Reviewed, UniProtKB/Swiss-Prot Q9VX91 (UBR1_DROME)

Names and origin

Protein names

Recommended name:
    E3 ubiquitin-protein ligase UBR1
    EC=6.3.2.-
Alternative name(s):
    N-recognin
    Ubiquitin-protein ligase E3-alpha

Gene names

Name:	UBR1
ORF Names:	CG9086

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1824 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation By similarity.
Pathway	Protein modification; protein ubiquitination.
Domain	The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
Sequence similarities	Belongs to the UBR1 family. Contains 1 RING-type zinc finger. Contains 1 UBR-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1824	1824	E3 ubiquitin-protein ligase UBR1		PRO_0000056139
Regions
	Zinc finger	107 – 178	72	UBR-type
	Zinc finger	1126 – 1220	95	RING-type; atypical
Experimental info
	Sequence conflict	1661	1	E → V in AAM11168. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9VX91-1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 79992C11175E82A0	FASTA	1,824	208,361
	10 20 30 40 50 60 MDRYDMEDVV VAPPAECSSP LKEWRLKRQA GTLDRSDIIE FLKRESPKYF DYQTSATVKD 70 80 90 100 110 120 TNVITLKCMF KESLAKEEII DVVVEFMLGD NPSSALEKLR LEGNTATVCG KVFKNGEPTY 130 140 150 160 170 180 SCRECGVDPT CVLCVNCFKR SAHRFHKYKM STSGGGGCCD CGDDEAWKKD QYCELHLANR 190 200 210 220 230 240 KNPLESKILT DAVLERVEIC FGAILAFCVS YLEIEPNASL QCLDGNVEGG QVDGAQYCTV 250 260 270 280 290 300 LYNDESHTFD QVIQTLTKIA KCRAKDAMEI VAAIDREGRA VVKCDTFEEC NKLKVSIENQ 310 320 330 340 350 360 MILPTSLVST ARNNQSLRTS VLHIGAVACQ QFALQLLGWF QEFLVRHYLF RKTFSELVQR 370 380 390 400 410 420 KQETFCIRHI LEYDVKLWKT ARTCWHRLLI SGMLMEYDNK MILAQEFSRR YATIVEDFIS 430 440 450 460 470 480 DDHDHAFSIV SLSVQLFTVP SIAHHLIAHE GIFDKLLHTF YHVAIEKFIR NKTLHFSKNI 490 500 510 520 530 540 ASLTFFKRAN YILYDLRYLL SLKPDVLSND LRNGFLEGCR ALMRVLNVMQ GMESMTRQTG 550 560 570 580 590 600 QHMDYEPEWE CAFNLHIKLA TTISQVIDWA SGDVKLLRKL YKMTMRALVS NSFIVGGEKV 610 620 630 640 650 660 MQPKKVADHV ANCLVYDISV QPVSIHLPLS RFFAGIYLHL GAHDLTYDGL QTETEALSIK 670 680 690 700 710 720 LTPREIIEPV LCTQAMIAQV GAGLWRRNGY TLLHQLYFYR NVRCRVEMLD RDIACLQIGA 730 740 750 760 770 780 SLMESNEFLI HVLNRFNTIP WLQENYWSLL SGNEMNDDII REASIFDEFL ELLIVIIGER 790 800 810 820 830 840 WMPGVSMVTE EDRLRKEIIQ LLCIKPYSHS ELSRALPDGN SGNSDNVFEE VINTVAVFKK 850 860 870 880 890 900 PVGADSKGVY ELKEHLLKEF NMYFYHYTKE DKSKAEELQR ERRKAKKQLV CCPPPMLPKL 910 920 930 940 950 960 TPAFTPMANI LQCPVFLNIC SLIMERALNA YSRSFTESHL QKVLHLLGYA IQEELSEHYP 970 980 990 1000 1010 1020 FLSFYERSQE YGILEKLEEL ARCPRLEAHY DFVLWTIERF KQLQAKQAPS DGRAGPSCSQ 1030 1040 1050 1060 1070 1080 QGTGGKLSLS AEEQAREERE NRARLAAERR AHIMAQMQKA QKSFISSNAE MFADTENETR 1090 1100 1110 1120 1130 1140 KESASTGPMD WEDIPPEEEQ GAVALESKVA CLGPDRKFYH GTDDTFKCIL CFENCAISRG 1150 1160 1170 1180 1190 1200 GRQLVSSAFV QTSRVIFTTP NLRNSQSALH ISCCGHVMHY SCWLEYFTNE EFKELRRPHR 1210 1220 1230 1240 1250 1260 NRAALAQAAN VEFQCPYCRT LSNAIIPVTE TLPAFSAPPS PNESYLPLDS FVEIMSTLAI 1270 1280 1290 1300 1310 1320 ELGNVKDHEL TTLPSVSNIL RLSGVVGGLA QFERSVQLIK NPPRLHADYI EGIEFLKKAL 1330 1340 1350 1360 1370 1380 LNTMKIQQSH LKDHPAIESI EMVPILWDSC SYTLQALEIY LYAVEKPLKA ELSMRHQSCA 1390 1400 1410 1420 1430 1440 RNLVRACSRS SALEWETDLP LLPPMRSQAE FSSRLLDTIF NQNDTSVLEW DCFRVLVPFQ 1450 1460 1470 1480 1490 1500 FGVLNLMVPE KGYKTIIPSG SMFDFYIMQT MFLAQLTKAV LCFDVEKEKA KRAEKAPNSE 1510 1520 1530 1540 1550 1560 LTQLDYIEQL PSRIRDNMID FYRRYNIPAR VLQKTKQKQL VEEESEENQG HGQTVVIPCE 1570 1580 1590 1600 1610 1620 SHHLALLLEY VQRQMSSFLR CSCLFYRFLT DVDFPDTFPT DQPDRFDLMC QYLGLDPMLG 1630 1640 1650 1660 1670 1680 VYFDMETVYA TMMHSFASHP HIDREVEQRC QPDARRSLQV EPCLRPLPRL KVLCDDFSDL 1690 1700 1710 1720 1730 1740 INSVSDIFCP NNEREEMKTP TMCLICGLIL CGQSYCCQPE LGKVSVGACT HHAHACGAEV 1750 1760 1770 1780 1790 1800 GIFLRIRDCQ VVYLGRGKGC FVPPPYLDEY GETDMGLRRG NPLRLSQAAY RKIYLQWLGH 1810 1820 GLHGEIARLN DNANVAAAAQ WHHM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF48687.2. AY094815 mRNA. Translation: AAM11168.1. BT044223 mRNA. Translation: ACH92288.1.
RefSeq	NP_573184.1.
UniGene	Dm.7014
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VX91. 1 interaction.
Genome annotation databases
Ensembl	FBgn0030809. Drosophila melanogaster. [Contig view]
GeneID	32687.
KEGG	dme:Dmel_CG9086.
NMPDR	fig|7227.3.peg.18284.
Organism-specific databases
FlyBase	FBgn0030809. CG9086.
Phylogenomic databases
HOGENOM	Q9VX91.
OMA	Q9VX91. WESEWET.
Gene expression databases
ArrayExpress	Q9VX91.
GermOnline	CG9086. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003769. ClpS_core. IPR003126. Znf_N-recognin. IPR013993. Znf_N-recognin_met. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF02617. ClpS. 1 hit. PF02207. zf-UBR. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00396. ZnF_UBR1. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. PS51157. ZF_UBR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	779844.

Entry information

Entry name

UBR1_DROME

Accession

Primary (citable) accession number: Q9VX91
Secondary accession number(s): B5RIU9, Q8SX71

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project