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Q9VVR1

- NOT_DROME

UniProt

Q9VVR1 - NOT_DROME

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Protein

Ubiquitin carboxyl-terminal hydrolase nonstop

Gene

not

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of histone H2B, thereby acting as a coactivator in a large subset of genes. Required to counteract heterochromatin silencing. Controls the development of neuronal connectivity in visual system by being required for accurate axon targeting in the optic lobe. Required for expression of ecdysone-induced genes such as br/broad.3 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei406 – 4061NucleophilePROSITE-ProRule annotation
Active sitei689 – 6891Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri283 – 34260UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. axon guidance Source: FlyBase
  2. axon target recognition Source: FlyBase
  3. chromatin modification Source: UniProtKB
  4. chromatin organization Source: FlyBase
  5. glial cell development Source: FlyBase
  6. glial cell migration Source: FlyBase
  7. histone deubiquitination Source: FlyBase
  8. positive regulation of transcription, DNA-templated Source: UniProtKB
  9. protein deubiquitination Source: FlyBase
  10. regulation of transcription, DNA-templated Source: FlyBase
  11. transcription, DNA-templated Source: UniProtKB-KW
  12. ubiquitin-dependent protein catabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.095.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase nonstop (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme nonstop
Ubiquitin thioesterase nonstop
Ubiquitin-specific-processing protease nonstop
Gene namesi
Name:not
ORF Names:CG4166
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0013717. not.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: FlyBase
  2. SAGA complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in the number and migration of glial cells located within the lamina plexus of the developing eye; the lack of glial cells causing mistargeting of the R1-R6 axons in the optic lobe. Lamina neuron development is normal.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Ubiquitin carboxyl-terminal hydrolase nonstopPRO_0000367514Add
BLAST

Proteomic databases

PaxDbiQ9VVR1.
PRIDEiQ9VVR1.

Expressioni

Tissue specificityi

Expressed in the optic lobe and central brain. Highly expressed in the lamina precursor cells but not in differentiated lamina neurons. Also expressed in marginal, epithelial and medulla glial cells adjacent to the lamina plexus.1 Publication

Gene expression databases

BgeeiQ9VVR1.

Interactioni

Subunit structurei

Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.1 Publication

Protein-protein interaction databases

BioGridi65311. 5 interactions.
IntActiQ9VVR1. 4 interactions.
MINTiMINT-6179419.
STRINGi7227.FBpp0288698.

Structurei

3D structure databases

ProteinModelPortaliQ9VVR1.
SMRiQ9VVR1. Positions 296-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini397 – 730334USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri283 – 34260UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
InParanoidiQ9VVR1.
KOiK11366.
OrthoDBiEOG7FR7G7.
PhylomeDBiQ9VVR1.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR015880. Znf_C2H2-like.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VVR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFQYSYIRKI PMPTSEYDFA TGHTDSCLLP THMRACVCVC VQCEIETRKA
60 70 80 90 100
SEKSQKFTKK KPNYFQNWAA RRRTAKVAKI RRKQRKCDKA FVPLQSSAKR
110 120 130 140 150
AKTKENSVDS NTSVFSSSSS SSSGRSFGRG TERWAVGAEA GRGTAENETL
160 170 180 190 200
AVAAAAATTT NAGRFITADL IKTVNSDKQR DRVAKGHRDA KNPLDATKHL
210 220 230 240 250
RLSDCLRTVQ KLAIELESAD AGSDASAQAN ASADASSAVM SETGCRHYQS
260 270 280 290 300
YVKEHSYDTF RVIDAYFAAC VNRDARERKA IHCNCFECGS YGIQLYACLH
310 320 330 340 350
CIYFGCRGAH ITSHLRSKKH NVALELSHGT LYCYACRDFI YDARSREYAL
360 370 380 390 400
INRKLEAKDL QKSIGWVPWV PTTKETNLLL ANARRRLVRP NQTIGLRGLL
410 420 430 440 450
NLGATCFMNC IVQALVHTPL LSDYFMSDRH DCGSKSSHKC LVCEVSRLFQ
460 470 480 490 500
EFYSGSRSPL SLHRLLHLIW NHAKHLAGYE QQDAHEFFIA TLDVLHRHCV
510 520 530 540 550
KAKAEHESKS NSSGSGSGTN SSNSSSSHCY GQCNCIIDQI FTGMLQSDVV
560 570 580 590 600
CQACNGVSTT YDPFWDISLD LGETTTHGGV TPKTLIDCLE RYTRAEHLGS
610 620 630 640 650
AAKIKCSTCK SYQESTKQFS LRTLPSVVSF HLKRFEHSAL IDRKISSFIQ
660 670 680 690 700
FPVEFDMTPF MSEKKNAYGD FRFSLYAVVN HVGTIDTGHY TAYVRHQKDT
710 720 730
WVKCDDHVIT MASLKQVLDS EGYLLFYHKN VLEYE
Length:735
Mass (Da):82,458
Last modified:April 14, 2009 - v3
Checksum:iEAE3F4B1DBFF850F
GO

