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Protein

Cytochrome P450 306a1

Gene

phm

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Pathwayi: ecdysteroid biosynthesis

This protein is involved in the pathway ecdysteroid biosynthesis, which is part of Steroid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway ecdysteroid biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi505 – 5051Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • border follicle cell migration Source: FlyBase
  • ecdysone biosynthetic process Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • insecticide metabolic process Source: FlyBase
  • oogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18105.
UniPathwayiUPA00765.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 306a1 (EC:1.14.-.-)
Short name:
CYPCCCVIA1
Alternative name(s):
Protein phantom
Short name:
Dmphm
Gene namesi
Name:phm
Synonyms:Cyp306a1
ORF Names:CG6578
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004959. phm.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: FlyBase
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Cytochrome P450 306a1PRO_0000052315Add
BLAST

Proteomic databases

PaxDbiQ9VWR5.
PRIDEiQ9VWR5.

Expressioni

Tissue specificityi

First seen at the early (syncytial) blastoderm stage 4. During cellularization of the blastoderm (stage 5), stripes of expression appear and remain through to stage 10. Expression becomes undetectable during germ band retraction (stages 11-14). By stage 15, some expression resumes in the primordium of the ring gland, so that by stage 17 strong expression is seen, but only in the ring gland. This specific localization continues throughout the larval instars (at protein level). Expressed in the prothoracic gland cells of the larval ring gland (RG). Levels decline just after the molt to the third instar then increase later during the wandering stage. Low levels of expression are seen in the larval brain and fat body. In the adult, majority of expression is restricted to the ovaries, with low levels in the head and carcass of both sexes.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiQ9VWR5.
ExpressionAtlasiQ9VWR5. differential.
GenevisibleiQ9VWR5. DM.

Interactioni

Protein-protein interaction databases

BioGridi59169. 3 interactions.
DIPiDIP-21151N.
IntActiQ9VWR5. 1 interaction.
MINTiMINT-317942.
STRINGi7227.FBpp0074374.

Structurei

3D structure databases

ProteinModelPortaliQ9VWR5.
SMRiQ9VWR5. Positions 46-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
InParanoidiQ9VWR5.
KOiK10720.
OMAiMSADIVD.
OrthoDBiEOG7SN8C8.
PhylomeDBiQ9VWR5.

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 2 hits.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VWR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADIVDIGH TGWMPSVQSL SILLVPGALV LVILYLCERQ CNDLMGAPPP
60 70 80 90 100
GPWGLPFLGY LPFLDARAPH KSLQKLAKRY GGIFELKMGR VPTVVLSDAA
110 120 130 140 150
LVRDFFRRDV MTGRAPLYLT HGIMGGFGII CAQEDIWRHA RRETIDWLKA
160 170 180 190 200
LGMTRRPGEL RARLERRIAR GVDECVRLFD TEAKKSCASE VNPLPALHHS
210 220 230 240 250
LGNIINDLVF GITYKRDDPD WLYLQRLQEE GVKLIGVSGV VNFLPWLRHL
260 270 280 290 300
PANVRNIRFL LEGKAKTHAI YDRIVEACGQ RLKEKQKVFK ELQEQKRLQR
310 320 330 340 350
QLEKEQLRQS KEADPSQEQS EADEDDEESD EEDTYEPECI LEHFLAVRDT
360 370 380 390 400
DSQLYCDDQL RHLLADLFGA GVDTSLATLR WFLLYLAREQ RCQRRLHELL
410 420 430 440 450
LPLGPSPTLE ELEPLAYLRA CISETMRIRS VVPLGIPHGC KENFVVGDYF
460 470 480 490 500
IKGGSMIVCS EWAIHMDPVA FPEPEEFRPE RFLTADGAYQ APPQFIPFSS
510 520 530 540 550
GYRMCPGEEM ARMILTLFTG RILRRFHLEL PSGTEVDMAG ESGITLTPTP
560 570
HMLRFTKLPA VEMRHAPDGA VVQD
Length:574
Mass (Da):65,177
Last modified:May 1, 2000 - v1
Checksum:i9A8A3E1747101700
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti399 – 3991L → F in strain: CN13X. 1 Publication
Natural varianti560 – 5601A → T in strain: CN11X. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF484413 mRNA. Translation: AAQ05971.1.
AM411848 Genomic DNA. Translation: CAL69929.1.
AM411849 Genomic DNA. Translation: CAL69931.1.
AM411850 Genomic DNA. Translation: CAL69933.1.
AM411851 Genomic DNA. Translation: CAL69935.1.
AM411852 Genomic DNA. Translation: CAL69937.1.
AM411853 Genomic DNA. Translation: CAL69939.1.
AM411854 Genomic DNA. Translation: CAL69941.1.
AM411855 Genomic DNA. Translation: CAL69943.1.
AM411856 Genomic DNA. Translation: CAL69945.1.
AM411857 Genomic DNA. Translation: CAL69947.1.
AM411858 Genomic DNA. Translation: CAL69949.1.
AM411859 Genomic DNA. Translation: CAL69951.1.
AM411860 Genomic DNA. Translation: CAL69953.1.
AE014298 Genomic DNA. Translation: AAF48873.1.
AY070930 mRNA. Translation: AAL48552.1.
RefSeqiNP_001285414.1. NM_001298485.1.
NP_573319.1. NM_133091.3.
UniGeneiDm.5302.

