Reviewed, UniProtKB/Swiss-Prot Q9VVF3 (OR74A_DROME)

Names and origin

Protein names

Recommended name:
    Putative odorant receptor 74a

Gene names

Name:	Or74a
ORF Names:	CG13726

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Or83b. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW detection of chemical stimulus involved in sensory perception of smell Inferred from mutant phenotype. Source: FlyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VDT2	1	EBI-170685,EBI-86445

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Putative odorant receptor 74a		PRO_0000174269
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	1 Potential
	Topological domain	60 – 67	8	Extracellular Potential
	Transmembrane	68 – 88	21	2 Potential
	Topological domain	89 – 141	53	Cytoplasmic Potential
	Transmembrane	142 – 162	21	3 Potential
	Topological domain	163 – 181	19	Extracellular Potential
	Transmembrane	182 – 202	21	4 Potential
	Topological domain	203 – 274	72	Cytoplasmic Potential
	Transmembrane	275 – 295	21	5 Potential
	Topological domain	296 – 303	8	Extracellular Potential
	Transmembrane	304 – 324	21	6 Potential
	Topological domain	325 – 380	56	Cytoplasmic Potential
	Transmembrane	381 – 401	21	7 Potential
	Topological domain	402 – 404	3	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VVF3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C61127EB0D422206	FASTA	404	47,415
	10 20 30 40 50 60 MSFHRYRPRL PGGELAPMPW PVSLYRVLNH VAWPLEAESG RWTVFLDRLM IFLGFLVFCE 70 80 90 100 110 120 HNEVDFHYLI ANRQDMDNML TGLPTYLILV EMQIRCFQLA WHKDRFRALL QRFYAEIYVS 130 140 150 160 170 180 EEMEPHLFAS IQRQMLATRV NSTVYLLALL NFFLVPVTNV IYHRREMLYK QVYPFDNTQL 190 200 210 220 230 240 HFFIPLLVLN FWVGFIITSM LFGELNVMGE LMMHLNARYI QLGQDLRRSA QMLLKKSSSL 250 260 270 280 290 300 NVAIAYRLNL THILRRNAAL RDFGQRVEKE FTLRIFVMFA FSAGLLCALF FKAFTNPWGN 310 320 330 340 350 360 VAYIVWFLAK FMELLALGML GSILLKTTDE LGMMYYTADW EQVIHQSDNV GENVKLMKLV 370 380 390 400 TLAIQLNSRP FFITGLNYFR VSLTAVLKII QGAFSYFTFL NSMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF49358.1.
RefSeq	NP_524123.1.
UniGene	Dm.27162
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:21424N.
IntAct	Q9VVF3. 1 interaction.
Proteomic databases
PRIDE	Q9VVF3.
Genome annotation databases
Ensembl	FBgn0036709. Drosophila melanogaster. [Contig view]
GeneID	39929.
KEGG	dme:Dmel_CG13726.
NMPDR	fig|7227.3.peg.10293.
Organism-specific databases
FlyBase	FBgn0036709. Or74a.
Phylogenomic databases
HOGENOM	Q9VVF3.
OMA	Q9VVF3. YLILVEM.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-017098-MON.
Gene expression databases
ArrayExpress	Q9VVF3.
GermOnline	CG13726. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816127.

Entry information

Entry name

OR74A_DROME

Accession

Primary (citable) accession number: Q9VVF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project