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Q9VVX0

- GEMI2_DROME

UniProt

Q9VVX0 - GEMI2_DROME

Protein

Protein Gemin2

Gene

Gem2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm, is required for pre-mRNA splicing in the nucleus and acts as a chaperone that discriminates target and non-target RNAs of Sm proteins.2 Publications

    GO - Biological processi

    1. adult locomotory behavior Source: FlyBase
    2. flight behavior Source: FlyBase
    3. spliceosomal complex assembly Source: InterPro
    4. spliceosomal snRNP assembly Source: UniProtKB

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Gemin2Imported
    Gene namesi
    Name:Gem2Imported
    ORF Names:CG10419
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0036850. Gem2.

    Subcellular locationi

    Cytoplasm 2 Publications
    Note: Component of U bodies.2 Publications

    GO - Cellular componenti

    1. cytoplasmic U snRNP body Source: FlyBase
    2. SmD-containing SMN-Sm protein complex Source: UniProtKB
    3. SMN-Gemin2 complex Source: UniProtKB
    4. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245Protein Gemin2PRO_0000424375Add
    BLAST

    Proteomic databases

    PRIDEiQ9VVX0.

    Expressioni

    Tissue specificityi

    Expressed in nurse cells and oocytes.1 Publication

    Gene expression databases

    BgeeiQ9VVX0.

    Interactioni

    Subunit structurei

    Part of the core SMN complex, which seems to be composed of Smn and Gem2 only. The SMN complex associates with the entire set of spliceosomal snRNP Sm proteins, SmB, SmD1, SmD2, SmD3, SmE, SmF and SmG, and with the snRNP-specific proteins snRNP-U1-70K, U2A, snf/U1A and U5-116KD.2 Publications

    Protein-protein interaction databases

    IntActiQ9VVX0. 3 interactions.
    MINTiMINT-1625145.
    STRINGi7227.FBpp0074799.

    Structurei

    Secondary structure

    1
    245
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi32 – 343
    Helixi35 – 4612
    Beta strandi56 – 583
    Helixi85 – 11127
    Helixi116 – 1183
    Helixi127 – 1359
    Helixi140 – 1434
    Helixi148 – 16013
    Beta strandi171 – 1744
    Helixi175 – 18713
    Helixi194 – 21219
    Helixi219 – 23416
    Helixi242 – 2443

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VU2X-ray3.102/F/N/V/d/l/t1-245[»]
    1VU3X-ray3.10F/N/V/d/l/t1-245[»]
    4F77X-ray3.102/F/N/V/d/l/t1-245[»]
    ProteinModelPortaliQ9VVX0.
    SMRiQ9VVX0. Positions 30-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the gemin-2 family.Sequence Analysis

    Phylogenomic databases

    GeneTreeiENSGT00390000013814.
    InParanoidiQ9VVX0.
    KOiK13130.
    OMAiCLGERLY.
    PhylomeDBiQ9VVX0.

    Family and domain databases

    InterProiIPR007022. GEMIN2.
    IPR017364. GEMIN2_metazoa.
    [Graphical view]
    PANTHERiPTHR12794. PTHR12794. 1 hit.
    PIRSFiPIRSF038038. SMN_Gemin2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9VVX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQHEPEDQTF QLQALEICEP DSSFDPQKPP ESGEEYLMHM FYERKRCPAV    50
    VTKRSSKIRN NTGNTTLEML DNPELPPFKC LLPTPEWRDE QVKSFQAARS 100
    QVLVLRKELA NNNYDQSGEP PLTSDQEKWK EFCRNQQPLL STLLHLTQND 150
    LELLLEMLSK WLQDPNTTVD LLHDVWLARW LYATLVCLHL PLEPHVFSTL 200
    RYIARTCIHL RNQLKEDEVQ RAAPYNLLLT LTVQVFAQND FKDYI 245
    Length:245
    Mass (Da):28,751
    Last modified:May 1, 2000 - v1
    Checksum:i1E9A700ADD97EDFF
    GO

    Sequence cautioni

    The sequence AAL39848.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014296 Genomic DNA. Translation: AAF49187.1.
    AE014296 Genomic DNA. Translation: AGB94727.1.
    AY069703 mRNA. Translation: AAL39848.2. Different initiation.
    RefSeqiNP_001262034.1. NM_001275105.1.
    NP_649092.1. NM_140835.4.
    UniGeneiDm.1995.

