Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase mind-bomb

Gene

mib1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta (Dl) and Serrate (Ser) receptors, which act as ligands of Notch proteins. Positively regulates the Notch signaling by ubiquitinating the intracellular domain of Dl and Ser, leading to endocytosis of Dl and Ser receptors. Regulates a subset of Notch signaling events, including wing margin specification, leg segmentation and vein determination, that are distinct from those events requiring neuralize (neur) activity. Also modulates lateral inhibition, a neur- and Dl-dependent signaling event, suggesting a distinct but partially complementary function with neur.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri173 – 220ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri970 – 1005RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri1017 – 1052RING-type 2PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri1183 – 1216RING-type 3PROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB
  • lateral inhibition Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • positive regulation of Notch signaling pathway Source: UniProtKB
  • protein localization Source: UniProtKB
  • protein polyubiquitination Source: FlyBase
  • protein ubiquitination Source: UniProtKB
  • regulation of Notch signaling pathway Source: UniProtKB
  • sensory organ development Source: FlyBase

Keywordsi

Molecular functionTransferase
Biological processNotch signaling pathway, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ9VUX2.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase mind-bomb (EC:2.3.2.27)
Alternative name(s):
Mind bomb homolog
Short name:
D-mib
RING-type E3 ubiquitin transferase mind-bombCurated
Gene namesi
Name:mib1
Synonyms:mind-bomb
ORF Names:CG5841
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0263601. mib1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi457V → M in D-mib4; induces defects in Notch signaling. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559511 – 1226E3 ubiquitin-protein ligase mind-bombAdd BLAST1226

Proteomic databases

PaxDbiQ9VUX2.
PRIDEiQ9VUX2.

Expressioni

Tissue specificityi

Ubiquitous in the wing imaginal disk (at protein level).2 Publications

Gene expression databases

BgeeiFBgn0263601.
ExpressionAtlasiQ9VUX2. differential.
GenevisibleiQ9VUX2. DM.

Interactioni

Subunit structurei

Interacts with intracellular domain of Dl and Ser.1 Publication

Protein-protein interaction databases

BioGridi65061. 7 interactors.
IntActiQ9VUX2. 1 interactor.
MINTiMINT-865060.
STRINGi7227.FBpp0075275.

Structurei

3D structure databases

ProteinModelPortaliQ9VUX2.
SMRiQ9VUX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 168MIB/HERC2 1PROSITE-ProRule annotationAdd BLAST69
Domaini237 – 315MIB/HERC2 2PROSITE-ProRule annotationAdd BLAST79
Repeati567 – 596ANK 1Add BLAST30
Repeati600 – 629ANK 2Add BLAST30
Repeati633 – 662ANK 3Add BLAST30
Repeati666 – 695ANK 4Add BLAST30
Repeati699 – 731ANK 5Add BLAST33
Repeati735 – 765ANK 6Add BLAST31
Repeati769 – 798ANK 7Add BLAST30
Repeati802 – 833ANK 8Add BLAST32

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1159 – 1181Sequence analysisAdd BLAST23

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri173 – 220ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri970 – 1005RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri1017 – 1052RING-type 2PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri1183 – 1216RING-type 3PROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4582. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00900000141005.
InParanoidiQ9VUX2.
KOiK10645.
OMAiCYNERKT.
OrthoDBiEOG091G00Z0.
PhylomeDBiQ9VUX2.

