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Q9VYA0 (NADE_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamine-dependent NAD(+) synthetase
EC=6.3.5.1
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
Gene names
ORF Names:CG9940
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›

Protein attributes

Sequence length787 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.

Sequence similarities

In the C-terminal section; belongs to the NAD synthetase family.

Contains 1 CN hydrolase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 787787Probable glutamine-dependent NAD(+) synthetase
PRO_0000152249

Regions

Domain5 – 294290CN hydrolase
Nucleotide binding355 – 3628ATP By similarity
Region325 – 787463Ligase

Sites

Active site3571 By similarity

Amino acid modifications

Modified residue7031Phosphoserine Ref.4

Experimental info

Sequence conflict2471A → V in AAN71229. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9VYA0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 0B2B6106E327F0C5

FASTA78787,615
        10         20         30         40         50         60 
MGRKVTVAVS TLNQWALDFE GNMVRILQSI LEAKDMGASY RTGPELEVCG YSCEDHFREP 

        70         80         90        100        110        120 
DTFLHSWEVL LEVMMSPMCE NMLVDVGMPV MHRNVAYNCR VAFFNRQILL IRPKMAMCDD 

       130        140        150        160        170        180 
GNYRESRWFT AWTKALQTEE YVLPRMIAQH TGQQTVPFGD AVIATRDTCL GYEICEELWN 

       190        200        210        220        230        240 
VRSKHIEMSL AGVELIVNSS GSYMELRKAH ITSDLIRNAS FKAGGAYLFS NLRGCDGQRV 

       250        260        270        280        290        300 
YFNGCSAIAL NGEILARSQQ FALQDVEVTL ATIDLEEIRA YRVSLRSRCT AAASAAEYPR 

       310        320        330        340        350        360 
IHCDFEMSTH SDIFKTSTPP LNWPMHTPEE EIALGPACWL WDYLRRSGQG GFFLPLSGGV 

       370        380        390        400        410        420 
DSSSSATIVH SMCRQIVQAV QQGDAQVLHD IRQLLADSDY TPDNAAGLCN RLLVTCYMGS 

       430        440        450        460        470        480 
VNSSKETRRR AAQLANQLGS YHIEISIDSA VNALLSIFNA VTGLTPRFRT QGGCARQNLA 

       490        500        510        520        530        540 
LQNMQSRIRM VLAYIFAQLT LWVRNRPGGL LVLGSANVDE SLRGYLTKYD CSSADINPIG 

       550        560        570        580        590        600 
GISKMDLRRF LTYAKDKFNL PVLESIIDAP PTAELEPLQE NGELQQTDEA DMGMTYAELS 

       610        620        630        640        650        660 
QFGRLRKQSF CGPYSMFCHL VATWKSDLSP KEVAEKVKHF FLCYAINRHK MTVLTPSVHA 

       670        680        690        700        710        720 
ESYSPDDNRF DHRPFLYRPN WSWQFKAIDD EAEKLQPIYT PSSAQLRPSS EDLLISTQRS 

       730        740        750        760        770        780 
SHLDDSKHSS PLSSASASAS IDVGISTAAV PLPGAAAPGG LSKKPSGYSK VHVNVLGKIK 


DRTGIPV

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[4]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-703, MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014298 Genomic DNA. Translation: AAF48303.1.
AE014298 Genomic DNA. Translation: AAN09333.1.
BT001474 mRNA. Translation: AAN71229.1.
RefSeqNP_572913.1. NM_132685.1.
NP_727727.1. NM_167378.1.
UniGeneDm.4895.

3D structure databases

ProteinModelPortalQ9VYA0.
SMRQ9VYA0. Positions 5-285, 324-653.
ModBaseSearch...

Proteomic databases

PaxDbQ9VYA0.
PRIDEQ9VYA0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0073844; FBpp0073675; FBgn0030512.
FBtr0073845; FBpp0073676; FBgn0030512.
GeneID32328.
KEGGdme:Dmel_CG9940.
UCSCCG9940-RA. d. melanogaster.

Organism-specific databases

FlyBaseFBgn0030512. CG9940.

Phylogenomic databases

eggNOGCOG0388.
GeneTreeENSGT00390000010152.
InParanoidQ9VYA0.
KOK01950.
OMADFALSCH.
OrthoDBEOG4H189T.
PhylomeDBQ9VYA0.

Enzyme and pathway databases

UniPathwayUPA00253; UER00334.

Gene expression databases

BgeeQ9VYA0.
GermOnlineCG9940. Drosophila melanogaster.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF006630. NADS_GAT. 1 hit.
SUPFAMSSF56317. Ntlse/CNhydtse. 1 hit.
TIGRFAMsTIGR00552. nadE. 1 hit.
PROSITEPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi32328.
NextBio777963.

Entry information

Entry nameNADE_DROME
AccessionPrimary (citable) accession number: Q9VYA0
Secondary accession number(s): A4V4E7, Q8IH17
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents