Q9VVT2 (INDY1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein I'm not dead yet

Alternative name(s):
INDY transporter protein
drIndy

Gene names

Name:	Indy
ORF Names:	CG3979

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines. Ref.1 Ref.6 Ref.7
Subcellular location	Basolateral cell membrane; Multi-pass membrane protein. Note: Basolateral membrane of cells in the midgut. Ref.7
Tissue specificity	In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae. Ref.6 Ref.7
Induction	Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide. Ref.7
Miscellaneous	The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function. Ref.6 Ref.7
Sequence similarities	Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]
Sequence caution	The sequence AAF73384.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	determination of adult lifespan Inferred from direct assay PubMed 19470468. Source: FlyBase regulation of sequestering of triglyceride Inferred from direct assay PubMed 19470468. Source: FlyBase sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	citrate transmembrane transporter activity Inferred from direct assay Ref.7. Source: UniProtKB pyruvate transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB succinate transmembrane transporter activity Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
spri	Q9W2U1	1	EBI-110127,EBI-99646

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VVT2-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9VVT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Protein I'm not dead yet		PRO_0000172499
Regions
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Transmembrane	270 – 290	21	Helical; Potential
	Transmembrane	329 – 349	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
	Transmembrane	457 – 477	21	Helical; Potential
	Transmembrane	509 – 529	21	Helical; Potential
	Transmembrane	540 – 560	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 18	18	Missing in isoform B.		VSP_029170

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (C) [UniParc]. Last modified November 13, 2007. Version 2. Checksum: ECA7857BB6B34414	FASTA	590	65,585
	10 20 30 40 50 60 MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP LLCLPVMLLN 70 80 90 100 110 120 EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG IMSSDQTCRL YFKDTLVMFM 130 140 150 160 170 180 GGIMVALAVE YCNLHKRLAL RVIQIVGCSP RRLHFGLIMV TMFLSMWISN AACTAMMCPI 190 200 210 220 230 240 IQAVLEELQA QGVCKINHEP QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG 250 260 270 280 290 300 TIIGTATNLT FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP 310 320 330 340 350 360 KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY FTRKPGIFLG 370 380 390 400 410 420 WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG GPVPTGPTPS LITWKFIQTK 430 440 450 460 470 480 VPWGLVFLLG GGFALAEGSK QSGMAKLIGN ALIGLKVLPN SVLLLVVILV AVFLTAFSSN 490 500 510 520 530 540 VAIANIIIPV LAEMSLAIEI HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM 550 560 570 580 590 AIAGIGPTII TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH
	Isoform B [UniParc]. Checksum: C021D77D006C5444	FASTA	572	63,553
	10 20 30 40 50 60 MEIEIGEQPQ PPVKCSNFFA NHWKGLVVFL VPLLCLPVML LNEGAEFRCM YLLLVMAIFW 70 80 90 100 110 120 VTEALPLYVT SMIPIVAFPI MGIMSSDQTC RLYFKDTLVM FMGGIMVALA VEYCNLHKRL 130 140 150 160 170 180 ALRVIQIVGC SPRRLHFGLI MVTMFLSMWI SNAACTAMMC PIIQAVLEEL QAQGVCKINH 190 200 210 220 230 240 EPQYQIVGGN KKNNEDEPPY PTKITLCYYL GIAYASSLGG CGTIIGTATN LTFKGIYEAR 250 260 270 280 290 300 FKNSTEQMDF PTFMFYSVPS MLVYTLLTFV FLQWHFMGLW RPKSKEAQEV QRGREGADVA 310 320 330 340 350 360 KKVIDQRYKD LGPMSIHEIQ VMILFIFMVV MYFTRKPGIF LGWADLLNSK DIRNSMPTIF 370 380 390 400 410 420 VVVMCFMLPA NYAFLRYCTR RGGPVPTGPT PSLITWKFIQ TKVPWGLVFL LGGGFALAEG 430 440 450 460 470 480 SKQSGMAKLI GNALIGLKVL PNSVLLLVVI LVAVFLTAFS SNVAIANIII PVLAEMSLAI 490 500 510 520 530 540 EIHPLYLILP AGLACSMAFH LPVSTPPNAL VAGYANIRTK DMAIAGIGPT IITIITLFVF 550 560 570 CQTWGLVVYP NLNSFPEWAQ IYAAAALGNK TH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates." Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V. Biochem. J. 367:313-319(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[5]	"Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism." Schmid K.J., Nigro L., Aquadro C.F., Tautz D. Genetics 153:1717-1729(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572. Strain: Canton-S. Tissue: Embryo.
[6]	"Extended life-span conferred by cotransporter gene mutations in Drosophila." Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L. Science 290:2137-2140(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
[7]	"Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy." Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L. Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF509505 mRNA. Translation: AAN86815.1. AE014296 Genomic DNA. Translation: AAF49226.1. AE014296 Genomic DNA. Translation: AAF49227.2. AE014296 Genomic DNA. Translation: AAN11670.1. AY102686 mRNA. Translation: AAM27515.1. AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeq	NP_001163465.1. NM_001169994.2. NP_524150.1. NM_079426.4. NP_730363.2. NM_168778.2. NP_730364.1. NM_168779.2.
UniGene	Dm.2528.
3D structure databases
ProteinModelPortal	Q9VVT2.
SMR	Q9VVT2. Positions 53-563.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18443N.
IntAct	Q9VVT2. 1 interaction.
MINT	MINT-800124.
Protein family/group databases
TCDB	2.A.47.1.10. divalent anion:Na+ symporter (DASS) family.
Proteomic databases
PaxDb	Q9VVT2.
PRIDE	Q9VVT2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075081; FBpp0074848; FBgn0036816.
GeneID	40049.
KEGG	dme:Dmel_CG3979.
Organism-specific databases
CTD	40049.
FlyBase	FBgn0036816. Indy.
Phylogenomic databases
eggNOG	COG0471.
GeneTree	ENSGT00550000074480.
InParanoid	Q9VVT2.
KO	K14445.
OMA	MAIAIEI.
OrthoDB	EOG4J6Q6C.
PhylomeDB	Q9VVT2.
Gene expression databases
Bgee	Q9VVT2.
GermOnline	CG3979. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001898. Na/sul_symport. [Graphical view]
Pfam	PF00939. Na_sulph_symp. 1 hit. [Graphical view]
PROSITE	PS01271. NA_SULFATE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Indy. drosophila.
GenomeRNAi	40049.
NextBio	816740.

Entry information

Entry name

INDY1_DROME

Accession

Primary (citable) accession number: Q9VVT2
Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project