Reviewed, UniProtKB/Swiss-Prot Q9VVL6 (MED19_DROME)

Names and origin

Protein names

Recommended name:
    Mediator of RNA polymerase II transcription subunit 19
Alternative name(s):
    Mediator complex subunit 19

Gene names

Name:	MED19
ORF Names:	CG5546

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.
Subunit structure	Component of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 19 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mediator complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA polymerase II transcription mediator activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8SYU5	1	EBI-125442,EBI-116042
	Q9VRX7	1	EBI-125442,EBI-108965
ple	P18459-2	1	EBI-125442,EBI-198251
Srp68	Q9VSS2	1	EBI-125442,EBI-189424

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Mediator of RNA polymerase II transcription subunit 19		PRO_0000304773
Regions
	Coiled coil	189 – 223	35	Potential
	Compositional bias	227 – 330	104	Gly-rich
	Compositional bias	284 – 297	14	Gln-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9VVL6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 46407EBF573EC80D	FASTA	337	35,390
	10 20 30 40 50 60 MMSNYGNMMD SPKSSPHGGG RSPVVARQDS SGTLKTTISL GKTPTIIHTG PFYSMKEPPA 70 80 90 100 110 120 KAELTGDKDL MTEYGLHHTL TKFKEKKFKE SLASFLQNIP GINDLITHPV ENSTLRSVIE 130 140 150 160 170 180 KPPIGGKELL PLTPVQLAGF RLHPGPLPEQ YRTTYVTPAR KHKNKHKKQK HKDGVTTGQE 190 200 210 220 230 240 STLLDSAGLE TYEKKHKKQK RHEDDKERKK RKKEKKRKKK NQSPEPGVGL LPGGASLGGA 250 260 270 280 290 300 AGVMGATSLG SLGGGPGGPG LSSLSASMGP GVVGNMNSFS SSMQMQQQQQ MPMQQQQMSS 310 320 330 GGLLGSVLGT SGGPGGGGGG GGGGGGGVGG SLLMSQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF49295.1. AY069647 mRNA. Translation: AAL39792.1.
RefSeq	NP_649016.1.
UniGene	Dm.2027
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VVL6. 22 interactions.
Proteomic databases
PRIDE	Q9VVL6.
Genome annotation databases
Ensembl	FBgn0036761. Drosophila melanogaster. [Contig view]
GeneID	39987.
KEGG	dme:Dmel_CG5546.
NMPDR	fig|7227.3.peg.10376.
Organism-specific databases
FlyBase	FBgn0036761. MED19.
Phylogenomic databases
HOGENOM	Q9VVL6.
OMA	Q9VVL6. ITHPVEN.
Gene expression databases
ArrayExpress	Q9VVL6.
Family and domain databases
InterPro	IPR019403. Mediator_Med19_met. [Graphical view]
Pfam	PF10278. Med19. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816420.

Entry information

Entry name

MED19_DROME

Accession

Primary (citable) accession number: Q9VVL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project