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Protein

Eukaryotic translation initiation factor 5

Gene

eIF5

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPSequence analysis8

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: FlyBase

GO - Biological processi

  • translational initiation Source: FlyBase

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:eIF5
ORF Names:CG9177
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030719. eIF5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125201 – 464Eukaryotic translation initiation factor 5Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphoserine1 Publication1
Modified residuei412Phosphoserine1 Publication1
Modified residuei413Phosphoserine1 Publication1
Modified residuei415Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VXK6.
PRIDEiQ9VXK6.

PTM databases

iPTMnetiQ9VXK6.

Expressioni

Gene expression databases

BgeeiFBgn0030719.
GenevisibleiQ9VXK6. DM.

Interactioni

Protein-protein interaction databases

BioGridi58910. 7 interactors.
DIPiDIP-21354N.
IntActiQ9VXK6. 1 interactor.
MINTiMINT-1328067.
STRINGi7227.FBpp0073947.

Structurei

3D structure databases

ProteinModelPortaliQ9VXK6.
SMRiQ9VXK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini254 – 415W2PROSITE-ProRule annotationAdd BLAST162

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi218 – 221Poly-Asp4

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Phylogenomic databases

eggNOGiKOG2767. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000016478.
InParanoidiQ9VXK6.
KOiK03262.
OMAiDDKFYRY.
OrthoDBiEOG091G0AKW.
PhylomeDBiQ9VXK6.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
PfamiView protein in Pfam
PF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
SMARTiView protein in SMART
SM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiView protein in PROSITE
PS51363. W2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VXK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVNVNRSV TDIFYRYKMP RLQAKVEGKG NGIKTVLVNM AEVARAIGRP
60 70 80 90 100
ATYPTKYFGC ELGAQTLFDH KNERFVVNGS HDVNKLQDLL DGFIRKFVLC
110 120 130 140 150
PECDNPETNL TVSAKNQTIS QSCKACGFHG LLKVNHKVNT FIVKNPPSLN
160 170 180 190 200
PAAQGSSLTE GKRSRKQKQK NDNADGSMTN NSLANNSGGE SDGGNGTNQA
210 220 230 240 250
SQTEAEISAA IPEKTAKDDD DEGWSVDVSK EAIRARLQDL TDGAKGMTIS
260 270 280 290 300
DDYDKTEKER IDIFYELVKD KRDKKQLDDV QTHKELVIEA ERLDIINKAP
310 320 330 340 350
LVLAELLFTE NIIKDVQKNR PLLLRFTLNN PKAQRYLIGG VEQTVELHKG
360 370 380 390 400
ILMSKVAGIF KLFYDLDILD EAVILEWAQK VSKRHVSKNI AAEIHEKAMP
410 420 430 440 450
FVLWLKNAEE ESSESEEEED DESEEDNYVS SAGQRGGQRV VQRGIPRAVA
460
GDEDDEDDVN IDDI
Length:464
Mass (Da):51,700
Last modified:May 1, 2000 - v1
Checksum:iB236088EA54FD880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48553.1.
AE014298 Genomic DNA. Translation: AAS65378.1.
AE014298 Genomic DNA. Translation: AAS65379.1.
AE014298 Genomic DNA. Translation: AAS65380.1.
AE014298 Genomic DNA. Translation: AAS65381.1.
AE014298 Genomic DNA. Translation: AAS65382.1.
AY060843 mRNA. Translation: AAL28391.1.
RefSeqiNP_573098.1. NM_132870.4.
NP_727922.1. NM_167477.2.
NP_996477.1. NM_206754.2.
NP_996478.1. NM_206755.2.
NP_996479.1. NM_206756.2.
NP_996480.1. NM_206757.2.
NP_996481.1. NM_206758.2.
UniGeneiDm.173.

Genome annotation databases

EnsemblMetazoaiFBtr0074144; FBpp0073947; FBgn0030719.
FBtr0074145; FBpp0073948; FBgn0030719.
FBtr0074146; FBpp0089136; FBgn0030719.
FBtr0074147; FBpp0089137; FBgn0030719.
FBtr0074148; FBpp0089138; FBgn0030719.
FBtr0074149; FBpp0089139; FBgn0030719.
FBtr0074150; FBpp0089140; FBgn0030719.
GeneIDi32566.
KEGGidme:Dmel_CG9177.
UCSCiCG9177-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiIF5_DROME
AccessioniPrimary (citable) accession number: Q9VXK6
Secondary accession number(s): A4V4K0, Q0KHS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families