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Protein

E3 ubiquitin-protein ligase mind-bomb

Gene

mib1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta (Dl) and Serrate (Ser) receptors, which act as ligands of Notch proteins. Positively regulates the Notch signaling by ubiquitinating the intracellular domain of Dl and Ser, leading to endocytosis of Dl and Ser receptors. Regulates a subset of Notch signaling events, including wing margin specification, leg segmentation and vein determination, that are distinct from those events requiring neuralize (neur) activity. Also modulates lateral inhibition, a neur- and Dl-dependent signaling event, suggesting a distinct but partially complementary function with neur.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri173 – 22048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri970 – 100536RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1017 – 105236RING-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1183 – 121634RING-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: FlyBase
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • compound eye development Source: FlyBase
  • endocytosis Source: UniProtKB
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • lateral inhibition Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • positive regulation of endocytosis Source: FlyBase
  • positive regulation of Notch signaling pathway Source: UniProtKB
  • protein localization Source: UniProtKB
  • protein polyubiquitination Source: FlyBase
  • protein ubiquitination Source: UniProtKB
  • regulation of Notch signaling pathway Source: UniProtKB
  • sensory organ development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.
SignaLinkiQ9VUX2.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase mind-bomb (EC:6.3.2.-)
Alternative name(s):
Mind bomb homolog
Short name:
D-mib
Gene namesi
Name:mib1
Synonyms:mind-bomb
ORF Names:CG5841
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0263601. mib1.

Subcellular locationi

GO - Cellular componenti

  • apical cortex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi457 – 4571V → M in D-mib4; induces defects in Notch signaling. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12261226E3 ubiquitin-protein ligase mind-bombPRO_0000055951Add
BLAST

Proteomic databases

PaxDbiQ9VUX2.
PRIDEiQ9VUX2.

Expressioni

Tissue specificityi

Ubiquitous in the wing imaginal disk (at protein level).2 Publications

Gene expression databases

BgeeiQ9VUX2.
ExpressionAtlasiQ9VUX2. differential.
GenevisibleiQ9VUX2. DM.

Interactioni

Subunit structurei

Interacts with intracellular domain of Dl and Ser.1 Publication

Protein-protein interaction databases

BioGridi65061. 2 interactions.
IntActiQ9VUX2. 1 interaction.
MINTiMINT-865060.
STRINGi7227.FBpp0075275.

