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Protein

Eukaryotic translation initiation factor 5

Gene

eIF5

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 358GTPSequence analysis

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: FlyBase

GO - Biological processi

  • neurogenesis Source: FlyBase
  • translational initiation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:eIF5
ORF Names:CG9177
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030719. eIF5.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Eukaryotic translation initiation factor 5PRO_0000212520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine1 Publication
Modified residuei412 – 4121Phosphoserine1 Publication
Modified residuei413 – 4131Phosphoserine1 Publication
Modified residuei415 – 4151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VXK6.
PRIDEiQ9VXK6.

PTM databases

iPTMnetiQ9VXK6.

Expressioni

Gene expression databases

BgeeiFBgn0030719.
GenevisibleiQ9VXK6. DM.

Interactioni

Protein-protein interaction databases

BioGridi58910. 4 interactions.
DIPiDIP-21354N.
IntActiQ9VXK6. 1 interaction.
MINTiMINT-1328067.
STRINGi7227.FBpp0073947.

Structurei

3D structure databases

ProteinModelPortaliQ9VXK6.
SMRiQ9VXK6. Positions 4-147, 254-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini254 – 415162W2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi218 – 2214Poly-Asp

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2767. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000016478.
InParanoidiQ9VXK6.
KOiK03262.
OMAiGIFKLFY.
OrthoDBiEOG091G0AKW.
PhylomeDBiQ9VXK6.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VXK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVNVNRSV TDIFYRYKMP RLQAKVEGKG NGIKTVLVNM AEVARAIGRP
60 70 80 90 100
ATYPTKYFGC ELGAQTLFDH KNERFVVNGS HDVNKLQDLL DGFIRKFVLC
110 120 130 140 150
PECDNPETNL TVSAKNQTIS QSCKACGFHG LLKVNHKVNT FIVKNPPSLN
160 170 180 190 200
PAAQGSSLTE GKRSRKQKQK NDNADGSMTN NSLANNSGGE SDGGNGTNQA
210 220 230 240 250
SQTEAEISAA IPEKTAKDDD DEGWSVDVSK EAIRARLQDL TDGAKGMTIS
260 270 280 290 300
DDYDKTEKER IDIFYELVKD KRDKKQLDDV QTHKELVIEA ERLDIINKAP
310 320 330 340 350
LVLAELLFTE NIIKDVQKNR PLLLRFTLNN PKAQRYLIGG VEQTVELHKG
360 370 380 390 400
ILMSKVAGIF KLFYDLDILD EAVILEWAQK VSKRHVSKNI AAEIHEKAMP
410 420 430 440 450
FVLWLKNAEE ESSESEEEED DESEEDNYVS SAGQRGGQRV VQRGIPRAVA
460
GDEDDEDDVN IDDI
Length:464
Mass (Da):51,700
Last modified:May 1, 2000 - v1
Checksum:iB236088EA54FD880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48553.1.
AE014298 Genomic DNA. Translation: AAS65378.1.
AE014298 Genomic DNA. Translation: AAS65379.1.
AE014298 Genomic DNA. Translation: AAS65380.1.
AE014298 Genomic DNA. Translation: AAS65381.1.
AE014298 Genomic DNA. Translation: AAS65382.1.
AY060843 mRNA. Translation: AAL28391.1.
RefSeqiNP_573098.1. NM_132870.4.
NP_727922.1. NM_167477.2.
NP_996477.1. NM_206754.2.
NP_996478.1. NM_206755.2.
NP_996479.1. NM_206756.2.
NP_996480.1. NM_206757.2.
NP_996481.1. NM_206758.2.
UniGeneiDm.173.

Genome annotation databases

EnsemblMetazoaiFBtr0074144; FBpp0073947; FBgn0030719.
FBtr0074145; FBpp0073948; FBgn0030719.
FBtr0074146; FBpp0089136; FBgn0030719.
FBtr0074147; FBpp0089137; FBgn0030719.
FBtr0074148; FBpp0089138; FBgn0030719.
FBtr0074149; FBpp0089139; FBgn0030719.
FBtr0074150; FBpp0089140; FBgn0030719.
GeneIDi32566.
KEGGidme:Dmel_CG9177.
UCSCiCG9177-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF48553.1.
AE014298 Genomic DNA. Translation: AAS65378.1.
AE014298 Genomic DNA. Translation: AAS65379.1.
AE014298 Genomic DNA. Translation: AAS65380.1.
AE014298 Genomic DNA. Translation: AAS65381.1.
AE014298 Genomic DNA. Translation: AAS65382.1.
AY060843 mRNA. Translation: AAL28391.1.
RefSeqiNP_573098.1. NM_132870.4.
NP_727922.1. NM_167477.2.
NP_996477.1. NM_206754.2.
NP_996478.1. NM_206755.2.
NP_996479.1. NM_206756.2.
NP_996480.1. NM_206757.2.
NP_996481.1. NM_206758.2.
UniGeneiDm.173.

3D structure databases

ProteinModelPortaliQ9VXK6.
SMRiQ9VXK6. Positions 4-147, 254-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58910. 4 interactions.
DIPiDIP-21354N.
IntActiQ9VXK6. 1 interaction.
MINTiMINT-1328067.
STRINGi7227.FBpp0073947.

PTM databases

iPTMnetiQ9VXK6.

Proteomic databases

PaxDbiQ9VXK6.
PRIDEiQ9VXK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074144; FBpp0073947; FBgn0030719.
FBtr0074145; FBpp0073948; FBgn0030719.
FBtr0074146; FBpp0089136; FBgn0030719.
FBtr0074147; FBpp0089137; FBgn0030719.
FBtr0074148; FBpp0089138; FBgn0030719.
FBtr0074149; FBpp0089139; FBgn0030719.
FBtr0074150; FBpp0089140; FBgn0030719.
GeneIDi32566.
KEGGidme:Dmel_CG9177.
UCSCiCG9177-RA. d. melanogaster.

Organism-specific databases

CTDi1983.
FlyBaseiFBgn0030719. eIF5.

Phylogenomic databases

eggNOGiKOG2767. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000016478.
InParanoidiQ9VXK6.
KOiK03262.
OMAiGIFKLFY.
OrthoDBiEOG091G0AKW.
PhylomeDBiQ9VXK6.

Enzyme and pathway databases

ReactomeiR-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

ChiTaRSieIF5. fly.
GenomeRNAii32566.
PROiQ9VXK6.

Gene expression databases

BgeeiFBgn0030719.
GenevisibleiQ9VXK6. DM.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF5_DROME
AccessioniPrimary (citable) accession number: Q9VXK6
Secondary accession number(s): A4V4K0, Q0KHS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.