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Q9VVT2

- INDY1_DROME

UniProt

Q9VVT2 - INDY1_DROME

Protein

Protein I'm not dead yet

Gene

Indy

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.3 Publications

    GO - Molecular functioni

    1. citrate transmembrane transporter activity Source: UniProtKB
    2. pyruvate transmembrane transporter activity Source: UniProtKB
    3. succinate transmembrane transporter activity Source: UniProtKB

    GO - Biological processi

    1. citrate transport Source: UniProtKB
    2. determination of adult lifespan Source: FlyBase
    3. pyruvate transmembrane transport Source: GOC
    4. pyruvate transport Source: UniProtKB
    5. regulation of sequestering of triglyceride Source: FlyBase
    6. sodium ion transport Source: InterPro
    7. succinate transmembrane transport Source: GOC
    8. succinate transport Source: UniProtKB

    Keywords - Biological processi

    Transport

    Protein family/group databases

    TCDBi2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein I'm not dead yet
    Alternative name(s):
    INDY transporter protein
    drIndy
    Gene namesi
    Name:Indy
    ORF Names:CG3979
    OrganismiDrosophila melanogaster (Fruit fly)Imported
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0036816. Indy.

    Subcellular locationi

    Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Basolateral membrane of cells in the midgut.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: UniProtKB
    3. plasma membrane Source: FlyBase

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590Protein I'm not dead yetPRO_0000172499Add
    BLAST

    Proteomic databases

    PaxDbiQ9VVT2.
    PRIDEiQ9VVT2.

    Expressioni

    Tissue specificityi

    In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the third segment of the antennae.2 Publications

    Inductioni

    Completely inhibited by DIDS. Modest but significant inhibition by phloretin or furosemide.1 Publication

    Gene expression databases

    BgeeiQ9VVT2.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    spriQ9W2U11EBI-110127,EBI-99646

    Protein-protein interaction databases

    BioGridi65330. 4 interactions.
    DIPiDIP-18443N.
    IntActiQ9VVT2. 1 interaction.
    MINTiMINT-800124.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VVT2.
    SMRiQ9VVT2. Positions 70-248, 411-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei43 – 6321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei224 – 24421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei270 – 29021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei329 – 34921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei373 – 39321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei509 – 52921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei540 – 56021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0471.
    GeneTreeiENSGT00550000074480.
    InParanoidiQ9VVT2.
    KOiK14445.
    OMAiMFIIPSK.
    OrthoDBiEOG72NRPP.
    PhylomeDBiQ9VVT2.

    Family and domain databases

    InterProiIPR001898. Na/sul_symport.
    [Graphical view]
    PfamiPF00939. Na_sulph_symp. 1 hit.
    [Graphical view]
    PROSITEiPS01271. NA_SULFATE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q9VVT2-1) [UniParc]FASTAAdd to Basket

    Also known as: C

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP    50
    LLCLPVMLLN EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG 100
    IMSSDQTCRL YFKDTLVMFM GGIMVALAVE YCNLHKRLAL RVIQIVGCSP 150
    RRLHFGLIMV TMFLSMWISN AACTAMMCPI IQAVLEELQA QGVCKINHEP 200
    QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG TIIGTATNLT 250
    FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP 300
    KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY 350
    FTRKPGIFLG WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG 400
    GPVPTGPTPS LITWKFIQTK VPWGLVFLLG GGFALAEGSK QSGMAKLIGN 450
    ALIGLKVLPN SVLLLVVILV AVFLTAFSSN VAIANIIIPV LAEMSLAIEI 500
    HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM AIAGIGPTII 550
    TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH 590
    Length:590
    Mass (Da):65,585
    Last modified:November 13, 2007 - v2
    Checksum:iECA7857BB6B34414
    GO
    Isoform B (identifier: Q9VVT2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-18: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:572
    Mass (Da):63,553
    Checksum:iC021D77D006C5444
    GO

    Sequence cautioni

    The sequence AAF73384.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1818Missing in isoform B. CuratedVSP_029170Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF509505 mRNA. Translation: AAN86815.1.
    AE014296 Genomic DNA. Translation: AAF49226.1.
    AE014296 Genomic DNA. Translation: AAF49227.2.
    AE014296 Genomic DNA. Translation: AAN11670.1.
    AY102686 mRNA. Translation: AAM27515.1.
    AF217399 mRNA. Translation: AAF73384.1. Different initiation.
    RefSeqiNP_001163465.1. NM_001169994.2. [Q9VVT2-2]
    NP_524150.1. NM_079426.4. [Q9VVT2-2]
    NP_730363.2. NM_168778.2. [Q9VVT2-1]
    NP_730364.1. NM_168779.2. [Q9VVT2-2]
    UniGeneiDm.2528.