Structurei

3D structure databases

ProteinModelPortaliQ9VUX2.
SMRiQ9VUX2. Positions 109-164, 245-311, 523-871, 970-1005, 1017-1052, 1173-1226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 16869MIB/HERC2 1PROSITE-ProRule annotationAdd
BLAST
Domaini237 – 31579MIB/HERC2 2PROSITE-ProRule annotationAdd
BLAST
Repeati567 – 59630ANK 1Add
BLAST
Repeati600 – 62930ANK 2Add
BLAST
Repeati633 – 66230ANK 3Add
BLAST
Repeati666 – 69530ANK 4Add
BLAST
Repeati699 – 73133ANK 5Add
BLAST
Repeati735 – 76531ANK 6Add
BLAST
Repeati769 – 79830ANK 7Add
BLAST
Repeati802 – 83332ANK 8Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1159 – 118123Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 8 ANK repeats.PROSITE-ProRule annotation
Contains 2 MIB/HERC2 domains.PROSITE-ProRule annotation
Contains 3 RING-type zinc fingers.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri173 – 22048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri970 – 100536RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1017 – 105236RING-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1183 – 121634RING-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4582. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00790000122981.
InParanoidiQ9VUX2.
KOiK10645.
OMAiCYNERKT.
OrthoDBiEOG7WHH8P.
PhylomeDBiQ9VUX2.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010606. Mib_Herc2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00184. RING. 3 hits.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 3 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VUX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCAATLSSA KDSTNANASG GGGGGGGGGA PTNSNTNTNT NTQSTAVGVV
60 70 80 90 100
VSSAAGTGVG VGGGGGGGGS LPGGTTSSSS ASAAGGVAAG GGGNSAAALV
110 120 130 140 150
RRFSMEGVGA RVIRGPDWKW NKQDGGEGHV GTVRNFESAE EVVVVWDNGT
160 170 180 190 200
AANYRCAGAY DLRILDSAPT GVKHEGTMCD TCRQQPIFGI RWKCAECINY
210 220 230 240 250
DLCSICYHGD KHHLRHRFYR ITTPGGERTM LEPRRKSKKV LARGIFPGAR
260 270 280 290 300
VVRGVDWQWE DQDGGVGRRG KVNEIQDWSS ASPRSAAYVI WDNGSKNLYR
310 320 330 340 350
VGFEGMADLK VVNDAKGSNV YRDHLPLLGE NGPGKGPHGF QIGDKVTVDL
360 370 380 390 400
DLEIVQSLQH GHGGWTDGMF ECLSNAGMVV GIDEDHDIVV AYNSGNRWTF
410 420 430 440 450
NPAVLTKVSS PTTAPPEFQV GDIVKICSDV ESIKILQRGH GEWADAMQLT
460 470 480 490 500
LGKIGRVQQV YHDNDLKVEV GNTSWTYNPL AVCKVASSTA SDGSCAPVIP
510 520 530 540 550
SSERLSAILK KLFEPNVSGD ATEEFVKAAA NGFAARCEEY LAGAAQPSTS
560 570 580 590 600
SASPSSGPDV NVNGVFAGHT ALQAASQNGH IEVIQVLLRH AVDVEIEDKD
610 620 630 640 650
GDRAVHHAAF GDEAAVIEIL AKAGADLNAR NKRRQTSLHI AVNKGHLNVV
660 670 680 690 700
KTLLTLGCHP SLQDSEGDTP LHDAISKEHD EMLSLLLDFG ADITLNNNNG
710 720 730 740 750
FNALHHAALK GNPSAMKILL TKTNRPWIVE EKKDDGYTAL HLAALNNHVE
760 770 780 790 800
IAELLVHMGK ANMDRQNVNL QTALHLAVER QHVQIVKLLV QDGADLNIPD
810 820 830 840 850
KDGDTPLHEA LRHHTLSQLK QLQDVEGFGK LLMGLRNANN KKASASIACF
860 870 880 890 900
LAANGADLTL KNRKQQTPLD LCPDPNLCKT LVKCYNERKT DDSELPGNVA
910 920 930 940 950
GTSSSARARA ASGSLNQSSS VNMPLSSLAA SSTFPAASSS SIFALNGIAN
960 970 980 990 1000
EMSQSLHEDP PKSSASLDEC LVCSDAKRDT VFKPCGHVSC CETCAPRVKK
1010 1020 1030 1040 1050
CLICRETVSS REKIDECLVC SDRRAAVFFR PCGHMVACEH CSALMKKCVL
1060 1070 1080 1090 1100
CRTQIDEILS FSLCCGGSGR PEKVSVAAGA MATVGLPLPD DRFMEAAAAA
1110 1120 1130 1140 1150
ACANASGHSV AMNNTVVTPV AGSSNQLNSQ NNLLAAAAAS SNVSNLSAAG
1160 1170 1180 1190 1200
NAMVAPSNVN NFQMDDVQKL KQQLQDIKEQ TMCPVCFDRI KNMVFLCGHG
1210 1220
TCQMCGDQIE GCPICRKTVE KRILLF
Length:1,226
Mass (Da):129,872
Last modified:July 5, 2004 - v3
Checksum:iA6D9212DC22211B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49551.3.
AY118647 mRNA. Translation: AAM50016.1.
RefSeqiNP_648826.2. NM_140569.2.
UniGeneiDm.10758.

Genome annotation databases

EnsemblMetazoaiFBtr0075520; FBpp0075275; FBgn0263601.
GeneIDi39750.
KEGGidme:Dmel_CG5841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF49551.3.
AY118647 mRNA. Translation: AAM50016.1.
RefSeqiNP_648826.2. NM_140569.2.
UniGeneiDm.10758.

3D structure databases

ProteinModelPortaliQ9VUX2.
SMRiQ9VUX2. Positions 109-164, 245-311, 523-871, 970-1005, 1017-1052, 1173-1226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65061. 2 interactions.
IntActiQ9VUX2. 1 interaction.
MINTiMINT-865060.
STRINGi7227.FBpp0075275.

Proteomic databases

PaxDbiQ9VUX2.
PRIDEiQ9VUX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075520; FBpp0075275; FBgn0263601.
GeneIDi39750.
KEGGidme:Dmel_CG5841.

Organism-specific databases

CTDi57534.
FlyBaseiFBgn0263601. mib1.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4582. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00790000122981.
InParanoidiQ9VUX2.
KOiK10645.
OMAiCYNERKT.
OrthoDBiEOG7WHH8P.
PhylomeDBiQ9VUX2.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-DME-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.
SignaLinkiQ9VUX2.

Miscellaneous databases

ChiTaRSimib1. fly.
GenomeRNAii39750.
PROiQ9VUX2.

Gene expression databases

BgeeiQ9VUX2.
ExpressionAtlasiQ9VUX2. differential.
GenevisibleiQ9VUX2. DM.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010606. Mib_Herc2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00184. RING. 3 hits.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 3 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta."
    Lai E.C., Roegiers F., Qin X., Jan Y.N., Rubin G.M.
    Development 132:2319-2332(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DL AND SER, TISSUE SPECIFICITY.
  5. "Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila."
    Le Borgne R., Remaud S., Hamel S., Schweisguth F.
    PLoS Biol. 3:688-696(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF VAL-457.

Entry informationi

Entry nameiMIB_DROME
AccessioniPrimary (citable) accession number: Q9VUX2
Secondary accession number(s): Q8MSR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.