Reviewed, UniProtKB/Swiss-Prot Q9VXT7 (ASGL1_DROME)

Names and origin

Protein names

Recommended name:
    Probable L-asparaginase CG7860
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase CG7860

Gene names

ORF Names:

CG7860

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts in asparagine catabolism By similarity.
Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine catabolic process via L-aspartate Inferred from sequence or structural similarity. Source: UniProtKB salivary gland cell autophagic cell death Inferred from expression pattern. Source: FlyBase
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EG:BACR37P7.5	Q9V3G9	1	EBI-175067,EBI-162998

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Probable L-asparaginase CG7860		PRO_0000305210
Sites
	Active site	188	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9VXT7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B4BA4AC9A3521CBB	FASTA	332	34,861
	10 20 30 40 50 60 MPRPVLLIHG GAGDISDSRI AGKFAGIKQA LRSAWGLLSP DNGSGGGSAL DAVEAAVRSM 70 80 90 100 110 120 ELDENFNAGY GSCLNTSGQV ELEASLMEGR DLRAGCITLL RDVMHPITVA RRLMEKQRHT 130 140 150 160 170 180 FLGGAAAQEL ALATGSERLQ PGALVTEGAR LTLKEFEDQV AQGKDPFFAR TELTDDKPVP 190 200 210 220 230 240 KTDPSGETVG AVAMDASGQI VVGTSTGGIT GKWPGRIGDT PILGSGTYAD NCRGGVSTTG 250 260 270 280 290 300 HGETLMRYNL AQRILSAMEY QGLSAQAAAD KECREMTKRL GGTGGAIVVG HSGDLGISFT 310 320 330 SRRMAWGYVQ DGTIFYGIEG QVVHQEPFTL ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF48471.1. AY061292 mRNA. Translation: AAL28840.1.
RefSeq	NP_573039.1.
UniGene	Dm.29220
3D structure databases
HSSP	HSSP built from PDB template 1K2X based on UniProtKB P37595.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VXT7. 3 interactions.
STRING	Q9VXT7.
Protein family/group databases
MEROPS	T02.002.
Proteomic databases
PRIDE	Q9VXT7.
Genome annotation databases
Ensembl	FBtr0074045; FBpp0073861; FBgn0030653; Drosophila melanogaster. [Genome view]
GeneID	32488.
KEGG	dme:Dmel_CG7860.
NMPDR	fig|7227.3.peg.17958.
UCSC	CG7860-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0030653. CG7860.
Phylogenomic databases
HOGENOM	Q9VXT7.
OMA	WVAKWTS.
Enzyme and pathway databases
BRENDA	3.5.1.1. 48.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	778726.

Entry information

Entry name

ASGL1_DROME

Accession

Primary (citable) accession number: Q9VXT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project