Q9VVE5 (MSIR6_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein Musashi homolog Rbp6

Gene names

Name:	Rbp6
Synonyms:	RRM6
ORF Names:	CG32169

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. UniProtKB Q920Q6
Subcellular location	Cytoplasm By similarity UniProtKB Q920Q6.
Sequence similarities	Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	stem cell development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB polysome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro poly(U) RNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A Ref.1 (identifier: Q9VVE5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B Ref.1 (identifier: Q9VVE5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-369: DNIMWGLGTL...YSLNFAMANI → GELVVWGSSH...AASLYQLPGI

Isoform C Ref.1 (identifier: Q9VVE5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-112: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	RNA-binding protein Musashi homolog Rbp6		PRO_0000374051
Regions
	Domain	29 – 108	80	RRM 1
	Domain	118 – 195	78	RRM 2
Natural variations
	Alternative sequence	1 – 112	112	Missing in isoform C. Ref.1		VSP_053086
	Alternative sequence	216 – 369	154	DNIMW…AMANI → GELVVWGSSHAHSTAATSAA AAGLLPSSLAAAASVLQQQQ QQQQQQQQQQQQQQQHHQQL HHTHPQSPAHSHAHHPHTHS HSQLLGSLRYTPYPLPAHLS AAAVVVAQQQHHHQQQQQQQ QQQQQQQVVAAQQQHQQQQS LAQVVAAAAGAAPGLLPLAN PAPPATPSLLQFAAGQSNAA LANSLYADAAAVVGYKRLLA AAAVSSGLRAPTTALGALQA AAANVPAAAAAAQLQQAQLR QNAALAAAHYPLSELLAMQG GMEMGAGANSAAAAAASLYQ LPGI in isoform B. Ref.1		VSP_053087
Experimental info
	Sequence conflict	33	1	I → V in AAM52738. Ref.4
	Sequence conflict	73	1	G → A in AAM52738. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 26, 2005. Version 3. Checksum: ADDA310C4CF993C4	FASTA	369	40,087
	10 20 30 40 50 60 MDIKIEQQQQ QQQVELGPCS PSEVPNDPGK MFIGGLSWQT SPESLRDYFG RYGDISEAMV 70 80 90 100 110 120 MKDPTTRRSR GFGFVTFSDP NSVDKVLTQG THELDGKKVD PKVAFPRRAH PKMVTRTKKI 130 140 150 160 170 180 FVGGLSAPTT LEDVKSYFEQ FGPIEDAMLM FDKQTNRHRG FGFVTFQSED VVDKVCEIHF 190 200 210 220 230 240 HEINNKMVEC KKAQPKEVML PANLAKTRAA GRSAYDNIMW GLGTLPEGFP AAAYAAYAAG 250 260 270 280 290 300 RGYSGYPSFG LPYPTVDLTN GQLTPNNNTP LLTQALSVMN NYQAAAAAAH SYGPSASPHA 310 320 330 340 350 360 AAANTATRQG FPSTNSPGPT IDMYSSAAAD NVGYVQATSP QPSGFPIAVS RAPLNYSPIY SLNFAMANI
	Isoform B [UniParc]. Checksum: 9C72B16A989F8342	FASTA	499	53,487
	10 20 30 40 50 60 MDIKIEQQQQ QQQVELGPCS PSEVPNDPGK MFIGGLSWQT SPESLRDYFG RYGDISEAMV 70 80 90 100 110 120 MKDPTTRRSR GFGFVTFSDP NSVDKVLTQG THELDGKKVD PKVAFPRRAH PKMVTRTKKI 130 140 150 160 170 180 FVGGLSAPTT LEDVKSYFEQ FGPIEDAMLM FDKQTNRHRG FGFVTFQSED VVDKVCEIHF 190 200 210 220 230 240 HEINNKMVEC KKAQPKEVML PANLAKTRAA GRSAYGELVV WGSSHAHSTA ATSAAAAGLL 250 260 270 280 290 300 PSSLAAAASV LQQQQQQQQQ QQQQQQQQQQ HHQQLHHTHP QSPAHSHAHH PHTHSHSQLL 310 320 330 340 350 360 GSLRYTPYPL PAHLSAAAVV VAQQQHHHQQ QQQQQQQQQQ QQVVAAQQQH QQQQSLAQVV 370 380 390 400 410 420 AAAAGAAPGL LPLANPAPPA TPSLLQFAAG QSNAALANSL YADAAAVVGY KRLLAAAAVS 430 440 450 460 470 480 SGLRAPTTAL GALQAAAANV PAAAAAAQLQ QAQLRQNAAL AAAHYPLSEL LAMQGGMEMG 490 AGANSAAAAA ASLYQLPGI
	Isoform C [UniParc]. Checksum: 4AFA020CE3404168	FASTA	257	27,522
	10 20 30 40 50 60 MVTRTKKIFV GGLSAPTTLE DVKSYFEQFG PIEDAMLMFD KQTNRHRGFG FVTFQSEDVV 70 80 90 100 110 120 DKVCEIHFHE INNKMVECKK AQPKEVMLPA NLAKTRAAGR SAYDNIMWGL GTLPEGFPAA 130 140 150 160 170 180 AYAAYAAGRG YSGYPSFGLP YPTVDLTNGQ LTPNNNTPLL TQALSVMNNY QAAAAAAHSY 190 200 210 220 230 240 GPSASPHAAA ANTATRQGFP STNSPGPTID MYSSAAADNV GYVQATSPQP SGFPIAVSRA 250 PLNYSPIYSL NFAMANI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[4]	"Isolation of RRM-type RNA-binding protein genes and the analysis of their relatedness by using a numerical approach." Kim Y.-J., Baker B.S. Mol. Cell. Biol. 13:174-183(1993) [PubMed: 8417324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-75 (ISOFORMS A/B).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014296 Genomic DNA. Translation: AAF49366.3. AE014296 Genomic DNA. Translation: ABW08559.1. AE014296 Genomic DNA. Translation: ABW08560.1. AY122226 mRNA. Translation: AAM52738.1. S51715 mRNA. Translation: AAB24627.1.
PIR	F48110.
RefSeq	NP_001097631.1. NM_001104161.1. NP_001097632.1. NM_001104162.2. NP_730245.2. NM_168718.2.
UniGene	Dm.22292.
3D structure databases
HSSP	HSSP built from PDB template 2MST based on UniProtKB Q61474.
ProteinModelPortal	Q9VVE5.
SMR	Q9VVE5. Positions 27-197.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19056N.
IntAct	Q9VVE5. 24 interactions.
MINT	MINT-918255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075285; FBpp0075045; FBgn0260943.
GeneID	39919.
KEGG	dme:Dmel_CG32169.
UCSC	CG32169-RA. d. melanogaster.
Organism-specific databases
CTD	39919.
FlyBase	FBgn0260943. Rbp6.
Phylogenomic databases
eggNOG	inNOG07954.
GeneTree	EMGT00050000000075.
InParanoid	Q9VVE5.
OMA	SAYDNIM.
OrthoDB	EOG4MSBF6.
PhylomeDB	Q9VVE5.
Gene expression databases
Bgee	Q9VVE5.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
KO	K14411.
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	816085.

Entry information

Entry name

MSIR6_DROME

Accession

Primary (citable) accession number: Q9VVE5
Secondary accession number(s): A8JNU7 , A8JNU8, Q26276, Q8MQZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project