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Q9VVT2 (INDY1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein I'm not dead yet
Alternative name(s):
INDY transporter protein
drIndy
Gene names
Name:Indy
ORF Names:CG3979
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines. Ref.1 Ref.6 Ref.7

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein. Note: Basolateral membrane of cells in the midgut. Ref.7

Tissue specificity

In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae. Ref.6 Ref.7

Induction

Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide. Ref.7

Miscellaneous

The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function. Ref.6 Ref.7

Sequence similarities

Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]

Sequence caution

The sequence AAF73384.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcitrate transport

Inferred from direct assay Ref.1Ref.7. Source: UniProtKB

determination of adult lifespan

Inferred from direct assay PubMed 19470468. Source: FlyBase

pyruvate transmembrane transport

Inferred from direct assay Ref.1. Source: GOC

pyruvate transport

Inferred from direct assay Ref.1. Source: UniProtKB

regulation of sequestering of triglyceride

Inferred from direct assay PubMed 19470468. Source: FlyBase

sodium ion transport

Inferred from electronic annotation. Source: InterPro

succinate transmembrane transport

Inferred from direct assay Ref.1Ref.7. Source: GOC

succinate transport

Inferred from direct assay Ref.1Ref.7. Source: UniProtKB

   Cellular_componentbasolateral plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Inferred from direct assay Ref.7. Source: UniProtKB

plasma membrane

Inferred from direct assay Ref.7PubMed 20462449. Source: FlyBase

   Molecular_functioncitrate transmembrane transporter activity

Inferred from direct assay Ref.7. Source: UniProtKB

pyruvate transmembrane transporter activity

Inferred from direct assay Ref.1. Source: UniProtKB

succinate transmembrane transporter activity

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

spriQ9W2U11EBI-110127,EBI-99646

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9VVT2-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q9VVT2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Protein I'm not dead yet
PRO_0000172499

Regions

Transmembrane43 – 6321Helical; Potential
Transmembrane82 – 10221Helical; Potential
Transmembrane115 – 13521Helical; Potential
Transmembrane153 – 17321Helical; Potential
Transmembrane224 – 24421Helical; Potential
Transmembrane270 – 29021Helical; Potential
Transmembrane329 – 34921Helical; Potential
Transmembrane373 – 39321Helical; Potential
Transmembrane457 – 47721Helical; Potential
Transmembrane509 – 52921Helical; Potential
Transmembrane540 – 56021Helical; Potential

Natural variations

Alternative sequence1 – 1818Missing in isoform B.
VSP_029170

Sequences

Sequence LengthMass (Da)Tools
Isoform A (C) [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: ECA7857BB6B34414

FASTA59065,585
        10         20         30         40         50         60 
MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP LLCLPVMLLN 

        70         80         90        100        110        120 
EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG IMSSDQTCRL YFKDTLVMFM 

       130        140        150        160        170        180 
GGIMVALAVE YCNLHKRLAL RVIQIVGCSP RRLHFGLIMV TMFLSMWISN AACTAMMCPI 

       190        200        210        220        230        240 
IQAVLEELQA QGVCKINHEP QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG 

       250        260        270        280        290        300 
TIIGTATNLT FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP 

       310        320        330        340        350        360 
KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY FTRKPGIFLG 

       370        380        390        400        410        420 
WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG GPVPTGPTPS LITWKFIQTK 

       430        440        450        460        470        480 
VPWGLVFLLG GGFALAEGSK QSGMAKLIGN ALIGLKVLPN SVLLLVVILV AVFLTAFSSN 

       490        500        510        520        530        540 
VAIANIIIPV LAEMSLAIEI HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM 

       550        560        570        580        590 
AIAGIGPTII TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH 

« Hide

Isoform B [UniParc].

Checksum: C021D77D006C5444
Show »

FASTA57263,553

References

« Hide 'large scale' references
[1]"Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates."
Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V.
Biochem. J. 367:313-319(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[5]"Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism."
Schmid K.J., Nigro L., Aquadro C.F., Tautz D.
Genetics 153:1717-1729(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572.
Strain: Canton-S.
Tissue: Embryo.
[6]"Extended life-span conferred by cotransporter gene mutations in Drosophila."
Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L.
Science 290:2137-2140(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
[7]"Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy."
Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L.
Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF509505 mRNA. Translation: AAN86815.1.
AE014296 Genomic DNA. Translation: AAF49226.1.
AE014296 Genomic DNA. Translation: AAF49227.2.
AE014296 Genomic DNA. Translation: AAN11670.1.
AY102686 mRNA. Translation: AAM27515.1.
AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeqNP_001163465.1. NM_001169994.2. [Q9VVT2-2]
NP_524150.1. NM_079426.4. [Q9VVT2-2]
NP_730363.2. NM_168778.2. [Q9VVT2-1]
NP_730364.1. NM_168779.2. [Q9VVT2-2]
UniGeneDm.2528.

3D structure databases

ProteinModelPortalQ9VVT2.
SMRQ9VVT2. Positions 70-248, 411-545.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid65330. 4 interactions.
DIPDIP-18443N.
IntActQ9VVT2. 1 interaction.
MINTMINT-800124.

Protein family/group databases

TCDB2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

Proteomic databases

PaxDbQ9VVT2.
PRIDEQ9VVT2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0075081; FBpp0074848; FBgn0036816. [Q9VVT2-1]
GeneID40049.
KEGGdme:Dmel_CG3979.

Organism-specific databases

CTD40049.
FlyBaseFBgn0036816. Indy.

Phylogenomic databases

eggNOGCOG0471.
GeneTreeENSGT00550000074480.
InParanoidQ9VVT2.
KOK14445.
OMAMFIIPSK.
OrthoDBEOG72NRPP.
PhylomeDBQ9VVT2.

Gene expression databases

BgeeQ9VVT2.

Family and domain databases

InterProIPR001898. Na/sul_symport.
[Graphical view]
PfamPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEPS01271. NA_SULFATE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIndy. drosophila.
GenomeRNAi40049.
NextBio816740.

Entry information

Entry nameINDY1_DROME
AccessionPrimary (citable) accession number: Q9VVT2
Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 13, 2007
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase