Q9VV96 (PGSB2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidoglycan-recognition protein SB2
EC=3.5.1.28

Gene names

Name:	PGRP-SB2
ORF Names:	CG9697

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Cofactor	Zinc By similarity.
Subcellular location	Secreted Potential.
Developmental stage	Not expressed in adults. Ref.4
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	innate immune response Non-traceable author statement Ref.4. Source: UniProtKB peptidoglycan catabolic process Non-traceable author statement Ref.4. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC peptidoglycan binding Non-traceable author statement Ref.4. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 182	165	Peptidoglycan-recognition protein SB2		PRO_0000023908
Sites
	Metal binding	48	1	Zinc By similarity
	Metal binding	81	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	163	1	Zinc By similarity
Amino acid modifications
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 60		By similarity
Natural variations
	Natural variant	15	1	A → V in strain: ZW141.
	Natural variant	51	1	T → A in strain: Loua.
	Natural variant	62	1	L → S in strain: ZW141.
	Natural variant	152	1	V → L in strain: Draveil, Loua, Monty5, P.bourg, Tahiti, Texas and ZW141.

Sequences

	Sequence		Length	Mass (Da)
	Q9VV96 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 99AC18AD426BC308	FASTA	182	20,459
	10 20 30 40 50 60 MKLQLALVLC GLTLALGQIV PRSSWCPVPI SPRMPRLMVP VRLIIIHHTV TAPCFNPHQC 70 80 90 100 110 120 QLVLRQIRAD HMRRKFRDIG YNFLIGGDGR IYEGLGFGIR GEHAPRYNSQ SIGIAFIGNF 130 140 150 160 170 180 QTGLPPSQML QAARTLIQIA VQRRQVSPNY SVVGHCQTKA TACPGIHLLN ELKKWPNWRP KP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ556575 Genomic DNA. Translation: CAD89140.1. AJ556576 Genomic DNA. Translation: CAD89141.1. AJ556577 Genomic DNA. Translation: CAD89142.1. AJ556578 Genomic DNA. Translation: CAD89143.1. AJ556579 Genomic DNA. Translation: CAD89144.1. AJ556580 Genomic DNA. Translation: CAD89145.1. AJ556581 Genomic DNA. Translation: CAD89146.1. AJ556582 Genomic DNA. Translation: CAD89147.1. AJ556583 Genomic DNA. Translation: CAD89148.1. AJ556584 Genomic DNA. Translation: CAD89149.1. AJ556585 Genomic DNA. Translation: CAD89150.1. AE014296 Genomic DNA. Translation: AAF49421.1.
RefSeq	NP_648916.1. NM_140659.3.
3D structure databases
ProteinModelPortal	Q9VV96.
SMR	Q9VV96. Positions 40-182.
ModBase	Search...
Proteomic databases
PRIDE	Q9VV96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075320; FBpp0075079; FBgn0043577.
GeneID	39869.
KEGG	dme:Dmel_CG9697.
UCSC	CG9697-RA. d. melanogaster.
Organism-specific databases
CTD	39869.
FlyBase	FBgn0043577. PGRP-SB2.
Phylogenomic databases
eggNOG	NOG241935.
GeneTree	ENSGT00390000016833.
InParanoid	Q9VV96.
KO	K01446.
OMA	YNSQSIG.
OrthoDB	EOG4NVX2K.
PhylomeDB	Q9VV96.
Gene expression databases
Bgee	Q9VV96.
GermOnline	CG9697. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.80.10. 1 hit.
InterPro	IPR002502. Amidase_domain. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_domain_met/bac. [Graphical view]
PANTHER	PTHR11022. PTHR11022. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. [Graphical view]
PIRSF	PIRSF037945. PGRPs. 1 hit.
SMART	SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view]
SUPFAM	SSF55846. Amidase_2. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	39869.
NextBio	815800.

Entry information

Entry name

PGSB2_DROME

Accession

Primary (citable) accession number: Q9VV96
Secondary accession number(s): Q70PX1, Q70PX3, Q70PX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project