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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit 3

Gene

CanA-14F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi164IronBy similarity1
Metal bindingi166IronBy similarity1
Metal bindingi192IronBy similarity1
Metal bindingi192ZincBy similarity1
Metal bindingi224ZincBy similarity1
Active sitei225Proton donorBy similarity1
Metal bindingi273ZincBy similarity1
Metal bindingi355ZincBy similarity1

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB-KW
  • calmodulin-dependent protein phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  • protein dephosphorylation Source: FlyBase
  • sleep Source: FlyBase

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase, Protein phosphatase
LigandCalcium, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-2025928 Calcineurin activates NFAT
R-DME-2871809 FCERI mediated Ca+2 mobilization
R-DME-4086398 Ca2+ pathway
R-DME-5607763 CLEC7A (Dectin-1) induces NFAT activation
SignaLinkiQ9VXF1

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit 3 (EC:3.1.3.16)
Alternative name(s):
Calmodulin-dependent calcineurin A3 subunit
Gene namesi
Name:CanA-14FImported
Synonyms:CnnA14D1 Publication
ORF Names:CG9819
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0267912 CanA-14F

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003083561 – 584Serine/threonine-protein phosphatase 2B catalytic subunit 3Add BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VXF1
PRIDEiQ9VXF1

PTM databases

iPTMnetiQ9VXF1

Expressioni

Developmental stagei

Expressed both maternally and zygotically in embryos, larvae and adults.1 Publication

Gene expression databases

BgeeiFBgn0030758
ExpressionAtlasiQ9VXF1 differential
GenevisibleiQ9VXF1 DM

Interactioni

Subunit structurei

Interacts with sra in a complex that contains Pp2B-14D.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1073112, 4 interactors
IntActiQ9VXF1, 3 interactors
STRINGi7227.FBpp0293728

Structurei

3D structure databases

ProteinModelPortaliQ9VXF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Sequence analysis

Phylogenomic databases

eggNOGiKOG0375 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000063087
InParanoidiQ9VXF1
KOiK04348
OMAiLWSLKIW
OrthoDBiEOG091G094R
PhylomeDBiQ9VXF1

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VXF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPAAQSNS SSSQSQSAAQ QQQQQNQKAN VNNTHDNKNA AATTGTAAGS
60 70 80 90 100
GSGGAAGSAG TQQQGQGGTG TSSGPSSPTK RSTISTKERV IDSVAFPPSR
110 120 130 140 150
KLTCADVFDA RTGKPQHDVL KQHFILEGRI EESAALRIIQ EGATLLRTEK
160 170 180 190 200
TMIDIEAPVT VCGDIHGQFY DLMKLFEIGG SPATTKYLFL GDYVDRGYFS
210 220 230 240 250
IECVLYLWSL KITYPQTLFL LRGNHECRHL TEYFTFKQEC KIKYSERVYD
260 270 280 290 300
ACMDAFDCLP LAALMNQQFL CVHGGLSPEI HELEDIRRLD RFKEPPAFGP
310 320 330 340 350
MCDLLWSDPL EDFGNEKNSD FYTHNSVRGC SYFYSYAACC DFLQNNNLLS
360 370 380 390 400
IIRAHEAQDA GYRMYRKSQT TGFPSLITIF SAPNYLDVYN NKAAVLKYEN
410 420 430 440 450
NVMNIRQFNC SPHPYWLPNF MDVFTWSLPF VGEKVTEMLV NVLNICSDDE
460 470 480 490 500
LMTEESEEPL SDDEAALRKE VIRNKIRAIG KMARVFSVLR EESESVLQLK
510 520 530 540 550
GLTPTGALPL GALSGGKQSL KNAMQGFSPN HKITSFAEAK GLDAVNERMP
560 570 580
PRRDATPSPA EEGQKSLSAA AAAAANANAN SING
Length:584
Mass (Da):64,285
Last modified:December 7, 2004 - v4
Checksum:iB9428CE90283984C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti555 – 584ATPSP…NSING → QPPTPSEDPNQHSQQGGKNG AGHG in AAC47079 (PubMed:8849894).CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30493 mRNA Translation: AAC47079.1
AE014298 Genomic DNA Translation: AAF48623.4
AE014298 Genomic DNA Translation: AAN09410.2
BT004864 mRNA Translation: AAO45220.1
PIRiS70554
RefSeqiNP_001245717.1, NM_001258788.2
NP_001259622.1, NM_001272693.2
NP_727985.2, NM_167523.3
NP_727986.2, NM_167524.3
UniGeneiDm.33523

Genome annotation databases

EnsemblMetazoaiFBtr0074292; FBpp0074067; FBgn0267912
FBtr0074293; FBpp0074068; FBgn0267912
FBtr0305198; FBpp0293728; FBgn0267912
FBtr0332870; FBpp0305092; FBgn0267912
GeneIDi8674098
KEGGidme:Dmel_CG9819

Similar proteinsi

Entry informationi

Entry nameiPP2B3_DROME
AccessioniPrimary (citable) accession number: Q9VXF1
Secondary accession number(s): Q27573, Q86NL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: December 7, 2004
Last modified: April 25, 2018
This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health