Reviewed, UniProtKB/Swiss-Prot Q9VW60 (ADCY2_DROME)

Names and origin

Protein names

Recommended name:
    Adenylate cyclase type 2
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase 2
    Adenylyl cyclase 76E

Gene names

Name:	Ac76E
ORF Names:	CG7978

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1307 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a membrane-bound, calmodulin-insensitive adenylyl cyclase By similarity. UniProtKB P26769
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate. UniProtKB P26769
Cofactor	Binds 2 magnesium ions per subunit By similarity. UniProtKB P30803
Enzyme regulation	Insensitive to calcium/calmodulin. Stimulated by the G protein beta and gamma subunit complex By similarity. UniProtKB P26769
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Non-traceable author statement. Source: FlyBase magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1307	1307	Adenylate cyclase type 2		PRO_0000195713
Regions
	Transmembrane	85 – 105	21	Potential
	Transmembrane	113 – 133	21	Potential
	Transmembrane	137 – 157	21	Potential
	Transmembrane	178 – 198	21	Potential
	Transmembrane	211 – 231	21	Potential
	Topological domain	232 – 828	597	Cytoplasmic Potential
	Transmembrane	829 – 849	21	Potential
	Transmembrane	851 – 871	21	Potential
	Transmembrane	900 – 920	21	Potential
	Transmembrane	963 – 983	21	Potential
	Transmembrane	987 – 1007	21	Potential
	Transmembrane	1024 – 1044	21	Potential
	Topological domain	1045 – 1307	263	Cytoplasmic Potential
	Domain	317 – 444	128	Guanylate cyclase 1
	Domain	1110 – 1255	146	Guanylate cyclase 2
Sites
	Metal binding	322	1	Magnesium 1 By similarity UniProtKB P30803
	Metal binding	322	1	Magnesium 2 By similarity UniProtKB P30803
	Metal binding	323	1	Magnesium 2; via carbonyl oxygen By similarity UniProtKB P30803
	Metal binding	366	1	Magnesium 1 By similarity UniProtKB P30803
	Metal binding	366	1	Magnesium 2 By similarity UniProtKB P30803
Amino acid modifications
	Glycosylation	934	1	N-linked (GlcNAc...) Potential
	Glycosylation	941	1	N-linked (GlcNAc...) Potential
	Glycosylation	948	1	N-linked (GlcNAc...) Potential
	Glycosylation	1019	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	538	1	V → G in AAC62509. Ref.1
	Sequence conflict	679	1	A → VSS in AAC62509. Ref.1
	Sequence conflict	1037	1	I → T in AAC62509. Ref.1
	Sequence conflict	1183	1	T → TSRSFA in AAM50201. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VW60-1 [UniParc]. Last modified October 1, 2002. Version 3. Checksum: D7EE45CF93F80161	FASTA	1,307	142,817
	10 20 30 40 50 60 MVNHNAETAK TGNGTNATAN LIVKADGNAT QPKAMTSSAA RMNDALSASL ADLSEQENGT 70 80 90 100 110 120 TAEDIHLNDL YTRYRQRLRK SLFRSGLLTS LLACVVSIII GIVYGQHLVQ TMLLVLAALI 130 140 150 160 170 180 SGSILTALQF PAVLSSPAAA LAFAIVTTFS LGTIAAITGD ELAPLPMYAL FLCIHSMLPI 190 200 210 220 230 240 SWPVSVVLAL FMTAIHIVYR IGTSPDYAPN LPMLFGEIVM LASASVSGLY YRIMSDAAHN 250 260 270 280 290 300 RTVDGTRTGI EQRVKLECER EQQEQLLLSV IPAYIAAEVK RSIMLKMADA CQRAGGQAST 310 320 330 340 350 360 SATRFHELHV QRHTNVTILF ADIVNFTPLS