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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

CG8188

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating polyubiquitin chains initiated by the E2 enzyme vih/UbcH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit.PROSITE-ProRule annotation1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • exit from mitosis Source: UniProtKB
  • protein polyubiquitination Source: GO_Central
  • protein ubiquitination Source: FlyBase

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ9VX25
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme S
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene namesi
ORF Names:CG8188
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0030863 CG8188

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904401 – 209Ubiquitin-conjugating enzyme E2 SAdd BLAST209

Proteomic databases

PaxDbiQ9VX25
PRIDEiQ9VX25

Expressioni

Gene expression databases

BgeeiFBgn0030863
ExpressionAtlasiQ9VX25 baseline and differential
GenevisibleiQ9VX25 DM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi59074, 9 interactors
IntActiQ9VX25, 5 interactors
STRINGi7227.FBpp0099891

Structurei

3D structure databases

ProteinModelPortaliQ9VX25
SMRiQ9VX25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0423 Eukaryota
COG5078 LUCA
InParanoidiQ9VX25
KOiK10583
OMAiGHKGEIC
OrthoDBiEOG091G0USF
PhylomeDBiQ9VX25

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VX25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQYSNVEN LSPQTIRQVM RELQEMETTP PEGIKVLINE SDVTDIQALI
60 70 80 90 100
DGPAGTPYAA GIFRVKLTLN KDFPLTPPKA YFLTKIFHPN VAANGEICVN
110 120 130 140 150
TLKKDWKPDL GIKHILLTIK CLLIVPNPES ALNEEAGKML LERYDDYSQR
160 170 180 190 200
ARMMTEIHAQ PAKCGVGAVG DAKDDGGPST KKHAGLDKKL QDKKKEKLLK

EKKRMLKRL
Length:209
Mass (Da):23,337
Last modified:July 5, 2004 - v2
Checksum:i65A528912E50A477
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ151896 mRNA Translation: AAZ57341.1
DQ151897 mRNA Translation: AAZ57342.1
AE014298 Genomic DNA Translation: ABC67190.1
AE014298 Genomic DNA Translation: AAF48756.2
BT031269 mRNA Translation: ABY20510.1
RefSeqiNP_001033852.1, NM_001038763.2
NP_001285383.1, NM_001298454.1
NP_001285384.1, NM_001298455.1
NP_573237.2, NM_133009.3
UniGeneiDm.11521

Genome annotation databases

EnsemblMetazoaiFBtr0074476; FBpp0074250; FBgn0030863
FBtr0100463; FBpp0099891; FBgn0030863
FBtr0340633; FBpp0309497; FBgn0030863
FBtr0346186; FBpp0312005; FBgn0030863
GeneIDi32751
KEGGidme:Dmel_CG8188
UCSCiCG8188-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiUBE2S_DROME
AccessioniPrimary (citable) accession number: Q9VX25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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