Reviewed, UniProtKB/Swiss-Prot Q9VWY6 (TAF8_DROME)

Names and origin

Protein names

Recommended name:
    Transcription initiation factor TFIID subunit 8
Alternative name(s):
    Protein prodos

Gene names

Name:	Taf8
Synonyms:	pds, prod
ORF Names:	CG7128

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Ref.5
Subunit structure	Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (TAFs). Histone fold interacts with N-terminus of Taf10b. Ref.5
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TAF8 family. Contains 1 histone-fold domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Ref.5 Inferred from direct assay. Source: UniProtKB
Cellular component	transcription factor TFIID complex Ref.5 Inferred from direct assay. Source: UniProtKB
Molecular function	RNA polymerase II transcription factor activity Ref.5 Inferred from direct assay. Source: UniProtKB protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Taf10b	Q9XZT7	1	EBI-128436,EBI-127738

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Transcription initiation factor TFIID subunit 8		PRO_0000118886
Regions
	Domain	16 – 83	68	Histone-fold
	Compositional bias	100 – 138	39	Pro-rich
Amino acid modifications
	Modified residue	236	1	Phosphoserine Ref.6
	Modified residue	245	1	Phosphoserine Ref.6
	Modified residue	255	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	164	1	Q → H in CAA75667. Ref.1
	Sequence conflict	250	1	M → I in AAL49226. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VWY6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A3CD62A4BC2EDC7C	FASTA	328	36,649
	10 20 30 40 50 60 MEKVEAVTVS NVDPYRRILN KVVSQLLLDK GAGQASNHSL ETLTQMLQAL IWEIGNSAHN 70 80 90 100 110 120 YCELSGRTMP TVGDVSLALI NMGISISNLD PYMRKETHVP IPLPPQQTQQ RPLSLLQAGI 130 140 150 160 170 180 KAPHPHYVPS YFPPMPDPHA YIRTPTHKQP VTEYEAIREK AACQKRDIEK ALTKFLCKTT 190 200 210 220 230 240 ETNNLFPTED NMFPLIACKP AFPPYAAALN PTDQVFDFEE LEYHYLVANR TEDEPSKDDG 250 260 270 280 290 300 EEGDSENEEM DGDKSKEEKP ELDIKPNSNT NKAILENPNI DNPYLRAATL PKRSKNCPTP 310 320 GTMPSRSLAT TAPTIRTPST LEITKTNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Ortuno-Sahagun D. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Prodos is a conserved transcriptional regulator that interacts with dTAF(II)16 in Drosophila melanogaster." Hernandez-Hernandez A., Ferrus A. Mol. Cell. Biol. 21:614-623(2001) [PubMed: 11134347] [Abstract] Cited for: FUNCTION, INTERACTION WITH TAF10B.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236; SER-245 AND SER-255, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y15513 Genomic DNA. Translation: CAA75667.1. AE014298 Genomic DNA. Translation: AAF48800.1. AY071604 mRNA. Translation: AAL49226.1.
RefSeq	NP_523397.1.
UniGene	Dm.2709
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VWY6. 1 interaction.
Proteomic databases
PRIDE	Q9VWY6.
Genome annotation databases
Ensembl	FBgn0022724. Drosophila melanogaster. [Contig view]
GeneID	32792.
KEGG	dme:Dmel_CG7128.
NMPDR	fig|7227.3.peg.18436.
Organism-specific databases
FlyBase	FBgn0022724. Taf8.
Phylogenomic databases
HOGENOM	Q9VWY6.
OMA	Q9VWY6. MFPLIAC.
Gene expression databases
ArrayExpress	Q9VWY6.
GermOnline	CG7128. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006565. BTP. IPR019473. TFIID_su8_C. [Graphical view]
Pfam	PF07524. Bromo_TP. 1 hit. PF10406. TAF8_C. 1 hit. [Graphical view]
SMART	SM00576. BTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	780406.

Entry information

Entry name

TAF8_DROME

Accession

Primary (citable) accession number: Q9VWY6
Secondary accession number(s): O18398, Q8SYE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project