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Protein

Enhancer of mRNA-decapping protein 3

Gene

Edc3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In the process of mRNA degradation, may play a role in mRNA decapping.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-430039. mRNA decay by 5' to 3' exoribonuclease.

Names & Taxonomyi

Protein namesi
Recommended name:
Enhancer of mRNA-decapping protein 3
Gene namesi
Name:Edc3
ORF Names:CG6311
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0036735. Edc3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: UniProtKB
  • microtubule associated complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25Q → A: Strongly reduced interaction with DCP1A. No effect on P-body targeting. 1 Publication1
Mutagenesisi42F → A: Strongly reduced interaction with DCP1A, but no effect on P-body targeting; when associated with A-44. 1 Publication1
Mutagenesisi44N → A: Strongly reduced interaction with DCP1A, but no effect on P-body targeting; when associated with A-42. 1 Publication1
Mutagenesisi345F → A: Strongly reduced interaction with DDX6. 1 Publication1
Mutagenesisi347F → A: Minor effect on interaction with DDX6. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003307301 – 680Enhancer of mRNA-decapping protein 3Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136Phosphoserine1 Publication1
Modified residuei188Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VVI2.
PRIDEiQ9VVI2.

PTM databases

iPTMnetiQ9VVI2.

Expressioni

Gene expression databases

BgeeiFBgn0036735.
ExpressionAtlasiQ9VVI2. baseline.
GenevisibleiQ9VVI2. DM.

Interactioni

Subunit structurei

Forms a complex with DCP1A, DCP2, Me31B/DDX6 and EDC4/HEDLS (By similarity). Within this complex directly interacts with DCP1A, DCP2 and Me31B/DDX6. Homooligomer.By similarity3 Publications

Protein-protein interaction databases

BioGridi65245. 2 interactors.
IntActiQ9VVI2. 3 interactors.
MINTiMINT-903966.
STRINGi7227.FBpp0074970.

Structurei

Secondary structure

1680
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi12 – 17Combined sources6
Turni19 – 21Combined sources3
Beta strandi23 – 32Combined sources10
Beta strandi35 – 43Combined sources9
Beta strandi51 – 53Combined sources3
Beta strandi55 – 58Combined sources4
Turni59 – 61Combined sources3
Beta strandi62 – 69Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RM4NMR-A1-101[»]
ProteinModelPortaliQ9VVI2.
SMRiQ9VVI2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9VVI2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini333 – 369DFDFPROSITE-ProRule annotationAdd BLAST37
Domaini430 – 658YjeF N-terminalPROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 101Required for P-body targeting and interaction with DCP1A1 PublicationAdd BLAST101
Regioni102 – 338Required for interaction with DCP21 PublicationAdd BLAST237
Regioni331 – 440Required for interaction with Me31BAdd BLAST110

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi103 – 132Gln-richAdd BLAST30
Compositional biasi278 – 315Asn/Gly-richAdd BLAST38

Sequence similaritiesi

Belongs to the EDC3 family.Curated
Contains 1 DFDF domain.PROSITE-ProRule annotation
Contains 1 YjeF N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000016435.
InParanoidiQ9VVI2.
KOiK12615.
OMAiPANNHQW.
OrthoDBiEOG091G09C2.
PhylomeDBiQ9VVI2.

