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Protein

Cytochrome P450 306a1

Gene

phm

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.1 Publication

Miscellaneous

Member of the Halloween gene group.

Caution

It is uncertain whether Met-1 or Met-14 is the initiator.Curated

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Pathwayi: ecdysteroid biosynthesis

This protein is involved in the pathway ecdysteroid biosynthesis, which is part of Steroid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway ecdysteroid biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi505Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • ecdysone biosynthetic process Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • oogenesis Source: FlyBase

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18105
ReactomeiR-DME-193048 Androgen biosynthesis
R-DME-193993 Mineralocorticoid biosynthesis
R-DME-194002 Glucocorticoid biosynthesis
R-DME-211976 Endogenous sterols
UniPathwayiUPA00765

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 306a1 (EC:1.14.-.-)
Short name:
CYPCCCVIA1
Alternative name(s):
Protein phantom
Short name:
Dmphm
Gene namesi
Name:phm
Synonyms:Cyp306a1
ORF Names:CG6578
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004959 phm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000523151 – 574Cytochrome P450 306a1Add BLAST574

Proteomic databases

PaxDbiQ9VWR5
PRIDEiQ9VWR5

Expressioni

Tissue specificityi

First seen at the early (syncytial) blastoderm stage 4. During cellularization of the blastoderm (stage 5), stripes of expression appear and remain through to stage 10. Expression becomes undetectable during germ band retraction (stages 11-14). By stage 15, some expression resumes in the primordium of the ring gland, so that by stage 17 strong expression is seen, but only in the ring gland. This specific localization continues throughout the larval instars (at protein level). Expressed in the prothoracic gland cells of the larval ring gland (RG). Levels decline just after the molt to the third instar then increase later during the wandering stage. Low levels of expression are seen in the larval brain and fat body. In the adult, majority of expression is restricted to the ovaries, with low levels in the head and carcass of both sexes.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0004959
ExpressionAtlasiQ9VWR5 differential
GenevisibleiQ9VWR5 DM

Interactioni

Protein-protein interaction databases

BioGridi59169, 3 interactors
DIPiDIP-21151N
IntActiQ9VWR5, 4 interactors
STRINGi7227.FBpp0074374

Structurei

3D structure databases

ProteinModelPortaliQ9VWR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
InParanoidiQ9VWR5
KOiK10720
OMAiRMCPGDE
OrthoDBiEOG091G0BT8
PhylomeDBiQ9VWR5

Family and domain databases

InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 2 hits
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VWR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADIVDIGH TGWMPSVQSL SILLVPGALV LVILYLCERQ CNDLMGAPPP
60 70 80 90 100
GPWGLPFLGY LPFLDARAPH KSLQKLAKRY GGIFELKMGR VPTVVLSDAA
110 120 130 140 150
LVRDFFRRDV MTGRAPLYLT HGIMGGFGII CAQEDIWRHA RRETIDWLKA
160 170 180 190 200
LGMTRRPGEL RARLERRIAR GVDECVRLFD TEAKKSCASE VNPLPALHHS
210 220 230 240 250
LGNIINDLVF GITYKRDDPD WLYLQRLQEE GVKLIGVSGV VNFLPWLRHL
260 270 280 290 300
PANVRNIRFL LEGKAKTHAI YDRIVEACGQ RLKEKQKVFK ELQEQKRLQR
310 320 330 340 350
QLEKEQLRQS KEADPSQEQS EADEDDEESD EEDTYEPECI LEHFLAVRDT
360 370 380 390 400
DSQLYCDDQL RHLLADLFGA GVDTSLATLR WFLLYLAREQ RCQRRLHELL
410 420 430 440 450
LPLGPSPTLE ELEPLAYLRA CISETMRIRS VVPLGIPHGC KENFVVGDYF
460 470 480 490 500
IKGGSMIVCS EWAIHMDPVA FPEPEEFRPE RFLTADGAYQ APPQFIPFSS
510 520 530 540 550
GYRMCPGEEM ARMILTLFTG RILRRFHLEL PSGTEVDMAG ESGITLTPTP
560 570
HMLRFTKLPA VEMRHAPDGA VVQD
Length:574
Mass (Da):65,177
Last modified:May 1, 2000 - v1
Checksum:i9A8A3E1747101700
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti399L → F in strain: CN13X. 1 Publication1
Natural varianti560A → T in strain: CN11X. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF484413 mRNA Translation: AAQ05971.1
AM411848 Genomic DNA Translation: CAL69929.1
AM411849 Genomic DNA Translation: CAL69931.1
AM411850 Genomic DNA Translation: CAL69933.1
AM411851 Genomic DNA Translation: CAL69935.1
AM411852 Genomic DNA Translation: CAL69937.1
AM411853 Genomic DNA Translation: CAL69939.1
AM411854 Genomic DNA Translation: CAL69941.1
AM411855 Genomic DNA Translation: CAL69943.1
AM411856 Genomic DNA Translation: CAL69945.1
AM411857 Genomic DNA Translation: CAL69947.1
AM411858 Genomic DNA Translation: CAL69949.1
AM411859 Genomic DNA Translation: CAL69951.1
AM411860 Genomic DNA Translation: CAL69953.1
AE014298 Genomic DNA Translation: AAF48873.1
AY070930 mRNA Translation: AAL48552.1
RefSeqiNP_001285414.1, NM_001298485.1
NP_573319.1, NM_133091.3
UniGeneiDm.5302

Genome annotation databases

EnsemblMetazoaiFBtr0074603; FBpp0074374; FBgn0004959
FBtr0342846; FBpp0309669; FBgn0004959
GeneIDi32857
KEGGidme:Dmel_CG6578
UCSCiCG6578-RA d. melanogaster

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCP306_DROME
AccessioniPrimary (citable) accession number: Q9VWR5
Secondary accession number(s): A5AC85, A5AC97, A5AC99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health