Reviewed, UniProtKB/Swiss-Prot Q9VWH4 (IDH3A_DROME)

Names and origin

Protein names

Recommended name:
    Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:	l(1)G0156
ORF Names:	CG12233

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH. UniProtKB P56471
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity. UniProtKB P56471
Subcellular location	Mitochondrion By similarity. UniProtKB P56471
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Sequence caution	The sequence AAL90367.1 differs from that shown. Reason: Frameshift at position 205.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	lipid particle Inferred from direct assay. Source: FlyBase mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: Q9VWH4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: Q9VWH4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MAARFIQKILNQLGLIAARDAPAVTATPAVSQ → MAARFIQKI

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 377		Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014442
Sites
	Metal binding	249	1	Magnesium or manganese By similarity
	Metal binding	273	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Site	169	1	Critical for catalysis By similarity
	Site	216	1	Critical for catalysis By similarity
Natural variations
	Alternative sequence	1 – 32	32	MAARF…PAVSQ → MAARFIQKI in isoform A. Ref.1		VSP_050698

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FB3F30905B8939B3	FASTA	377	40,844
	10 20 30 40 50 60 MAARFIQKIL NQLGLIAARD APAVTATPAV SQVNATPAAS RSYSSGTKKV TLIPGDGIGP 70 80 90 100 110 120 EISAAVQKIF TAANVPIEWE AVDVTPVRGP DGKFGIPQAA IDSVNTNKIG LKGPLMTPVG 130 140 150 160 170 180 KGHRSLNLAL RKEFNLYANV RPCRSLEGYK TLYDDVDVVT IRENTEGEYS GIEHEIVDGV 190 200 210 220 230 240 VQSIKLITEE ASKRVAEYAF QYAKNNNRKK VTVVHKANIM RMSDGLFLRC VRDMAQKFPE 250 260 270 280 290 300 IQFEEKYLDT VCLNMVQNPG KYDVLVMPNL YGDILSDMCA GLVGGLGLTP SGNMGLNGAL 310 320 330 340 350 360 FESVHGTAPD IAGKDLANPT ALLLSAVMML RHMELNTYAD KIERAAFETI KEGKYLTGDL 370 GGRAKCSEFT NEICAKL
	Isoform A. Checksum: 64426D3BE3983BB4	FASTA	354	38,584
	10 20 30 40 50 60 MAARFIQKIV NATPAASRSY SSGTKKVTLI PGDGIGPEIS AAVQKIFTAA NVPIEWEAVD 70 80 90 100 110 120 VTPVRGPDGK FGIPQAAIDS VNTNKIGLKG PLMTPVGKGH RSLNLALRKE FNLYANVRPC 130 140 150 160 170 180 RSLEGYKTLY DDVDVVTIRE NTEGEYSGIE HEIVDGVVQS IKLITEEASK RVAEYAFQYA 190 200 210 220 230 240 KNNNRKKVTV VHKANIMRMS DGLFLRCVRD MAQKFPEIQF EEKYLDTVCL NMVQNPGKYD 250 260 270 280 290 300 VLVMPNLYGD ILSDMCAGLV GGLGLTPSGN MGLNGALFES VHGTAPDIAG KDLANPTALL 310 320 330 340 350 LSAVMMLRHM ELNTYADKIE RAAFETIKEG KYLTGDLGGR AKCSEFTNEI CAKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF48965.1. AE014298 Genomic DNA. Translation: AAN09496.1. AY089629 mRNA. Translation: AAL90367.1. Frameshift.
RefSeq	NP_573388.1. NP_728257.1.
UniGene	Dm.222
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VWH4. 4 interactions.
Proteomic databases
PRIDE	Q9VWH4.
Genome annotation databases
Ensembl	FBgn0027291. Drosophila melanogaster. [Contig view]
GeneID	32940.
KEGG	dme:Dmel_CG12233.
NMPDR	fig|7227.3.peg.18632.
Organism-specific databases
FlyBase	FBgn0027291. l(1)G0156.
Phylogenomic databases
HOGENOM	Q9VWH4.
OMA	Q9VWH4. SANIGHQ.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-002607-MON.
BRENDA	1.1.1.41. 48.
Gene expression databases
ArrayExpress	Q9VWH4.
GermOnline	CG12233. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	781145.

Entry information

Entry name

IDH3A_DROME

Accession

Primary (citable) accession number: Q9VWH4
Secondary accession number(s): Q8IQW9, Q8SXH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project