
Organism-specific databases
CTD	32312.
FlyBase	FBgn0086674. Tango13.
Phylogenomic databases
eggNOG	inNOG05161.
GeneTree	EMGT00050000006091.
InParanoid	Q9VYB7.
OMA	SSQMVRV.
OrthoDB	EOG412JN4.
PhylomeDB	Q9VYB7.
Gene expression databases
Bgee	Q9VYB7.
GermOnline	CG32632. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000863. Sulfotransferase_dom. [Graphical view]
KO	K01021.
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	777876.

Entry information

Entry name

TPST_DROME

Accession

Primary (citable) accession number: Q9VYB7
Secondary accession number(s): Q58CJ7, Q8MM59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 16, 2004
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project