Genome annotation databases

EnsemblMetazoaiFBtr0074603; FBpp0074374; FBgn0004959.
FBtr0342846; FBpp0309669; FBgn0004959.
GeneIDi32857.
KEGGidme:Dmel_CG6578.
UCSCiCG6578-RA. d. melanogaster.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF484413 mRNA. Translation: AAQ05971.1.
AM411848 Genomic DNA. Translation: CAL69929.1.
AM411849 Genomic DNA. Translation: CAL69931.1.
AM411850 Genomic DNA. Translation: CAL69933.1.
AM411851 Genomic DNA. Translation: CAL69935.1.
AM411852 Genomic DNA. Translation: CAL69937.1.
AM411853 Genomic DNA. Translation: CAL69939.1.
AM411854 Genomic DNA. Translation: CAL69941.1.
AM411855 Genomic DNA. Translation: CAL69943.1.
AM411856 Genomic DNA. Translation: CAL69945.1.
AM411857 Genomic DNA. Translation: CAL69947.1.
AM411858 Genomic DNA. Translation: CAL69949.1.
AM411859 Genomic DNA. Translation: CAL69951.1.
AM411860 Genomic DNA. Translation: CAL69953.1.
AE014298 Genomic DNA. Translation: AAF48873.1.
AY070930 mRNA. Translation: AAL48552.1.
RefSeqiNP_001285414.1. NM_001298485.1.
NP_573319.1. NM_133091.3.
UniGeneiDm.5302.

3D structure databases

ProteinModelPortaliQ9VWR5.
SMRiQ9VWR5. Positions 46-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59169. 3 interactions.
DIPiDIP-21151N.
IntActiQ9VWR5. 1 interaction.
MINTiMINT-317942.
STRINGi7227.FBpp0074374.

Proteomic databases

PaxDbiQ9VWR5.
PRIDEiQ9VWR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074603; FBpp0074374; FBgn0004959.
FBtr0342846; FBpp0309669; FBgn0004959.
GeneIDi32857.
KEGGidme:Dmel_CG6578.
UCSCiCG6578-RA. d. melanogaster.

Organism-specific databases

CTDi32857.
FlyBaseiFBgn0004959. phm.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
InParanoidiQ9VWR5.
KOiK10720.
OMAiMSADIVD.
OrthoDBiEOG7SN8C8.
PhylomeDBiQ9VWR5.

Enzyme and pathway databases

UniPathwayiUPA00765.
BioCyciMetaCyc:MONOMER-18105.

Miscellaneous databases

GenomeRNAii32857.
PROiQ9VWR5.

Gene expression databases

BgeeiQ9VWR5.
ExpressionAtlasiQ9VWR5. differential.
GenevisibleiQ9VWR5. DM.

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 2 hits.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phantom encodes the 25-hydroxylase of Drosophila melanogaster and Bombyx mori: a P450 enzyme critical in ecdysone biosynthesis."
    Warren J.T., Petryk A., Marques G., Parvy J.P., Shinoda T., Itoyama K., Kobayashi J., Jarcho M., Li Y., O'Connor M.B., Dauphin-Villemant C., Gilbert L.I.
    Insect Biochem. Mol. Biol. 34:991-1010(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "Genome scans of variation and adaptive change: extended analysis of a candidate locus close to the phantom gene region in Drosophila melanogaster."
    Orengo D.J., Aguade M.
    Mol. Biol. Evol. 24:1122-1129(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS PHE-399 AND THR-560.
    Strain: CN10X, CN11X, CN13X, CN15X, CN16X, CN17X, CN19X, CN21X, CN22BX, CN3X and CN7X.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiCP306_DROME
AccessioniPrimary (citable) accession number: Q9VWR5
Secondary accession number(s): A5AC85, A5AC97, A5AC99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Member of the Halloween gene group.

Caution

It is uncertain whether Met-1 or Met-14 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.