SSLTASDLVK TLNDLFGRFD QIAQENQCLR 370 380 390 400 410 420 IKILGDCYYC VSGLPISRPQ HATNCVNMGL QMIDAIRHVR EATGINVDMR IGIHTGNVLC 430 440 450 460 470 480 GVLGLRKWQF DVWSDDVTLA NHMESGGVAG RVHITKQTLD FLGDKFEVEQ GEGGNRDAYL 490 500 510 520 530 540 ADHKVESYLI VPPKPAYTYS VPRVVECIEQ NDPSPTTEET KEIKETDQSH EATDVADVLL 550 560 570 580 590 600 PVTVAPPPAI VDEKMSPTSI NSQEAPLHAP LASAASMSIK ELSEEEDEAD EATAVTEPLM 610 620 630 640 650 660 HRDQDGKNDK EPKANGGHRG SGDSAASESV AKSAALSLPA DDLLSMSGSE SGISNSGAQA 670 680 690 700 710 720 QSSNPASVTP TAAAPAGGAA SNSLTVAEAP ERSRRKLSVQ GLMSFADRRR SSGAFIEGRK 730 740 750 760 770 780 LSIHSGESFR SHAGHVTRNR PSSKMTKYVE CWGADRPFAN IAESKLVKNI GLASIAMIES 790 800 810 820 830 840 NLLPPERKCF NFNFFGPPTE LKPFTMWYRN TPREAMYRAQ PDTHFRFDLI CAFVLFLSLA 850 860 870 880 890 900 VVQLIVIELN LALLGSLLAS FVSLALFLYL SNMSVPDVHA STTERNGPGQ VVASSRYLRL 910 920 930 940 950 960 AMFVVVNILI SSCAVFSVIN YTVPDGVSKE PSSNQTILES NFSSVFVNST LEDVQLWEID 970 980 990 1000 1010 1020 YAIPIAPVFL YCCAISLAAI SAFLRSGFIL KLIAMLVAVI AQVTVLGYSD LFEMYNDANI 1030 1040 1050 1060 1070 1080 THGLPLEIKG FLLLLVIILV LHTLDRQGEY VARTDFLWKA KLKVEQEEVE TMRGINKILL 1090 1100 1110 1120 1130 1140 ENILPAHVAT HFLHLERSTE LYHESYSCVA VMFASIPNYK EFYDETDVNK QGLECLRLLN 1150 1160 1170 1180 1190 1200 EIICDFDKLL LKPKFSGIEK IKTIASTYMC ASGLRPGKED GATDEKRTEE HNVVILVEFA 1210 1220 1230 1240 1250 1260 IALMSILDSI NRESFQRFRL RIGLNHGPVI AGVIGAQKPQ YDIWSNTVNV ASRMDSCGVM 1270 1280 1290 1300 GRLQTTENTA KILMTAGYEC ECRGLTYVKG KGNLVTYFVK TPFDGKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila learning and memory gene rutabaga encodes a Ca2+/Calmodulin-responsive adenylyl cyclase." Levin L.R., Han P.-L., Hwang P.M., Feinstein P.G., Davis R.L., Reed R.R. Cell 68:479-489(1992) [PubMed: 1739965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 434-1307. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093454 mRNA. Translation: AAC62509.1. AE014296 Genomic DNA. Translation: AAF49089.3. AY119547 mRNA. Translation: AAM50201.1. Different initiation.
PIR	B42088. T13158.
RefSeq	NP_524173.2.
UniGene	Dm.2181
3D structure databases
SMR	Q9VW60. Positions 305-491, 1101-1301.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VW60.
Genome annotation databases
Ensembl	FBtr0074897; FBpp0074666; FBgn0004852; Drosophila melanogaster. [Genome view]
GeneID	40180.
KEGG	dme:Dmel_CG7978.
NMPDR	fig|7227.3.peg.10642.
Organism-specific databases
CTD	40180.
FlyBase	FBgn0004852. Ac76E.
Phylogenomic databases
eggNOG	inNOG07691.
InParanoid	Q9VW60.
OMA	ANIAESK.
OrthoDB	EOG9QC1BX.
PhylomeDB	Q9VW60.
Enzyme and pathway databases
BRENDA	4.6.1.1. 48.
Gene expression databases
GermOnline	CG7978. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 2 hits. [Graphical view]
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 2 hits. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	817433.

Entry information

Entry name

ADCY2_DROME

Accession

Primary (citable) accession number: Q9VW60
Secondary accession number(s): O77247, Q8MRK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project