Q9VWU1 (PSH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine protease persephone
EC=3.4.21.-

Gene names

Name:	psh
ORF Names:	CG6367

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the antifungal response cascade that activates spz. Nec regulates the cascade by inhibiting psh. Ref.1
Subcellular location	Secreted Ref.1.
Disruption phenotype	Flies lacking psh block induction of Drosomycin in the Toll pathway by fungi and resistance to fungal infection. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to fungus Inferred from mutant phenotype Ref.1. Source: UniProtKB innate immune response Inferred from direct assay PubMed 19590012. Source: FlyBase positive regulation of antifungal peptide biosynthetic process Inferred from mutant phenotype Ref.1. Source: FlyBase proteolysis Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of Toll signaling pathway Traceable author statement PubMed 14557290. Source: FlyBase
Cellular_component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	serine-type endopeptidase activity Non-traceable author statement PubMed 12568721. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 394	372	Serine protease persephone		PRO_0000271766
Regions
	Domain	144 – 389	246	Peptidase S1
Sites
	Active site	187	1	Charge relay system By similarity
	Active site	234	1	Charge relay system By similarity
	Active site	339	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Disulfide bond	172 ↔ 188		By similarity
	Disulfide bond	300 ↔ 324		By similarity
	Disulfide bond	335 ↔ 366		By similarity
Natural variations
	Natural variant	11	1	T → I in strain: G187. Ref.2
	Natural variant	14	1	L → H in strain: G136. Ref.2
	Natural variant	18	1	S → F in strain: G179. Ref.2
Experimental info
	Mutagenesis	187	1	H → Y in psh1 and psh4; immune response defective. Ref.1
	Mutagenesis	341	1	G → E in psh5; immune response defective. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9VWU1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 92D55383E459736E	FASTA	394	42,228
	10 20 30 40 50 60 MPLKWSLLLG TFVLISCSSV EAAVTVGRAC KVTDTMPGIC RTSSDCEPLI DGYIKSGVLT 70 80 90 100 110 120 LNDVPSCGLG AWGEIFCCPT KPCCDNSTIT SVSTSSTTST KAPMTSGRVD VPTFGSGDRP 130 140 150 160 170 180 AVAACKKIRE RKQQRSGNQL VIHIVGGYPV DPGVYPHMAA IGYITFGTDF RCGGSLIASR 190 200 210 220 230 240 FVLTAAHCVN TDANTPAFVR LGAVNIENPD HSYQDIVIRS VKIHPQYVGN KYNDIAILEL 250 260 270 280 290 300 ERDVVETDNI RPACLHTDAT DPPSNSKFFV AGWGVLNVTT RARSKILLRA GLELVPLDQC 310 320 330 340 350 360 NISYAEQPGS IRLLKQGVID SLLCAIDQKL IADACKGDSG GPLIHELNVE DGMYTIMGVI 370 380 390 SSGFGCATVT PGLYTRVSSY LDFIEGIVWP DNRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of Drosophila Toll during fungal infection by a blood serine protease." Ligoxygakis P., Pelte N., Hoffmann J.A., Reichhart J.-M. Science 297:114-116(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF HIS-187 AND GLY-341, DISRUPTION PHENOTYPE.
[2]	"A screen for immunity genes evolving under positive selection in Drosophila." Jiggins F.M., Kim K.W. J. Evol. Biol. 20:965-970(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-11; HIS-14 AND PHE-18. Strain: G124, G136, G145, G173, G179, G185, G186, G187, G191 and G193.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM412840 Genomic DNA. Translation: CAL85463.1. AM412841 Genomic DNA. Translation: CAL85464.1. AM412842 Genomic DNA. Translation: CAL85465.1. AM412843 Genomic DNA. Translation: CAL85466.1. AM412844 Genomic DNA. Translation: CAL85467.1. AM412845 Genomic DNA. Translation: CAL85468.1. AM412846 Genomic DNA. Translation: CAL85469.1. AM412847 Genomic DNA. Translation: CAL85470.1. AM412848 Genomic DNA. Translation: CAL85471.1. AM412849 Genomic DNA. Translation: CAL85472.1. AE014298 Genomic DNA. Translation: AAF48846.1. AY102662 mRNA. Translation: AAM27491.1.
RefSeq	NP_573297.1. NM_133069.1.
UniGene	Dm.18653.
3D structure databases
HSSP	HSSP built from PDB template 1RTF based on UniProtKB P00750.
ProteinModelPortal	Q9VWU1.
SMR	Q9VWU1. Positions 126-388.
ModBase	Search...
Protein family/group databases
MEROPS	S01.421.
Proteomic databases
PaxDb	Q9VWU1.
PRIDE	Q9VWU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074629; FBpp0074400; FBgn0030926.
GeneID	32832.
KEGG	dme:Dmel_CG6367.
UCSC	CG6367-RA. d. melanogaster.
Organism-specific databases
CTD	32832.
FlyBase	FBgn0030926. psh.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00700000104945.
InParanoid	Q9VWU1.
OMA	PGVYPHM.
OrthoDB	EOG4VMCWH.
PhylomeDB	Q9VWU1.
Gene expression databases
Bgee	Q9VWU1.
Family and domain databases
InterPro	IPR006604. Disulphide_knot_CLIP. IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00680. CLIP. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32832.
NextBio	780610.

Entry information

Entry name

PSH_DROME

Accession

Primary (citable) accession number: Q9VWU1
Secondary accession number(s): A4V9U0 , A4V9U1, A4V9U4, A4V9U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project