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Protein

Ubiquitin carboxyl-terminal hydrolase nonstop

Gene

not

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of histone H2B, thereby acting as a coactivator in a large subset of genes. Required to counteract heterochromatin silencing. Controls the development of neuronal connectivity in visual system by being required for accurate axon targeting in the optic lobe. Required for expression of ecdysone-induced genes such as br/broad.3 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167NucleophilePROSITE-ProRule annotation1
Active sitei450Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 103UBP-typePROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: FlyBase
  • axon target recognition Source: FlyBase
  • chromatin organization Source: UniProtKB
  • glial cell development Source: FlyBase
  • glial cell migration Source: FlyBase
  • histone deubiquitination Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • protein deubiquitination Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process Source: FlyBase

Keywordsi

Molecular functionActivator, Chromatin regulator, Hydrolase, Protease, Thiol protease
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.095.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase nonstop (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme nonstop
Ubiquitin thioesterase nonstop
Ubiquitin-specific-processing protease nonstop
Gene namesi
Name:not
ORF Names:CG4166
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0013717. not.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in the number and migration of glial cells located within the lamina plexus of the developing eye; the lack of glial cells causing mistargeting of the R1-R6 axons in the optic lobe. Lamina neuron development is normal.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003675141 – 496Ubiquitin carboxyl-terminal hydrolase nonstopAdd BLAST496

Proteomic databases

PaxDbiQ9VVR1.
PRIDEiQ9VVR1.

Expressioni

Tissue specificityi

Expressed in the optic lobe and central brain. Highly expressed in the lamina precursor cells but not in differentiated lamina neurons. Also expressed in marginal, epithelial and medulla glial cells adjacent to the lamina plexus.1 Publication

Gene expression databases

BgeeiFBgn0013717.
ExpressionAtlasiQ9VVR1. differential.
GenevisibleiQ9VVR1. DM.

Interactioni

Subunit structurei

Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.1 Publication

Protein-protein interaction databases

BioGridi65311. 15 interactors.
IntActiQ9VVR1. 4 interactors.
MINTiMINT-6179419.
STRINGi7227.FBpp0288698.

Structurei

3D structure databases

ProteinModelPortaliQ9VVR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 491USPPROSITE-ProRule annotationAdd BLAST334

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 103UBP-typePROSITE-ProRule annotationAdd BLAST60

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
GeneTreeiENSGT00890000139343.
InParanoidiQ9VVR1.
KOiK11366.
OrthoDBiEOG091G09GI.
PhylomeDBiQ9VVR1.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VVR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETGCRHYQ SYVKEHSYDT FRVIDAYFAA CVNRDARERK AIHCNCFECG
60 70 80 90 100
SYGIQLYACL HCIYFGCRGA HITSHLRSKK HNVALELSHG TLYCYACRDF
110 120 130 140 150
IYDARSREYA LINRKLEAKD LQKSIGWVPW VPTTKETNLL LANARRRLVR
160 170 180 190 200
PNQTIGLRGL LNLGATCFMN CIVQALVHTP LLSDYFMSDR HDCGSKSSHK
210 220 230 240 250
CLVCEVSRLF QEFYSGSRSP LSLHRLLHLI WNHAKHLAGY EQQDAHEFFI
260 270 280 290 300
ATLDVLHRHC VKAKAEHESK SNSSGSGSGT NSSNSSSSHC YGQCNCIIDQ
310 320 330 340 350
IFTGMLQSDV VCQACNGVST TYDPFWDISL DLGETTTHGG VTPKTLIDCL
360 370 380 390 400
ERYTRAEHLG SAAKIKCSTC KSYQESTKQF SLRTLPSVVS FHLKRFEHSA
410 420 430 440 450
LIDRKISSFI QFPVEFDMTP FMSEKKNAYG DFRFSLYAVV NHVGTIDTGH
460 470 480 490
YTAYVRHQKD TWVKCDDHVI TMASLKQVLD SEGYLLFYHK NVLEYE
Length:496
Mass (Da):56,441
Last modified:August 30, 2017 - v4
Checksum:i9EB4690FB968A3DE
GO

Sequence cautioni

Q9VVR1: The sequence AAD53181 differs from that shown. Reason: Erroneous gene model prediction.Curated
Q9VVR1: The sequence AAL13936 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179590 Genomic DNA. Translation: AAD53181.1. Sequence problems.
AE014296 Genomic DNA. Translation: AAF49249.2.
AE014296 Genomic DNA. Translation: AHN58131.1.
AY058707 mRNA. Translation: AAL13936.1. Different initiation.
RefSeqiNP_001287106.1. NM_001300177.1.
NP_524140.2. NM_079416.4.
UniGeneiDm.2731.

Genome annotation databases

EnsemblMetazoaiFBtr0290259; FBpp0288698; FBgn0013717.
FBtr0346438; FBpp0312097; FBgn0013717.
GeneIDi40030.
KEGGidme:Dmel_CG4166.
UCSCiCG4166-RB. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiNOT_DROME
AccessioniPrimary (citable) accession number: Q9VVR1
Secondary accession number(s): Q95TK9, Q9U6Q9, X2JCK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: August 30, 2017
Last modified: October 25, 2017
This is version 143 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families