Sequence cautioni

The sequence AAL13936.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAF49249.2 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901A → T in AAD53181. (PubMed:11182084)Curated
Sequence conflicti199 – 1991H → N in AAD53181. (PubMed:11182084)Curated
Sequence conflicti235 – 2351A → D in AAD53181. (PubMed:11182084)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF179590 Genomic DNA. Translation: AAD53181.1.
AE014296 Genomic DNA. Translation: AAF49249.2. Sequence problems.
AY058707 mRNA. Translation: AAL13936.1. Different initiation.
RefSeqiNP_524140.2. NM_079416.4.
UniGeneiDm.2731.

Genome annotation databases

GeneIDi40030.
KEGGidme:Dmel_CG4166.
UCSCiCG4166-RB. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF179590 Genomic DNA. Translation: AAD53181.1 .
AE014296 Genomic DNA. Translation: AAF49249.2 . Sequence problems.
AY058707 mRNA. Translation: AAL13936.1 . Different initiation.
RefSeqi NP_524140.2. NM_079416.4.
UniGenei Dm.2731.

3D structure databases

ProteinModelPortali Q9VVR1.
SMRi Q9VVR1. Positions 296-727.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 65311. 5 interactions.
IntActi Q9VVR1. 4 interactions.
MINTi MINT-6179419.
STRINGi 7227.FBpp0288698.

Protein family/group databases

MEROPSi C19.095.

Proteomic databases

PaxDbi Q9VVR1.
PRIDEi Q9VVR1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 40030.
KEGGi dme:Dmel_CG4166.
UCSCi CG4166-RB. d. melanogaster.

Organism-specific databases

CTDi 40030.
FlyBasei FBgn0013717. not.

Phylogenomic databases

eggNOGi COG5533.
InParanoidi Q9VVR1.
KOi K11366.
OrthoDBi EOG7FR7G7.
PhylomeDBi Q9VVR1.

Miscellaneous databases

GenomeRNAii 40030.
NextBioi 816633.

Gene expression databases

Bgeei Q9VVR1.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR015880. Znf_C2H2-like.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glial cells mediate target layer selection of retinal axons in the developing visual system of Drosophila."
    Poeck B., Fischer S., Gunning D., Zipursky S.L., Salecker I.
    Neuron 29:99-113(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 27-735.
    Strain: Berkeley.
    Tissue: Embryo.
  5. "SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system."
    Weake V.M., Lee K.K., Guelman S., Lin C.-H., Seidel C., Abmayr S.M., Workman J.L.
    EMBO J. 27:394-405(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, IDENTIFICATION IN THE SAGA COMPLEX, INTERACTION WITH SGF11.
  6. "A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing."
    Zhao Y., Lang G., Ito S., Bonnet J., Metzger E., Sawatsubashi S., Suzuki E., Le Guezennec X., Stunnenberg H.G., Krasnov A., Georgieva S.G., Schuele R., Takeyama K., Kato S., Tora L., Devys D.
    Mol. Cell 29:92-101(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNOT_DROME
AccessioniPrimary (citable) accession number: Q9VVR1
Secondary accession number(s): Q95TK9, Q9U6Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: October 29, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3