    Genome annotation databases

    EnsemblMetazoaiFBtr0075081; FBpp0074848; FBgn0036816. [Q9VVT2-1]
    GeneIDi40049.
    KEGGidme:Dmel_CG3979.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF509505 mRNA. Translation: AAN86815.1 .
    AE014296 Genomic DNA. Translation: AAF49226.1 .
    AE014296 Genomic DNA. Translation: AAF49227.2 .
    AE014296 Genomic DNA. Translation: AAN11670.1 .
    AY102686 mRNA. Translation: AAM27515.1 .
    AF217399 mRNA. Translation: AAF73384.1 . Different initiation.
    RefSeqi NP_001163465.1. NM_001169994.2. [Q9VVT2-2 ]
    NP_524150.1. NM_079426.4. [Q9VVT2-2 ]
    NP_730363.2. NM_168778.2. [Q9VVT2-1 ]
    NP_730364.1. NM_168779.2. [Q9VVT2-2 ]
    UniGenei Dm.2528.

    3D structure databases

    ProteinModelPortali Q9VVT2.
    SMRi Q9VVT2. Positions 70-248, 411-545.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 65330. 4 interactions.
    DIPi DIP-18443N.
    IntActi Q9VVT2. 1 interaction.
    MINTi MINT-800124.

    Protein family/group databases

    TCDBi 2.A.47.1.10. the divalent anion:na(+) symporter (dass) family.

    Proteomic databases

    PaxDbi Q9VVT2.
    PRIDEi Q9VVT2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0075081 ; FBpp0074848 ; FBgn0036816 . [Q9VVT2-1 ]
    GeneIDi 40049.
    KEGGi dme:Dmel_CG3979.

    Organism-specific databases

    CTDi 40049.
    FlyBasei FBgn0036816. Indy.

    Phylogenomic databases

    eggNOGi COG0471.
    GeneTreei ENSGT00550000074480.
    InParanoidi Q9VVT2.
    KOi K14445.
    OMAi MFIIPSK.
    OrthoDBi EOG72NRPP.
    PhylomeDBi Q9VVT2.

    Miscellaneous databases

    ChiTaRSi Indy. drosophila.
    GenomeRNAii 40049.
    NextBioi 816740.

    Gene expression databases

    Bgeei Q9VVT2.

    Family and domain databases

    InterProi IPR001898. Na/sul_symport.
    [Graphical view ]
    Pfami PF00939. Na_sulph_symp. 1 hit.
    [Graphical view ]
    PROSITEi PS01271. NA_SULFATE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates."
      Inoue K., Fei Y.-J., Huang W., Zhuang L., Chen Z., Ganapathy V.
      Biochem. J. 367:313-319(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley1 Publication.
      Tissue: Embryo1 Publication.
    5. "Large number of replacement polymorphisms in rapidly evolving genes of Drosophila. Implications for genome-wide surveys of DNA polymorphism."
      Schmid K.J., Nigro L., Aquadro C.F., Tautz D.
      Genetics 153:1717-1729(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 366-572.
      Strain: Canton-SImported.
      Tissue: Embryo.
    6. "Extended life-span conferred by cotransporter gene mutations in Drosophila."
      Rogina B., Reenan R.A., Nilsen S.P., Helfand S.L.
      Science 290:2137-2140(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY.
    7. "Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy."
      Knauf F., Rogina B., Jiang Z., Aronson P.S., Helfand S.L.
      Proc. Natl. Acad. Sci. U.S.A. 99:14315-14319(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiINDY1_DROME
    AccessioniPrimary (citable) accession number: Q9VVT2
    Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Miscellaneous

    The life-extending effect of mutations is likely caused by an alteration in energy balance caused by a decrease in transport function.2 Publications

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3