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Protein

Protein I'm not dead yet

Gene

Indy

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.3 Publications

GO - Molecular functioni

  1. citrate transmembrane transporter activity Source: UniProtKB
  2. pyruvate transmembrane transporter activity Source: UniProtKB
  3. succinate transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  1. citrate transport Source: UniProtKB
  2. determination of adult lifespan Source: FlyBase
  3. pyruvate transmembrane transport Source: GOC
  4. pyruvate transport Source: UniProtKB
  5. regulation of sequestering of triglyceride Source: FlyBase
  6. sodium ion transport Source: InterPro
  7. succinate transmembrane transport Source: GOC
  8. succinate transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_233051. Sodium-coupled sulphate, di- and tri-carboxylate transporters.

Protein family/group databases

TCDBi2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein I'm not dead yet
Alternative name(s):
INDY transporter protein
drIndy
Gene namesi
Name:Indy
ORF Names:CG3979
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0036816. Indy.

Subcellular locationi

Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Basolateral membrane of cells in the midgut.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei43 – 6321HelicalSequence AnalysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence AnalysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence AnalysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence AnalysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence AnalysisAdd
BLAST
Transmembranei373 – 39321HelicalSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST
Transmembranei509 – 52921HelicalSequence AnalysisAdd
BLAST
Transmembranei540 – 56021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Protein I'm not dead yetPRO_0000172499Add
BLAST

Proteomic databases

PaxDbiQ9VVT2.
PRIDEiQ9VVT2.

Expressioni

Tissue specificityi

In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae.2 Publications

Inductioni

Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide.1 Publication

Gene expression databases

BgeeiQ9VVT2.
ExpressionAtlasiQ9VVT2. differential.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
spriQ9W2U11EBI-110127,EBI-99646

Protein-protein interaction databases

BioGridi65330. 4 interactions.
DIPiDIP-18443N.
IntActiQ9VVT2. 2 interactions.
MINTiMINT-800124.

Structurei

3D structure databases

ProteinModelPortaliQ9VVT2.
SMRiQ9VVT2. Positions 70-248, 411-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0471.
GeneTreeiENSGT00550000074480.
InParanoidiQ9VVT2.
KOiK14445.
OMAiMFIIPSK.
OrthoDBiEOG72NRPP.
PhylomeDBiQ9VVT2.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEiPS01271. NA_SULFATE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9VVT2-1) [UniParc]FASTAAdd to basket

Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP
60 70 80 90 100
LLCLPVMLLN EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG
110 120 130 140 150
IMSSDQTCRL YFKDTLVMFM GGIMVALAVE YCNLHKRLAL RVIQIVGCSP
160 170 180 190 200
RRLHFGLIMV TMFLSMWISN AACTAMMCPI IQAVLEELQA QGVCKINHEP
210 220 230 240 250
QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG TIIGTATNLT
260 270 280 290 300
FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP
310 320 330 340 350
KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY
360 370 380 390 400
FTRKPGIFLG WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG
410 420 430 440 450
GPVPTGPTPS LITWKFIQTK VPWGLVFLLG GGFALAEGSK QSGMAKLIGN
460 470 480 490 500
ALIGLKVLPN SVLLLVVILV AVFLTAFSSN VAIANIIIPV LAEMSLAIEI
510 520 530 540 550
HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM AIAGIGPTII
560 570 580 590
TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH
Length:590
Mass (Da):65,585
Last modified:November 13, 2007 - v2
Checksum:iECA7857BB6B34414
GO
Isoform B (identifier: Q9VVT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Note: No experimental confirmation available.

Show »
Length:572
Mass (Da):63,553
Checksum:iC021D77D006C5444
GO

Sequence cautioni

The sequence AAF73384.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1818Missing in isoform B. CuratedVSP_029170Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509505 mRNA. Translation: AAN86815.1.
AE014296 Genomic DNA. Translation: AAF49226.1.
AE014296 Genomic DNA. Translation: AAF49227.2.
AE014296 Genomic DNA. Translation: AAN11670.1.
AY102686 mRNA. Translation: AAM27515.1.
AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeqiNP_001163465.1. NM_001169994.2. [Q9VVT2-2]
NP_524150.1. NM_079426.4. [Q9VVT2-2]
NP_730363.2. NM_168778.2. [Q9VVT2-1]
NP_730364.1. NM_168779.2. [Q9VVT2-2]
UniGeneiDm.2528.

Genome annotation databases

EnsemblMetazoaiFBtr0075081; FBpp0074848; FBgn0036816. [Q9VVT2-1]
GeneIDi40049.
KEGGidme:Dmel_CG3979.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509505 mRNA. Translation: AAN86815.1.
AE014296 Genomic DNA. Translation: AAF49226.1.
AE014296 Genomic DNA. Translation: AAF49227.2.
AE014296 Genomic DNA. Translation: AAN11670.1.
AY102686 mRNA. Translation: AAM27515.1.
AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeqiNP_001163465.1. NM_001169994.2. [Q9VVT2-2]
NP_524150.1. NM_079426.4. [Q9VVT2-2]
NP_730363.2. NM_168778.2. [Q9VVT2-1]
NP_730364.1. NM_168779.2. [Q9VVT2-2]
UniGeneiDm.2528.

3D structure databases

ProteinModelPortaliQ9VVT2.
SMRiQ9VVT2. Positions 70-248, 411-545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65330. 4 interactions.
DIPiDIP-18443N.
IntActiQ9VVT2. 2 interactions.
MINTiMINT-800124.

Protein family/group databases

TCDBi2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

Proteomic databases

PaxDbiQ9VVT2.
PRIDEiQ9VVT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075081; FBpp0074848; FBgn0036816. [Q9VVT2-1]
GeneIDi40049.
KEGGidme:Dmel_CG3979.

Organism-specific databases

CTDi40049.
FlyBaseiFBgn0036816. Indy.

Phylogenomic databases

eggNOGiCOG0471.
GeneTreeiENSGT00550000074480.
InParanoidiQ9VVT2.
KOiK14445.
OMAiMFIIPSK.
OrthoDBiEOG72NRPP.
PhylomeDBiQ9VVT2.

Enzyme and pathway databases

ReactomeiREACT_233051. Sodium-coupled sulphate, di- and tri-carboxylate transporters.

Miscellaneous databases

ChiTaRSiIndy. fly.
GenomeRNAii40049.
NextBioi816740.

Gene expression databases

BgeeiQ9VVT2.
ExpressionAtlasiQ9VVT2. differential.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEiPS01271. NA_SULFATE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates."
    Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V.
    Biochem. J. 367:313-319(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley1 Publication.
    Tissue: Embryo1 Publication.
  5. "Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism."
    Schmid K.J., Nigro L., Aquadro C.F., Tautz D.
    Genetics 153:1717-1729(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572.
    Strain: Canton-SImported.
    Tissue: Embryo.
  6. "Extended life-span conferred by cotransporter gene mutations in Drosophila."
    Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L.
    Science 290:2137-2140(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
  7. "Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy."
    Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L.
    Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiINDY1_DROME
AccessioniPrimary (citable) accession number: Q9VVT2
Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.