Family and domain databases

CDDicd00204. ANK. 2 hits.
Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR010606. Mib_Herc2.
IPR037252. Mib_Herc2_sf.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
PRINTSiPR01415. ANKYRIN.
SMARTiView protein in SMART
SM00248. ANK. 8 hits.
SM00184. RING. 3 hits.
SM00291. ZnF_ZZ. 1 hit.
SUPFAMiSSF159034. SSF159034. 2 hits.
SSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 3 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VUX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCAATLSSA KDSTNANASG GGGGGGGGGA PTNSNTNTNT NTQSTAVGVV
60 70 80 90 100
VSSAAGTGVG VGGGGGGGGS LPGGTTSSSS ASAAGGVAAG GGGNSAAALV
110 120 130 140 150
RRFSMEGVGA RVIRGPDWKW NKQDGGEGHV GTVRNFESAE EVVVVWDNGT
160 170 180 190 200
AANYRCAGAY DLRILDSAPT GVKHEGTMCD TCRQQPIFGI RWKCAECINY
210 220 230 240 250
DLCSICYHGD KHHLRHRFYR ITTPGGERTM LEPRRKSKKV LARGIFPGAR
260 270 280 290 300
VVRGVDWQWE DQDGGVGRRG KVNEIQDWSS ASPRSAAYVI WDNGSKNLYR
310 320 330 340 350
VGFEGMADLK VVNDAKGSNV YRDHLPLLGE NGPGKGPHGF QIGDKVTVDL
360 370 380 390 400
DLEIVQSLQH GHGGWTDGMF ECLSNAGMVV GIDEDHDIVV AYNSGNRWTF
410 420 430 440 450
NPAVLTKVSS PTTAPPEFQV GDIVKICSDV ESIKILQRGH GEWADAMQLT
460 470 480 490 500
LGKIGRVQQV YHDNDLKVEV GNTSWTYNPL AVCKVASSTA SDGSCAPVIP
510 520 530 540 550
SSERLSAILK KLFEPNVSGD ATEEFVKAAA NGFAARCEEY LAGAAQPSTS
560 570 580 590 600
SASPSSGPDV NVNGVFAGHT ALQAASQNGH IEVIQVLLRH AVDVEIEDKD
610 620 630 640 650
GDRAVHHAAF GDEAAVIEIL AKAGADLNAR NKRRQTSLHI AVNKGHLNVV
660 670 680 690 700
KTLLTLGCHP SLQDSEGDTP LHDAISKEHD EMLSLLLDFG ADITLNNNNG
710 720 730 740 750
FNALHHAALK GNPSAMKILL TKTNRPWIVE EKKDDGYTAL HLAALNNHVE
760 770 780 790 800
IAELLVHMGK ANMDRQNVNL QTALHLAVER QHVQIVKLLV QDGADLNIPD
810 820 830 840 850
KDGDTPLHEA LRHHTLSQLK QLQDVEGFGK LLMGLRNANN KKASASIACF
860 870 880 890 900
LAANGADLTL KNRKQQTPLD LCPDPNLCKT LVKCYNERKT DDSELPGNVA
910 920 930 940 950
GTSSSARARA ASGSLNQSSS VNMPLSSLAA SSTFPAASSS SIFALNGIAN
960 970 980 990 1000
EMSQSLHEDP PKSSASLDEC LVCSDAKRDT VFKPCGHVSC CETCAPRVKK
1010 1020 1030 1040 1050
CLICRETVSS REKIDECLVC SDRRAAVFFR PCGHMVACEH CSALMKKCVL
1060 1070 1080 1090 1100
CRTQIDEILS FSLCCGGSGR PEKVSVAAGA MATVGLPLPD DRFMEAAAAA
1110 1120 1130 1140 1150
ACANASGHSV AMNNTVVTPV AGSSNQLNSQ NNLLAAAAAS SNVSNLSAAG
1160 1170 1180 1190 1200
NAMVAPSNVN NFQMDDVQKL KQQLQDIKEQ TMCPVCFDRI KNMVFLCGHG
1210 1220
TCQMCGDQIE GCPICRKTVE KRILLF
Length:1,226
Mass (Da):129,872
Last modified:July 5, 2004 - v3
Checksum:iA6D9212DC22211B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49551.3.
AY118647 mRNA. Translation: AAM50016.1.
RefSeqiNP_648826.2. NM_140569.2.
UniGeneiDm.10758.

Genome annotation databases

EnsemblMetazoaiFBtr0075520; FBpp0075275; FBgn0263601.
GeneIDi39750.
KEGGidme:Dmel_CG5841.

Similar proteinsi

Entry informationi

Entry nameiMIB_DROME
AccessioniPrimary (citable) accession number: Q9VUX2
Secondary accession number(s): Q8MSR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways