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Q9VXF1

- PP2B3_DROME

UniProt

Q9VXF1 - PP2B3_DROME

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit 3

Gene

CanA-14F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 4 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi164 – 1641IronBy similarity
    Metal bindingi166 – 1661IronBy similarity
    Metal bindingi192 – 1921IronBy similarity
    Metal bindingi192 – 1921ZincBy similarity
    Metal bindingi224 – 2241ZincBy similarity
    Active sitei225 – 2251Proton donorBy similarity
    Metal bindingi273 – 2731ZincBy similarity
    Metal bindingi355 – 3551ZincBy similarity

    GO - Molecular functioni

    1. calmodulin-dependent protein phosphatase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. protein serine/threonine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. positive regulation of NFAT protein import into nucleus Source: FlyBase
    2. protein dephosphorylation Source: FlyBase
    3. sleep Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calcium, Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_184321. Ca2+ pathway.
    REACT_203584. FCERI mediated Ca+2 mobilization.
    REACT_213958. DARPP-32 events.
    SignaLinkiQ9VXF1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit 3 (EC:3.1.3.16)
    Alternative name(s):
    Calmodulin-dependent calcineurin A3 subunit
    Gene namesi
    Name:CanA-14FImported
    Synonyms:CnnA14D1 Publication
    ORF Names:CG9819
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0030758. CanA-14F.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Serine/threonine-protein phosphatase 2B catalytic subunit 3PRO_0000308356Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei61 – 611Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9VXF1.

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically in embryos, larvae and adults.1 Publication

    Gene expression databases

    BgeeiQ9VXF1.

    Interactioni

    Subunit structurei

    Interacts with sra in a complex that contains Pp2B-14D.2 Publications

    Protein-protein interaction databases

    STRINGi7227.FBpp0074067.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VXF1.
    SMRiQ9VXF1. Positions 94-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    InParanoidiQ9VXF1.
    KOiK04348.
    OMAiXATAAAR.
    OrthoDBiEOG7BZVSC.
    PhylomeDBiQ9VXF1.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9VXF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSPAAQSNS SSSQSQSAAQ QQQQQNQKAN VNNTHDNKNA AATTGTAAGS    50
    GSGGAAGSAG TQQQGQGGTG TSSGPSSPTK RSTISTKERV IDSVAFPPSR 100
    KLTCADVFDA RTGKPQHDVL KQHFILEGRI EESAALRIIQ EGATLLRTEK 150
    TMIDIEAPVT VCGDIHGQFY DLMKLFEIGG SPATTKYLFL GDYVDRGYFS 200
    IECVLYLWSL KITYPQTLFL LRGNHECRHL TEYFTFKQEC KIKYSERVYD 250
    ACMDAFDCLP LAALMNQQFL CVHGGLSPEI HELEDIRRLD RFKEPPAFGP 300
    MCDLLWSDPL EDFGNEKNSD FYTHNSVRGC SYFYSYAACC DFLQNNNLLS 350
    IIRAHEAQDA GYRMYRKSQT TGFPSLITIF SAPNYLDVYN NKAAVLKYEN 400
    NVMNIRQFNC SPHPYWLPNF MDVFTWSLPF VGEKVTEMLV NVLNICSDDE 450
    LMTEESEEPL SDDEAALRKE VIRNKIRAIG KMARVFSVLR EESESVLQLK 500
    GLTPTGALPL GALSGGKQSL KNAMQGFSPN HKITSFAEAK GLDAVNERMP 550
    PRRDATPSPA EEGQKSLSAA AAAAANANAN SING 584
    Length:584
    Mass (Da):64,285
    Last modified:December 7, 2004 - v4
    Checksum:iB9428CE90283984C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti555 – 58430ATPSP…NSING → QPPTPSEDPNQHSQQGGKNG AGHG in AAC47079. (PubMed:8849894)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30493 mRNA. Translation: AAC47079.1.
    AE014298 Genomic DNA. Translation: AAF48623.4.
    AE014298 Genomic DNA. Translation: AAN09410.2.
    BT004864 mRNA. Translation: AAO45220.1.
    PIRiS70554.
    RefSeqiNP_001245717.1. NM_001258788.2.
    NP_001259622.1. NM_001272693.1.
    NP_727985.2. NM_167523.3.
    NP_727986.2. NM_167524.3.
    UniGeneiDm.33523.

    Genome annotation databases

    EnsemblMetazoaiFBtr0074292; FBpp0074067; FBgn0030758.
    FBtr0074293; FBpp0074068; FBgn0030758.
    FBtr0305198; FBpp0293728; FBgn0030758.
    FBtr0332870; FBpp0305092; FBgn0030758.
    GeneIDi8674098.
    KEGGidme:Dmel_CG9819.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30493 mRNA. Translation: AAC47079.1 .
    AE014298 Genomic DNA. Translation: AAF48623.4 .
    AE014298 Genomic DNA. Translation: AAN09410.2 .
    BT004864 mRNA. Translation: AAO45220.1 .
    PIRi S70554.
    RefSeqi NP_001245717.1. NM_001258788.2.
    NP_001259622.1. NM_001272693.1.
    NP_727985.2. NM_167523.3.
    NP_727986.2. NM_167524.3.
    UniGenei Dm.33523.

    3D structure databases

    ProteinModelPortali Q9VXF1.
    SMRi Q9VXF1. Positions 94-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0074067.

    Proteomic databases

    PaxDbi Q9VXF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0074292 ; FBpp0074067 ; FBgn0030758 .
    FBtr0074293 ; FBpp0074068 ; FBgn0030758 .
    FBtr0305198 ; FBpp0293728 ; FBgn0030758 .
    FBtr0332870 ; FBpp0305092 ; FBgn0030758 .
    GeneIDi 8674098.
    KEGGi dme:Dmel_CG9819.

    Organism-specific databases

    CTDi 8674098.
    FlyBasei FBgn0030758. CanA-14F.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    InParanoidi Q9VXF1.
    KOi K04348.
    OMAi XATAAAR.
    OrthoDBi EOG7BZVSC.
    PhylomeDBi Q9VXF1.

    Enzyme and pathway databases

    Reactomei REACT_184321. Ca2+ pathway.
    REACT_203584. FCERI mediated Ca+2 mobilization.
    REACT_213958. DARPP-32 events.
    SignaLinki Q9VXF1.

    Miscellaneous databases

    GenomeRNAii 8674098.
    NextBioi 779525.

    Gene expression databases

    Bgeei Q9VXF1.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of neurally expressing genes in the para sodium channel gene cluster of Drosophila."
      Hong C.-S., Ganetzky B.
      Genetics 142:879-892(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: Canton-SImported.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BerkeleyImported.
      Tissue: Embryo.
    5. "The calcineurin regulator sra plays an essential role in female meiosis in Drosophila."
      Takeo S., Tsuda M., Akahori S., Matsuo T., Aigaki T.
      Curr. Biol. 16:1435-1440(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SRA.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.
    7. "Shaggy/glycogen synthase kinase 3beta and phosphorylation of Sarah/regulator of calcineurin are essential for completion of Drosophila female meiosis."
      Takeo S., Swanson S.K., Nandanan K., Nakai Y., Aigaki T., Washburn M.P., Florens L., Hawley R.S.
      Proc. Natl. Acad. Sci. U.S.A. 109:6382-6389(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRA.

    Entry informationi

    Entry nameiPP2B3_DROME
    AccessioniPrimary (citable) accession number: Q9VXF1
    Secondary accession number(s): Q27573, Q86NL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 106 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3