Q9VWH4 (IDH3A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
EC=1.1.1.41

Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha

Gene names

Name:	l(1)G0156
ORF Names:	CG12233

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH. UniProtKB P56471
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity. UniProtKB P56471
Subcellular location	Mitochondrion By similarity UniProtKB P56471.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Sequence caution	The sequence AAL90367.1 differs from that shown. Reason: Frameshift at position 205.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	lipid particle Inferred from direct assay. Source: FlyBase microtubule associated complex Inferred from direct assay. Source: FlyBase mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: Q9VWH4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: Q9VWH4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MAARFIQKILNQLGLIAARDAPAVTATPAVSQ → MAARFIQKI

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 377		Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014442
Sites
	Metal binding	249	1	Magnesium or manganese By similarity
	Metal binding	273	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Site	169	1	Critical for catalysis By similarity
	Site	216	1	Critical for catalysis By similarity
Natural variations
	Alternative sequence	1 – 32	32	MAARF…PAVSQ → MAARFIQKI in isoform A. Ref.1		VSP_050698

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FB3F30905B8939B3	FASTA	377	40,844
	10 20 30 40 50 60 MAARFIQKIL NQLGLIAARD APAVTATPAV SQVNATPAAS RSYSSGTKKV TLIPGDGIGP 70 80 90 100 110 120 EISAAVQKIF TAANVPIEWE AVDVTPVRGP DGKFGIPQAA IDSVNTNKIG LKGPLMTPVG 130 140 150 160 170 180 KGHRSLNLAL RKEFNLYANV RPCRSLEGYK TLYDDVDVVT IRENTEGEYS GIEHEIVDGV 190 200 210 220 230 240 VQSIKLITEE ASKRVAEYAF QYAKNNNRKK VTVVHKANIM RMSDGLFLRC VRDMAQKFPE 250 260 270 280 290 300 IQFEEKYLDT VCLNMVQNPG KYDVLVMPNL YGDILSDMCA GLVGGLGLTP SGNMGLNGAL 310 320 330 340 350 360 FESVHGTAPD IAGKDLANPT ALLLSAVMML RHMELNTYAD KIERAAFETI KEGKYLTGDL 370 GGRAKCSEFT NEICAKL
	Isoform A [UniParc]. Checksum: 64426D3BE3983BB4	FASTA	354	38,584
	10 20 30 40 50 60 MAARFIQKIV NATPAASRSY SSGTKKVTLI PGDGIGPEIS AAVQKIFTAA NVPIEWEAVD 70 80 90 100 110 120 VTPVRGPDGK FGIPQAAIDS VNTNKIGLKG PLMTPVGKGH RSLNLALRKE FNLYANVRPC 130 140 150 160 170 180 RSLEGYKTLY DDVDVVTIRE NTEGEYSGIE HEIVDGVVQS IKLITEEASK RVAEYAFQYA 190 200 210 220 230 240 KNNNRKKVTV VHKANIMRMS DGLFLRCVRD MAQKFPEIQF EEKYLDTVCL NMVQNPGKYD 250 260 270 280 290 300 VLVMPNLYGD ILSDMCAGLV GGLGLTPSGN MGLNGALFES VHGTAPDIAG KDLANPTALL 310 320 330 340 350 LSAVMMLRHM ELNTYADKIE RAAFETIKEG KYLTGDLGGR AKCSEFTNEI CAKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014298 Genomic DNA. Translation: AAF48965.1. AE014298 Genomic DNA. Translation: AAN09496.1. AY089629 mRNA. Translation: AAL90367.1. Frameshift.
RefSeq	NP_573388.1. NM_133160.1. NP_728257.1. NM_167657.1.
UniGene	Dm.222.
3D structure databases
ProteinModelPortal	Q9VWH4.
SMR	Q9VWH4. Positions 39-377.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-898629.
STRING	Q9VWH4.
Proteomic databases
PRIDE	Q9VWH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	32940.
KEGG	dme:Dmel_CG12233.
NMPDR	fig|7227.3.peg.18632.
Organism-specific databases
FlyBase	FBgn0027291. l(1)G0156.
Phylogenomic databases
eggNOG	inNOG04170.
GeneTree	EMGT00050000005801.
InParanoid	Q9VWH4.
OMA	QPSIGRY.
OrthoDB	EOG4X0K7X.
PhylomeDB	Q9VWH4.
Gene expression databases
ArrayExpress	Q9VWH4.
Bgee	Q9VWH4.
GermOnline	CG12233. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KO	K00030.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF5. PTHR11835:SF5. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. Mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	781145.

Entry information

Entry name

IDH3A_DROME

Accession

Primary (citable) accession number: Q9VWH4
Secondary accession number(s): Q8IQW9, Q8SXH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project