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Q9VVX0

- GEMI2_DROME

UniProt

Q9VVX0 - GEMI2_DROME

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Protein

Protein Gemin2

Gene

Gem2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm, is required for pre-mRNA splicing in the nucleus and acts as a chaperone that discriminates target and non-target RNAs of Sm proteins.2 Publications

GO - Biological processi

  1. adult locomotory behavior Source: FlyBase
  2. flight behavior Source: FlyBase
  3. spliceosomal complex assembly Source: InterPro
  4. spliceosomal snRNP assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Gemin2Imported
Gene namesi
Name:Gem2Imported
ORF Names:CG10419
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0036850. Gem2.

Subcellular locationi

Cytoplasm 2 Publications
Note: Component of U bodies.2 Publications

GO - Cellular componenti

  1. cytoplasmic U snRNP body Source: FlyBase
  2. SmD-containing SMN-Sm protein complex Source: UniProtKB
  3. SMN-Gemin2 complex Source: UniProtKB
  4. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein Gemin2PRO_0000424375Add
BLAST

Proteomic databases

PRIDEiQ9VVX0.

Expressioni

Tissue specificityi

Expressed in nurse cells and oocytes.1 Publication

Gene expression databases

BgeeiQ9VVX0.

Interactioni

Subunit structurei

Part of the core SMN complex, which seems to be composed of Smn and Gem2 only. The SMN complex associates with the entire set of spliceosomal snRNP Sm proteins, SmB, SmD1, SmD2, SmD3, SmE, SmF and SmG, and with the snRNP-specific proteins snRNP-U1-70K, U2A, snf/U1A and U5-116KD.2 Publications

Protein-protein interaction databases

IntActiQ9VVX0. 3 interactions.
MINTiMINT-1625145.
STRINGi7227.FBpp0074799.

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 343Combined sources
Helixi35 – 4612Combined sources
Beta strandi56 – 583Combined sources
Helixi85 – 11127Combined sources
Helixi116 – 1183Combined sources
Helixi127 – 1359Combined sources
Helixi140 – 1434Combined sources
Helixi148 – 16013Combined sources
Beta strandi171 – 1744Combined sources
Helixi175 – 18713Combined sources
Helixi194 – 21219Combined sources
Helixi219 – 23416Combined sources
Helixi242 – 2443Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VU2X-ray3.102/F/N/V/d/l/t1-245[»]
1VU3X-ray3.10F/N/V/d/l/t1-245[»]
4F77X-ray3.102/F/N/V/d/l/t1-245[»]
ProteinModelPortaliQ9VVX0.
SMRiQ9VVX0. Positions 30-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gemin-2 family.Sequence Analysis

Phylogenomic databases

GeneTreeiENSGT00390000013814.
InParanoidiQ9VVX0.
KOiK13130.
OMAiCLGERLY.
PhylomeDBiQ9VVX0.

Family and domain databases

InterProiIPR007022. GEMIN2.
IPR017364. GEMIN2_metazoa.
[Graphical view]
PANTHERiPTHR12794. PTHR12794. 1 hit.
PIRSFiPIRSF038038. SMN_Gemin2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VVX0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQHEPEDQTF QLQALEICEP DSSFDPQKPP ESGEEYLMHM FYERKRCPAV
60 70 80 90 100
VTKRSSKIRN NTGNTTLEML DNPELPPFKC LLPTPEWRDE QVKSFQAARS
110 120 130 140 150
QVLVLRKELA NNNYDQSGEP PLTSDQEKWK EFCRNQQPLL STLLHLTQND
160 170 180 190 200
LELLLEMLSK WLQDPNTTVD LLHDVWLARW LYATLVCLHL PLEPHVFSTL
210 220 230 240
RYIARTCIHL RNQLKEDEVQ RAAPYNLLLT LTVQVFAQND FKDYI
Length:245
Mass (Da):28,751
Last modified:May 1, 2000 - v1
Checksum:i1E9A700ADD97EDFF
GO

Sequence cautioni

The sequence AAL39848.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49187.1.
AE014296 Genomic DNA. Translation: AGB94727.1.
AY069703 mRNA. Translation: AAL39848.2. Different initiation.
RefSeqiNP_001262034.1. NM_001275105.1.
NP_649092.1. NM_140835.4.
UniGeneiDm.1995.

Genome annotation databases

EnsemblMetazoaiFBtr0075032; FBpp0074799; FBgn0036850.
FBtr0330093; FBpp0303126; FBgn0036850.
GeneIDi40087.
KEGGidme:Dmel_CG10419.
UCSCiCG10419-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49187.1 .
AE014296 Genomic DNA. Translation: AGB94727.1 .
AY069703 mRNA. Translation: AAL39848.2 . Different initiation.
RefSeqi NP_001262034.1. NM_001275105.1.
NP_649092.1. NM_140835.4.
UniGenei Dm.1995.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VU2 X-ray 3.10 2/F/N/V/d/l/t 1-245 [» ]
1VU3 X-ray 3.10 F/N/V/d/l/t 1-245 [» ]
4F77 X-ray 3.10 2/F/N/V/d/l/t 1-245 [» ]
ProteinModelPortali Q9VVX0.
SMRi Q9VVX0. Positions 30-245.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9VVX0. 3 interactions.
MINTi MINT-1625145.
STRINGi 7227.FBpp0074799.

Proteomic databases

PRIDEi Q9VVX0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0075032 ; FBpp0074799 ; FBgn0036850 .
FBtr0330093 ; FBpp0303126 ; FBgn0036850 .
GeneIDi 40087.
KEGGi dme:Dmel_CG10419.
UCSCi CG10419-RA. d. melanogaster.

Organism-specific databases

CTDi 40087.
FlyBasei FBgn0036850. Gem2.

Phylogenomic databases

GeneTreei ENSGT00390000013814.
InParanoidi Q9VVX0.
KOi K13130.
OMAi CLGERLY.
PhylomeDBi Q9VVX0.

Miscellaneous databases

GenomeRNAii 40087.
NextBioi 816928.

Gene expression databases

Bgeei Q9VVX0.

Family and domain databases

InterProi IPR007022. GEMIN2.
IPR017364. GEMIN2_metazoa.
[Graphical view ]
PANTHERi PTHR12794. PTHR12794. 1 hit.
PIRSFi PIRSF038038. SMN_Gemin2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
  4. "Evolution of an RNP assembly system: a minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster."
    Kroiss M., Schultz J., Wiesner J., Chari A., Sickmann A., Fischer U.
    Proc. Natl. Acad. Sci. U.S.A. 105:10045-10050(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SMN COMPLEX, INTERACTION WITH THE SPLICEOSOME USNRNP PROTEINS SNRNP-U1-70K, U2A, SNF/U1A AND U5-116KD, INTERACTION WITH THE SNRNP SM PROTEINS, SUBCELLULAR LOCATION.
  5. "Drosophila SMN complex proteins Gemin2, Gemin3, and Gemin5 are components of U bodies."
    Cauchi R.J., Sanchez-Pulido L., Liu J.L.
    Exp. Cell Res. 316:2354-2364(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Structural basis of assembly chaperone-mediated snRNP formation."
    Grimm C., Chari A., Pelz J.P., Kuper J., Kisker C., Diederichs K., Stark H., Schindelin H., Fischer U.
    Mol. Cell 49:692-703(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS), FUNCTION, SUBUNIT.

Entry informationi

Entry nameiGEMI2_DROME
AccessioniPrimary (citable) accession number: Q9VVX0
Secondary accession number(s): Q7K0M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3