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Q9VVT2

- INDY1_DROME

UniProt

Q9VVT2 - INDY1_DROME

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Protein
Protein I'm not dead yet
Gene
Indy, CG3979
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.3 Publications

GO - Molecular functioni

  1. citrate transmembrane transporter activity Source: UniProtKB
  2. pyruvate transmembrane transporter activity Source: UniProtKB
  3. succinate transmembrane transporter activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. citrate transport Source: UniProtKB
  2. determination of adult lifespan Source: FlyBase
  3. pyruvate transmembrane transport Source: GOC
  4. pyruvate transport Source: UniProtKB
  5. regulation of sequestering of triglyceride Source: FlyBase
  6. sodium ion transport Source: InterPro
  7. succinate transmembrane transport Source: GOC
  8. succinate transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein I'm not dead yet
Alternative name(s):
INDY transporter protein
drIndy
Gene namesi
Name:Indy
ORF Names:CG3979
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0036816. Indy.

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein
Note: Basolateral membrane of cells in the midgut.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei43 – 6321Helical; Reviewed prediction
Add
BLAST
Transmembranei82 – 10221Helical; Reviewed prediction
Add
BLAST
Transmembranei115 – 13521Helical; Reviewed prediction
Add
BLAST
Transmembranei153 – 17321Helical; Reviewed prediction
Add
BLAST
Transmembranei224 – 24421Helical; Reviewed prediction
Add
BLAST
Transmembranei270 – 29021Helical; Reviewed prediction
Add
BLAST
Transmembranei329 – 34921Helical; Reviewed prediction
Add
BLAST
Transmembranei373 – 39321Helical; Reviewed prediction
Add
BLAST
Transmembranei457 – 47721Helical; Reviewed prediction
Add
BLAST
Transmembranei509 – 52921Helical; Reviewed prediction
Add
BLAST
Transmembranei540 – 56021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Protein I'm not dead yet
PRO_0000172499Add
BLAST

Proteomic databases

PaxDbiQ9VVT2.
PRIDEiQ9VVT2.

Expressioni

Tissue specificityi

In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae.2 Publications

Inductioni

Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide.1 Publication

Gene expression databases

BgeeiQ9VVT2.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
spriQ9W2U11EBI-110127,EBI-99646

Protein-protein interaction databases

BioGridi65330. 4 interactions.
DIPiDIP-18443N.
IntActiQ9VVT2. 1 interaction.
MINTiMINT-800124.

Structurei

3D structure databases

ProteinModelPortaliQ9VVT2.
SMRiQ9VVT2. Positions 70-248, 411-545.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0471.
GeneTreeiENSGT00550000074480.
InParanoidiQ9VVT2.
KOiK14445.
OMAiMFIIPSK.
OrthoDBiEOG72NRPP.
PhylomeDBiQ9VVT2.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEiPS01271. NA_SULFATE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q9VVT2-1) [UniParc]FASTAAdd to Basket

Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP    50
LLCLPVMLLN EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG 100
IMSSDQTCRL YFKDTLVMFM GGIMVALAVE YCNLHKRLAL RVIQIVGCSP 150
RRLHFGLIMV TMFLSMWISN AACTAMMCPI IQAVLEELQA QGVCKINHEP 200
QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG TIIGTATNLT 250
FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP 300
KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY 350
FTRKPGIFLG WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG 400
GPVPTGPTPS LITWKFIQTK VPWGLVFLLG GGFALAEGSK QSGMAKLIGN 450
ALIGLKVLPN SVLLLVVILV AVFLTAFSSN VAIANIIIPV LAEMSLAIEI 500
HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM AIAGIGPTII 550
TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH 590
Length:590
Mass (Da):65,585
Last modified:November 13, 2007 - v2
Checksum:iECA7857BB6B34414
GO
Isoform B (identifier: Q9VVT2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Note: No experimental confirmation available.

Show »
Length:572
Mass (Da):63,553
Checksum:iC021D77D006C5444
GO

Sequence cautioni

The sequence AAF73384.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1818Missing in isoform B.
VSP_029170Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF509505 mRNA. Translation: AAN86815.1.
AE014296 Genomic DNA. Translation: AAF49226.1.
AE014296 Genomic DNA. Translation: AAF49227.2.
AE014296 Genomic DNA. Translation: AAN11670.1.
AY102686 mRNA. Translation: AAM27515.1.
AF217399 mRNA. Translation: AAF73384.1. Different initiation.
RefSeqiNP_001163465.1. NM_001169994.2. [Q9VVT2-2]
NP_524150.1. NM_079426.4. [Q9VVT2-2]
NP_730363.2. NM_168778.2. [Q9VVT2-1]
NP_730364.1. NM_168779.2. [Q9VVT2-2]
UniGeneiDm.2528.

Genome annotation databases

EnsemblMetazoaiFBtr0075081; FBpp0074848; FBgn0036816. [Q9VVT2-1]
GeneIDi40049.
KEGGidme:Dmel_CG3979.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF509505 mRNA. Translation: AAN86815.1 .
AE014296 Genomic DNA. Translation: AAF49226.1 .
AE014296 Genomic DNA. Translation: AAF49227.2 .
AE014296 Genomic DNA. Translation: AAN11670.1 .
AY102686 mRNA. Translation: AAM27515.1 .
AF217399 mRNA. Translation: AAF73384.1 . Different initiation.
RefSeqi NP_001163465.1. NM_001169994.2. [Q9VVT2-2 ]
NP_524150.1. NM_079426.4. [Q9VVT2-2 ]
NP_730363.2. NM_168778.2. [Q9VVT2-1 ]
NP_730364.1. NM_168779.2. [Q9VVT2-2 ]
UniGenei Dm.2528.

3D structure databases

ProteinModelPortali Q9VVT2.
SMRi Q9VVT2. Positions 70-248, 411-545.
ModBasei Search...

Protein-protein interaction databases

BioGridi 65330. 4 interactions.
DIPi DIP-18443N.
IntActi Q9VVT2. 1 interaction.
MINTi MINT-800124.

Protein family/group databases

TCDBi 2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

Proteomic databases

PaxDbi Q9VVT2.
PRIDEi Q9VVT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0075081 ; FBpp0074848 ; FBgn0036816 . [Q9VVT2-1 ]
GeneIDi 40049.
KEGGi dme:Dmel_CG3979.

Organism-specific databases

CTDi 40049.
FlyBasei FBgn0036816. Indy.

Phylogenomic databases

eggNOGi COG0471.
GeneTreei ENSGT00550000074480.
InParanoidi Q9VVT2.
KOi K14445.
OMAi MFIIPSK.
OrthoDBi EOG72NRPP.
PhylomeDBi Q9VVT2.

Miscellaneous databases

ChiTaRSi Indy. drosophila.
GenomeRNAii 40049.
NextBioi 816740.

Gene expression databases

Bgeei Q9VVT2.

Family and domain databases

InterProi IPR001898. Na/sul_symport.
[Graphical view ]
Pfami PF00939. Na_sulph_symp. 1 hit.
[Graphical view ]
PROSITEi PS01271. NA_SULFATE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates."
    Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V.
    Biochem. J. 367:313-319(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism."
    Schmid K.J., Nigro L., Aquadro C.F., Tautz D.
    Genetics 153:1717-1729(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572.
    Strain: Canton-S.
    Tissue: Embryo.
  6. "Extended life-span conferred by cotransporter gene mutations in Drosophila."
    Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L.
    Science 290:2137-2140(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
  7. "Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy."
    Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L.
    Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiINDY1_DROME
AccessioniPrimary (citable) accession number: Q9VVT2
Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 13, 2007
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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