Family and domain databases

InterProiIPR025762. DFDF.
IPR019050. FDF_dom.
IPR025609. Lsm14_N.
IPR004443. YjeF_N_dom.
[Graphical view]
SMARTiSM01199. FDF. 1 hit.
SM01271. LSM14. 1 hit.
[Graphical view]
PROSITEiPS51512. DFDF. 1 hit.
PS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VVI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPTDQDWIG CAVSIACDEV LGVFQGLIKQ ISAEEITIVR AFRNGVPLRK
60 70 80 90 100
QNAEVVLKCT DIRSIDLIEP AKQDLDGHTA PPPVVNKPTP VKLPHFSNIL
110 120 130 140 150
GKQQQLQLQQ QQQQLQLQQQ QKQQFQEQER EQDLPSTPRS RANDNGRVAA
160 170 180 190 200
GGASSSSGPR GNFNSALSDK MHQLKLIETN GSNGTLRTPQ TSRASSAQQQ
210 220 230 240 250
PQISTTPNSV AAFFGNMIPP KVEVKLGSYV SNTRESYCSS SGDSGEATGL
260 270 280 290 300
SLGSSKPIDI VSNGDGFYKQ TAASSYGNTN GNVRRNGNAN NNGNGTGNGS
310 320 330 340 350
YTNGNGNGNG KNKRNRVRRE SSMRQQQVQL TFGSEADDPL IHEDFDFEGN
360 370 380 390 400
LALFDKQAIW DDIESTTQKP DVVRHIVNNH HHKPEQKYRH DENILASKPL
410 420 430 440 450
QLRQIESMFG GSQDFVTDDG LIIPTIPAYV RNKIEISADK AGLSLQRQID
460 470 480 490 500
ILARGASDLA ITLLGGARRL TPANNHQWPK IAIICDGGKN MRTINIGAAT
510 520 530 540 550
GRQLASHGLT VLLYVEQAKL LEQNSSSPEI SLFKATDNVI VHSVDALPTP
560 570 580 590 600
DLVILSTNTA NLSDAIRKWL SVNRASVLAI DPPPCGINEV AIKYSILPIL
610 620 630 640 650
PLNGISTATT SSSSSAAATP TPIASTSAAA SATKSAASTN NCGKLYLCNL
660 670 680
GIPDKFYRDC GIKYKSPYGH KYVIPIHSKD
Length:680
Mass (Da):73,415
Last modified:May 1, 2000 - v1
Checksum:i10B567618DF26CB6
GO

Sequence cautioni

The sequence AAL25455 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL39717 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49329.1.
AY069572 mRNA. Translation: AAL39717.1. Different initiation.
AY060416 mRNA. Translation: AAL25455.1. Different initiation.
RefSeqiNP_648992.1. NM_140735.3.
UniGeneiDm.2069.

Genome annotation databases

EnsemblMetazoaiFBtr0075208; FBpp0074970; FBgn0036735.
GeneIDi39957.
KEGGidme:Dmel_CG6311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49329.1.
AY069572 mRNA. Translation: AAL39717.1. Different initiation.
AY060416 mRNA. Translation: AAL25455.1. Different initiation.
RefSeqiNP_648992.1. NM_140735.3.
UniGeneiDm.2069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RM4NMR-A1-101[»]
ProteinModelPortaliQ9VVI2.
SMRiQ9VVI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65245. 2 interactors.
IntActiQ9VVI2. 3 interactors.
MINTiMINT-903966.
STRINGi7227.FBpp0074970.

PTM databases

iPTMnetiQ9VVI2.

Proteomic databases

PaxDbiQ9VVI2.
PRIDEiQ9VVI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075208; FBpp0074970; FBgn0036735.
GeneIDi39957.
KEGGidme:Dmel_CG6311.

Organism-specific databases

CTDi80153.
FlyBaseiFBgn0036735. Edc3.

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000016435.
InParanoidiQ9VVI2.
KOiK12615.
OMAiPANNHQW.
OrthoDBiEOG091G09C2.
PhylomeDBiQ9VVI2.

Enzyme and pathway databases

ReactomeiR-DME-430039. mRNA decay by 5' to 3' exoribonuclease.

Miscellaneous databases

EvolutionaryTraceiQ9VVI2.
GenomeRNAii39957.
PROiQ9VVI2.

Gene expression databases

BgeeiFBgn0036735.
ExpressionAtlasiQ9VVI2. baseline.
GenevisibleiQ9VVI2. DM.

Family and domain databases

InterProiIPR025762. DFDF.
IPR019050. FDF_dom.
IPR025609. Lsm14_N.
IPR004443. YjeF_N_dom.
[Graphical view]
SMARTiSM01199. FDF. 1 hit.
SM01271. LSM14. 1 hit.
[Graphical view]
PROSITEiPS51512. DFDF. 1 hit.
PS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEDC3_DROME
AccessioniPrimary (citable) accession number: Q9VVI2
Secondary accession number(s): Q8T042, Q95SZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.