    Genome annotation databases

    EnsemblMetazoaiFBtr0075032; FBpp0074799; FBgn0036850.
    FBtr0330093; FBpp0303126; FBgn0036850.
    GeneIDi40087.
    KEGGidme:Dmel_CG10419.
    UCSCiCG10419-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014296 Genomic DNA. Translation: AAF49187.1 .
    AE014296 Genomic DNA. Translation: AGB94727.1 .
    AY069703 mRNA. Translation: AAL39848.2 . Different initiation.
    RefSeqi NP_001262034.1. NM_001275105.1.
    NP_649092.1. NM_140835.4.
    UniGenei Dm.1995.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VU2 X-ray 3.10 2/F/N/V/d/l/t 1-245 [» ]
    1VU3 X-ray 3.10 F/N/V/d/l/t 1-245 [» ]
    4F77 X-ray 3.10 2/F/N/V/d/l/t 1-245 [» ]
    ProteinModelPortali Q9VVX0.
    SMRi Q9VVX0. Positions 30-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9VVX0. 3 interactions.
    MINTi MINT-1625145.
    STRINGi 7227.FBpp0074799.

    Proteomic databases

    PRIDEi Q9VVX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0075032 ; FBpp0074799 ; FBgn0036850 .
    FBtr0330093 ; FBpp0303126 ; FBgn0036850 .
    GeneIDi 40087.
    KEGGi dme:Dmel_CG10419.
    UCSCi CG10419-RA. d. melanogaster.

    Organism-specific databases

    CTDi 40087.
    FlyBasei FBgn0036850. Gem2.

    Phylogenomic databases

    GeneTreei ENSGT00390000013814.
    InParanoidi Q9VVX0.
    KOi K13130.
    OMAi CLGERLY.
    PhylomeDBi Q9VVX0.

    Miscellaneous databases

    GenomeRNAii 40087.
    NextBioi 816928.

    Gene expression databases

    Bgeei Q9VVX0.

    Family and domain databases

    InterProi IPR007022. GEMIN2.
    IPR017364. GEMIN2_metazoa.
    [Graphical view ]
    PANTHERi PTHR12794. PTHR12794. 1 hit.
    PIRSFi PIRSF038038. SMN_Gemin2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BerkeleyImported.
    4. "Evolution of an RNP assembly system: a minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster."
      Kroiss M., Schultz J., Wiesner J., Chari A., Sickmann A., Fischer U.
      Proc. Natl. Acad. Sci. U.S.A. 105:10045-10050(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE SMN COMPLEX, INTERACTION WITH THE SPLICEOSOME USNRNP PROTEINS SNRNP-U1-70K, U2A, SNF/U1A AND U5-116KD, INTERACTION WITH THE SNRNP SM PROTEINS, SUBCELLULAR LOCATION.
    5. "Drosophila SMN complex proteins Gemin2, Gemin3, and Gemin5 are components of U bodies."
      Cauchi R.J., Sanchez-Pulido L., Liu J.L.
      Exp. Cell Res. 316:2354-2364(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Structural basis of assembly chaperone-mediated snRNP formation."
      Grimm C., Chari A., Pelz J.P., Kuper J., Kisker C., Diederichs K., Stark H., Schindelin H., Fischer U.
      Mol. Cell 49:692-703(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS), FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiGEMI2_DROME
    AccessioniPrimary (citable) accession number: Q9VVX0
    Secondary accession number(s): Q7K0M0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3